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Detection of Potato Leaf Roll Virus (PLRV), Potato Virus Y (PVY) and Potato Virus X
(PVX) on Five Potato Varieties by Using of DAS-ELISA and RT-PCR Methods
TUTIK KUSWINANTI, BAHARUDDIN, GUSMIATI AND MASIRA. S
Department of Plant Pests and Diseases, Faculty of Agriculture, Hasanuddin University,
Jl. Perintis Kemerdekaan KM. 10., Makassar 90245
E-mail : [email protected]
Potato is a staple food crop that widely grown around the world. Virus infection is main
factor that affects great loss of the potato production. Potato virus X(PVX), potato virus
Y(PVY),and potato leaf roll virus(PLRV) are top three viruses that result in decreased yield of
potato in Indonesia. Therefore, the rapid methods of DAS-ELISA was studied to test tuber
samples of five potato varieties, Granola, Atlantik, Raja, Super John, Kalosi, and Masalle. Two
simple, rapid, sensitive, reliable detection techniques of three virus are reported in the paper:
they are DAS-ELISA and RT-PCR. Detection of all three viruses on positive control samples
could be carried out with ELISA technique without purification of RNA virus. Neither PLRV
nor PVX were detected on five potato varieties test samples, but PVY was detected only in
Kalosi test sample. This method is simpler and rapider than RT-PCR technique, and is more
suitable for the detection of a great number of potato samples. The sensitivity of ELISA is as
same as that of RT-PCR.
Keywords : PLRV, PVY, PVX, DAS-ELISA, RT-PCR