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Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > ASMC Transfection Reagent (Aortic Smooth Muscle Cells)
Altogen Biosystems offers the ASMC Transfection Reagent
among a host of 100+ cell line specific In Vitro Transfection
Kits.
ASMC Transfection Reagent is an advanced formulation of a
lipid based reagent, and it has been developed to provide high
transfection efficiency with ASMC and other vascular cell lines.
This cell line is a good host for studying human vascular
disease. When cultured in vitro, ASMC cells are a monolayer
and adhere to the surface of the culture flask.
Other applications include drug discovery, gene expression
studies, molecular and cell biology research applications.
Purchase ASMC Transfection Kit at www.Altogen.com
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > ASMC Transfection Reagent (Aortic Smooth Muscle Cells)
ASMC Cell Line Description and Applications
High Density ASMC Cell Line
atcc.org
The ASMC or Aortic Smooth Muscle Cells originate from
the human tunica intima and tunica media of healthy aorta
tissue. The tunica media is made up of mostly smooth
muscle cells as well as elastic fibers that are arranged in
spirals; it is the middle layer of the artery. The tunica intima
is the inner layer of the artery and it is made up of a layer of
elastic fibers and a network of connective tissue. ASMC
cells can synthesize glycosaminoglycan, myosin, collagen
and elastin and can also differentiate in response to various
factors. The ASMC cells can be used to study
atherosclerosis, angiogenesis, stroke, diabetes, vascular
biology, diabetes and other human vascular disorders.
ASMC cells are sensitive to IGF1 and IGF2, but resistant to
insulin at the receptor level. Via the inhibition of MMP-9
expression, curcumin can prevent migration of ASMC cells.
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > ASMC Transfection Reagent (Aortic Smooth Muscle Cells)
ASMC Transfection Protocol
1. Plate 10,000 - 15,000 ASMC cells per well in 0.5 ml of complete growth medium 12–24 hours prior to
transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for 15 - 30 minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore
increasing transfection efficiency; however it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with ASMC cells (from step 2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression 48 - 72 hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of
Transfection Enhancer reagent 12-24 hours after transfection
If the viability of ASMC cells being transfected is affected at 16 - 24 hours post-transfection, the level of cytotoxicity
can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > ASMC Transfection Reagent (Aortic Smooth Muscle Cells)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Products > ASMC Transfection Reagent (Aortic Smooth Muscle Cells)
ASMC Transfection Kit Product Details
•
Two component formulation enhances lipid mediated transfection efficiency
•
Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA,
mRNA, and microRNA
•
Kit includes Transfection Enhancer reagent and recommended transfection protocol
•
High transfection efficacy in the presence of serum
•
Expand your RNAi application with a reagent optimized for delivery of both siRNA and
plasmid
•
Reproducible transfection results
•
Works well for standard reverse transfection and high-throughput applications
Developed and manufactured by Altogen Biosystems (www.Altogen.com)
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > ASMC Transfection Reagent (Aortic Smooth Muscle Cells)
Data
Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected following
the recommended protocol. At 48 hours post-transfection the ASMC cells were analyzed by qRT-PCR for
gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are
normalized to untreated sample. Data are means ± SD (n=3).
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > ASMC Transfection Reagent (Aortic Smooth Muscle Cells)
Data
Figure 2. Protein expression of GAPDH in ASMC cells. DNA plasmid expressing GAPDH or siRNA targeting
GAPDH were transfected into ASMC cells following Altogen Biosystems transfection protocol. At 72 hours
post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total
protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > ASMC Transfection Reagent (Aortic Smooth Muscle Cells)
ASMC Transfection Kit Benefits
• Pre-optimized transfection protocol for ASMC cell line
• Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
• Free of serum and protein of animal origin
• Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
• Easy to use ASMC transfection protocol ensures great performance with expedited experimental timeline
• Equally efficient for single or multiple transfections
• Can be used for transient transfection and development of stable ASMC cell lines
• Bio-degradable after endocytosis
• Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]