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Download Protein Expression and Purification Service Inquiry Form
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SB Sino Biological Inc. Biological Solution Specialist Protein Expression and Purification Service Inquiry Form This form is aimed to know accurate info about clients and their requirements, so that we can provide faster quotation and better services. Please fill the form and send it to [email protected]. We will reply to you within 1-2 business days upon receipt of this form. Thanks for your cooperation. 1. Contact Information Date of Request Client Name Phone Organization Name Shipping Address Email 2. Protein Information Protein Name Protein Sequence Sequence info Sub-cellular Location Protein Property Accession# Species The protein sequence is a native one. The protein sequence has been modified. If yes, please specify the modified site. Cytoplasmic Nuclear Membrane Secreted Other (please specify) Enzyme Protease Toxic Prone to aggregation Prone to degradation Insoluble Other (please specify) Molecular Weight (kD) Expression Level Please indicate crude or post-purification (mg/L) Previously If yes, please attach growth and purification protocols Produced/Purified? Suite B-209 14 Zhong He Street, BDA Beijing 100176, P.R. China Fax. 86-10-5102-9969 E-mail: [email protected] SB Sino Biological Inc. Biological Solution Specialist 3. Expression and Purification Requirement Starting Materials Expression System Affinity Tag Affinity Tag Terminus Affinity Tag Removal after purification Required Amount (mg) Required Purity (%) Sequences, gene synthesis is needed Your template DNA/cDNA, sub-cloning is needed (please provide plasmid info, eg. plasmid name, antibiotic resistance, restriction enzymes and fragments, plasmid map if available, etc.) Plasmid that Sino already has Stable cell line for protein production (please also provide cell line info, yield and culture conditions) HEK293 transient CHO transient HKE293 stable CHO stable Insect-Baculovirus E.coli Yeast HIS GST Fc Flag AVI SUMO Other (please specify) N-terminus C-terminus Remove Do not remove Detected by SDS-PAGE: (please specify) Detected by SEC: (please specify) Required Concentration (mg/ml) Special Requirement Final Formulation Date Wanted By Date Needed By Intended use Shipping Other Comments Suite B-209 14 Zhong He Street, BDA Beijing 100176, P.R. China Endotoxin level (please provide required content EU/mg) Others (please specify) Standard PBS, pH7.4 Storage buffer Other (please specify) Delivery Size: (protein quantity per vial) e.g. date of study initiation Activity study Structural Study Antigen Drug Screening Cell based experiment In vivo experiment In vitro experiment Other (please specify) 2-8℃ -20℃ frozen gel packs -80℃ dry ice Please specify if there is any. If you need to express several proteins with the same quality, please indicate in this section. Fax. 86-10-5102-9969 E-mail: [email protected]
