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Faculty of Biology and Biotechnology,
Maria Curie-Sklodowska University
Courses in English
Academic Year: 2013/14
Course title: Microbiology
Faculty/Department: Faculty of Biology and Biotechnology, Department of Genetics and Microbiology
Course title: Microbiology
Course code: MIRWM
Teacher: prof. dr hab. Wanda Małek ([email protected]) (48) 81 537 59 76
Lecture/Laboratory: 30 hrs / 60 hrs
ECTS credits: 8
Year of study: II (I0), winter semester
Educational and professional goals
The purpose of microbiology course is to familiarize students with the major groups of prokaryotes, their
cell structure, function, metabolism, and the role of microorganisms in the environment as well as
biotechnology. The student learns the techniques used to identify, differentiate microorganisms as well as
know the basic strategies and methods to combat pathogenic bacteria.
Course description:
the lectures:
Microbiology beginning. The world of microorganisms. The rules of contemporary classification of
microorganisms. Prokaryotic cell structure and functions. Endospores and other resting forms of bacteria.
Microbial nutrition: requirements for carbon, nitrogen, iron, phosphorus, sulfur, oxygen, hydrogen.
Nutritional types of microorganisms. Microbial growth: measurement of cell number and cell mass, the
growth in closed and continuous culture systems. Bacterial biofilms. Metabolism: aerobic and anaerobic
respirations, fermentations, chemosynthesis, photosynthesis. Control of microorganism growth by
physical and chemical agents. Bacteriophages: structure, lytic and lysogenic cycles. Economic and
environmental importance of bacteria.
the classes:
Comparison of different types of bacterial cell wall structure: Gram-negative, Gram-positive, acidresistant (Gram and Ziehl-Neelsen staining methods). Cytology of bacterial cell: the staining methods of
cell wall, genetic material, endospores, capsules. The microbiological techniques: isolation of pure
cultures, identification of bacteria on the basis of morphological and physiological features. Measure of
the bacterial growth. A types of microbiological culture media: preparation, characteristics. Control of
microorganisms: sterilization and disinfection. The effect of environmental factors on bacterial growth:
temperature, UV, osmotic pressure, pH, antibiotics. Interactions between organisms: mutualism,
antagonism. Identification of microorganisms in dairy products. Bacteriophages: isolation, bacteriophage
plaque-count assay, host range determination.
Literature:
“Biology of Microorganisms”- Michael T. Madigan, John M. Martinko, Jack Parker, Prentice Hall
International, Inc;
“Microbial Life”- Jerome J. Perry, James T. Staley, Stephan Lory, Sinauer Associates, Publisher
Sunderland, Massachusetts
Assesment method: oral or written exam
Prerequisities: basic knowledge of English
Primary target group: biologists, biotechnologists
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Course title: Biochemistry of Secondary Metabolites
Faculty/Department: Biochemistry Department, Faculty of Biology and Biotechnology
Course title: Biochemistry of secondary metabolites
Course code:
Erasmus subject area code:
Number of contact hours: 30 hours – lecture; 30 hours - laboratory
Course duration: I 20, summer semester
ECTS credits: 5
Course description:
The position of secondary metabolism in biochemical processes in living organisms and its regulation at
molecular and environmental level. The course covers the major groups of secondary metabolites in
microorganisms and plants, from the perspectives of biochemistry and biosynthesis with references to the
relationship between biological function of secondary metabolites in defense against different stress.
Characteristics of basic secondary metabolites (bacterial, fungal and from plants), their biosynthetic
pathways and practical applications for example such as pharmacological compounds.
Literature:
Dewick PM “Medicinal natural products. A biosynthetic approach.” John Wiley & Sons, LTD, 2002
Wink M (ed) “Functions and biotechnology of plant secondary metabolites” Wiley-Blackwell, 2010
Course type: lecture and practical laboratory
Assesment method: written examination, continuous assessment of laboratories
Prerequisities: completed course in biochemistry
Primary target group: students of biology, biotechnology, organic chemistry
Lecturer: dr hab. Anna Jarosz-Wilkolazka
Contact person: Anna Jarosz-Wilkolazka, Email: [email protected]
Phone: (48 81) 537 50 44
Deadline for application:
Remarks:
Educational and professional goals - student knows the main groups of secondary metabolites produced
by microorganisms and plants; can indicate a correlation between structure of secondary metabolites such
as alkaloids, terpenoids and antibiotics and their biological activities; is aware of chemical and biological
diversities of natural environment.
Course title: Medical Microbiology
Course title: Medical Microbiology
Teacher: Urbanik-Sypniewska Teresa, Wdowiak-Wróbel Sylwia (e-mail: [email protected]; [email protected])
Lecture/Laboratory, 30 hrs/ 60 hrs
ECTS credits: 8
Year of study 1st of 2º summer semester
Educational and professional goals
The course provides a basic theoretical and technical study of the structure, molecular biology,
pathogenesis, epidemiology, and laboratory identification of the various bacteria that cause human
diseases. The student can convert the acquired theoretical background knowledge into actual practice in
the course of experiments. University and government laboratories, research institutes, the pharmaceutical
and biotechnology industries, and diagnostic laboratories are employers looking for qualified
microbiologists.
Course description
Lecture:
Introduction to Medical Microbiology:
Normal Flora (human microbiome)
Gram-positive cocci: Staphylococcus, Streptococcus, Enterococcus
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Gram-positive spore-formers: Bacillus, Clostridium
Anaerobic infections: Bacteroides, Porphyromonas, Prevotella, Fusobacterium
Gram-negative bacilli: Enterobacteriaceae; Yersinia, Salmonella, Shigella, Escherichia, Coliforms,
Klebsiella, Proteus
Gram-negative bacilli oxidase positive Pseudomonas, Burkholderia, Acinetobacter
Gram-negative curved bacilli: Vibrio, Campylobacter, Helicobacter
Gram-negative coccobacilli: Brucella, Bordetella
Fastidious Gram-negative bacteria: Neisseria, Haemophilus, HACEK group infections, Legionella,
Coxiella
Animal-associated bacteria: Erysipelotrix, Francisella, Pasteurella, Mannheimia
Spirochetes: Treponema, Leptospira, Borrelia
Gram-positive bacilli: Listeria, Corynebacteria, Mycobacteria
Obligate intracellular bacteria: Rickettsia, Chlamydia
Cell Wall-less bacteria: Mycoplasma, Ureaplasma
Antimicrobial Chemotherapy
Laboratory: Isolation and culturing of bacteria. Microscopic examination of bacterial morphology by
different staining methods /Gram, Neisser, Ziehl-Neelsen. Examination and identification of selected
groups of pathogenic bacteria by using laboratory detection methods: molecular, morphological,
immunological and cultural. Examination of factors affecting disease spread. Detection of antibiotic
resistance mechanisms. Biochemical test for identification of bacteria /API-testing/. Preparation of
antibiograms. Determination of MIC and MBC. PCR methods for identification of pathogenic strains.
Required background: Biology course and/or General Microbiology course
Form of assessment: The written test exam composed of test questions e.g. True/False, Matching,
Multiple Choice and Short Answer
Course title: Plant physiology
Faculty/Department: Faculty of Biology and Biotechnology / Department of Plant Physiology
Course title: Plant physiology
Course code: FRRWM
Erasmus subject area code: 13.1 (Biology, plant physiology / electrophysiology)
Number of contact hours: 90 (lecture 30 hrs / laboratory 60 hrs)
Course duration: summer semester
ECTS credits: 8
Course description:
Issues for lecture: Water balance in plants, water uptake, transport and transpiration. Mineral nutrition of
plants. Structure, organization and function of photosynthetic apparatus, mechanisms and regulation of
the CO2 fixation and reduction. Carbohydrate metabolism and cellular respiration. Plant growth and
development, the role of phytohormones. Types and mechanisms of plant movements.
During laboratory classes students perform practical exercises concerning: water relations in plant cells
(diffusion, osmosis), measurement of transpiration intensity, analysis of mineral and organic compounds
of plants, isolation and properties of photosynthetic pigments, influence of light intensity and spectrum on
photosynthesis, gas exchange during plant respiration, plant growth, seed germination, plant movements
(phototropism, geotropism, photonasty).
Literature: Biochemistry & Molecular Biology of Plants, B. Buchanan, W . Gruissem, R. Jones (Eds),
American Society of Plant Physiologists, 2000.
Course type: lecture + laboratory
Assesment method: written exam
Prerequisities: basic knowledge of English
Primary target group: biology/biotechnology students
Lecturer: Prof. dr hab. Zbigniew Krupa
Contact person: dr Małgorzata Wójcik, email: [email protected], tel. +48 81 5375064
Deadline for application: Remarks: -
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Course title: Plant physiology (Basic course)
Faculty/Department: Faculty of Biology and Biotechnology / Department of Plant Physiology
Course title: Plant physiology (Basic course)
Course code: FRPAT
Erasmus subject area code: 13.1 (Biology, plant physiology / electrophysiology)
Number of contact hours: 60 (lecture 30 hrs / laboratory 30 hrs)
Course duration: summer semester
ECTS credits: 5
Semester:spring
Course description:
Issues for lecture: Water balance in plants, water uptake, transport and transpiration. Mineral nutrition of
plants. Structure, organization and function of photosynthetic apparatus, mechanisms and regulation of
the CO2 fixation and reduction. Carbohydrate metabolism and cellular respiration. Plant growth and
development, the role of phytohormones. Types and mechanisms of plant movements.
During laboratory classes students perform practical exercises concerning: water relations in plant cells
(diffusion, osmosis), measurement of transpiration intensity, analysis of mineral and organic compounds
of plants, isolation and properties of photosynthetic pigments, influence of light intensity and spectrum on
photosynthesis, gas exchange during plant respiration, plant growth, seed germination, plant movements
(phototropism, geotropism, photonasty).
Literature: Biochemistry & Molecular Biology of Plants, B. Buchanan, W . Gruissem, R. Jones (Eds),
American Society of Plant Physiologists, 2000.
Course type: lecture + laboratory
Assesment method: written exam
Prerequisities: basic knowledge of English
Primary target group: biology/biotechnology students
Lecturer: Prof. dr hab. Zbigniew Krupa
Contact person: dr Małgorzata Wójcik, email: [email protected], tel. 81 5375064
Deadline for application: Remarks: -
Course title: Mycology
Course title: Mycology
Teacher: prof. dr hab. Wiesław Mułenko (e-mail: [email protected])
Lecture/Laboratory, 15 hrs / 30 hrs
ECTS credits: 3
Year of study: I(Io), winter semester
Educational and professional goals:
Knowledge of the basic morphological and anatomical features of the fungal structure, distinguishing
them from other groups of organisms, and modes of reproduction and propagation in the environment
(specialist terminology). Knowledge of the role and importance of fungi in the natural environment;
protection of fungi as the natural components of the biosphere. Relationships between fungi and other
organisms – parasitism, commensalism, mutualism. Mycorrhiza, endophytes, lichens (lichenized fungi).
Ability to identify and distinguish the main edible and poisonous species and identification of symptoms
of diseases caused by fungi.
Course description
Structure of fungi and fungus-like organisms. Specific components of fungal cells. Vegetative and
generative fungal structures. Characteristic of selected representatives of Myxomycota, Oomycota,
Zygomycota, Ascomycota, Basidiomycota and anamorphic fungi. Morphology and anatomy of fungal
fruiting bodies. Types of asexual and sexual reproduction, sporogenesis, spore propagation. Trophic
forms. Fungal metabolites and their application. The role and importance of fungi in the ecosystem.
Modern criteria for classification of fungi, an overview of major taxa (The Code of Nomenclature).
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Required background: Form of assessment: Written examination (lecture) and test questions (laboratory)
Course title: General and Systematic Botany
Course title: General and Systematic Botany
Teacher: prof. dr hab. Wiesław Mułenko (e-mail: [email protected])
Lecture/Laboratory: 15 hrs / 60 hrs + 30 hrs (field classes)
ECTS credits: 8.5
Year of study: I(Io), summer semester
Educational and professional goals
Knowledge of the basic morphological and anatomical features of the plant structure, their classification
and modifications; knowledge of the role and importance of plants in the natural environment as the key
components of the biosphere; plant protection; adaptation to environmental conditions; the role of plants
in human life; plant use.
Skills to prepare microscope slides of various plant structures. Ability to identify the most important plant
species. Efficient use of appropriate equipment and keys for identification of organisms.
Course description
Plant cell structure. Forms of organization of plant organism. The structure and classification of tissues.
Morphology, anatomy, and modifications of vascular plant organs (root, stem, leaves). Flowers and
inflorescences – the structure, classification, modes of pollination. Fruits and seeds – development,
structure, classification, and propagation modes. Modes of plant propagation, alternation of generations.
An overview of major plant systems; the rules of botanical nomenclature, the code of nomenclature. The
main hypothesis of the origin and evolution of axial plants and thallophytes. A systematic overview of the
main strategies in plant development. An overview of systematic groups with particular emphasis on seed
plants as well as protected and crop species.
Required background: –
Form of assessment: Written examination (lecture) and test questions (laboratory); herbarium (after
field exercises).
Course title: Microscopic techniques
Faculty/Department: Faculty of Biology and Biotechnology, Comparative Anatomy and Anthropology
Department
Course title: Microscopic techniques
Course code: TEMBP
Erasmus subject area code: 13.1
Number of contact hours: 30
Course duration: II (II o), summer semester
ECTS credits: 3
Course description
The exercises enable to earn theoretical and practical knowledge from different kind of light microscopy
e.g. fluorescence, dark-field as well as confocal and electron-transmission and scanning. Main topics
include: construction of electron and confocal microscope, construction and operation on light
microscope-light - , dark-field, fluorescent microscope, preparation of specimens for electron microscopy:
trimming, cutting, contrasting of biological samples, grids observation under electron microscope.
Literature: Alberts B i in. PWN 2005, Zabel M. Immunocytochemia PWN 1999, Kłyszejko Stefanowicz L. Cytobiochemia PWN 2002, Cieciura L. Techniki stosowane w mikroskopii elektronowej.
PWN 1989
Course type: Laboratory: 30hrs
Assessment method: Continuous assessment in a form of tests- two tests during the course on which
final mark is based
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Prerequisities: The passed courses from cell biology, chemistry and biochemistry
Primary target group: students of biology
Lecturer: Pawlikowska-Pawlęga Bożena, PhD, Pawelec Jarek, M.S. Wydrych Jerzy, M.S.
Contact person: Pawlikowska-Pawlęga Bożena, PhD ([email protected]), 537-59-28; 537-59-16
Deadline for application: winter semester
Remarks: Classes are conducted with application of the sophisticated equipment: Leo– Zeiss 912 AB
electron microscope (Oberkohen, Germany), fluorescence microscope NIKON E-800 (Japan), microtome
RMC MT – XL (Tucson, AZ, USA), Confocal microscope LSM 5 PASCAL;
Educational and professional goals that the student achieves are as follows:
Graduate characterizes and explains chosen microscopic techniques, lists and characterizes stages of
preparation of biological samples, identifies particular cell organelles of the cells, employs techniques of
biological preparation including different microscopic techniques, documents the results of the
experiments,
Selects and applies microscopic techniques during observation and experiments.
Functional Human Anatomy
Course title: Functional Human Anatomy
Course code: AFRAG
Erasmus subject area code: 13.1
Number of contact hours: 30
Course duration: Winter semester
ECTS credits: 4
Course description: Microscopic observation of different tissues: epithelial, muscular, connective and
nervous. Macro- and microscopic structure of organs creating all the anatomical systems of human body.
The topics include: structure ,function and topography of skeletal, muscular, digestive, respiratory,
transport, urinary, reproductive, nervous and endocrine systems. Human tissues, elements of
anthropology.
Literature: Drake, Vogl, Mitchell “Gray’s Anatomy for Students”; Abrahams, Boom, Spratt, Hutchings
„Clinical Atlas of Human Anatomy”; Fenneis “Pocket Atlas of Human Anatomy”; Sobotta “Atlas of
Human Anatomy”.
Course type: laboratory
Assessment method: Continuous assessment in a form of written tests - several tests during the course
and final written test.
Prerequisites: Completed course in cell biology
Primary target group:
Lecturer: Dobrowolski Piotr PhD
Contact person: Dobrowolski Piotr, [email protected], +48 507 132 520
Deadline for application:
Remarks:
Course title: Basic techniques of cell and tissue culture
Faculty/Department: Faculty of Biology and Biotechnology/Department of Plant Anatomy and
Cytology
Course title: Basic techniques of cell and tissue culture
Course code: PHTRP
Erasmus subject area code:
Number of contact hours: 60 hrs
Course duration: Year of study: III (Iº), winter semester
ECTS credits: 6
Course description: Plant cell and tissue culture laboratory – basic equipment and organization of work.
The main techniques of sterilization and preparation of plant material. Components of culture media and
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preparation procedures. Hormonal control of cell growth and development. Organogenesis induction and
plant regeneration in cultured explants on solid media. Initiation and establishment of callus culture from
different types of explants. Meristem culture (isolation of shoot apical meristems). In vitro clonal
propagation of crop plants (method of micropropagation from axillary buds). Establishment of cell
suspension culture and its application in biotechnology.
Literature:
Evans D.E., Coleman J.O.D., Kearns A., Plant Cell Culture. BIOS Scientific Publishers, 2003
Smith R.H., Plant Tissue Culture. Techniques and Experiments. Academic Press, Second Edition, 2000
Course type: Laboratory
Assessment method: Written test questions
Prerequisities: Plant physiology, biochemistry, genetics
Primary target group: podstawowa grupa, do której kierowany jest kurs
Lecturer: Dr. Ewa Dziadczyk, dr hab. Ewa Szczuka, prof. UMCS
Contact person: dr hab. Ewa Szczuka, prof. UMCS, [email protected]
Deadline for application:
Remarks:
Course title: Methods of in vitro tissue culture
Faculty/Department: Faculty of Biology and Biotechnology/Department of Plant Anatomy and
Cytology
Course title: Methods of in vitro tissue culture
Course code: MKTBP
Erasmus subject area code:
Number of contact hours: 30 hrs
Course duration: Year of study: II (IIº), winter semester
ECTS credits: 3,5
Course description: Basic equipment and organization of work in plant cell and tissue culture laboratory.
Components of culture media and preparation procedures. Stages of preparation of sterile culture media;
proliferation of cell mass; hormonal development orientation (direct and indirect regeneration); somatic
embryogenesis; embryogenesis of haploid plants from microspores.. Hormonal control of cell growth and
development. Organogenesis induction and plant regeneration in cultured explants on solid media.
Initiation and establishment of callus culture from different types of explants. Meristem culture (isolation
of shoot apical meristems). In vitro clonal propagation of crop plants (method of micropropagation from
axillary buds). Establishment of cell suspension culture and its application in biotechnology.
Micropropagation; protoplast culture; microspore culture.
Literature:
Evans D.E., Coleman J.O.D., Kearns A., Plant Cell Culture. BIOS Scientific Publishers, 2003
Smith R.H., Plant Tissue Culture. Techniques and Experiments. Academic Press, Second Edition, 2000
Pollard J.W., Walker J.M. Plant Cell and Tissue Culture, Human Press 1990
Course type: Laboratory
Assesment method: Written and oral assessment
Prerequisities: Plant physiology, biochemistry, genetics
Primary target group:
Lecturer: dr hab. Ewa Szczuka, prof. UMCS, dr. Ewa Dziadczyk,
Contact person: dr hab. Ewa Szczuka, prof. UMCS, [email protected]
Deadline for application:
Remarks:
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Course title: Plant Embryology
Faculty/Department: Faculty of Biology and Biotechnology/Department of Plant Anatomy and
Cytology
Course title: Plant Embryology
Course code: EMRJB
Erasmus subject area code:
Number of contact hours: Lecture/Laboratory: 30 hrs /45 hrs
Course duration: Year of study: I (IIº), winter semester
ECTS credits: 6
Course description: Heterogenic cycle and sex determination in plants. Bryophytes: sporangium and
sporogenesis; archegonia, antheridia and gametes. Generative organs of ferns and horsetails. Heterospory
in club mosses and water ferns. Gymnosperms: pollen sac development, microsporogenesis, pollen grain
development, ovule development, macrosporogenesis, perisperm, archegonia, embryo. Angiosperms:
anther development, microsporogenesis, pollen development, types of: ovules, macrosporogenesis and
embryo sacs; pollination, progamic phase, fertilization, embryo, seed development. apomixis, GMO
Literature: 1. Wang T.L., Cuming A. Embryogenesis the generation of a plant. Bios 20062. Lersten N.R.
Flowering Plant Embryology. 2004
Course type: Lecture, Laboratory
Assesment method: Test (written examination), continuous assessment of laboratory
Prerequisities: Completed course in general and taxonomic botany
Primary target group:
Lecturer: dr hab. Ewa Szczuka, prof. UMCS
Contact person: dr hab. Ewa Szczuka, prof. UMCS ([email protected])
Deadline for application:
Remarks:
Molecular genetics
Name of course: Molecular genetics
Teacher: prof. dr hab. Teresa Jakubowicz ([email protected])
Lecture: 30 hrs
ECTS credits: 2
Year of study: II (II0 ), winter semester
Educational and professional goals:
The aim of the course is to introduce and discuss current topics related to molecular genetics of
eukaryotic organisms.
Course description:
Comparative genomics. Mechanisms of pre-RNA processing in eukaryotic cells. Types of splicing,
editing, catalytic RNA and DNA. Noncoding RNAs - biogenesis, physiological and pathological role.
RNA interference in vivo and therapeutic potential of RNAi. Epigenetic inheritance, epigenetic
mechanisms in human diseases. Genetic control of differentiation and development. Homeotic genes.
Cancer genetics. Chromatin regulation at mammalian telomers.
Required background: Completed courses of biochemistry (or molecular biology), genetics
Form of assessment: Written examination
Molecular mechanisms of adaptation
Name of course: Molecular mechanisms of adaptation
Teacher: prof. dr hab. Teresa Jakubowicz ([email protected]), dr hab. Małgorzata cytrynska
([email protected]), dr hab. Iwona Wojda ([email protected])
Lecture/laboratory: 30hrs / 45 hrs
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ECTS credits: 6,5
Educational and professional goals:
The aim of the course is to give students comprehensive view of molecular mechanisms of eukaryotic
organisms adaptations.
Course description:
Gene expression in humans and animals regulated by changes in environmental conditions. Gene and
genome rearrangement. Adaptive genes. Methods used for organisms changeability determination.
Adaptive character of immune response.
Required background: Completed course in genetics
Form of assessment:: Written examination
Course title; Invertebrate immunology
Name of course: Invertebrate immunology
Teacher: dr hab. Małgorzata Cytryńska ([email protected])
Lecture/Laboratory: 15 hrs/25 hrs
ECTS credits: 3.5
Year of study: I (IIo), summer semester
Educational and professional goals
Knowledge of essential mechanisms of invertebrate immunity. Understanding of differences between
vertebrate and invertebrate immune response.
Course description
Essential features of invertebrate immunity on the example of insect innate immunity. Self-non-self
recognition. Mechanisms of invertebrate immunity: anatomical and physiological barriers; cellular
response (types of hemocytes, phagocytosis, nodulation, encapsulation); humoral response (lysozyme,
phenoloxidase system, defense peptides and proteins). Regulation of defense peptide gene expression in
Drosophila. Immunoglobulin superfamily in invertebrates. Entomopathogenic organisms. Bioinsecticides.
Required background; Biochemistry, microbiology
Form of assessment: Written examination
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