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Download Directions for Use HistoChoice® MB (Molecular Biology) Tissue
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Directions for Use HistoChoice® MB (Molecular Biology) Tissue Fixative Code Description Size H120-100ML HistoChoice® MB (Molecular Biology) Tissue Fixative 100 mL H120-1L HistoChoice® MB (Molecular Biology) Tissue Fixative 1L H120-4L HistoChoice® MB (Molecular Biology) Tissue Fixative 4L General Information VWR Life Science AMRESCO’s HistoChoice® MB (Molecular Biology) Tissue Fixative is specially formulated to preserve antigenic sites (for antibody probes) and nucleic acid sites (for in situ hybridizations) in their native state, rendering pre-digestion or other recovery procedures for these important sites unnecessary. Primary antibodies can often be diluted several-fold due to the increased number of preserved antigenic sites, saving cost on every slide that is processed. HistoChoice® MB provides superior sensitivity, allowing markers to be observed at lower antibody levels. It makes an excellent transport medium and can be safely washed down the drain upon neutralization in most facilities. HistoChoice® MB directly replaces common fixatives, including formaldehyde-based, alcohol-based, Zenker's, B5 and B3, and Bouin's fixative. Tissues fixed in HistoChoice® MB exhibit vibrant staining, better nuclear and cytoplasmic detail and display a crisp appearance even after long-term fixation. HistoChoice® MB fixed tissue also retains a much more natural look and feel than formalin fixed tissue. Non-formalin based tissue fixative for molecular biology applications Preserves native state antigenic and nucleic acid sites Tissues retain natural look and feel compared to formalin fixed tissues A safe, non-toxic fixative- contains no formaldehyde, glutaraldehyde or mercury Storage/Stability Store at room temperature (18 to 26°C). Product Use Limitations For research use only. Not for therapeutic or diagnostic use. AMRESCO, LLC Corporate Headquarters, 28600 Fountain Parkway, Solon, OH 44139 Directions for Use Protocol/Procedure Note: HistoChoice® MB (Molecular Biology) Tissue Fixative is designed to replace formalin and may be used in the customer’s standard protocol for formalin fixation with the following considerations: Minimum fixation times: o Cells, 15-20 minutes o Sections on slides, 45 minutes o Tissue (1 cubic centimeter), 2-3 hours HistoChoice® MB is a non-crosslinking fixative. Tissues will be softer and exhibit a more natural appearance than formalin-fixed tissues. If firmer tissue is desired, use 4 parts HistoChoice® MB to 1 part ethanol. Do not predigest tissue (proteinase K, trypsin, etc.)! In many instances, primary probe or primary antibody concentration may be decreased by up to 50% without compromising results of in situ hybridization and immunohistochemistry experiments. The osmolality of a 1:10 dilution of HistoChoice® MB in deionized water is approximately 340-360 mOsm/kg H2O. Adjustments will need to be made with sodium chloride or sucrose if working with marine organisms. AMRESCO, LLC Corporate Headquarters, 28600 Fountain Parkway, Solon, OH 44139 Directions for Use References MiR-93 enhances angiogenesis and metastasis by targeting LATS2. Fang L, Du W, Yang W, Rutnam Z, Peng C, Li H, O'Malley Y, Askeland R, Sugg S, Liu M, Mehta T, Deng Z, Yang B. Cell Cycle. 11, 4352, (2012). Immunohistochemical localization of feline immunodeficiency virus using native species antibodies. Rogers AB, Mathiason CK, Hoover EA. Am J Pathol. 161(4), 1143, (2002). HistoChoice as an alternative to formalin fixation of undecalcified bone specimens. Kacena MA. Biotech. Histochem. 79, 185, (2004). Histomorphologic assessment of formalin substitute fixatives for diagnostic surgical pathology. Titford ME. Arch. Pathol. Lab. Med. 129, 502, (2005). The use of Histochoice for histological examination of articular and growth plate cartilages, intervertebral disc and meniscus. Melrose J. Biotech. Histochem. 83, 47, (2008). Quantitative comparison of immunohistochemical staining intensity in tissues fixed in formalin and Histochoice. Vince DG. Anal. Cell. Pathol. 15, 119, (1997). For Technical Support Toll Free: 1-800-610-2789 (USA & Canada) Fax: (440) 349-0235 Email: [email protected] AMRESCO, LLC A VWR Company Corporate Headquarters 28600 Fountain Parkway Solon, Ohio USA 44139-4300 Tel: 440/349-1199 Fax: 440/349-1182 www.amresco-inc.com HistoChoice®MB Tissue Fixative ZY0640 Rev. 1 11/2015 © Copyright 2010 by AMRESCO, LLC All Rights Reserved. AMRESCO® is a registered trademark of AMRESCO, LLC AMRESCO, LLC Corporate Headquarters, 28600 Fountain Parkway, Solon, OH 44139