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Goal to Excite Confidence, Curiosity, Creativity and Perseverance SCHEDULE ANNOUNCED (March – May 2016) Dates Trainings 25 March 2016 *DNA Isolation Contents Strategies for DNA Isolation Samples Preparation (2) Reagents Preparation DNA Extractions DNA Quantification Agarose Gel Electrophoresis (AGE) Analysis of Bands Troubleshooting No. Registration Fee (RS) of Days Regular: 6000 1 Student UAF: 3600 Employee UAF: 4200 Other Govt. Institutions: 4800 26 March 2016 7-9 April 2016 *PCR Gene Cloning 21 April 2016 *Protein Isolation 22-23 April 2016 *SDS-PAGE Overview of PCR Introduction about Primer Designing Reaction Preparation Thermocycler Instrumentation PCR Running Agarose Gel Electrophoresis (AGE) Analysis of Bands Troubleshooting Basic Concepts of Recombinant DNA Technology Culture Preparations Preparation of Competent Cells Ligation of Gene into Vector Transformation of Vector into Host Cloning Strategies Blue White Screening Interpretation of Colonies Plasmid Miniprep Troubleshooting Guide to Protein Extraction Samples Preparation (2) Reagents Preparation Extraction of Proteins Quantification of Proteins Troubleshooting Principle of SDS-PAGE Reagents Preparation Gel Castings 1 Regular: 6000 Student UAF: 3600 Employee UAF: 4200 Other Govt. Institutions: 4800 3 Regular: 15000 Student UAF: 9000 Employee UAF: 10500 Other Govt. Institutions: 12000 1 Regular: 6000 Student UAF: 3600 Employee UAF: 4200 Other Govt. Institutions: 4800 2 Regular: 10000 Student UAF: 6000 Employee UAF: 7000 Other Govt. Institutions: 8000 6-7 May 2016 DNA Fingerprinting 20 May 2016 *RNA Isolation 21 May 2016 *RT-PCR Sample Preparation, Loading and Running Staining of Gels Protein bands analysis Results Interpretation Gel Preservation Excision of Bands Troubleshooting Idea behind DNA Finger Printing RAPD DNA Extraction (2) Polymerase Chain Reaction (PCR) Agarose Gel Electrophoresis (AGE) RAPD Analysis Results Interpretation Troubleshooting Strategies for RNA Isolation Samples Preparation (2) Reagents Preparation RNA Extraction RNA Quantification Agarose Gel Electrophoresis (AGE) Analysis of Bands Troubleshooting Overview of RT-PCR Primers of RT-PCR cDNA Synthesis Reaction Preparation Thermocycler Instrumentation RT-PCR Running 2 Regular: 7000 Student UAF: 4200 Employee UAF: 4900 Other Govt. Institutions: 5600 1 Regular: 6000 Student UAF: 3600 Employee UAF: 4200 Other Govt. Institutions: 4800 1 Regular: 6000 Student UAF: 3600 Employee UAF: 4200 Other Govt. Institutions: 4800 28 May 2016 Bioinformatics (Basics) Agarose Gel Electrophoresis (AGE) Results Interpretation Troubleshooting Introduction to Bioinformatics Biological Databases Sequence Analysis Sequence Alignment Primer Designing Genomics Proteomics Phylogenetic Analysis 2 Regular: 6000 Student UAF: 3600 Employee UAF: 4200 Other Govt. Institutions: 4800 Note: 4-8 participants/ Training except Bioinformatics (Basics), 6-20 participants. If you are interested, register immediately to confirm your spot. For Registration: Download Proforma from www.molcare.org and send at [email protected] *Special Offers are also available. Please contact Manager Trainings. Nazia Kanwal Manager Trainings PhD scholar Molecular Care Department of Biochemistry, UAF Email: [email protected] Mob: 0333-1929859 For further details and disclaimers: www.molcare.org