Download LUMIprobe24 Technique Enrichment included LISTERIA SPECIES

LUMIPROBE24
Technique
Enrichment
included
LISTERIA SPECIES,
MONOCYTOGENES
SALMONELLA SPECIES
MEDIA
ENRICHMENT BROTH RM (Rich Medium)
Advantages:
RM is a key component of our technique sensitivity.
 Thanks to its original formulae, it allows a fast revival of stressed microorganisms
and their accelerated growth for a shorter enrichment phase.
 For Listeria , the RM ensures in 22 h and only one step( except for dairy products)
the presence of enough bacteria for their detection.
 For the particular case of detection of Listeria in milk and dairy products, a specific
broth has been developed: RML, taking in account the background flora, and the
enrichment is run in 2 steps.
 For the detection of Salmonella, RM is used for the enrichment step, before the
selective one.
 So RM is common in the case of an associated detection of Listeria and
Salmonella on the same sample and it allows only one weight.
 Time and money savings
SELECTIVE BROTH RV
Advantages:
RV broth allows a selective enrichment for Salmonella, following the first step in RM .
RM, RML and RV media are available in 3 items:
 Ready to use broths : 225 mL bottle or 2.5 L flask
 Powder
: 500g
LUMIPROBE 24 DETECTION KITS
2 types of kits are available:
 Tubes for small and medium serials: kit of 50 tests
 Microplate: 12 strips of 8 wells - kit of 96 tests
 the 4 reagents are ready to use
 the washing buffer is 20x concentrated
 tubes or microplate are packed in aluminium zipped pouch.
Available :
 Detection of Salmonella sp.
 Listeria sp, Listeria monocytogenes
LUMIPROBE 24 Equpment
TESTS IN TUBES : RUN < 30 TESTS
LumLite
or
LumLite Mini
LUMINESCENCE DETECTION SYSTEMS LumLite:
RELIABLE:
 High Sensitivity
 Easy to run. Pre defined protocols.
 Traceability of results
OPEN:
 To all luminescence applications
 Dispenser (option)
Chimiluminescence tests :
Example : DNA Probe hybridization Techniques as LUMIPROBE 24
Bioluminescence tests : Ex ATP métrie
Principle : Quantification of photons into RLU or Relative Light Units
Sensitivity: Dйtection < 1000 luciferase molecules
Reading scale : 5 to 6 magnitude levels
Measuring time: 0 /99.9 secondes
Interface : RS 232
LumLite technical specifications:
Compact equipment : bench or portable (2.5 kgs-L x l x h = 22 x 17 x 22 cm)
Accepts all kind of sample (loaded in " reading chamber")
Liquid crystals display and 3 command keys
In house microprocessor driving the luminometer and the associated printer.
LUMINESCENCE DETECTION SYSTEMS PhL:
MICROPLATE TESTS: RUN > 30 TESTS
MODULAR CHAIN AutoLite including :
 Incubator/Shaker,
 Programmable washer,
 PhL / Photometer / Luminometer / Injectors / Shaker /
Incubator
 5 functions associated in a compact equipment, for a
maximum number of applications
PhL technical specifications:
Polyvalent microplate reader
Photometer:
2 wavelengths readings:
Choice among 13 filters 340 а 690 nm
Sensitivity < 0.002 DO
Reading scale: > 4.5 DO
Luminometer: Detector: High sensitivity Hamamatsu Photomultiplier
:
Sensitivity: 20 amol ATP
Reading scale: 7.5 decades
Applications : Food analysis, environment, sanitation controls,
biotechnology : ATP measurement, luciferine-luciferase, RNA or DNA
labelled probes, phagocytose, immunology, enzymology
Injectors:
Reagents addition:
 Automated for " flash " luminescence
 Accurate: < 1 %
 Choice of volumes: 0 to 300 µL by steps of 10µL
Shaker:
For better mixing and shorter analysis time
Software:
Friendly access to procedures:
 Reagents dispensing
 End point, kinetic, integration, measurements
 Data analysis, printing and storage of results
 Memory for routine procedures.