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Gross anatomical study of crude drugs Clove DEPARTMENT OF TECHNICAL EDUCTION ANDHRA PRADESH Name Designation Branch Institute Year Subject Subject Code Topic Duration Sub topic Teaching aids : : : : : : : : M.Vasu Babu Lecturer in pharmacy Pharmacy GPW ,Guntur I D.Pharm. Pharmacognosy Ph -103 Gross anatomical study of crude drugs : 50 minutes : Clove (6/8) : PPT, Photographs Gross anatomical study of Crude Drugs Clove Objectives On completion of this period you would be able to know • Biological source • Microscopical characters • Chemical constituents & uses of Clove. Known to unknown Q 1. Did you ever see these? What are they ? Ans: Yes – they are cloves. Q 2. Did you ever used them in your house? Ans : yes. FIG 88.1 Q3. For what purpose, we use in our house ? Ans: As spice – used in food preparations. Q4. From which part of plant, is it obtained ? Ans: As flower bud. Q5. How do you find out the genuinity of this crude drug ? Ans: By observing Morphological / histological study. Cloves Biological source: Dried flower buds of Eugenia caryophyllus Family: Myrtaceae FIG 88.2 Microscopy T.S of microscopy of clove bud shows 1. Epidermis 2. Cortex 3. Columella FIG 88. 3 Epidermis • Single layered • Straight walled cells with anomocytic stomata • Covered with very thick cuticle FIG 88. 4 Cortex It shows 3 zones. Zone I Roughly radially arranged parenchymatus cells with 2 to 3 layers of big, ellipsoidal, schizolysigenous oil glands. FIG 88. 5 Cortex (contd) • Zone II Middle region containing a ring of bicollateral vascular bundles. • Xylem - composed of 3 – 5 lignified spiral vessels • Isolated pericylclic fibres (lignified) may be present around the vascular bundle. FIG 88. 6 Cortex Zone III • Zone of parenchyma composed of air spaces separated by lamellae. • one cell thick which supports the central columella. FIG 88. 7 Columella • Parenchymatus rich in calcium oxalate clusters . • A ring of vascular bundles towards the periphery. • Calcium oxalate prisms, Starch, Trichomes, Stone cells are absent. FIG 88. 8 Chemical constituents • Volatile oil (15-20%) Eugenol (70-90%) Eugenol acetate Caryophyllenes Small quantities of esters, ketones & alcohols • Tannins (10-13%) • Resins Galltannic acid • Chromone • Eugenin Uses • Dental analgesic • Carminative • Aromatic • Stimulant • Antiseptic • Flavouring agent Summary In this class we learnt about • Anatomical study • Chemical constituents • Uses of Clove Quiz Q 1.How many zones present in the cortex of Clove? a) 3 b) 4 c) 1 d) 2 Q 2.What type of stomata present in the Clove bud? a) Anomocytic b) Paracytic c) Diacytic d) Anisocytic Frequently asked questions 1.Write the monograph of Clove. 2.Explain the histological study of Clove. 3.Write the anatomical study of Clove. 4.Write the Biological Source, Family, Chemical constituents and Uses of Clove.