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Infection Control Exam Preparation Course Outline -2017 Catherine A. Collier RDH, B.Ed, MS The contents of this handout are intended to provide insight into the information that may be presented on the IC Exam. This information has been selected by Catherine Collier, RDH, B.Ed, MS. Course participants are responsible for researching additional course information reflecting this handout content. The author, Catherine Collier, DOES NOT grant permission to copy or use this course information for teaching purposes at any level other than course participation directly affiliated with either Professional Learning Services or Catherine Collier. Participants are required to print out and bring this handout to their scheduled course. All content will be addresses on the day of the program. The Infection Control Exam Blueprint 10% Patient and Dental Healthcare Worker Education 20% Standard/Universal Precautions and the Prevention of Disease Transmission 10% Maintaining Aseptic Conditions 15% Instrument Processing 15% Aseptic Procedures 30% Occupational Safety Agencies Involved: The following agencies constitute who selects and enforces the regulations regarding Occupational Health and Safety for dental healthcare workers. 1. Occupational Health and Safety Administration - OSHA OSHA compliance is mandatory for all institutions OSHA is the law regulator and authority OSHA is concerned with the health and safety of the employee 2. The Center for Disease Control – CDC Centers for Disease Control and Prevention Federal agency that investigates, identifies, reports, tracks and tries to prevent and control diseases CDC is a part of the Department of Health and Human Services CDC is a nationally recognized agency regarding issues concerning public health, e.g., workplace hazards, infectious diseases, environmental health threats. CDC establishes the guidelines and recommendations for infection control policies and procedures CDC is part of the public health department, Its main concern is the health and safety of the patient 3. Environmental Protection Agency - EPA Federal agency that works to control and abate pollution in air, water, solid waste, pesticides, disinfectants, radiation and toxic substances. 4. Food and Drug Administration - FDA An agency of the Department of Health and Human Service that is responsible for ensuring the safety and effectiveness of food, drugs, cosmetics and medical devices 1 Who is responsible to provide employee training: The owner of the dental practice is responsible to provide training Training should be conducted: Annually for all employees For new employees prior to initial assignment When a new product or new equipment is introduced into your practice Office training can be conducted in various ways Purchase a DVD for team to view Appoint a safety coordinator in your practice to train Hire a person who is extremely familiar with the guidelines of OSHA and CDC ETC. Attend a presentation or have an in office presentation and walk through assessment of equipment and supplies Office Record Keeping For Employees Employee medical or accident reports records are kept for full, part time or former employees A medical record is any record that holds the health status of an employee Record is completed by a doctor or healthcare provider Employees have the rights to access their record Employees are entitled to a copy of their medical records Employee records also include, but are not limited to: CE credits – valid for 3 years Certification courses License Malpractice Insurance Any certification the employee carries Records of required immunization or decline Results of medical testing, medical opinions and treatment Employers should inform outside services of office policies and expect temps, interns or outside services to comply with office policies How to handle an office inspection: Ask inspector to present credentials The following process should occur with an inspector: Opening conference Walk through assessment of the facility – Office Investigation Closing conference reviewing the office findings Results of inspection / Practice owners have the right to appeals findings How to file for work related injuries Work related injuries are considered accidents or injuries that occur in the workplace during the days and times employees are assigned to be there. They are injuries that require the employee to seek medical attention outside the office. 2 Employers must establish a log for each facility OSHA Log 300 will assist the employer and employee with how to properly recognize and file a work related accident. Log 300 classifies work-related injuries and illness Log 301 The complete injury and illness Report Log 300A Summary Report Post from Feb. -April Documentation and Policies The record is legible to someone other than the writer Entries should be written in black or blue ink Do not skip lines, erase or white out info or changes All entries should be dated Sign all documents with full name and credentials Document patient acceptance and refuse of treatment Document any and all emergency situations that may occur for patient or employee Document treatment received or refusal of post exposure or accident for patients or employees Remember.. If you don’t write it down .. You never did it Occupational Exposure Control Plan - OSHA CFR 1910.1030 – Employers are required by OSHA to have a written Exposure Control Plan. An Exposure Control Plan protects workers from exposures to blood and other body fluids. ECP reduces exposure incident costs ECP serves as a document to answer questions related to bloodborne pathogen Exposure Control Plan should include the following information: List the current names of high or low risk employees Must state their job classification and task descriptions Must state the methods of compliance Employees should receive training according to their job position and potential risk exposure Employee Tasks and Work Restrictions Employers should provide a comprehensive written policies manual regarding employees work restrictions Work restrictions/exclusions are limitations in professional duties as determined by policies set within the dental practice setting Restrictions/exclusions may be imposed on dental workers whose health status poses a high risk of infection to patients, coworkers, or themselves Every dental practice should have a written work restriction and exclusion policy which includes who may implement restrictions and exclusions Hazard Communication Program Occupational Safety and Health Administration (OSHA) guideline requiring dental offices to develop and implement a program to instruct all employees who might be exposed to hazardous materials in the safe handling and disposal of those materials. These materials include but are not limited to: Amalgam Composites Glass Ionomers Disinfectants Bleach Phenols/ alcohols Impression materials Developer/ Fixer 3 Injectable/ OTC Safety Data Sheet (SDS) Information provided by manufacturers of potentially hazardous products that must include: Product identification Chemical content Hazardous ingredients Physical data, fire and explosion information Reactivity data Health hazard data Emergency and first aid procedures, spill or leak procedures Labeling and storage information. The New Hazard Communication Standard was previously known as "Employee's Right to Know,“ Globally Harmonized System of Classification and Labeling (GHS). Allows employees to understand the risks associated with and protections needed when working with hazardous chemicals or drugs. Required updates to chemical labels including the use of pictograms and signal words. Material Safety Data Sheets will be updated and be referred to as Safety Data Sheets. The purpose of SDS is to provide information on: The United Nation action on hazardous chemicals Hazardous Chemicals in the workplace Upkeep of equipment and supplies Injuries that occur in the workplace The user of the chemical product assumes liability for any misuse of products Follow manufacturer’s directions for: Proper mixing Use/handling Storage and disposal Proper selection of personal protective equipment Effective ventilation Secondary Container Labels Information Dental Unit Waterlines Bacterial growth in the dental unit waterline is encouraged due to: Size of tubing Surface material Speed of flow rate Intermittent flow rate The rod- shaped, gram-negative bacteria Legionella pneumophilia is among the highest bacterial growth found in the waterline. These bacteria are considered to be the cause of Legionnaires’ disease. Available methods for treating the dental unit waterline: 4 Utilize Isolated Water Supply - Isolates the dental unit water from the municipal water supply. Water supply is filled and maintained by office staff Utilize Chemical or Physical Treatments of the water supply Flushing the waterlines for 20-30 seconds clears the waterlines of free-floating microorganisms Utilize sterile water supply Pros and Cons of having an isolated water supply Isolate the dental unit from the municipal water supply Allow the practice to control the quality of water in the unit Provide a way to introduce chemical agents to waterlines and permit the use of water of known microbiologic quality Cannot reliably improve the quality of dental unit water without additional chemical or mechanical treatment against the biofilm Improperly maintained systems could deliver water of worse quality than from a municipal source. Pros and Cons of Chemical treatment of waterline May be time-consuming and technique-sensitive Treatment involves purging the waterlines, adding a chemical to the water reservoir Require strict compliance with the recommended treatment regimen May be incompatible with dental equipment (the proper treatment protocol depends on the type and components of the dental unit) Continuous-release chemicals may be incompatible with various dental materials (for example, dental Adhesives) Pros and Cons of utilizing physical treatments Produces water that meets or exceeds drinking water standards May include anti - retraction features Are not labor intensive when placing and replacing May require periodic chemical treatment of the post-filter segment of the waterlines to control biofilm May be expensive, as each waterline requires a separate filter that must be maintained daily or weekly, depending on the brand Point of entry filters are a considerable investment and must be professionally installed Pros and Cons of flushing the waterlines Helps to remove contaminants that may have been retracted during patient treatment Clears away free-floating organisms in the water, temporarily reducing the number of microbes in treatment water Flushing is not recommended as a control method for dental water quality Has only temporary effects (Biofilm bacteria continually break free and re-contaminate dental unit water during the course of clinical treatment) When used alone, has little effect on waterline contamination Does not prevent or eliminate biofilm Pros and Cons of utilizing sterile water delivery systems Autoclavable, self-contained water systems with disposable or autoclavable tubing Bypass the dental unit's water line Deliver sterile water to the patient Used for oral surgery and implantology handpieces, ultrasonic scalers and retrofit devices for restorative handpieces Have autoclavable components which require sterilization between uses Radiation Exposure Controls Provide film badges Keep records of the test results Provide lead aprons, goggles, gloves as needed Dispose of lead aprons according to the EPA regulations Radiation Warning Signs: 5 Applies to facilities with x-ray machines Survey the types of radiations used in the facility Restricted areas to limit employee exposure Rooms and equipment may need to be labeled with caution signs Infection Control Practices and Policies Goals of Infection Control is to break the chain of disease transmission In order to stop the spread of an infectious disease dental healthcare workers need to : Maintain host resistance Reduce or kill the number of organisms Stop the path of an organism before it enters a host Minimize or eliminate contact with blood and all body fluids Infection Occurs when there is a place for an organism to live Mode of Transmission - a way to transfer the organism to the host Causative agent - the presence of a dangerous organism Susceptible Host – Employee is susceptible Groups of Bacteria Include: Gram negative bacteria – stain pink or red Endotoxins – are a toxic substance in gram-negative bacteria Gram Positive bacteria stain blue or purple Shapes – Cocci- spherical cells Bacilli - Rod-shaped cells Spiral - curved shaped cells Endospores – one of the most resistant dormant structure formed inside bacterial such as Geobacillus, Bacillius, and Clostridium Factors which contribute to bacterial growth include: 1. Oxygen Obligated Aerobes – need oxygen to grow Obligated Anaerobes – cannot tolerate oxygen 2. Temperature – Thermophiles 132 degrees F –158 degrees F, Most resistant to heat is G. Stearothermophilus Mesophiles 71.6 degrees F – 133 degrees F Psychrophiles 33.8 degrees F- 71.6 degrees F 3. Acidity 0 = high acidity – 14 high alkaline Neutral is a pH of 7 - it is a balance between acid and base 4. Nutrients - bacteria need proteins, polysaccharides, lipids and nucleic acid such as RNA/DNA to grow Source of Bacteria 6 1. Endogenous infections are caused by microorganism usually on the surface or inside the body Ex. Caries, periodontial disease, 2. Exogenous infections are caused by microorganisms that contaminate the body form an outside source Ex. HBV, Strep throat, measles, chickenpox Disease transmission occurs: Indirect contact with blood or OPIW Indirect contact with contaminated objects When mucous membranes contact splatter Breathing airborne particles Parenteral Exposure Airborne Disease Influenza – “The Best Mutator” Symptoms of Influenza including 2009 H1N1 influenza, may have some or all of these symptoms: Fever - not always Cough Sore throat Runny or stuffy nose Body aches /Headache/ Chills Fatigue Sometimes diarrhea and vomiting The flu can infected and re-infected Type A Usually present in larger epidemics Type B Accounts for most hospitalizations Type C Mostly respiratory Illness Virus can drift and subdivide thus making immunity difficult Elimination of potential exposure to influenza includes: Deferral of treatment Mask coughing individuals Identify patients with influenza-like illness at check-in Offer a facemask or tissues to symptomatic patients Follow respiratory hygiene/cough etiquette Reschedule non-urgent care – at least 24 hrs. wo/ fever or medications Specific Recommendations for Dental Health Care Encourage seasonal influenza and 2009 H1N1 influenza vaccinations. Consistent implementation of sick-leave policies and vaccination Personal protective equipment (PPE) Self-assess daily for symptoms Stay home if ill or have family member that is ill Separate ill patients from others whenever possible if evaluating for urgent care. Patient-reminder calls to identify patients reporting influenza-like illness Viral Infections: 7 Herpes Virus “The Houdini of Viruses” Common properties include: DNA coding Similar architectural structures Can remain dormant for the life of the host Most Common Strains of Herpetic Viruses 1. 2. 3. 4. 5. 6. 7. 8. Herpes labilias - Human herpes virus 1 (HHV1) (HSV1) Genital herpes - Human herpes virus 2 (HHV2) is also called herpes simplex virus 2 (HSV2) Varicella-Zoster - Human herpes virus 3 (HHV3) causes chickenpox. Can cause a recurrent viral infection such as herpes zoster or shingles Epstein-Barr Human herpes virus 4 (HHV4) Major cause of infectious mononucleosis Human herpes virus 6 (HHV) and 7 (HHV7) HHV6 and HHV7 are so common that most of humankind has been infected at some point HHV7 can also cause roseola Human herpes virus 8 (HHV8) - Discovered in Kaposi's Sarcoma , Associated with AIDS Hepatitis A “The Salad Bar Disease” Spread of filth or unsanitary conditions Mostly related to socioeconomic development Younger patient is usually asymptomatic, but can develop self-immunity to virus Self- limiting disease - 1% of patients require liver transplant Four inactivated vaccines are internationally available Vaccines are safe and effective, long-lasting Vaccines are NOT licensed for children less than one year of age. Hepatitis B Is spread via percutaneously or permuscosally by infected body fluids It can be passed on through birth – If mother is HBsAG and HBeAg there is a 70% change of the infant contracting HBV Sexual activities Contact with contaminated needles or sharp objects Hepatitis C “The Silent Epidemic” There is no vaccine for HCV The virus can live in body 20 to 30 years A small number of people with hepatitis C infection may develop liver cancer Liver failure may occurs as a result of severe damaged by hepatitis C Monitoring the Condition of The Liver Who should be tested for HCV 8 Illicit drug use Unexplained or unusual liver function tests Babies born to mothers with hepatitis C Health care and emergency workers who have been exposed to blood or accidental needle sticks Hemophiliacs who were treated with clotting factors before 1987 Long-term hemodialysis treatments Blood transfusions or organ transplants before 1992 Sexual partners of anyone diagnosed with hepatitis C infection Immunization CDC Healthcare Vaccine Recommendation Measles, Mumps , and Rubella – MMR Varicella – Chickenpox Tetanus, Diphtheria and Pertussis –Tdap All dental Healthcare workers receive a baseline tuberculosis screening upon hiring regardless of the risk classification of the facility Flu vaccine is offered to increase prevention Hepatitis B Vaccine Provided during professional training Offered to workers likely to contact blood or OPIW Serologic test not necessary prior to vaccine Needle stick exposure for unvaccinated person should lead to initiation of HBV Immune Globulins are not a substitute for the vaccine Always Report reactions to the vaccinations Mild reactions such as soreness, headaches, and fever are common side effects of the flu vaccine Severe reaction such as difficulty breathing, hives, or facial swelling, seek medical attention immediately. Post Exposure Evaluation Report all incidents and accidents immediately to supervisor Record all necessary data required for testing Employees must receive a medical evaluation and follow up Paper work must be completed and ready to go to evaluation facility Source testing / employee testing If needed post exposure treatment should begin within 2 hours of incident Needle stick injuries Are Most likely to occur: Organize a product selection and evaluation team Collect information from a dental “Safety Syringe and Needle Screening” form Set priorities for product consideration Safe handling of the device should be demonstrated by all employees that are required to use it Needles and syringes are single patient use only Rubber septum should be disinfected with alcohol prior to piercing Sharps Safety: Prepare the patient and organize the work area with prevention in mind. Keep exposed sharps in view and under your control. Visually inspect for unprotected sharps in trays and waste receptacles. Be responsible for the sharps you use. Activate safety features. Dispose in sharps containers. What to do if you suspect puncture: 9 Remove glove immediately and fill it with water to check for penetration Rinse with cool water and cover REPORT IMMEDIATELY TO SUPERVISOR Use OSHA form 300 to record and report Report all incidents and accidents immediately to supervisor Record all necessary data required for testing Employees must receive a medical evaluation and follow up Paper work must be completed and ready to go to evaluation facility Source testing / employee testing If needed post exposure treatment should begin within 2 hours of incident Utilize Bio-Hazard Stickers on the following items: On bags/containers of contaminated laundry On bags/containers of regulated waste On refrigerators and freezers that are used to store blood or OPIM On bags/containers used to store, dispose of, transport, or ship blood or OPIM (e.g., specimen containers) Sharps containers Blood soaked gauze containers When dried blood can flake off from a disposed item When there are bloody gloves and sharps disposed in the container Disposal of Anesthesia Carpules: Containing visible blood are considered as sharps medical waste. Empty, unbroken without blood or anesthetic can be placed in trash for general waste disposal. Broken with no visible blood or anesthetic are technically not medical waste but should be considered for sharps container With remaining anesthetic should be disposed of in a container properly labeled for transportation of pharmaceutical waste to a medical waste incinerator Use appropriate gloves for breakdown and disposal of sharp items and needles. Place contaminated items in an appropriate leak-proof biohazards container. Procedures and Prevention of Disease Transmission Hand Hygiene - Resident and Transient Bacteria Resident skin flora consist of microorganism that colonize the skin and become permanent residents Can never be totally removed, but their numbers can be reduced Resident flora can cause infection they are less likely to than transient Transient Bacteria contaminate hands come from contact with contaminated surfaces They do not live long or colonize or live long Transient bacteria can contain disease producing microorganism Proper Handwashing effectively removes transient bacteria and reduces resident bacteria. Your skin serves as your biggest barrier of protection and is compromised if: Chapped, presence of dermatitis, abrasions or open wounds Hands are washed too frequently By exposure to hand care products and dental materials Soaps, lotions and gloves should be compatible Effective Hand Hygiene Protocol: 10 Non-surgical hand washing = 15 seconds When to wash hands with plain or antimicrobial soap: Prior to putting on or after removing gloves Before surgery to remove soil prior to using an alcohol hand rub Before and after eating After using the restroom When hands are visibly dirty or contaminated, or when they are visibly soiled with blood or other body fluids When to wash hands with an antimicrobial soap Surgical handwashing = 2 minutes -includes arms Surgical hand scrub/soap antisepsis should be performed before surgical procedures Use either antimicrobial soap, or a combination of non-antimicrobial soap and water followed by an alcohol-based surgical hand rub The surgical hand scrubbing process differs significantly from the other hand hygiene procedures Surgical hand soaps should contain one of the following ingredients: Chlorhexidine, Iodine, Iodophors, PCMX. All are broad spectrum, persistent and fast acting. Alcohol based hand rubs - 60%-95% ethanol or isopropanol alcohol. Should be considered in addition to the surgical scrub. Also use if water-boil advisory is in effect. Hand rubs do not contain detergents – 30 seconds Selection of Personal Protective Equipment (PPE) Personal Protective Equipment: Should Fit properly Do not touch or adjust during procedure Be removed and disposed of if visibly soiled or torn Be worn in work related areas Be disposed of according to waste regulations When wearing PPE also consider: Do not eat or drink Do not applying cosmetics Do not handle contact lenses 1. Gloves Gloves do not replace hand washing They should fit properly to reduce cramping and possible injury Gloves are for single use only, reuse and washing is unacceptable Various reactions to glove materials include: Irritant contact dermatitis is common among dental workers. Irritant contact dermatitis is caused by physical irritation of the skin. It presents as dry, itchy, irritated areas of skin around the area of contact with the offending agent 11 Allergic contact dermatitis is a skin condition that can result from exposure to chemicals such as methacrylates, glutaraldehydes and rubber manufacturing chemicals; this type of dermatitis often appears as a rash beginning several hours or even days after contact Any condition which compromises healthy skin increases the risk of percutaneous exposure to blood and body fluids. In both cases, contact with patients should be limited and policies in place for appropriately diagnosing, treating and managing conditions Work restriction for employee with glove related reactions include: Seek diagnoses for latex allergy and determine course of treatment. Implement strategies that will reduce the use and contact with latex in the dental setting. Latex hypersensitivity - level 1 is a potentially life-threatening allergy to the proteins contained in natural rubber latex. Latex allergies are serious as they include a whole body reaction within minutes of exposure. Common reactions include runny nose; sneezing; itchy eyes; scratchy throat; hives; and itchy, burning skin sensations Prevention of Latex Hypersensitivity include: Competent patient medical history/ food allergies Employees require testing to provide proof of latex allergy Use powder free, latex free or low allergen latex products Schedule patient in early part of the day 2. Mask and Face Shields When selecting a mask for dental procedures consider the following features: 1. 2. 3. 4. 5. 6. Fluid Resistance Submicron Particle Filtration Efficiency Bacterial Filtration Efficiency Breathability Flame Spread Mask selection is by procedure type Bacterial filtration of 95% for a particle range of 3 µm to 5 µm in diameter Mask should fit firmly around nose and mouth, no side openings Always remove from elastic band or ear loops Mask should be worn when wearing a face shield 3. Gowns 50/50% cotton polyester 60/40% fluid resistant Long sleeves and high collar, use caution with scrubs Fluid resistant gowns release debris on contact Cotton polyester materials absorb matters and require laundering services 4. Protective Eyewear OSHA requires employers provide safety glasses It is the responsibility of the practitioner to use vigilantly and safely Clean eyewear according to manufacturer’s instructions Hand pieces could cause irreversible damage. 12 Rotate at speed of 180,000 to 500,000 times per minute, Can project particles at speeds up to 50 miles per hour. Implement Work Practice and Engineering Controls Work practice controls - Incorporates behaviors that serve to reduce injury or exposure Single handed recapping Incorporate cheek retractors Immediate removal of burs at point of use Sharp containers at point of use Engineering Controls - The use of devices that eliminate or isolate a hazard Needle recapping device – REVIEW ANNUALLY Sharps container Ultrasonic cleaners / Instrument washer Instrument cassette Preparing the operatory for patient care includes : a. Review medical history and note any restrictions. Check for any current of infectious diseases or allergies. If necessary, postpone treatment because of a health issue. b. Use a pre-procedural mouth rinse containing either Chlorhexidine gluconate, Essential oils, or Povidone-iodine. Pre-Procedural Mouth Rinses should contain antiseptic properties and have residual activity to reduce the number of microorganisms: On the surface of oral tissue Released through aerosols, spatter or direct contact Introduced in the patient's bloodstream during invasive procedures Pre-procedural mouth rinses should be unit dosed and given to patients in a disposable cup c. Clean and disinfect all clinical work surfaces in the operatory. Utilize barriers and surface disinfectants Disinfection The process of killing pathogens by physical or chemical means; it does not destroy spores and resistant viruses. Surface disinfectants should be EPA approved and meet the following standards: 13 Broad spectrum TB kill claim – HIV/HBV kill claim Time efficient Contact time vs. Kill time Easy and safe to use Shelf life vs. Use Life d. Utilize single - use items as much as possible. e. Keep housekeeping surfaces, such as floor and walls clean and sanitize f. Dispose of routine waste in lined receptacles in operatories g. Discard sharp related itme into sharps container in operatory Preparing instruments for sterilization Transportation of Instruments Transport instruments to sterilization center in cassette, covered tray or instrument bin Pre-cleaning Pre-cleaning instruments is a critical step in processing instruments for sterilization. Proper fitting utility gloves should be worn by employee when handling contaminated instruments. Instruments should be places in an ultrasonic unit or unti designed to remove bio-burden load on instrument prior to sterilization. Use recommended ultrasonic solutions and test ultrasonic cleaner routinely to make sure it has the proper cavitation strength to remove blood and bio-burdens. Proper pre-cleaning of instruments includes: Pre - soak items immediately or clean Do not allow to remain unattended Dried blood and body fluids make cleaning difficult and increases the possibility of an exposure incidence Wearing utility glove, lift the handles of the basket in the ultrasonic unit. Allow ultrasonic solution to drip back into the bid. Then rinse instrument, pat dry and prepare for packaging. Instruments. Packaging Instruments Use FDA approved materials All packaging material should have an internal and external indicator Make sure cassettes are closed and instrument tips are not sticking out Cassettes must be properly wrapped Avoid placing heavy instruments in bags Avoid using closed containers or staples Close pouched at the designated fold location Avoid using paper/ plastic bags in dry heat sterilizer Instrument Sterilization Sterilization is a process that destroys all forms of microorganisms, including bacterial endospores. Classification of Instruments for Sterilization: 14 Critical – penetrates the mucous membrane requires sterilization. Have the greatest risk of transmitting infection are Semi-critical – touches the mucous membrane requires sterilization or a minimum of HLD Non- critical - touches intact skin such as blood pressure cuff or x-ray head Requires HLD Methods of Sterilization Steam Sterilization gravity displaced, vacumn pump, positive steam flush 20-30 minutes 121F - 250 degrees F –Geobacillus Stearothermorphilus Dry heat sterilization – Static air 60 – 120 minutes at 320 - 375degrees degrees F Bacillus atriophaeus Unsaturated Chemical Vapor sterilization Chemclave – 20 minutes at 270 degrees F – Geobacillus Stearothermorphilus Active ingredient is formaldehyde 0.23% MONITORING STERILIZER Mechanical - Record temp and pressure Chemical - Use internal and external indicators Biological - Spore testing CDC recommends weekly testing Test when implant device is being sterilized When using a new packaging device or material After sterilizer has been repaired After sterilizer has been moved or relocated If test results indicate a failed cycle take the unit out of use until problem for failure has been determined All packages should be stamped with the date of sterilization. Facilities with more than one sterilization unit should have a system in place for identification of the unit used for sterilization. Unloading and storage of instruments Allow instruments to dry and cool completely in sterilizer Do not touch moist packages with bare hands Do not store wet packages in drawers or cabinets. Stored all instruments wrapped unless they are for immediate use Do not store near contaminated items Instruments in bags stay sterile indefinitely Proper disposal of waste 15 Always check state and local regulations All waste receptacles must be lined General waste – PPE, slightly soiled cotton or gauze, barriers Blood and saliva – dispose in sanitary sewer Tissue – sanitary sewer Teeth – sharps if no amalgam, or disinfect and give to patient Operatory trash – regular waste 16