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Dear Reviewer #2
Thank you for the thoughtful and thorough review. Our responses to your
comments are as follow:
producing plant via a mikimopine producing
Response: This statement was changed as follows:
hairy roots were established in different explants of Linum mucronatum, an anticancer agent producing plant, via a mikimopine type strain of Agrobacterium
rhizogenes, ‘A13’.
o
Response: degree sign was changed to: °C
Agrobacterium rhizogenes, hairy root, Linum mucronatum, MS-based liquid medium
Response: the keywords were changed as follows:
Agrobacterium rhizogenes, explant, growth medium, medicinal plants, secondary
metabolites
Meanwhile, the title (a long title) of the manuscript was changed to:
“ Hairy Root Induction in Linum mucronatum ssp. mucronatum, an Anti-Tumor
Lignans Producing Plant ”
Iankova
Response: “Iankova” was changed to “ Ionkova ”
Mukundan et al., 1998; Christensen and Muller, 2009
Response: was arranged in alphabetical order:
(Christensen and Muller, 2009; Mukundan et al., 1998)
2000
Response: was changed to “Kim et al., 2002”
Oostdam et al., 1993; Lin et al., 2003
Response: was arranged in alphabetical order: (Lin et al., 2003; Oostdam et al 1993).
To our knowledge, there are no
Response: The statement was changed as follows:
Based on the scientific literature currently available,
°C
Response: The sign was changed to: °C
kindly provided by Dr. MA Malboobi,
Response: person name was deleted.
Luria-Bertani
Response: cultured into LB liquid medium (Bertani., 1952)
with some modifications
Response: the statement was deleted because the modification was very slight.
PCR
Response: the full name was inserted: Polymerase chain reaction analysis
o
Response: The sign was corrected in the all parts of the text
SAS Institute Inc., Cary, NC, USA, Release 9.1),
Response: (SAS Institute Inc., 2004)
Fisher’s
Response: the related reference was inserted in the text and added to Reference
chapter (Fisher, 1954)
growth
Response: was corrected to:
development
Whereas, there were no hairy roots formed from control explants. When cotyledon
explants were also infected and co-cultured with Agrobacterium, no hairy roots
induced on these explants.
Response: the statement was changed to:
, whereas no adventitious roots formed from the control explants. Also,
Agrobacterium-inoculated cotyledon explants did not show induction of roots (data
not shown).
Our results indicated that
Response: the statement was changed to:
The results indicated that…
, our results
Changed to: the results of the present study…
LBA9402
Response: apostrophe mark was used for any strain name in whole document, such
as: ‘LBA9402̕ and ‘TR105̕.
Red arrows
Response: Black arrows
Handa, 1992; Godo et al., 1997; Ohara et al., 2000, Ishizaki et al., 2002, Koike et al.,
2003, Akutsu et al., 2004,
Response: was arranged in alphabetical order:
(Akutsu et al., 2004; Fukuda et al., 2007; Godo et al., 1997; Handa, 1992; Ishizaki et
al., 2002; Koike et al., 2003, Ohara et al., 2000)
Fukuta
Response: was corrected to: Fukuda
Yoshikawa and Furuya, 1987; Sato et al., 1991; Nilsson and Olsson, 1997)
Response: was arranged in alphabetical order:
(Nilsson and Olsson, 1997; Sato et al., 1991; Yoshikawa and Furuya, 1987)
et al
Response: was corrected to: et al
Medium composition as follow: HRGM-A: Basal MS Medium with 3% Sucrose,
HRGM-B: Basal MS Medium with 3% Sucrose supplemented with 0.2 mg/l NAA.
HRGM-C: Basal MS Medium with 2% Sucrose, HRGM-D: the same as HRGM-A
with incubation temperature of 35oC.
Response: was changed to:
Effects of various culture media on the growth of transgenic hairy root lines produced
by A. rhizogenes strain ‘A13’ in L. mucronatum. The bars represent means ± SE. Bars
followed by different letters are significantly different (P < 0.05) according to FLSD.
L-1
Response: Yes and it was corrected.
T-DNA was transformed into hairy roots of L. mucronatum by A. rhizogenes
Response: the sub-chapter was changed to:
Detection of relevant transgenes in the selected hairy root lines
Successful genetic transformation can be demonstrated in two ways. Directly or
indirectly, by detecting T- DNA sequences or opines respectively.
Response: the statement was corrected to:
Successful genetic transformation can be demonstrated in direct or indirect ways by
detecting opines or T-DNA sequences, respectively.
To detect T-DNA, polymerase chain reaction (PCR) can be used simply
Response: the statement was changed to:
PCR method can be used simply to detecting T-DNA sequences in putartive
transformants.
our
Response: it was changed to :
In this study,
no detectable amplicon was observed
Response: the statement was changed to:
, but no such amplicon was observed in the untransformed root (negative control)
sample.
6:
Response: is inserted 6 number in figure.
Also,
Response: The statement was changed to:
They also formed a callus in phytohormone-free medium
Fukuta
Response: was corrected to Fukuda.
(Ohara et al., 2000; Ishizaki et al., 2002; Akutsu et al., 2004, Fukuta et al., 2007
Response: was arranged in alphabetical order:
(Akutsu et al., 2004; Fukuda et al., 2007; Ishizaki et al., 2002, Ohara et al., 2000)
We
Response: the statement was was changed to:
The present study reports the successful production of transgenic hairy root lines in L.
mucronatum.
2002
Response: The correct year is 2002 that was corrected.
The authors wish to thank you again for your constructive comments and
hopefully we have addressed all of your concerns.
Best Regards,
Corresponding Author