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Image Analysis Services
Particle Assessment
Investigating particle features
Vironova offers a unique range of objective TEM (transmission electron microscopy) image analysis services for qualitative and quantitative assessment of virus and virus-like particles (VPs and
VLPs). By utilising proprietary image analysis software Vironova can offer customers unique image
analysis services. The image analysis is objective, fully repeatable and uncovers particle features
undetectable to the human eye. In addition, Vironova performs statistical analysis comparing and
contrasting particles from the same or different samples. By combining diverse technical capabilities with virology and TEM expertise, Vironova offers a powerful quality assessment service of VPs
and VLPs used in for example vaccines, drug delivery or gene based medicine.
Sample
Morphology
Particles: Papilloma
Diameter: 51 nm
Capsid wall: 12 nm
Class: B
Content: none
Statistics
A
Classes
A
B
Count
36
9
Aggregation
0
0
Packaged
Empty
Characteristics
B
Example of analysis of a TEM image containing Papilloma virus. The marked particle is analysed separately in
the right hand column and aggregate statistics of all particles are displayed at the bottom.
Vironova AB (publ), Smedjegatan 6, 13134 Nacka, Sweden
Particle assessment services
By analysing several aspects of VPs and VLPs, Vironova provides customers with deep insight into their particles’ quality and characteristics in
the final product or after individual purification or production steps.
A standard particle assessment package includes the following studies of the material:
Size distribution
Most processes for expressing VLPs in cell cultures generate particles
of different sizes. Generally many particles are outside the desired size
range, which impairs the desired properties of the end product. This
service provides statistically reliable data on the number of particles and
their size distribution. For example the service can be used to evaluate
different expression protocols to optimise production.
Morphology assessment
This service provides customers with information on the homogeneity
of a sample or batch, and whether there are different particle populations present. This is of great importance to optimise features and to
ensure the desired effect of particles.
Packaging
Packing of VPs/VLPs for drug or gene delivery is never 100% successful.
TEM is an excellent tool to observe the particles as a population and to
measure the full-to-empty particle ratios. This is often done manually by
ocular counting, a cumbersome and subjective process. Vironova’s service provides customers with objective data on the degree of packaging for each particle and on the distribution of packed and unpacked
particles in the batch. This is valuable information for evaluating the
efficacy of the production protocol.
Aggregation
Aggregation inhibits the efficacy of particle based vaccines or viral
vectors. This service gives valuable insight into the degree of aggregation in all steps of production. Particle aggregation is often influenced by the physical conditions of the medium. With this service the
optimal buffer conditions can be determined to avoid aggregation.
Broken particles and/or cell debris
Purification of VPs/VLPs is a trade-off between preserving as many particles as possible and having as little debris as possible. This service helps
our customers monitor the amount of broken particles and cell debris
in relation to intact particles.
Vironova AB (publ), Smedjegatan 6, 13134 Nacka, Sweden
Purchase of image analysis services
Regardless of the application for VPs of VLPs, Vironova’s services add value to the research, development and production. Our experience and knowledge in TEM and image analysis grant high
quality results.
All image analysis services include TEM imaging and are produced on demand to fully capture
each specific research question.
Please contact Vironova for quotes and more information on image analysis services.
Vironova’s proprietary image analysis tools
Limitations of the human eye, images with noisy backgrounds and viewers’ different scientific
background make manual analysis of TEM images of viruses a tiresome and difficult process that
produce subjective results. Vironova has developed unique, automated image analysis tools to
detect and analyse VPs and VLPs in highly textured TEM images. The technology adds speed, accuracy, repeatability and objectivity to the process of analysing TEM images.
Other services offered by Vironova
Vironova also offers basic electron microscopy imaging services. New digital camera technology
has vastly improved the capabilities of electron microscopy imaging in life science applications.
High quality digital images can be captured and managed within seconds. Vironova specialises
in digital imaging of virus, virus-like and nano particles, and has the extensive experience needed
to provide high quality images of nano scale structures. The service covers several preparation
and labelling techniques and gives customers valuable insight into VP and VLP research, development and production. Please contact Vironova for quotes and more information on imaging
services.
Contact
Please use any of the following contacts to request more information on services, pricing
and how to order:
Web: www.vironova.com
E-mail: [email protected]
Phone: +46-(0)8-702 67 90
About Vironova
Based in Stockholm, Sweden, Vironova AB (publ) is a leading developer and innovator of
antiviral drugs and virus analysis & identification products based on transmission electron
microscopy (TEM) images. Read more at www.vironova.com.
Vironova AB (publ), Smedjegatan 6, 13134 Nacka, Sweden