Download 46 Cell Biology. - Tufts University

Biology 46: Cell Biology
Spring 2017
Dr. Susan Koegel
Office: Barnum 107
Phone: 627-3196
Email: [email protected]
Course Hours: Mondays, Wednesdays 10:30-11:45 (E+), Anderson 112
Office Hours: Tuesdays 11-12:30, Fridays 2-3:30 & by appointment
TA: Rory Fuller ([email protected])
TA Office Hours: Thursdays 9:30-10:30, Barnum 216C
Course Description
The cell is a fascinating, complex, and dynamic unit that forms the fundamental basis of
unicellular and multicellular life. Cells are constantly engaging with their environment
and making active decisions, and we will dissect these cellular processes. Topics
include: signal transduction, membrane dynamics, vesicle transport, cytoskeleton
dynamics, cell migration, cell cycle, stem cells, and mechanisms of gene expression
regulation. We will use the study of disease states, including cancer, to explore these
topics and how alterations can lead to disease. In addition, drug therapies will be
discussed as they highlight an understanding of a cellular process. We will use data
from original research papers to drive our study of this discipline. Throughout this
course, there will be an emphasis on developing hypotheses, designing experiments,
and analyzing data.
Course Objectives
By the end of the semester, you should be able to:
• Appreciate and understand the dynamic nature of the cell, including how it
receives and responds to information from its environment.
• Explain and compare different mechanisms for receptor activation and regulation.
• Understand and explain how membrane chemistry and regulation are essential in
cell communication.
• Understand intracellular signaling cascades and their impact on cellular activities,
including cytoskeleton rearrangements, motility and changes in gene expression.
• Understand mechanisms of cell cycle regulation.
• Predict how alterations or given drugs/chemical treatments would impact cellular
behavior.
• Develop testable hypotheses based on provided information, design experiments
to test hypotheses, and interpret provided data.
Preparing for Class
Biology 46 Cell Biology/Spring 2017/Page 1
It is expected that you complete assigned readings and review any posted
sources before attending class.
The required textbook is:
Alberts et al. Molecular Biology of the Cell, 6th edition.
Two copies of the 6th edition will be kept on three-hour reserve in the library.
Additional articles on the reading list will be made available on the course’s Trunk site.
To help you focus your reading, Guided Reading Questions will be posted under
Class Materials on Trunk.
Course Requirements
Grading:
Exams: 70% (two highest exams worth 25% each, lowest worth 20%)
Group Poster Presentation: 20% (90% Dr. Koegel’s evaluation, 10% peerevaluation)
Participation: 10% (learning catalytics questions)
Please note that there will be no extra credit opportunities in this course.
Exams: You will have two in-class exams and a third one hour exam during finals
period. If you are requesting an accommodation for a documented disability, you must
register with the Accessibility Services Office at the beginning of the semester. To do so,
call the Student Services Desk at 617-627-2000 to arrange an appointment.
Group Poster Presentation: You will prepare a presentation and scientific poster that
will focus on the identification and evaluation of a drug target to treat a disease of
interest. This group presentation will count as 20% of your final grade. All members of
the group will receive the same grade.
In-Class Questions: You are expected to actively participate in this course. You will
learn by engaging with the material and your peers; therefore, your participation in this
course is extremely important for everyone’s learning.
We will be using Learning Catalytics, an automated classroom response system to
provide an opportunity for you to answer questions in class using a web-enabled device.
Your participation in these exercises (not whether or not you get the correct answer) will
contribute towards your final grade. You are required to complete 90% of the questions
from February 1st to the end of the semester to receive the full 10% credit (given that
you only need to answer 90% of the questions, credit will NOT be given for absence
due to illness or personal reasons). You cannot make-up missed questions. Learning
Catalytics will be used in every class, and you are responsible for bringing your device
to each class. To sign up for an account, please follow the directions posted on
Trunk/Announcements.
Biology 46 Cell Biology/Spring 2017/Page 2
I expect you to engage in in-class discussions and offer ideas and questions. Even if
you are unlikely to talk in a large discussion, smaller group discussions will provide
another avenue for participation. Failure to actively participate will negatively affect your
participation grade.
Class Policies
Posting Lectures: I will post slides before each lecture on our course Trunk site that I
encourage you to print and bring to class. Slides with in-class annotations will be
posted within 24 hours after class.
Email Policy: I will try and respond to all emails within 24 hours of receiving them. This
time frame is not guaranteed, especially over weekends or holidays.
Academic Honesty
You will work collaboratively throughout the course to solve problems and work on your
final presentations. Your work on exams should be exclusively your own. Cheating and
plagiarism will not be tolerated and will be reported to the university. You must correctly
cite your sources on your final project (instructions on how to do so will be provided).
Important Dates
• February 8th: Poster presentation group assignments due
• February 15th: Exam 1
• February 23rd: Group poster presentation topic due
• March 15th: Exam 2
• March 29th: References due
• April 10th: Poster Summary Due
• April 24th, 27th: Group poster presentations
• May 8th: Exam 3
Biology 46 Cell Biology/Spring 2017/Page 3
Class Schedule
(Subject to Modification)
Topic
Jan. 23rd
Introduction to Cell Signaling and the
Hallmarks of Cancer
Jan. 25th
Oncogenes and Tumor Suppressors
1091-1096, 11041105, 155-156,
813-817
1
Jan. 30th
Receptor Tyrosine Kinases
850-853, 822-823,
452-455, 489, 487
2
Feb. 1st
G-protein Coupled Receptors
820, 832-836, 848849
Feb. 6th
Membrane Transport
597-614
Feb. 8th
Ion Transport and Calcium
Poster presentation group assignments due
546-547, 655 (Fig
12-15), 836-841
Feb. 13th
Vesicle Transport and Endocytosis
695-703, 730-736
Feb. 15th
Exam 1 (on material through Feb. 8th)
Feb. 20th
No Classes (Presidents’ Day)
Feb. 22nd
Familial Hypercholesterolemia & Receptor
Mediated Endocytosis
Feb. 23
rd
Feb. 27th
Textbook
Additional
Materials
Date
3
4, 5*
Tufts Monday
Ras/MAPK Pathways and the Targeting of
Intracellular Signaling Pathways
Group poster presentation topic due
543-546, 820, 854857, 1106, 11391140
Actin Cytoskeleton Organization and Cell
Migration
889-895, 898-917,
951-960
Biology 46 Cell Biology/Spring 2017/Page 4
March 1st
Cell Migration & Metastasis
March 6th
Cell-Cell Interactions and Extracellular Matrix
1035-1042, 10541081
March 8th
Targeting Angiogenesis
1120, 1235-1238
March 13th
Regulation of Gene Expression by Non-Coding
429-433, 499-501
mRNAs
March 15th
Exam 2 (on material through March 8th)
6, 7*
Spring Break: March 20th – March 24th
March 27th
Discovering Cell Cycle Regulation
488-489, 963-970,
978-979, 980-981,
1012-1013
March 29th
The Spindle Assembly Checkpoint
References due
925-931, 971, 982995
April 3rd
p53 and Apoptosis
1014-1017, 10211032
April 5th
The Roles of Ubiquitin
157-158, 357-360
April 10th
Autophagy
Poster Summary Due
722-729
10
April 12th
Stem Cells
868-871, 12171224, 1251-1260
11
April 17th
No Classes: Patriot’s Day
April 19th
Stem Cells & Cancer
April 24th
Targeted Therapy: Poster Presentations Day 1
8, 9
12
Biology 46 Cell Biology/Spring 2017/Page 5
April 26th
Targeted Therapy: Poster Presentations Day 2
May 1st
Bringing it all Together: Cells Inside and Out
May 8th
Exam 3 – 3:30-5:30
Biology 46 Cell Biology/Spring 2017/Page 6
Non-Textbook Required Reading List (Available on Trunk Under Class Materials)
Additional articles may be added during the course of the semester (* denotes primary
research article)
1
2
3
4
5
6
7
8
9
10
11
12
Borzac K. (2014) Three known unknowns. Nature 509: S69-S71.
Oxnard G. (2016) The origins of drug resistance in cancer. Nature Medicine 22: 232234.
Chandy KG and Norton RS. (2016) Channeling potassium to fight cancer. Nature 537:
497-499.
Nair P. (2013) Brown and Goldstein: The Cholesterol Chronicles. PNAS 110: 1482914832.
Brown and Goldstein. (1974) Familial Hypercholesterolemia: Defective binding of
lipoproteins to cultured fibroblasts associated with impaired regulation of 3-hydroxy-3methylglutaryl coenzyme A reductase activity. PNAS 71: 788-792.
Farrell A. (2006) Bloodlines. Nature Milestones Cancer. doi:10.1038/nrc1849
Kim KJ et al. (1993) Inhibition of vascular endothelial growth factor-induced
angiogenesis suppresses tumour growth in vivo. Nature 362: 841-844.
McCarthy N. (2006) Death Defying. Nature Milestones Cancer. doi:10.1038/nrc1854
Vogelstein B. et al (2010) p53: The most frequently altered gene in human cancers.
Nature Education 3:6.
Castro-Obregon S. (2010) The discovery of lysosomes and autophagy. Nature
Education 3: 49.
Suomalainen A. (2015) Asymmetric rejuvenation. Nature 521: 296-297.
Couzin-Frankel J. (2015) The bad luck of cancer. Science 347: 12.
Biology 46 Cell Biology/Spring 2017/Page 7