Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Anatomy 1 Lab Manual, Spring 2009 Anatomy Laboratory Manual Dr. Christina G. von der Ohe Spring 2009 Santa Monica College 1 Anatomy 1 Lab Manual, Spring 2009 2 Table of Contents Course Material Page Syllabus ……………………………………………………………………………………………………… 3 Calendar …………………………………………………………………………………………………… 6 Lab 1: Introduction to Anatomy ………………………………………………………………. 8 Lab 2: Cell Biology ……………………………………………………………………………………. 11 Lab 3: Tissues …………………………………………………………………………………………… 15 Lab 4: Integumentary System ………………………………………………………………….. 17 Lab 5: Introduction to Skeletal System ……………………………………………………. 20 Lab 6: Axial Skeleton ………………………………………………………………………………… 23 Lab 7: Appendicular Skeleton …………………………………………………………………… 26 Lab 8: Articulations …………………………………………………………………………………. 29 Lab 9: Introduction to Muscular System …………………………………………………… 32 Lab 10: Axial Muscles ………………………………………………………………………………. 34 Lab 11: Appendicular Muscles …………………………………………………………………. 36 Lab 12: Cat Dissection ……………………………………………………………………………… 43 Lab 13: Introduction to Nervous System …………………………………………………. 45 Lab 14: Brain ……………………………………………………………………………………………. 48 Lab 15: Spinal Cord and PNS ……………………………………………………………………. 52 Lab 16: Autonomic Nervous System …………………………………………………………. 55 Lab 17: General and Special Senses ………………………………………………………… 57 Lab 18: Endocrine System ………………………………………………………………………… 60 Lab 19: Blood and Heart …………………………………………………………………………… 64 Lab 20: Blood Vessels …………………….………………………………………………………… 67 Lab 21: Lymphatic System ………………………………………………………………………. 70 Lab 22: Respiratory System …………………………………………………………………….. 73 Lab 23: Digestive System …………………………………………………………………………. 76 Lab 24: Urinary System ……………………………………………………………………………. 79 Lab 25: Reproductive System …………………………………………………………………… 82 Anatomy 1 Lab Manual, Spring 2009 3 Anatomy 1: Human Anatomy Instructor: Christina G. von der Ohe, PhD, Professor, Dept. of Life Sciences Office: SC-261 Phone: (310) 434-4662 Email: [email protected] Office hrs: MW 2:30-4:00; Tu 11:00-12:00 SC-261 Meeting: Lecture TTh 7:45-10:50 SC-224 Student Learning Objectives: 1. Name the systems of the human body, their general functions, the major organs that make up these systems, and the general contribution each organ makes to the system. 2. Identify microscopically and describe the structure and basic function of the tissue and cell types used to make up the major organs of the human body. Required Textbooks: Human Anatomy, F. Martini, M. Timmons, B. Tallitsch, 6th ed. Anatomy Laboratory Manual, C. von der Ohe Required Materials: 5 scantrons #882E and a #2 pencil 4 quiz scantrons Dissection kit and disposable gloves Recommended: colored pens and a protective garment Resources: Learning Resource Center and Computer Lab Cayton Center Student Computer Lab Textbook resources at http://www.aw-bc.com/applace Attendance: Roll will be taken at the START of every session. Participation points will be awarded only to students who are on time to class and stay until class is dismissed. Students who are absent for two consecutive meetings or to the first exam without informing the professor with a valid excuse will be dropped from the roster. Drop Dates: Drop dates are listed in your catalog. You are responsible for your enrollment status and the dates and deadlines on the SMC admissions website and schedule of classes. Make-ups: There will be no make-ups for in-class assignments. Only one exam can be made up under extreme circumstances and with instructor consent BEFORE the start of the exam. The make-up exam must be completed before the next exam. The lab practical make-up will be based on digital photos of the in-class practical. The final exam cannot be made up. Anatomy 1 Lab Manual, Spring 2009 4 Course Layout: We will start with an extra credit opening question. Then there will be a lecture. The PowerPoint slides will be posted to ecompanion before the lecture. You are welcome to print the slides before class and take notes on them. After a 20-minute break, we will resume with the laboratory component of the class. Bring your lab manual and your text to every class session. You are required to read the lab manual before coming to class. The lab is based on stations, through which your group will rotate. There will be a mix of traditional learning stations (identification and worksheets) and creative learning stations (drawing, games). At the end of the lab, everyone will pitch in to clean up the lab for the next class. When the lab is clean, I will dismiss the class. Early exits will result in loss of participation points. Opening Question: At the start of every class you will answer a brief extra credit question based on the previous lecture or lab. These questions will help you understand the material in a broader context and will provide feedback about your progress. They should be your own work. We will discuss answers after they are handed in. TBA Hour: You are required to spend an hour per week in the Learning Resource Center (LRC) in the science building, second floor. For hours and dates see schedule on their front door. At the LRC you will find supplemental materials for you to study from. Grading: You will be evaluated based on performance on exams, lab practicals, dissection, and participation. Points will be totaled and expressed as a percent. All grades are non-negotiable and must be earned. 4 lecture exams 400 90-100% = A 1 final exam 200 80-89% = B 4 lab practical exams 200 70-79% = C Quizes 80 60-69% = D Dissection 20 Below 60% = F Attendance and participation 50 TOTAL POINTS 950 Anatomy 1 Lab Manual, Spring 2009 5 To succeed in this class: Anatomy 1 is a very rigorous class that requires considerable discipline, time, and dedication. Tips for success: 1. Leave for class with time to find parking or catch the bus. 2. Be well rested and alert for class. 3. Be prepared for exams. 4. Keep track of your grades on gradebook. 5. Practice effective study habits: - study 30 min to 1 hour every day - study lecture notes soon after lecture - recite the material and draw structures from memory - make sure to engage in class Class Environment: I strive to make the classroom a safe and encouraging learning environment for everyone. There will be a lot of class discussion and group work. Please be respectful of each other. I encourage you to freely ask questions so that everyone can benefit from the discussion. This class is for you. Please turn off all beepers and cell phones during class. Food, drink, and gum are not permitted. I value: 1. 2. 3. 4. 5. 6. Academic Dishonesty: Each student is expected to do his/her own work on all opening questions, lecture exams, and lab practicals. A first offense of academic dishonesty will result in a zero grade on that material. A report will be filed with the Dean of Students with your name and a detailed description of the incident. A second offense anywhere in the college or an especially egregious offense will result in disciplinary action by the professor or the Dean, which can include failing the course, suspension, or dismissal from the college. Please refer to the SMC policy on academic dishonesty posted in the classroom, or refer to the SMC Student Guide. Final Word: If you have any questions about course material, computer, internet, campus resources, future plans, or anything else, please don’t hesitate to ask. I am here to help you. Interest in the material Hard work Respect for everyone in the classroom Integrity in your work Responsibility for your grade Punctuality Anatomy 1 Lab Manual, Spring 2009 6 DATE TOPIC READING (Ch) MANUAL (Ch) Feb 17 Introduction 1 1 Feb 19 Cells 2 2 Feb 24 Tissues 3 3 Feb 26 Quiz, Integumentary System 4 4 Mar 3 Intro to Skeletal System 5 5 Mar 5 Axial Skeleton 6 6 Mar 10 Lecture Exam and Practical 1 Mar 12 Appendicular Skeleton 7 7 Mar 17 Articulations 8 8 Mar 19 NO CLASS Mar 24 Quiz, Intro to Muscular System 9 9 Mar 26 Axial Muscles, Dissection 10 10, 12 Mar 31 Appendicular Muscles, Dissect 11 11, 12 Apr 2 Lecture Exam and Practical 2 Apr 7 Intro to Nervous System 13 & pg362-6, 389-94 13 Apr 9 Brain 15 & pgs443-8 14 Apr 14 SPRING BREAK Apr 16 SPRING BREAK Apr 21 Spinal Cord and PNS 14 & pgs432-42 15 Apr 23 Quiz, ANS, Dissection 17 16 Apr 28 General and Special Senses 18 17 Apr 30 Endocrine System 19 18 May 5 Lecture Exam and Practical 3 May 7 Circulatory System 1 20, 21 19 May 12 Circulatory System 2 21, 22 20 May 14 Lymphatic System, Dissection 23 21 May 19 Quiz, Respiratory System 24 22 May 21 Digestive System 25 23 May 26 Urinary System 26 24 May 28 Reproductive System 27 25 Jun 2 Lecture Exam and Practical 4 Jun 4 Review Jun 9 Final Exam 8 – 11am Anatomy 1 Lab Manual, Spring 2009 7 Exam Policies General Format: Lecture exams and lab practicals will be given on the same day. The exam will begin promptly at 8:30am and end at 10:50am. All students will begin with the lecture exam. You will be rotated into the lab practical at random. The practical lasts 30 minutes, after which you will return to your lecture exam. Lecture Exam: Lecture exams will consist of multiple choice, matching, and short answer questions. The multiple choice section will require a scantron. The short answer section includes lists, terminology, short essays, and drawing. Lecture exams are not cumulative. Lab Practical: The lab practical consists of 50 questions on histology, models, tissue, cat, and/or cadaver that you have seen in lab. It is not cumulative. It is organized in stations that are set up around the room. When you are signaled to begin the lab practical, you will pause your lecture exam and take a clipboard and answer sheet and begin the lab practical at any station. You have 30 minutes to complete it. Only one student per station. Do not touch the specimens or models unless instructed to do so. Do not touch the stage or the objective lenses on the microscopes. You may adjust the fine focus. If you have a question, raise your hand. You may not refer back to your lecture exam. When you are finished, turn in your answer sheet and return to your lecture exam. Final Exam: The final exam is cumulative. It consists of matching and short answer questions. It does not have an associated lab practical. You are allowed a cheat sheet: one side of one 8.5 x 11” paper, handwritten. Policies: All books, notes, and electronic devices must be left in your bag by the door. Use the restroom before the exam; you may not leave the room until you are finished with your exam. All exam work must be your own. Review: We will review the exam in class. The exams are my property, and may not leave the classroom in any form. I will return your exam to you in class and post the answers at the back of the classroom. You will have 15 minutes to review your exam and ask questions. You may not take notes, photograph, or leave the room with the exam. Any of these offenses will result in the filing of an Academic Dishonesty report. Leaving the room with the exam will also result in a loss of 10 points per minute that the exam is outside the classroom. If you need more time to review the exam, you are welcome to view it in my office. Anatomy 1 Lab Manual, Spring 2009 8 Lab 1: Introduction to Anatomy Lab Stations: 1. Identification: Torso model 2. Game: Simon says 3. Identification: Surface anatomy Lab Station 1: Identification: Torso model 1. Examine the human torso model to identify the structures listed below. Use your text as a reference. Answers are provided. Adrenal gland Brain Heart Kidneys Large intestine Liver Lungs Pancreas Small intestine Spinal cord Spleen Stomach Urinary bladder 2. Place each of the organs listed above in the correct body cavity. Dorsal body cavity: Thoracic cavity: Abdominopelvic cavity: 3. Assign each of these structures to an organ system. Digestive: Urinary: Cardiovascular: Endocrine: Respiratory: Lymphatic: Nervous: Anatomy 1 Lab Manual, Spring 2009 9 Lab Station 2: Game: Simon says Pick a leader; everybody else faces the leader. The leader gives instructions, but only those instructions preceded by "Simon Says" are to be followed. If someone follows an instruction that is not preceded by "Simon Says" they must leave the game. The last person remaining in the game other than the leader is the winner and will become the new leader in the next game. This is not a test – you may use your text as a reference. The leader picks from among these terms (you may specify right/left if you wish): Oculus Mentis Oris Cranium Cephalon Auris Buccal Nasus Cervicis Thoracis Lumbar Abdomen Pelvis Femur Hallux Phalanges Tarsus Planta Calcaneus Crural Sural Popliteus Patella Pollex Carpus Antebrachium Antecubitis Brachium Axilla Olecranon Acromial Lab Station 3: Identification: Surface anatomy For each of the labeled regions on the doll please specify the following. Possible answers are provided. This is the ___(name the anatomical landmark)______. It is located anterior to the ____(name another landmark)_____ , proximal to the ___(name another landmark)______, medial to the __(name another landmark)___ and superior to the _____(name another landmark)____. Anatomy 1 Lab Manual, Spring 2009 10 TERMS FOR LECTURE 1: Microscopic anatomy Histology Gross anatomy Chemical level Molecular level Cellular level Tissue level Organ level Organ system level Organism level Anatomical position Mentis Oris Cranium Cephalon Oculus Auris Bucca Nasus Cervicis Thoracis Mammary Abdomen Pelvis Inguen Pubis Femur Hallux Phalanges Tarsus Planta Calcaneus Crus Sura Popliteus Patella Pollex Carpus Antebrachium Antecubitis Brachium Axilla Gluteus Lumbar Olecranon Posterior Dorsal Anterior Ventral Superior Inferior Proximal Distal Medial Lateral Sagittal Frontal Coronal Transverse Dorsal cavity Cranial cavity Vertebral cavity Ventral cavity Pleural cavity Pericardial cavity Peritoneal cavity Abdominal cavity Pelvic cavity Anatomy 1 Lab Manual, Spring 2008 11 Lab 2: Cell Biology Lab Stations: 1. 2. 3. 4. Identification: Cell and mitosis models Drawing: Mitosis and Meiosis Game: Pictionary Histology: Introduction Lab Station 1: Identification: Cell and mitosis models 1. Identify the structures listed below on the cell model and in your text. Plasma membrane Centrioles Ribosomes Mitochondria Nucleus Endoplasmic reticulum Golgi apparatus Lysosomes 2. Examine the mitosis models and arrange them in order according to the cell cycle. Make sure to identify each phase. Mix them for the next group. 3. Please answer the following questions. Answers are provided. Which structure coats the outside of the cell? Which structure contains the genetic information for the cell? Which structures are involved in processing of proteins? Which structure is involved in removal of unwanted organelles or debris? Which structure is involved in making energy for the cell? Which structure allows recycling of plasma membrane? What is the base of the spindle fiber? Anatomy 1 Lab Manual, Spring 2008 12 Lab Station 2: Drawing: Mitosis and meiosis Use the paper and pens provided to draw both meiosis and mitosis. For the sake of clarity, follow only one homologous pair of chromosomes. Draw the chromosomes with two different colors. Follow the process through replication of those chromosomes, and division of the chromosomes and cells. Lab Station 3: Game: Pictionary The rules are flexible. You may form teams of 2 or 3 and designate the first drawer from each group (please take turns). The drawer picks a card and shares it with the drawer from the other group(s). The drawer has to draw the structure for his/her group and the group member(s) must guess what it is. The groups race against each other. No words may be used, and the drawer must be silent. You may keep score if you wish. This is not a test – the drawer may use the text to look up the structures, and you may decide as a group whether the guessers can use the text as well. Lab Station 4: Histology: Introduction The goal of this station is to become comfortable working with light microscopes. You will use these microscopes in almost every class and every exam. I expect you to use the microscope according to the following rules. 1. Identify the following parts of the microscope: base, arm, stage, power, light control, objective lenses, ocular lenses, course and fine focus knobs, stage controls. Use the handouts provided. 2. When handling a microscope, always move it using the base and arm only. 3. Turn the microscope on and make sure that there is light shining up through the stage. 4. Make sure that the stage is in the lowest position (as far down towards the lab bench as possible) and that there is no objective lens pointing down towards the stage. 5. Place a slide of the intestine in the slide holder on the stage, moving the stage so that light shines through the object on the slide. 6. Rotate the objective nosepiece so that the lowest power objective (the shortest one) is pointing towards the slide. 7. Use the course adjustment knob to focus the tissue. This is the only time you will use the course adjustment knob. 8. Rotate the objective nosepiece so that the middle power objective is pointing towards the slide. Now use the fine adjustment knob (the Anatomy 1 Lab Manual, Spring 2008 13 smaller one) to get this tissue in focus. Do not use the course adjustment knob, because you might crash the slide into the lens. 9. Rotate the objective nosepiece once again so that the highest power objective is pointing towards the slide. Use the fine adjustment knob to focus the tissue. 10. At the highest power objective you will be able to see the outlines of cells, and a darkly stained round nucleus inside. 11. Draw what you see from the intestinal cells at each of the 3 powers of magnification below. Become familiar with what you can see at each level of magnification, because this will be an important component in the lab practicals. Magnification: ______ Magnification: ______ Magnification: ______ What can you see? What can you see? What can you see? 12. When you are finished with this slide, reset the microscope by swiveling the objectives away and moving the stage all the way down. 13. Find a slide of mitosis and try to find a dividing cell and identify the phase of mitosis. 14. When you are finished using your microscope, reset the microscope by swiveling the objectives away and moving the stage all the way down. Improper use or storage of the microscope will result in loss of participation points. Anatomy 1 Lab Manual, Spring 2008 14 TERMS FOR LECTURE 2: Light microscopy Transmission electron microscopy Scanning electron microscopy Stage Base Arm Objective lens Ocular lens Adjustment knob Stage controls Extracellular fluid Intracellular fluid Cytoplasm Cytosol Organelles Plasma membrane Membrane proteins Semipermeable membrane Phospholipid bilayer Passive transport Active transport Cytoskeleton Microvilli Cilia Flagella Centrioles Ribosomes Mitochondria Nucleus Chromosomes Diploid Haploid Chromatin Centromere DNA Endoplasmic reticulum Golgi apparatus Intercellular cement Tight junctions Gap junction Interphase G0 G1 S G2 Mitosis Meiosis Prophase Metaphase Anaphase Telophase Spindle fibers Anatomy 1 Lab Manual, Spring 2008 15 Lab 3: Tissues Lab Stations: 1. Flowchart: Tissue types 2. Game: Chutes and ladders 3. Histology: Tissue Lab Station 1: Flowchart: Tissue types The goal of this project is to become comfortable with the hierarchy of tissue types. You may use the poster roll and markers provided, or draw on the white board. Sketch out a detailed flow chart of all the various tissue types and add as much detail as you have time for. Lab Station 2: Game: Chutes and ladders Each player must answer a trivia question before rolling the die. The trivia questions are taken from your text’s site: http://www.aw-bc.com/applace. There are two identical sets for the class. If you answer the question correctly, you may roll the die and move the appropriate number of steps forward. If you land on a ladder, you may climb up; if you land on a slide, you must slide down. Then the turn goes to the next player. The first to reach the end wins! You may decide as a group whether you can look at the text and/or lecture notes to answer the questions. Choose a reasonable time limit for each question (30 seconds?) and be lenient with answers. Lab Station 3: Histology: Tissue types Use the microscopes to view the tissue slides listed below. Make sure to become comfortable with the structure and function of the various tissue types, and practice predicting function from the structures that you see. Use your text and lecture notes as a reference. Epithelia slides: Simple squamous Stratified squamous Simple columnar Ciliated pseudostratified columnar Simple cuboidal Transitional Connective tissue slides: Adipose Areolar Dense regular Bone Cartilage (“hyaline”) Anatomy 1 Lab Manual, Spring 2008 16 TERMS FOR LECTURE 3: Epithelial tissue Glands Ciliated epithelium Apical Basal Basal lamina Simple squamous Stratified squamous Simple cuboid Stratified cuboid Transitional Simple columnar Pseudostratified columnar Stratified columnar Exocrine glands Endocrine glands Serous glands Mucous glands Merocrine secretion Apocrine secretion Holocrine secretion Lactiferous glands Sebaceous glands Connective tissue Matrix Ground substance Connective tissue proper Collagen Reticular fibers Elastic fibers Elastin Loose connective tissue Areolar tissue Adipose tissue Reticular tissue Dense connective tissue Dense regular connective tissue Dense irregular connective tissue Adipocytes Fluid connective tissue Blood Lymph Plasma Formed elements Red blood cells White blood cells Platelets Supporting connective tissue Bone Cartilage Chondrocytes Lacunae Cartilage Osteocytes Mucous membrane Serous membrane Cutaneous membrane Synovial membrane Muscle tissue Neural tissue Anatomy 1 Lab Manual, Spring 2008 17 Lab 4: Integumentary System Lab Stations: 1. 2. 3. 4. Identification: Skin model Game: Who wants to be a millionaire? Practice exam Histology: Hair, skin, nails, glands Lab Station 1: Identification: Skin model Identify the following structures both on the skin model and in your text: Epidermis Dermis Papillary layer Reticular layer Hair Hair follicles Exocrine glands Keratinocytes Make Melanocytes Langerhans cells Stratum corneum Stratum lucidum Stratum granulosum Stratum spinosum Stratum germinativum Epidermal ridges Dermal papillae Subcutaneous layer Hair root Shaft Sebaceous glands Sweat glands sure to discuss the following with your group: The function of each structure Where all of the main groups of tissues are found in skin The development of cells in the epidermis, from the basal cell layer to the outer layer that gets shed Lab Station 2: Game: Who wants to be a millionaire? (with minor modifications) Pick the first host and the first contestant. The host will pick a series of questions (one of the 8 stacks of cards) and will read the questions for the contestant, starting at the $100 question, and ending with the million dollar question. The contestant will try to answer the questions (fill-in-the-blank), and can continue answering questions until he/she get a question wrong. The questions get progressively more difficult, with an increasing dollar amount awarded as the questions get harder. For each question, the contestant may answer the question or take the money and run. If the contestant answers incorrectly, he/she gets the amount of (virtual) money from the previous question. Anatomy 1 Lab Manual, Spring 2008 18 The contestant may call ONCE upon each of 3 help strategies any time he/she chooses: 1. consult your book 2. ask the group 3. 50/50 chance (for this, the host must give the contestant 2 possible correct answers – so the host must make up an incorrect alternative) The roles of host and contestant rotate after each turn. Please choose a reasonable time limit for each question. Be lenient with the answers and ensure that this is a supportive learning environment for all. Please keep in the cards in order. Lab Station 3: Practice exam A practice exam will be laid out for you. Answers will be posted on the wall in the back of class. Lab Station 4: Histology: Hair, skin, nails, glands The goal of this station is to become comfortable with the structures of skin, hair, nails, and sweat glands. Use the dissecting microscopes to identify as many visible structures of your skin, hair, and nails as possible. For skin, the “corpuscle” slides are the best. Use a light microscope to view the slides of skin and scalp. Please make sure to discuss with your group the function of the various structures that you see. Use your text as a reference. Anatomy 1 Lab Manual, Spring 2008 TERMS FOR LECTURE 4: Integumentary system Cutaneous membrane Epidermis Dermis Papillary layer Reticular layer Subcutaneous layer Hypodermis Keratinocytes Keratin Melanocytes Melanin Langerhans cells Stratum corneum Basal cells Stratum lucidum Stratum granulosum Stratum spinosum Stratum germinativum Epidermal ridges Dermal papillae Basal cell carcinoma Squamous cell carcinoma Melanoma Accessory structures Hair follicles Hair matrix Hair root Shaft Club hair Exocrine glands Sebaceous glands Sweat glands Apocrine sweat glands Ceruminous glands Mammary glands Merocrine sweat glands Pheromones Nail Nail body Nail bed Nail root 19 Anatomy 1 Lab Manual, Spring 2008 20 Lab 5: Introduction to the Skeletal System Lab Stations: 1. Identification: Gross bone anatomy and bone markings 2. Game: Trivia 3. Histology: Bone and cartilage, and osteon model Lab Station 1: Identification: Gross bone anatomy and bone markings Look at the bones in front of you. Please identify all of the following on those bones, using your text and lecture notes as a resource. Long bone Flat bone Sutural bone Irregular bone Short bone Sesamoid bone Head Neck Condyle Trochlea Facet Process Ramus Trochanter Tuberosity Tubercle Crest Line Spine Fossa Sulcus Foramen Fissure Meatus Sinus Compact bone Spongy bone Trabeculae Periosteum Endosteum Epiphysis Metaphysic Diaphysis Hole for nutrient vessels Lab Station 2: Game: Trivia Please use the cards provided to quiz each other. This is not a test; you are welcome to use your text and your lecture notes. Anatomy 1 Lab Manual, Spring 2008 Lab Station 3: Histology: Bone and cartilage, and osteon model The goal of this station is to become comfortable with bone and cartilage histology. Use the microscope and slides provided to you to inspect the structure of compact bone and several types of cartilage. Use your text as a reference and identify: Ground bone: osteon, central canal, lacunae, lamellae, canaliculi Cartilage: lacunae, matrix Also look at the osteon model and identify the following: central canal, osteocytes, lacunae, lamellae, and canaliculi. 21 Anatomy 1 Lab Manual, Spring 2008 22 TERMS FOR LECTURE 5: Bone Cartilage Ligament Osseous tissue Calcium phosphate Osteoid Osteocytes Osteoblasts Osteoprogenitor cells Osteoclasts Chondrocytes Osteoporosis Compact bone Spongy bone Osteon Central canal Perforating canal Canal of Volkmann Lacunae Lamellae Canaliculi Trabeculae Periosteum Endosteum Epiphysis Metaphysic Diaphysis Epiphyseal plate Nutrient vessels Metaphyseal vessels Epiphyseal vessels Periosteal vessels Ossification Intramembranous Endochondral Primary ossification Secondary ossification Epiphyseal closure Vitamin D Parathyroid hormone Calcitonin Growth hormone Estrogen Testosterone Long bone Flat bone Sutural bone Irregular bone Short bone Sesamoid bone Head Neck Condyle Trochlea Facet Process Ramus Trochanter Tuberosity Tubercle Crest Line Spine Fossa Sulcus Foramen Fissure Meatus Sinus Anatomy 1 Lab Manual, Spring 2008 23 Lecture 6: Axial Skeleton Lab Stations: 1. Identification: Axial bones 2. Drawing: Axial skeleton Lab Station 1: Identification: Axial bones Look at the bones in front of you. Please identify all of the following, using your text and notes as a resource. Occipital: foramen magnum, occipital condyles, cerebellar fossa, cerebral fossa Parietal Frontal: supraorbital margin, frontal sinus Temporal: zygomatic process, mandibular fossa, external acoustic meatus, internal acoustic meatus, mastoid process, styloid process Sphenoid: greater wing, lesser wing, sella turcica Ethmoid: cribiform plate, crista galli, nasal concha, perpendicular plate Sagittal suture Coronal suture Lambdoidal suture Squamous suture Maxillae: maxillary sinus, palatine process, infraorbital foramen Palatine Nasal bone Nasal conchae Zygomatic: temporal process, frontal process Lacrimal Vomer Mandible: ramus, condylar process, coronoid process, mental foramen, mandibular foramen Hyoid bone Cervical vertebrae: transverse foramen Anatomy 1 Lab Manual, Spring 2008 Atlas Axis: dens Thoracic vertebrae Lumbar vertebrae Sacrum: sacral canal Coccyx On all vertebrae: vertebral body, vertebral arch, vertebral foramen, pedicle, laminae, spinous process, superior facet, inferior facet, transverse process, transverse facet Thoracic cage: true rib, false rib, floating rib, costal cartilage Rib: costal groove, tubercle, articular facets Sternum: manubrium, sternal body, xiphoid process 24 Lab Station 2: Drawing: Axial skeleton This is the start of a multi-week project. You will sketch out the bones of the body and later draw muscles over them. You may do this individually or as a group, and you may do this on letter-sized paper or on the poster rolls provided. Draw the skeleton twice, one anterior and one posterior view, so that muscles can be drawn onto the correct side. The key is to sketch the structures roughly, so that you have enough time to draw and label every bone and muscle. Please put your group’s names on the papers and carefully roll them up when you are done. I will keep them for you. Anatomy 1 Lab Manual, Spring 2008 25 TERMS FOR LECTURE 6: Cranium Occipital Foramen magnum Occipital condyles Cerebellar fossa Cerebral fossa Parietal Frontal Supraorbital margin Frontal sinus Temporal Zygomatic process Mandibular fossa External acoustic meatus Internal acoustic meatus Mastoid process Styloid process Sphenoid Greater wing Lesser wing Sella turcica Ethmoid Cribiform plate Crista galli Nasal concha Perpendicular plate Sagittal suture Coronal suture Lambdoidal suture Squamous suture Maxillae Maxillary sinus Palatine process Infraorbital foramen Palatine Nasal bone Nasal conchae Zygomatic Temporal process Frontal process Lacrimal Vomer Xiphoid process Mandible Ramus Condylar process Coronoid process Mental foramen Mandibular foramen Hyoid bone Vertebral column Cervical Thoracic Lumbar Sacral Coccyx Vertebral body Vertebral arch Vertebral foramen Transverse foramen Pedicle Laminae Spinous process Superior facet Inferior facet Transverse process Transverse facet Intervertebral disc Intervertebral foramen Bifid Atlas Axis Dens Sacral canal True rib False rib Floating rib Costal cartilage Costal groove Tubercle Articular facets Sternum Manubrium Sternal body Anatomy 1 Lab Manual, Spring 2008 26 Lab 7: Appendicular Skeleton Lab Stations: 1. Identification: Appendicular bones 2. Drawing: Appendicular bones Lab Station 1: Identification: Appendicular bones Look at the bones in front of you. Please identify all of the following, using your text and notes as a resource. Clavicle: sternal end, acromial end, sternal facet Scapula: spine, supraspinous fossa, infraspinous fossa, glenoid fossa, acromion, coracoid process, supraglenoid tubercle, infraglenoid tubercle Humerus: head, greater tubercle, lesser tubercle, intertubercular sulcus, deltoid tuberosity, condyles, medial epicondyle, lateral epicondyle, trochlea, coronoid fossa, olecranon fossa, capitulum Ulna: olecranon, trochlear notch, coronoid process, radial notch, interosseous membrane, ulnar styloid process Radius: radial styloid process, ulnar notch, radial tuberosity Carpal bones: scaphoid, lunate, triquetrum, pisiform, trapezium, trapezoid, capitate, hamate Metacarpal bones Phalanges: pollex Ossa coxae: ilium, ischium, pubis, posterior superior iliac spine, posterior inferior iliac spine, greater sciatic notch, ischial spine, lesser sciatic notch, ischial tuberosity, iliac crest, anterior superior iliac spine, anterior inferior iliac spine, acetabular fossa, obturator foramen, pubic symphysis, iliac fossa Femur: head, fovea, greater trochanter, lesser trochanter, medial epicondyle, lateral epicondyle, condyles, patellar surface, gluteal tuberosity, linea aspera Patella: apex, base, medial facet, lateral facet Tibia: medial condyle, lateral condyle, tibial tuberosity, anterior margin, crural interosseous membrane, medial malleolus Fibula: lateral malleolus Tarsal bones: talus, calcaneus, cuboid, navicular, cuneiform Anatomy 1 Lab Manual, Spring 2008 Metatarsal bones Phalanges: hallux Lab Station 2: Drawing This is the continuation of a multi-week project. Get your drawing from me, and add the entire appendicular skeleton to it. 27 Anatomy 1 Lab Manual, Spring 2008 28 TERMS FOR LECTURE 7: Pelvic girdle Pectoral girdle Clavicle Sternal facet Scapula Spine Supraspinous fossa Infraspinous fossa Glenoid fossa Acromion Coracoid process Supraglenoid tubercle Infraglenoid tubercle Humerus Head Greater tubercle Lesser tubercle Intertubercular sulcus Deltoid tuberosity Condyles Medial epicondyle Lateral epicondyle Trochlea Coronoid fossa Olecranon fossa Capitulum Ulna Olecranon Trochlear notch Coronoid process Radial notch Interosseous membrane Ulnar styloid process Radius Radial styloid process Ulnar notch Radial tuberosity Carpal bones Scaphoid Lunate Triquetrum Pisiform Trapezium Trapezoid Capitate Hamate Metacarpal bones Phalanges Pollex Ossa coxae Ilium Ischium Pubis Posterior superior iliac spine Posterior inferior iliac spine Greater sciatic notch Ischial spine Lesser sciatic notch Ischial tuberosity Iliac crest Anterior superior iliac spine Anterior inferior iliac spine Acetabular fossa Obturator foramen Pubic symphysis Iliac fossa Femur Head Fovea Greater trochanter Lesser trochanter Medial epicondyle Lateral epicondyle Condyles Patellar surface Gluteal tuberosity Linea aspera Patella Apex Base Medial facet Lateral facet Tibia Medial condyle Lateral condyle Tibial tuberosity Anterior margin Crural interosseous membrane Medial malleolus Fibula Lateral malleolus Tarsal bones Talus Calcaneus Cuboid Navicular Cuneiform Metatarsal bones Phalanges Hallux Arches Anatomy 1 Lab Manual, Spring 2008 29 Lab 8: Articulations Lab Stations: 1. Identification: Sample joints and accessory structures 2. Game: Charades 3. Identification: Joints on a skeleton FINAL ACTIVITY: ARTICULATIONS BINGO Lab Station 1: Identification: Sample joints and accessory structures Identify all of the ligaments on the joint models provided. If you finish early, you may work on your skeleton drawings or on the handout on the next page. Lab Station 2: Game: Charades Designate the first performer. That person picks a card from the stack and must act out the term without using words. The rest of the group tries to guess the term. The winner becomes the next performer. This is not a test – the drawer may use the text to look up the terms. Anatomy 1 Lab Manual, Spring 2008 30 Lab Station 3: Identification: Joints on a skeleton Look at the skeleton. Identify all of these joints and list what type of joint it is: synarthrosis, amphiarthrosis, or diarthrosis. If it is a diarthrosis, list what type of synovial joint it is: plane, hinge, pivot, condylar, saddle, ball and socket. If it is amphiarthrosis or diarthosis do not identify type. Try to palpate or move these joints on your own body, to acquaint yourself with the range of motion provided by each joint. An example is provided. JOINT Cranial sutures Temperomandibular joint Atlantooccipital joint Atlantoaxial joint Intervertebral discs Intervertebral facets Glenohumeral joint Humeroulnar joint Interosseous membrane Distal radioulnar joint Radiocarpal joint 1st Carpometacarpal joint 2-5 Carpometacarpal joint Metacarpophalangeal joint Interphalangeal joint Hip joint Pubic joint Tibiofemoral joint Tibiotalar joint Tarometatarsal joints Metatarsophalangeal joints TYPE Diarthrosis SYNOVIAL TYPE Hinge Anatomy 1 Lab Manual, Spring 2008 31 TERMS FOR LECTURE 8: Range of motion Synarthrosis Amphiarthrosis Diarthrosis Suture Synovial joint Joint capsule Articular cartilage Synovial fluid Synovial membrane Meniscus Bursae Gliding Abduction Adduction Flexion Extension Internal rotation External rotation Medial rotation Lateral rotation Pronation Supination Eversion Inversion Dorsiflexion Plantar flexion Lateral flexion Protraction Retraction Elevation Depression Circumduction Plane joint Gliding joint Hinge joint Pivot joint Condylar joint Saddle joint Ball and socket joint You need to be familiar with these joints: Temperomandibular joint Intervertebral joints Intervertebral disc Glenohumeral joint Humeroulnar joint Humeroradial joint Radiocarpal joint Intercarpal joint Carpometacarpal joint Metacarpophalangeal joint Interphalangeal joint Hip joint Tibiofemoral joint Patellofemoral joint Talocrural joint Tibiotalar joint Fibiotalar joint Intertarsal joints Tarometatarsal joints Metatarsophalangeal joints Interphalangeal joints You need to identify the following structures, given the name: Glenoid labrum Glenohumeral ligaments Coracohumeral ligaments Coracoclavicular ligaments Acromioclavicular ligaments Coracoacromial ligaments Subdeltoid bursa Subacromial bursa Subcoracoid bursa Annular ligament Ulnar collateral ligament Radial collateral ligament Acetabulum Acetabular labrum Ligament of femoral head Pubofemoral ligament Iliofemoral ligament Ischiofemoral ligament Fibular collateral ligament Tibial collateral ligament Patellar ligament Anterior cruciate ligament Posterior cruciate ligament Infrapatellar fat pad Anatomy 1 Lab Manual, Spring 2008 32 Lab 9: Introduction to Muscular System Lab Stations: 1. Identification: Muscle model and sarcomere drawing 2. Game: Trivia 3. Histology: Skeletal, cardiac, and smooth muscle Lab Station 1: Identification: Muscle model and sarcomere drawing 1. Look at the muscle model and identify the following: fascicle, muscle fiber, myofibril, sarcomere, motor neuron, neuromuscular junction 2. Practice drawing a sarcomere and discuss with your group how the filaments move in relation to each other and the effect that contraction has on the structure of the sarcomere. Lab Station 2: Game: Trivia Please use the cards provided to quiz each other. This is not a test; you are welcome to use your text and your lecture notes. Lab Station 3: Histology: Skeletal, cardiac, and smooth muscle Look at the following slides and make sure that you can identify the following structures: Skeletal muscle: muscle fiber, striations Cardiac muscle: muscle fiber, striations, intercalated discs Smooth muscle: muscle fiber, lack of striations Neuromuscular junction: muscle fiber, motor neuron, neuromuscular junction, axon Note: the purple-stained muscles slides are the most clear. Anatomy 1 Lab Manual, Spring 2008 TERMS FOR LECTURE 9: Epimysium Perimysium Endomysium Satellite cells Skeletal muscle Cardiac muscle Smooth muscle Gap junctions Intercalacted discs Fascicle Muscle fiber Myofibrils Filaments Sarcoplasm Striations Sarcomere Myosin Actin Sliding filament theory A band I band Z disk Motor neuron Motor unit Axon terminal Neuromuscular junction Motor unit recruitment Neuromuscular cleft Motor end plate Parallel muscles Convergent muscles Unipennate muscles Bipennate muscles Multipennate muscles Circular muscles Origin Insertion Action Prime mover Agonist Synergist Antagonist 33 Anatomy 1 Lab Manual, Spring 2008 34 Lab 10: Axial Musculature Lab Stations: 1. Identification: Muscle model 2. Drawing: Axial muscles Lab Station 1: Identification: Muscle models Look at the muscle models provided. Identify all of the muscles in the “terms” list and note their origin, insertion, and action. If possible, demonstrate the action on yourselves. If a muscle cannot be identified on a model, please use your text. For your convenience, the muscles that you need to know are listed again with their origin, insertion, and action on pages 38-43. If time remains, you can start to build your flashcard library. Lab Station 2: Drawing: Axial muscles Add all of the muscles from the “terms” list to your drawing. Anatomy 1 Lab Manual, Spring 2008 TERMS FOR LECTURE 10: Identification and action only: Occipitofrontalis Orbicularis oris Orbicularis oculi Buccinator Platysma Risorius Zygomaticus major Zygomaticus minor Masseter Temporalis Digastric Identification, origin, insertion, and action of: Sternocleidomastoid Iliocostalis Longissimus Spinalis Quadratus lumborum Rotatores Interspinales Intertransversarii External oblique Internal oblique Transversus abdominis Rectus abdominis Trapezius Pectoralis minor Levator scapula Rhomboid major Rhomboid minor Serratus anterior Deltoid Pectoralis major Subscapularis Teres major Coracobrachialis Supraspinatus Latissimus dorsi Teres minor Infraspinatus 35 Anatomy 1 Lab Manual, Spring 2008 36 Lab 11: Appendicular Musculature Lab Stations: 1. Identification: Muscle model 2. Drawing: Appendicular muscles Lab Station 1: Identification: Muscle models Look at the muscle models provided. Identify all of the muscles in the “terms” list and note their origin, insertion, and action. If possible, demonstrate the action on yourselves. If a muscle cannot be identified on a model, please use your text. For your convenience, the muscles that you need to know are listed again with their origin, insertion, and action on pages 38-43. If time remains, you can start to build your flashcard library. Lab Station 2: Drawing: Appendicular muscles Add all of the muscles from the “terms” list to your drawing. Anatomy 1 Lab Manual, Spring 2008 37 TERMS FOR LECTURE 11: You must know the origin, insertion, and action of all of the following muscles: Brachioradialis Biceps brachii Brachialis Triceps brachii Flexor carpi radialis Palmaris longus Flexor carpi ulnaris Flexor digitorum superficialis Extensor carpi radialis longus Extensor carpi ulnaris Extensor carpi radialis brevis Extensor digitorum Gluteus maximus Gluteus medius Gluteus minimus Lateral rotators Iliacus Psoas major Tensor fasciae latae Adductor longus Adductor magnus Gracilis Rectus femoris Vastus lateralis Vastus intermedius Vastus medialis Sartorius Biceps femoris Semitendinosus Semimembranosus Gastrocnemius Soleus Flexor digitorum longus Flexor hallucis longus Fibularis longus Fibularis brevis Tibialis anterior Anatomy 1 Lab Manual, Spring 2008 38 For the following muscles, you are only responsible for identifying them on a model and stating their actions. MUSCLE Occipitofrontalis ORIGIN Cranium INSERTION Eyebrow skin Orbicularis oris Lips Risorius Zygomaticus major Maxilla and mandible Orbit Maxilla and mandible Ribs and acromion Fascia Zygomatic bone Zygomaticus minor Zygomatic bone Upper lip Masseter Temporalis Zygomatic arch Parietal bone Digastric Mandible and mastoid process of temporal bone Mandibular ramus Coronoid process of mandible Hyoid Orbicularis oculi Buccinator Platysma Eyelid skin Orbicularis oris Mandible and skin Mouth Mouth ACTION Raises eyebrows Compresses lips Closes eye Compresses cheek Tenses skin of neck Mouth grimace Elevates mouth Elevates upper lip Closes jaw Closes jaw Opens jaw Anatomy 1 Lab Manual, Spring 2008 39 For the remainder of the muscles on this list, you are responsible for all of this information. MUSCLE Sternocleidomastoid Iliocostalis Longissimus Spinalis Quadratus lumborum Rotatores Interspinales Intertransversarii External oblique ORIGIN Clavicle and manubrium Iliac crest and ribs Transverse processes Spinous processes Iliac crest Transverse processes Spinous processes Transverse processes Ribs Internal oblique Iliac crest and thoracolumbar fascia Transversus abdominis Ribs and iliac crest Rectus abdominis Pubis Trapezius Pectoralis minor Levator Scapula Rhomboid major and minor Serratus anterior Occipital bone, spinous processes of Cspine Ribs Transverse processes of Cspine Spinous processes of upper Tspine Anterior ribs INSERTION Mastoid process of temporal bone Ribs and transverse processes Transverse processes Spinous processes Ribs and transverse processes Spinous processes ACTION Flex neck Spinous processes Transverse processes External oblique aponeuroses Extends spine Lateral flexion of spine Flex and rotate spine to opposite side Flex and rotate spine to same side Compress abdomen Flex spine Ribs and linea alba Linea alba Cartilages of lower ribs and xiphoid process Clavicle and acromion/spine of scapula Coracoid process Scapula Scapula Scapula Extends spine Extends spine Extends spine Lateral spine flexion Rotates spine Elevate and retract scapula Depress and protract scapula Elevates scapula Adducts scapula Protracts scapula Anatomy 1 Lab Manual, Spring 2008 MUSCLE Deltoid 40 ORIGIN Clavicle and spine/acromion of scapula Ribs, sternum and clavicle INSERTION Deltoid tuberosity of humerus ACTION Abducts shoulder Greater tubercle of humerus Teres major Scapula Humerus Coracobrachialis Coracoid process Humerus Latissimus dorsi Spinous processes of lower spine, thoracolumbar fascia Humerus Subscapularis Subscapular fossa Lesser tubercle of humerus Supraspinatus Greater tubercle Teres minor Supraspinous fossa Scapula Infraspinatus Infraspinous fossa Greater tubercle of humerus Brachioradialis Lateral epicondyle of humerus Coracoid process and supraglenoid tubercle Humerus Humerus, infraglenoid tubercle Medial epicondyle of humerus Medial epicondyle of humerus Styloid process of radius Flexion, adduction of shoulder Extension, adduction, medial rotation of shoulder Shoulder flexion Extension, adduction, medial rotation of shoulder Medial rotation of shoulder Abduction of shoulder Lateral rotation of shoulder Lateral shoulder rotation Elbow flexion Pectoralis major Biceps brachii Brachialis Triceps brachii Flexor carpi radialis Flexor carpi ulnaris Greater tubercle of humerus Radial tuberosity Ulna Olecranon Metacarpals Metacarpals Elbow and shoulder flexion Elbow flexion Elbow and shoulder extension Wrist flexion and abduction Wrist flexion and adduction Anatomy 1 Lab Manual, Spring 2008 MUSCLE Palmaris longus Flexor digitorum superficialis Extensor carpi radialis longus Extensor carpi radialis brevis ORIGIN Medial epicondyle of humerus Medial epicondyle of humerus Humerus 41 INSERTION Flexor retinaculum Phalanges ACTION Wrist flexion IT band Abduction of hip Adduction of hip Adduction of hip Adducts hip, flexes knee Knee extension, hip flexion Knee extension Wrist and finger flexion Metacarpals Wrist extension, abduction Metacarpals Wrist extension and abduction Metacarpals Wrist extension and adduction Phalanges Finger and wrist extension Gluteal tuberosity Extends hip of femur Greater Abducts hip trochanter of femur Greater Abducts hip trochanter of femur Femur Lateral rotation Lesser trochanter Hip flexion Lesser trochanter Hip flexion Gluteus maximus Lateral epicondyle of humerus Lateral epicondyle of humerus Lateral epicondyle of humerus Iliac crest Gluteus medius Iliac crest Gluteus minimus Ilium Lateral rotators Ischial spine Iliacus Psoas major Adductor longus Iliac fossa Lower transverse processes Iliac crest and ASIS Inferior pubis Adductor magnus Inferior pubis Gracilis Inferior pubis Linea aspera of femur Linea aspera of femur Medial tibia Rectus femoris AIIS Tibial tuberosity Vastus lateralis Femur Tibial tuberosity Extensor carpi ulnaris Extensor digitorum Tensor fasciae latae Anatomy 1 Lab Manual, Spring 2008 42 MUSCLE Vastus intermedius ORIGIN Femur INSERTION Tibial tuberosity Vastus medialis Femur Tibial tuberosity Sartorius ASIS Medial tibia Biceps femoris Fibula and tibia Semitendinosus Ischial tuberosity and linea aspera Ischial tuberosity Semimembranosus Ischial tuberosity Tibia Gastrocnemius Femoral condyles Calcaneous Soleus Flexor digitorum longus Flexor hallucis longus Fibula and tibia Tibia and fibula Calcaneous Phalanges Fibula Phalanx of toe Fibularis longus and brevis Tibialis anterior Fibula Metatarsals ACTION Knee extension Knee extension Knee flexion, hip flexion and lateral rotation Knee flexion, hip extension Knee flexion, hip extension Knee flexion, hip extension Plantar flexion at ankle, knee flexion Plantar flexion Toe flexion, plantar flexion Hallux flexion, plantar flexion Plantar flexion Tibia Metatarsals Dorsiflexion Tibia Anatomy 1 Lab Manual, Spring 2008 43 Lab 12: Cat Dissection Over the next few weeks, you will progressively dissect a cat in order to learn basic dissection skills and to become familiar with anatomical structures as they appear in nature. The dissections are required. You will be performing these dissections in groups of 3-4, and as a group, you will need 2 dissecting kits, gloves, a lock for your locker, and individual protective garments. Basic rules: 1. Watch out for each other. Be aware at all times of your dissection area and make sure that your lab partners’ hands are nowhere in the vicinity of your work. Also be supportive of any potential emotional discomfort that may arise when dissecting an animal. 2. Protect your skin: wear gloves at all times. Do not touch any part of your body with your gloves on; particularly not your eyes. 3. Keep your instruments down on the table when you are not using them. 4. Use primarily blunt dissection. We will not be using the scalpels at all, and the scissors will rarely be used for cutting. To start: 1. Obtain a cat from me. Cut the bag open and dump the fluid into the bins provided. Place the cat onto a tray and immediately cover the face with paper towels. When everybody is ready, we will begin together. 2. Orient yourself to your cat. Your cat is already skinned. Palpate the xyphoid process. Palpate the calcaneous. 3. We will all start by dissecting the back muscles. Lie your cat face down. Find the dividing line between the trapezius and latissiums dorsi, and begin to separate the two muscles from each other, using blunt dissection. If there is a lot of fat on your cat, you will need to spend some time removing the fat. 4. When these muscles have been dissected, I will designate a region for your group to work on. Use the images provided as a reference, but please do not touch them. To clean up: 1. Place your cat back in its bag, fold the top over and rubber band it shut. Place a name tag on the bag with a label that includes your group member’s names, your Anatomy section time, and my name. Write this information another time on the bag itself. Place the cat on its rack. 2. Clean your trays, dissection equipment, and lab bench with simple green. Be careful with your dissection equipment: use as little water as possible and always pat dry. 3. Wash your hands. 4. Make sure that all surfaces and sinks are clean, and that your bench is back in its original position before leaving. Anatomy 1 Lab Manual, Spring 2008 Muscles to Identify on the Cat: Abdominal: Rectus abdominis External oblique Chest: Pectoralis major and minor (major is superior to minor) Back: Trapezius (3 separate muscles on cat) Latissimus dorsi Shoulder: Deltoid (3 separate muscles on cat) Elbow: Biceps brachii Triceps brachii Brachioradialis Wrist: flexors and extensors Hip and knee: Gluteus maximus Tensor fasciae latae Gracilis Sartorius Adductors Rectus femoris Vastus lateralis Vastus medialis Vastus intermedius Biceps femoris Semitendinosus Semimembranosus Ankle: Gastrocnemius Soleus Tibialis anterior Fibularis longus and brevis Dorsiflexors Plantarflexors 44 Anatomy 1 Lab Manual, Spring 2008 45 Lab 13: Introduction to Nervous System Lab Stations: 1. Game: Pictionary 2. Identification: Connective tissue protection 3. Histology: Neurons and neuron model Lab Station 1: Game: Pictionary The rules are flexible. You may form teams of 2 or 3 and designate the first drawer from each group (please take turns). The drawer picks a card and shares it with the drawer from the other group(s). The drawer has to draw the structure for his/her group and the group member(s) must guess what it is. The groups race against each other. No words may be used, and the drawer must be silent. You may keep score if you wish. This is not a test – the drawer may use the text to look up the structures, and you may decide as a group whether you would like the guessers to have access to the text as well. Lab Station 2: Identification: Connective tissue protection Look at the brain, spinal cord, and nerve provided for you. Make sure that you can identify: Cranial meninges Spinal meninges Dura mater Endosteal layer Meningeal layer Dural sinus Falx cerebri Tentorium cerebelli Falx cerebelli Arachnoid mater Arachnoid granulations Pia mater Filum terminale Denticulate ligaments Epineurium Anatomy 1 Lab Manual, Spring 2008 46 Lab Station 3: Histology and neuron model Look at the slides of giant multipolar neurons provided. Make sure that you can identify dendrites, cell body, and axons. Next, look at the neuron model and identify dendrites, cell body, axon, and synaptic terminal. Be sure you can identify the pre- and post-synaptic sides of the cell. Anatomy 1 Lab Manual, Spring 2008 47 TERMS FOR LECTURE 13: Central nervous system Brain Spinal cord Peripheral nervous system Neurons Supporting cells Glia Dendrites Cell body Axon Synaptic terminal Myelin Multiple sclerosis Guillain-barre syndrome Synapse Synaptic knob Neurotransmitter Synaptic vesicles Synaptic cleft Postsynaptic membrane Afferent Efferent Somatic Visceral Nuclei Centers Tracts Columns Ganglia Nerves Cranium Cranial meninges Spinal meninges Meningitis Dura mater Endosteal layer Meningeal layer Dural sinus Falx cerebri Tentorium cerebelli Falx cerebelli Epidural hemorrhage Subdural hemorrhage Arachnoid mater Arachnoid trabeculae Arachnoid granulations Arachnoid villi Subdural space Subarachnoid space Cerebral spinal fluid Pia mater Filum terminale Denticulate ligaments Epineurium Perineurium Endoneurium Anatomy 1 Lab Manual, Spring 2008 48 Lab 14: Brain Lab Stations: 1. Dissection: Sheep brains 2. Identification: Human brain tissue and models 3. Cranial nerves Lab Station 1: Dissection: Sheep brains 1. Organize yourselves into two groups. Each group should get gloves, a small dissecting tray, and a sheep brain. 2. Orient yourself to the brain: which side is ventral and which is dorsal? Anterior and posterior? Make sure that you can identify the cerebrum, cerebellum, midbrain, pons, medulla oblongata, optic chiasm, mammillary bodies, pineal gland, and corpora quadrigemina. Use the images provided as a guide, but please only touch them with clean hands. 3. One group should cut the brain midsagitally and identify the corpus callosum, thalamus, and substantia nigra of the midbrain. Then cut several more sagittal sections, approximately every inch in both directions. View the sections to find where the basal ganglia are located in relation to the thalamus. 4. The second group should cut the brain coronally every inch across the entire brain. Identify the basal ganglia, thalamus, and ventricles in their respective sections. Anatomy 1 Lab Manual, Spring 2008 49 Lab Station 2: Identification: Human brain tissue and models You will be shown a brain model and a real human brain. Please identify all of the following terms: Lateral ventricles Third ventricle Fourth ventricle Cerebrum Cerebral hemisphere Gyri Sulci Longitudinal fissure Central sulcus Lateral sulcus Frontal lobe Parietal lobe Occipital lobe Temporal lobe Precentral gyrus Postcentral gyrus Internal capsule Corpus callosum Basal ganglia Thalamus Hypothalamus Pituitary gland Mamillary bodies Pineal gland Midbrain Corpora quadrigemina Pons Cerebellar peduncle Medulla oblongata Pyramids Olives Cerebellum Olfactory bulb Olfactory tract Optic nerve Optic chiasm Optic tract Oculomotor nerve Trigeminal nerve Vagus nerve Accessory nerve Hypoglossal nerve Anatomy 1 Lab Manual, Spring 2008 50 Lab Station 3: Cranial Nerves Cranial nerve tests are an important part of any neurological examination. Conduct the following tests of cranial nerve function to help you learn cranial nerve function. As you are doing these tests, please review the path of the nerve, whether it is motor or sensory, and what its main function is. Please note that cranial nerves are part of the peripheral nervous system, because they travel outside the brain. Once inside the brain they travel in tracts. 1. Olfactory – ask your partner whether they can smell a substance: soap 2. Optic – test the point at which your partner first sees an object moving in the visual field 3. Oculomotor – ask your partner to follow your moving finger 4. Trochlear – ask your partner to look down on his/her nose 5. Trigeminal – ask your partner to close his/her eyes. Lightly touch his/her forehead, lower orbit, and lower mandible, and make sure that he/she can feel it 6. Abducens – ask your partner to look side to side 7. Facial – ask your partner to smile, frown, and raise eyebrows 8. Vestibulocochlear – snap your fingers behind your partner’s head and ask whether he/she can hear it. Then ask your partner to stand on one leg and check for balance 9. Glossopharyngeal – if tongue depressors are available, check for the gag reflex by touching the posterior aspect of the tongue 10. Vagus – ask your partner to open wide and say “AH” (vagus innervates many organs, including the larynx) 11. Accessory – test your partner’s shoulder shrug strength and ability to turn head to the side against resistance (accessory nerve innervates pharynx and larynx, and also trapezius and SCM muscles) 12. Hypoglossal – ask your partner to stick out his/her tongue Anatomy 1 Lab Manual, Spring 2008 51 TERMS FOR LECTURE 14: Gray matter White matter Ventricles Lateral ventricles Interventricular foramen Third ventricle Cerebral aqueduct Fourth ventricle Central canal Cerebrospinal fluid Lateral aperture Medial aperture Hydrocephalus Cerebrum Cerebral hemisphere Gyri Sulci Fissures Longitudinal fissure Central sulcus Lateral sulcus Parietooccipital sulcus Frontal lobe Parietal lobe Occipital lobe Temporal lobe Precentral gyrus Postcentral gyrus Primary motor cortex Primary sensory cortex Visual cortex Auditory cortex Association areas Internal capsule Corpus callosum Basal ganglia Thalamus Hypothalamus Pituitary gland Mamillary bodies Pineal gland Midbrain Corpora quadrigemina Substantia nigra Parkinson’s disease Pons Cerebellar peduncle Medulla oblongata Pyramids Olives Cerebellum Olfactory nerve Olfactory bulb Olfactory tract Optic nerve Optic chiasm Optic tract Oculomotor nerve Trochlear nerve Trigeminal nerve Abducens nerve Facial nerve Vestibulocochlear nerve Glossopharyngeal nerve Vagus nerve Accessory nerve Hypoglossal nerve Anatomy 1 Lab Manual, Spring 2008 52 Lab 15: Spinal Cord and PNS Lab Stations: 1. Drawing: Spinal nerves 2. Identification: Spinal cord specimen and model 3. Drawing: Spinal cord tracts FINAL ACTIVITY: NEUROBINGO Lab Station 1: Drawing: Spinal nerves Use the paper and pens provided to very roughly trace the path of the following nerves, starting from the spinal nerves that exit the spinal cord, going through the plexus, and to the target. You do not need to draw or study the plexi in detail. Please use your text, the thin man (large drawing), and models to visualize where each of these nerves travels. Phrenic nerve Musculocutaneous nerve Radial nerve Median nerve Ulnar nerve Femoral nerve Sciatic nerve Saphenous nerve Common fibular nerve Tibial nerve Lab Station 2: Identification: Spinal cord specimen and model I will present a human spinal cord to you, and show you the cauda equina, spinal cord enlargements, posterior median fissure, and anterior median fissure. I will then show you a spinal cord model, where you will see the following structures. I will also transect a sheep spinal cord to show you those structures on a real specimen. Central gray matter Posterior gray horn Lateral gray horn Anterior gray horn Outer white matter Funiculi / Columns Posterior white column Fasciculus gracilis Fasciculus cuneatus Central canal Ventral root Dorsal root Dorsal root ganglion Spinal nerve Anatomy 1 Lab Manual, Spring 2008 53 Lab Station 3: Drawing: Spinal cord tracts Please use the pens and paper provided to draw the path of neurons in the posterior white column and the corticospinal tracts. For the sensory tract, please start at the sensation in your limb. For the motor tract, please start with the motor cortex. Use different color markers for the different neurons (3 for the sensory pathway and 2 for the motor pathway) and make sure to show where the synapses are and where they cross over from one side of the body to the other. Anatomy 1 Lab Manual, Spring 2008 54 TERMS FOR LECTURE 15: Cauda equina Spinal tap Spinal segment Innervation Central gray matter Outer white matter Ascending tracts Descending tracts Columns Posterior median sulcus Anterior median fissure Central canal Ventral root Dorsal root Dorsal root ganglion Spinal nerve Posterior gray horn Lateral gray horn Anterior gray horn Sensory nuclei Motor nuclei Funiculi Posterior white column Fasciculus gracilis Fasciculus cuneatus Nucleus gracilis Nucleus cuneatus Homunculus Lateral corticospinal tract Anterior corticospinal tract Upper motor neuron Lower motor neuron Quadriplegia Paraplegia Ventral rami Dorsal rami Cervical plexus Brachial plexus Lumbar plexus Sacral plexus Phrenic nerve Trunks Cords Musculocutaneous nerve Radial nerve Median nerve Ulnar nerve Carpal tunnel syndrome Femoral nerve Lumbosacral trunk Sciatic nerve Saphenous nerve Common fibular nerve Tibial nerve Sciatica Dermatome Myotome Reflexes Anatomy 1 Lab Manual, Spring 2008 55 Lab 16: Autonomic Nervous System Lab Activities: 1. Flow chart: Nervous system organization 2. Dissection Lab Activity 1: Flow chart: Nervous system organization Use the pens and paper provided to draw a flow chart of the nervous system. Make sure to include the following: Nervous System Central nervous system Peripheral nervous system Sensory afferent Motor efferent Somatic Visceral Parasympathetic Sympathetic Smooth muscle Cardiac muscle Glands Skeletal muscles Skin Joints Lab Activity 2: Cat Dissection We will dissect the brachial plexus and sciatic nerve. Anatomy 1 Lab Manual, Spring 2008 TERMS FOR LECTURE 16: Autonomic nervous system Preganglionic Postganglionic Sympathetic nervous system Parasympathetic nervous system Thoracolumbar division Craniosacral division Terminal ganglia Sympathetic chain ganglia Paravertebral ganglia Rami communicantes Gray ramus White ramus Collateral sympathetic ganglia Splanchic nerves Adrenal gland Fight or flight Rest and digest Epinephrine Norepinephrine Adrenaline Noradrenaline Acetylcholine Cholinergic Adrenergic Synapses en passant Antagonistic responses Dual innervation Autonomic plexuses Visceral reflexes 56 Anatomy 1 Lab Manual, Spring 2008 57 Lab 17: General and Special Senses Lab Stations: 1. Identification: Auditory and vestibular models 2. Identification: Eye models 3. Histology: General and special senses Lab Station 1: Identification: Auditory and vestibular models Use the models and the text to identify the following: External ear Middle ear Inner ear Auricle External auditory meatus Tympanic membrane Tympanic cavity Auditory ossicles Malleus Incus Stapes Auditory tube Round window Oval window Bony labyrinth Membranous labyrinth Semicircular canals Vestibule Cochlea Perilymph Endolymph Ampulla Utricle Saccule Vestibular duct (scala vestibuli) Vestibular membrane Cochlear duct (scala media) Tectorial membrane Basilar membrane Tympanic duct (scala tympani) Organ of corti Anatomy 1 Lab Manual, Spring 2008 58 Lab Station 2: Identification: Eye models Use the models of the eye and your text to identify all of the following: Cornea Iris Pupil Lens Ciliary body Suspensory ligaments Sclera Choroid Retina Fovea Optic disc Retinal epithelium Rod Cone Retinal ganglion cell Lab Station 3: Histology: General and special senses Please look at the slides provided and identify the following. Use your text, notes, and the images provided as a reference. Tactile corpuscles Cochlea: cranial nerve 8, vestibular duct, cochlear duct, tympanic duct, tectorial membrane, basilar membrane, vestibular membrane, hair cells, organ of Corti, endolymph, perilymph Crista ampullaris: crista, hair cells, cupula, endolymph Retina: sclera, choroid, retinal epithelium, rod/cone nuclei, bipolar cells, retinal ganglion cells Anatomy 1 Lab Manual, Spring 2008 59 TERMS FOR LECTURE 17: General senses Proprioception Special senses Olfaction Gustation Vestibular Auditory Sensory receptors Chemoreceptors Thermoreceptors Mechanoreceptors Nociceptors Referred pain Baroreceptors Free nerve endings Merkel’s discs Ruffini endings Tactile corpuscules Lamellated corpuscules Muscle spindles Golgi tendon organ Olfactory bulb Olfactory cortex Taste cells Taste bud Gustatory cortex External ear Middle ear Inner ear Auricle External auditory meatus Tympanic membrane Tympanic cavity Auditory ossicles Malleus Incus Stapes Tensor tympani Stapedius muscle Auditory tube Round window Oval window Otitis media Bony labyrinth Membranous labyrinth Semicircular canals Vestibule Cochlea Perilymph Endolymph Hair cells Ampulla Cupula Utricle Saccule Macula Otolith Vestibular duct Vestibular membrane Cochlear duct Tectorial membrane Basilar membrane Tympanic duct Organ of corti Auditory cortex Iris Pupil Lens Cornea Ciliary body Suspensory ligaments Sclera Choroid Retina Fovea Optic disc Neural retina Retinal epithelium Rod Cone Retinal ganglion cell Photopigments Glaucoma Cataract Visual cortex Anatomy 1 Lab Manual, Spring 2008 60 Lab 18: Endocrine System Lab Stations: 1. Identification: Torso model and worksheet 2. Game: Trivia 3. Histology: Endocrine tissue Lab Station 1: Identification: Torso model and worksheets Use the torso and brain models and your text to identify the following: Pituitary gland Thyroid gland Adrenal gland Kidney Heart Pancreas Gonads Pineal Gland Fill out the chart on the following two pages. I have filled out a few boxes for clarity. Answers will be available on ecompanion, try it first yourself. Lab Station 2: Game: Trivia Please use the cards provided to quiz each other. This is not a test; you are welcome to use your text and your lecture notes. Lab Station 3: Histology: Endocrine tissue Please look at the slides provided and make sure that you can identify: Thyroid gland: thyroid follicles, follicle cavity Parathyroid gland: principal cells Adrenal cortex: zona glomerulosa, zona fasciculata, zona reticularis Adrenal medulla Pancreas: islets of Langerhans, pancreatic exocrine cells (acini) Testes: interstitial cells Ovaries: follicle Pituitary: pars distalis, pars intermedia, pars nervosa Anatomy 1 Lab Manual, Spring 2008 Gland Hormone 61 From what cell/region Anterior Pituitary In response to Hypothalamus Hypothalamus Hypothalamus Hypothalamus Hypothalamus Hypothalamus Hypothalamus Posterior Pituitary Thyroid gland Parathyroid gland Pineal gland Thymus NA NA Action Anatomy 1 Lab Manual, Spring 2008 Gland Hormone 62 From what cell/region In response to Adrenal cortex Androgens Adrenal medulla Pancreas Testes Ovaries Pineal gland Zona reticularis NA Action Anatomy 1 Lab Manual, Spring 2008 63 TERMS FOR LECTURE 18: Hormone Negative feedback Releasing hormones Inhibiting hormones Pituitary gland Hypophysis Anterior pituitary Hypophyseal portal system Pars distalis Thyroid-stimulating hormone (TSH) Adrenocorticotropic hormone (ACTH) Follicle-stimulating hormone (FSH) Luteinizing hormone (LH) Prolactin Growth hormone (GH) Pituitary dwarfism Gigantism Acromegaly Pars intermedia Melanocyte-stimulating hormone (MSH) Posterior pituitary Pars nervosa Antidiuretic hormone (ADH) Oxytocin Thyroid gland Thyroid follicles Follicle cavity Thyroid hormone Goiter Hyperthyroid Hypothyroid Cretinism C cells Calcitonin Parathyroid gland Principal cells Parathyroid hormone Thymus Thymosin hormones Adrenal gland Adrenal cortex Corticosteroids Zona glomerulosa Mineralocorticoids Zona fasciculata Glucocorticoids Cortisol Zona reticularis Androgens Addison’s syndrome Adrenal medulla Chromaffin cells Pancreas Pancreatic islets Islets of Langerhans Alpha cells Glucagon Beta cells Insulin Type 1 diabetes Type 2 diabetes Testes Interstitial cells Testosterone Ovaries Oocytes Follicles Estrogen Corpus luteum Progesterone Pineal gland Pinealocytes Melatonin Anatomy 1 Lab Manual, Spring 2008 64 Lab 19: Blood and Heart Lab Stations: 1. Identification: Human heart and heart model 2. Dissection: Sheep heart Lab Station 1: Identification: Human heart and heart model Use the human heart, heart models, and your text to identify the following: Visceral pericardium Epicardium Myocardium Endocardium Atria Ventricles Interatrial septum Interventricular septum Inferior vena cava Superior vena cava Tricuspid valve Chordae tendineae Papillary muscle Trabeculae carneae Pulmonary trunk Pulmonary semilunar valve Pulmonary veins Bicuspid valve Aortic semilunar valve Ascending aorta Right coronary artery Left coronary artery Circumflex branch Great cardiac vein Middle cardiac vein Small cardiac vein Coronary sinus Anatomy 1 Lab Manual, Spring 2008 65 Lab Station 2: Dissection: Sheep heart 1. Divide yourselves into groups of 3-4. Get your gloves, a small dissecting tray, and a heart. 2. First orient yourself to the sheep heart: where is the base and the apex? Where is the coronary sulcus? Where are the ventricles and the atria? 3. Pick a ventricle and cut into the heart. Observe the chordae tendineae, papillary muscles, and the atrioventricular valve. Now cut into the other ventricle. Which ventricle is which? 4. Now use your finger to follow each ventricle backwards into an atrium, naming the valve through which you passed. Once in the atria, observe which veins bring blood to that atrium. 5. Follow the ventricles out through the semilunar valves so that you can identify the large arteries leaving the heart. Structures to identify on the sheep heart: Base Apex Right and left atria Right and left ventricles Pulmonary trunk Pulmonary veins Vena cava Aorta Chordae tendinae Papillary muscle Tricuspid valve Trabeculae carneae Pulmonary semilunar valve Bicuspid valve Aortic semilunar valve Note: Histology A microscope will be set up in the back of the class so that you can view a slide of blood. You should be able to see many erythrocytes and a few leukocytes. Anatomy 1 Lab Manual, Spring 2008 66 TERMS FOR LECTURE 19: Plasma Formed elements Erythrocytes Hemoglobin Anemia Polycythemia Leukocytes Lymphocyte Thrombocytes Megakaryocytes Myeloid tissue Pulmonary circulation Systemic circulation Arteries Veins Capillaries Pericardium Visceral layer Parietal layer Pericardial cavity Pericardial fluid Epicardium Myocardium Endocardium Atria Ventricles Interatrial septum Interventricular septum Inferior vena cava Superior vena cava Foramen ovale Atrioventricular valve Tricuspid valve Chordae tendinae Papillary muscle Trabeculae carneae Pulmonary trunk Pulmonary semilunar valve Pulmonary veins Mitral valve Bicuspid valve Aortic semilunar valve Ascending aorta Aortic arch Right coronary artery Left coronary artery Circumflex branch Great cardiac vein Middle cardiac vein Small cardiac vein Coronary sinus Angiogram Angina pectoris Myocytes Pacemaker cells Sinoatrial node Myocardial infarction Anatomy 1 Lab Manual, Spring 2008 67 Lab 20: Blood Vessels Lab Stations: 1. Identification: Vessels in the cadaver and models 2. Drawing: Vessels of the body Lab Station 1: Identification: Vessels in the cadaver and models Use the models and brain and cadaver to find all of the following blood vessels: Pulmonary trunk Pulmonary arteries Pulmonary veins Ascending aorta Aortic arch Brachiocephalic trunk Common carotid arteries Subclavian arteries Vertebral artery Axillary artery Brachial artery Deep brachial artery Radial artery Ulnar artery Internal carotid artery External carotid artery Anterior cerebral artery Middle cerebral artery Anterior communicating artery Basilar artery Posterior cerebral artery Posterior communicating artery Circle of Willis Descending aorta Thoracic aorta Abdominal aorta Common iliac arteries Internal iliac artery External iliac artery Femoral artery Popliteal artery Posterior tibial artery Anterior tibial artery Fibular artery Superior vena cava Inferior vena cava Confluence of the sinuses Internal jugular vein Brachiocephalic vein External jugular vein Subclavian vein Cephalic vein Median antebrachial vein Basilic vein Median cubital vein Axillary vein Radial vein Brachial vein Anterior tibial vein Posterior tibial vein Fibular vein Popliteal vein Femoral vein External iliac vein Common iliac vein Great saphenous vein Anatomy 1 Lab Manual, Spring 2008 68 Lab Station 2: Drawing: Vessels of the body Sketch the arteries and veins of the body. This does not have to be anatomically correct. You should become familiar with the branching patterns of the various vessels. You may sketch these out on your muscle drawings, or on a separate piece of paper. If you opt for the latter, you can sketch individual body areas out separately (as presented in lecture) or all together. Note: Histology There will be a microscope set up in the back of the lab room so that you can view a slide of an artery and vein. Make sure to become comfortable with the difference between the two. Anatomy 1 Lab Manual, Spring 2008 69 TERMS FOR LECTURE 20: Tunica externa Tunica media Tunica interna Vasoconstriction Vasodilation Elastic arteries Muscular arteries Arterioles Continuous capillaries Fenestrated capillaries Capillary bed Venule Medium-sized veins Large veins Valves Skeletal muscle pump Varicose veins Atherosclerosis Plaque Pulmonary trunk Pulmonary arteries Pulmonary veins Ascending aorta Aortic arch Brachiocephalic trunk Common carotid arteries Subclavian arteries Vertebral artery Axillary artery Brachial artery Radial artery Ulnar artery Internal carotid artery External carotid artery Anterior cerebral artery Middle cerebral artery Anterior communicating artery Basilar artery Posterior cerebral artery Posterior communicating artery Circle of Willis Descending aorta Thoracic aorta Abdominal aorta Common iliac arteries Internal iliac artery External iliac artery Femoral artery Popliteal artery Posterior tibial artery Anterior tibial artery Fibular artery Superior vena cava Inferior vena cava Confluence of the sinuses Internal jugular vein Brachiocephalic vein External jugular vein Subclavian vein Cephalic vein Median antebrachial vein Basilic vein Median cubital vein Axillary vein Radial vein Brachial vein Anterior tibial vein Posterior tibial vein Fibular vein Popliteal vein Femoral vein External iliac vein Common iliac vein Great saphenous vein Cerebrovascular accident Ischemic stroke Hemorrhagic stroke Anatomy 1 Lab Manual, Spring 2008 70 Lab 21: Lymphatic System Lab Activities: 1. Lymphatic Bingo 2. Dissection 3. Histology: Lymphatic system Lab Activity 1: Lymphatic Bingo The entire class will participate in lymphatic bingo. The winning table gets extra credit. Lab Activity 2: Cat Dissection We will expose the ventral body cavity of the cat in order to find the major organs and vessels. 1. To open the abdominal cavity: Use your scissors to make an incision midline near the groin. Cut upwards towards the diaphragm, but stop before reaching the diaphragm. Then cut along the underside of the ribs just under the diaphragm in each direction. From the initial groin incision, cut along the lower abdomen in both directions, so that you are left with two flaps that open the abdominal cavity. 2. To open the thoracic cavity: Use your scissors to cut along the axillary border of the chest on both sides, and then cut across the chest just above the diaphragm. Leave the diaphragm intact. Now you have a flap that opens the thoracic cavity. 3. In the abdominal cavity identify: small and large intestines, stomach, spleen, liver, and kidneys. Move these aside to identify: abdominal aorta, thoracic duct, inferior vena cava, and common iliac arteries and veins. 4. In the thoracic cavity identify: heart, lungs, thymus. Carefully dissect around the heart to find the aortic arch, brachiocephalic artery, left subclavian, right subclavian, and common carotid arteries. Please note that only two arteries branch off of the aortic arch, not three as in the human. Anatomy 1 Lab Manual, Spring 2008 Lab Activity 3: Histology: Lymphatic system Microscopes will be set up along the side of the classroom. Please make sure that you can identify each of the following and describe their function: Lymphocyte Lymph node: capsule, hilus, cortex, medulla Spleen: red pulp and white pulp Thymus: cortex, medulla 71 Anatomy 1 Lab Manual, Spring 2008 72 TERMS FOR LECTURE 21: Lymphatic capillaries Lymphatic vessels Lymphedema Superficial lymphatics Deep lymphatics Lymphatic trunks Lymphatic ducts Thoracic duct Cisterna chili Right lymphatic duct Lymph Specific immunity T cells Cell-mediated immunity Cytotoxic T cells Helper T cells Regulatory T cells B cells Humoral immunity Plasma cells Memory B cells AIDS HIV Lymphoid tissues Nodules Tonsils Tonsillitis Appendicitis Lymphoid organs Lymph nodes Hilus Lymphadenopathy Metastatic cancer Thymus Reticular cells Thymic hormones Spleen Red pulp White pulp Anatomy 1 Lab Manual, Spring 2008 73 Lab 22: Respiratory System Lab Stations: 1. Cardiopulmonary resuscitation 2. Identification: Human lung and models 3. Game: Trivia Lab Station 1: Cardiopulmonary Resuscitation Review the following cardiopulmonary resuscitation (CPR) instructions and answer the questions in bold. Please do not perform actual mouth to mouth on the doll provided. This is not meant to replace a traditional CPR course that you all should take. Rather, it is an exercise designed to allow you to understand the effects of CPR on the respiratory and cardiovascular systems. 1. Check the victim If the person is nonresponsive, tilt the head back and lift the chin. What does this do? Listen for breathing. If the person is not breathing, give 2 slow breaths to give them a little oxygen and to test whether the airway is blocked. Check for pulse at the side of the thyroid cartilage. Which artery are you feeling? 2. Rescue breathing If the person is not breathing but has a pulse, begin rescue breathing. Tilt the person’s head back, lift the chin, and pinch the nose shut. Why should you pinch the nose shut? Use specific terms from lecture. Give 1 slow breath every 5 seconds for 1 minute. Recheck pulse and breathing every minute. 3. CPR If the person is not breathing and has no pulse, initiate CPR. Kneel over the person and place the heel of your hand over the body of the sternum. Place your other hand on top of the first. Compress the chest 30 times. What does this do? Give 2 slow breaths. Why? Do 3 more sets of 30 compressions and 2 breaths. Recheck pulse and breathing. Continue until there is a pulse or until help arrives. 4. Heimlich maneuver If a person is choking, encourage them to cough. If the person can no longer breathe, give quick upward thrusts to the abdomen, just above the navel, until the airway is cleared. What does this do? (Be specific about mechanism) Anatomy 1 Lab Manual, Spring 2008 74 Lab Station 2: Identification: Human lung and models Use the models, your text, and the human lung to identify all of the following: Conducting zone Nasal cavity Pharynx Nasopharynx Oropharynx Laryngopharynx Glottis Larynx Thyroid cartilage Cricoid cartilage Epiglottis Trachea Tracheal cartilages Annular ligaments Hilus Cardiac impression Apex of the lung Base of the lung Left lobes Right lobes Parietal pleura Visceral pleura Bronchial tree Primary bronchi Secondary bronchi Tertiary bronchi Terminal bronchiole Alveoli Lobule Lab Station 3: Game: Trivia Please use the cards provided to quiz each other. This is not a test; you are welcome to use your text and your lecture notes. Note: Histology A microscope will be set up in the back of the lab room so that you can view a slide of alveoli. Anatomy 1 Lab Manual, Spring 2008 75 TERMS FOR LECTURE 22: Conducting zone Respiratory zone Goblet cells Mucus elevator Nasal cavity Paranasal sinuses Pharynx Nasopharynx Oropharynx Uvula Laryngopharynx Glottis Larynx Thyroid cartilage Laryngeal prominence Cricoid cartilage Epiglottis Vocal ligaments Vocal folds Trachea Tracheal cartilages Annular ligaments Trachealis muscle Tracheal blockage Tracheostomy Bronchi Bronchitis Hilus Lung Apex Base Lobes Pleura Parietal pleura Visceral pleura Pleural cavity Pleural fluid Pneumothorax Bronchial tree Primary bronchi Secondary bronchi Tertiary bronchi Terminal bronchioles Lobule Respiratory bronchioles Alveoli Type 1 cells Types 2 cells Inhalation Exhalation Respiratory centers Anatomy 1 Lab Manual, Spring 2008 76 Lab 23: Digestive System Lab Stations: 1. Identification: Cadaver and digestive models 2. Identification: Cat digestive system 3. Histology: Digestive tissue Lab Station 1: Identification: Cadaver and digestive models Please use the cadaver, models, and your text to find all of the following structures, and review the function of each: Oral cavity Pharynx Esophagus Stomach: rugae, cardia, fundus, body, pylorus, pyloric sphincter Greater omentum Lesser omentum Small intestine: plicae circulares, duodenum, jejunum, ileum, ileocecal valve, mesentery proper Large intestine: cecum, appendix, ascending colon, transverse colon, descending colon, sigmoid colon, rectum, mesocolon Liver: hepatocytes, sinusoids, lobule, hepatic triad, central vein, bile canaliculi Gallbladder Pancreas Lab Station 2: Identification: Cat digestive system One of the clearest cat dissections will be on display for you to identify the following digestive system structures: esophagus, stomach, small intestine, large intestine, liver, gallbladder, pancreas. Anatomy 1 Lab Manual, Spring 2008 77 Lab Station 3: Histology: Digestive tissue The goal of this station is to become comfortable with the histology of the digestive tract and accessory digestive organs. You will need to be familiar with general tissue characteristics of the entire digestive tract, as well as individual specializations of the various organs. For the digestive tract, please make sure to identify the following specializations. In each slide, also make sure to identify the common layers, listed below. Esophagus – stratified squamous epithelium, submucosa has esophageal glands Stomach – simple columnar epithelium, gastric pits Small intestine – simple columnar epithelium, intestinal villi, goblet cells, intestinal glands, lacteals Large intestine – simple columnar epithelium, no villi, goblet cells, lymphoid nodules, intestinal glands Common layers along the digestive tract: Mucosa – epithelium, areolar connective tissue Submucosa – areolar connective tissue Muscularis externa – circular and longitudinal smooth muscle layers Serosa – simple squamous epithelium, areolar connective tissue For the accessory digestive organs, make sure that you can identify: Liver: lobule, hepatocytes, central vein, hepatic triad Pancreas: islets of Langerhans (endocrine), pancreatic acini (exocrine) Anatomy 1 Lab Manual, Spring 2008 78 TERMS FOR LECTURE 23: Mucosa Muscularis mucosae Submucosa Muscularis externa Serosa Visceral peritoneum Parietal peritoneum Mesentery Oral cavity Salivary glands Mumps Pharynx Esophagus Stomach Rugae Chyme Pyloric sphincter Greater omentum Lesser omentum Gastric pits Gastric glands Gastric juice Parietal cells Instrinsic factor Chief cells Pepsinogen Ulcers Helicobacter pylori Small intestine Plicae circulares Duodenum Jejunum Ileum Ileocecal valve Mesentery proper Intestinal villi Brush border Intestinal glands Goblet cells Large intestine Cecum Appendix Ascending colon Transverse colon Descending colon Sigmoid colon Mesocolon Rectum Liver Bile Lobule Hepatocytes Sinusoids Kupffer cells Hepatic triads Bile canaliculi Central vein Gallbladder Common bile duct Sphincter of Oddi Gallstones Pancreas Pancreatic juice Anatomy 1 Lab Manual, Spring 2008 79 Lab 24: Urinary System Lab Stations: 1. Identification: Human kidney and models 2. Drawing: Nephron and pictionary 3. Histology: Nephron Lab Station 1: Identification: Kidney models Use the models provided to find the following: Renal capsule Cortex Medulla Renal pyramids Minor calyx Major calyx Renal pelvis Renal artery Segmental arteries Interlobar arteries Arcuate arteries Interlobular arteries Afferent arterioles Glomerulus Efferent arteriole Peritubular capillaries Venules Interlobular veins Arcuate veins Interlobar veins Renal vein Nephron Cortical nephron Juxtamedullary nephron Vasa recta Renal corpuscle Bowman’s capsule Parietal epithelium Visceral epithelium Capsular space Proximal convoluted tubule Descending limb of loop of Henle Ascending limb of loop of Henle Distal convoluted tubule Juxtaglomerular apparatus Collecting duct Ureter Bladder Lab Station 2: Drawing: Nephron and pictionary Use the paper and pens provided to draw a nephron. Include the blood vessels supplying the nephron (afferent and efferent arterioles, glomerular capillary bed, peritubular capillary bed). Label the sections and list the major function of each section. When you are finished, please use the Pictionary cards provided to practice drawing those structures. Make sure the review the function of each structure. Anatomy 1 Lab Manual, Spring 2008 Station 3: Histology : Nephron Use the slides provided of the kidney and ureter and make sure you can identify: Kidney: renal corpuscle, loop of Henle, proximal and distal convoluted tubule Ureter: transitional epithelium, lamina propria, smooth muscle, outer connective tissue 80 Anatomy 1 Lab Manual, Spring 2008 81 TERMS FOR LECTURE 24: Renal Renal capsule Cortex Medulla Renal pyramids Minor calyx Major calyx Renal pelvis Renal artery Segmental arteries Interlobar arteries Arcuate arteries Interlobular arteries Afferent arterioles Glomerulus Efferent arteriole Peritubular capillaries Vasa recta Venules Interlobular veins Arcuate veins Interlobar veins Renal vein Antidiuretic hormone Renin Nephron Cortical nephron Juxtamedullary nephron Renal corpuscle Glomerular capsule Parietal epithelium Visceral epithelium Capsular space Podocytes Filtrate Proximal convoluted tubule Descending limb of loop of Henle Ascending limb of loop of Henle Distal convoluted tubule Juxtaglomerular apparatus Collecting duct Kidney failure Ureter Bladder Rugae Internal urethral sphincter External urethral sphincter Urethra Urogenital diaphragm Micturation reflex Anatomy 1 Lab Manual, Spring 2008 82 Lab 25: Reproductive System Lab Stations: 1. Identification: Reproductive structures 2. Drawing: Gamete development 3. Histology: Reproductive tissue Lab Station 1: Identification: Reproductive structures Use the models and your text to find the following: Gonads Testis Seminiferous tubules Epididymis Ductus deferens Seminal vesicles Prostate gland Bulbourethral gland Penis Shaft Glans Prepuce Corpora cavernosa Corpora spongiosum Ovary Fallopian tubes Fimbriae Uterus Cervix Vagina Vestibule Labia minora Clitoris Labia majora Lab Station 2: Drawing: Gamete development Use the pens and paper provided to draw the steps involved in spermiogenesis and oogenesis. Make sure to follow a pair of chromosomes through meiosis in both processes, and show where meiosis is halted in the oocyte. For oogenesis, also draw in the follicular stages. Lab Station 3: Histology: Reproductive tissue Use the microscopes and slides provided to identify the following: Testes – seminiferous tubules, interstitial cells, sustentacular cells Penis – corpora cavernosa and corpora spongiosum, urethra Ovary – primary, secondary, and graafian follicles, corpus luteum Uterus – functional and basilar zones Anatomy 1 Lab Manual, Spring 2008 83 TERMS FOR LECTURE 25: Sperm Ovum Gamete Zygote Gonads Testes Seminiferous tubules Rete testis Epididymis Ductus deferens Interstitial cells Testosterone Sustentacular cells Spermatocyte Spermatozoa Spermatogenesis Acrosome Seminal vesicles Prostate gland Bulbourethral gland Semen Penis Shaft Glans Prepuce Preputial glands Smegma Corpora cavernosa Corpora spongiosum Ovary Follicles Oogenesis Oogonia Oocytes Graafian follicle Ovulation Corpus luteum Corpus albicans Menopause Fallopian tubes Fimbriae Uterus Cervix Cervical mucus Perimetrium Myometrium Endometrium Functional zone Basilar zone Menstrual cycle Vagina Vestibule Labia minora Clitoris Greater vestibular glands Labia majora Mammary glands Nipple Areola Lactiferous ducts Lactiferous sinus