Download Directions for Use HistoChoice® MB (Molecular Biology) Tissue

Directions for Use
HistoChoice® MB (Molecular Biology) Tissue Fixative
Code
Description
Size
H120-100ML
HistoChoice® MB (Molecular Biology) Tissue Fixative
100 mL
H120-1L
HistoChoice® MB (Molecular Biology) Tissue Fixative
1L
H120-4L
HistoChoice® MB (Molecular Biology) Tissue Fixative
4L
General Information
VWR Life Science AMRESCO’s HistoChoice® MB (Molecular Biology) Tissue Fixative is
specially formulated to preserve antigenic sites (for antibody probes) and nucleic acid sites (for
in situ hybridizations) in their native state, rendering pre-digestion or other recovery procedures
for these important sites unnecessary. Primary antibodies can often be diluted several-fold due
to the increased number of preserved antigenic sites, saving cost on every slide that is
processed. HistoChoice® MB provides superior sensitivity, allowing markers to be observed at
lower antibody levels. It makes an excellent transport medium and can be safely washed down
the drain upon neutralization in most facilities. HistoChoice® MB directly replaces common
fixatives, including formaldehyde-based, alcohol-based, Zenker's, B5 and B3, and Bouin's
fixative. Tissues fixed in HistoChoice® MB exhibit vibrant staining, better nuclear and
cytoplasmic detail and display a crisp appearance even after long-term fixation. HistoChoice®
MB fixed tissue also retains a much more natural look and feel than formalin fixed tissue.




Non-formalin based tissue fixative for molecular biology applications
Preserves native state antigenic and nucleic acid sites
Tissues retain natural look and feel compared to formalin fixed tissues
A safe, non-toxic fixative- contains no formaldehyde, glutaraldehyde or mercury
Storage/Stability
Store at room temperature (18 to 26°C).
Product Use Limitations
For research use only. Not for therapeutic or diagnostic use.
AMRESCO, LLC Corporate Headquarters, 28600 Fountain Parkway, Solon, OH 44139
Directions for Use
Protocol/Procedure
Note: HistoChoice® MB (Molecular Biology) Tissue Fixative is designed to replace formalin
and may be used in the customer’s standard protocol for formalin fixation with the following
considerations:

Minimum fixation times:
o
Cells, 15-20 minutes
o
Sections on slides, 45 minutes
o
Tissue (1 cubic centimeter), 2-3 hours

HistoChoice® MB is a non-crosslinking fixative. Tissues will be softer and exhibit a more
natural appearance than formalin-fixed tissues. If firmer tissue is desired, use 4 parts
HistoChoice® MB to 1 part ethanol.

Do not predigest tissue (proteinase K, trypsin, etc.)!

In many instances, primary probe or primary antibody concentration may be decreased
by up to 50% without compromising results of in situ hybridization and
immunohistochemistry experiments.

The osmolality of a 1:10 dilution of HistoChoice® MB in deionized water is approximately
340-360 mOsm/kg H2O. Adjustments will need to be made with sodium chloride or
sucrose if working with marine organisms.
AMRESCO, LLC Corporate Headquarters, 28600 Fountain Parkway, Solon, OH 44139
Directions for Use
References
MiR-93 enhances angiogenesis and metastasis by targeting LATS2. Fang L, Du W, Yang W, Rutnam Z,
Peng C, Li H, O'Malley Y, Askeland R, Sugg S, Liu M, Mehta T, Deng Z, Yang B. Cell Cycle. 11, 4352,
(2012).
Immunohistochemical localization of feline immunodeficiency virus using native species antibodies.
Rogers AB, Mathiason CK, Hoover EA. Am J Pathol. 161(4), 1143, (2002).
HistoChoice as an alternative to formalin fixation of undecalcified bone specimens. Kacena MA.
Biotech. Histochem. 79, 185, (2004).
Histomorphologic assessment of formalin substitute fixatives for diagnostic surgical pathology. Titford ME.
Arch. Pathol. Lab. Med. 129, 502, (2005).
The use of Histochoice for histological examination of articular and growth plate cartilages, intervertebral
disc and meniscus. Melrose J. Biotech. Histochem. 83, 47, (2008).
Quantitative comparison of immunohistochemical staining intensity in tissues fixed in formalin and
Histochoice. Vince DG. Anal. Cell. Pathol. 15, 119, (1997).
For Technical Support
Toll Free: 1-800-610-2789 (USA & Canada)
Fax: (440) 349-0235
Email: [email protected]
AMRESCO, LLC
A VWR Company
Corporate Headquarters
28600 Fountain Parkway
Solon, Ohio USA 44139-4300
Tel: 440/349-1199
Fax: 440/349-1182
www.amresco-inc.com
HistoChoice®MB Tissue Fixative
ZY0640
Rev. 1 11/2015
© Copyright 2010 by AMRESCO, LLC
All Rights Reserved.
AMRESCO® is a registered trademark of AMRESCO, LLC
AMRESCO, LLC Corporate Headquarters, 28600 Fountain Parkway, Solon, OH 44139