Download CELL CENTER SERVICES

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UNIVERSITY OF PENNSYLVANIA
www.facebook.com/PennCellCenterServices
Location:
Tel:
Fax:
Website:
B14 Anatomy Chemistry Building
215/898-2795
215/573-9327
http://www.med.upenn.edu/cores/cell_center_services.shtml
Mailing Address:
B14 Anat./Chem. Building
3620 Hamilton Walk
Philadelphia, PA 19104
REQUEST FOR HYBRIDOMA GENERATION (FUSION)
User Name: __________________________________________ Penn Key_____________________________________
Principal Investigator: _________________________________ Fund No/ PO No: ______________________________
Date: _______________________________________________ Tel: __________________________________________
Email: ______________________________________________ Fax: _________________________________________
Prices subject to change. Contact Cell Center Services for current prices.
Antigen Information:
Antigen Name:______________________________________________
Is Antigen:
A Protein
A Peptide
Antigen concentration and amount: ____________________________
Antigen size: ____________________ kD
In case of Peptide: Is the peptide conjugated to a carrier protein? If yes, which carrier? _____________________________
Please check the requested service(s)
Peptide conjugation to KLH carrier protein
Immunization:
Fusion:
number of mice _____________ (the one mouse with the best titer will be used for fusion)
lymphocytes from one spleen will be plated into __________ x 96-well plates.
Fusion maintenance and harvesting of supernatants for testing
Screening of supernatants by ELISA
Expansion and freezing of positive clones:
Subcloning by limiting dilution:
number of clones _____________
number of clones ___________________
Staff Use Only
Start Date _______________________
Revised 04062017
Completed ______________________
By: _________________________