Download Optimizing unnatural amino acid mutagenesis in mammalian cells

Optimizing unnatural amino acid mutagenesis in mammalian cells Unnatural amino acid mutagenesis, also called amber suppression is a promising technique to control and study protein function in living cells. It relies on expanding the standard genetic code by recoding the amber stop codon to incorporate an unnatural amino acid. We are striving to develop this technique into a robust and general tool for mammalian cell biology, in particular chromatin and stem cell biology. The master project will involve developing and translating an original idea into a series of well­controlled experiments in mammalian cells. We are looking for a motivated student with knowledge and ideally experience in cell culture and molecular biology techniques. Contact [email protected] Tel 0852481227 www.elsaesserlab.org Further Reading Schmied WH*, ​
Elsässer SJ*, ​
Uttamapinant C, Chin JW, „Optimized Pyrrolysyl tRNA synthetase/tRNA expression and engineered eRF1 enable efficient multi­site unnatural amino acid incorporation in mammalian cells“, JACS, (2014) Elliot T, Townsley F, Bianco A, Ernst R, Sachdeva A, ​
Elsässer SJ​
, ..., Chin JW, „Proteome labeling and protein identification in specific tissues and at specific developmental stages in an animal“, Nature Biotechnology (2014) Nguyen D, Mohan M, ​
Elsässer SJ​
, Uttamapinant C, Chin JW, „Genetic encoding of photocaged cysteine allows photoactivation of TEV protease in live mammalian cells“, JACS (2014)