Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Control x y • How to get rid of the RHP zero? • What are the new tradeoffs? Bacterial cell Autocatalytic feedback Precursors Catabolism Environment cids Fatty a Co-factors Amin o Aci ds Genes Carriers Environment DNA replication Huge Variety Trans* Proteins Nutrients Core metabolism Huge Variety Catabolism Precursors Nutrients Taxis and transport Same 12 in all Core metabolism cells Acids o n i m A Nucleotides Fatty ac ids Carriers Same 8 in all cells Gly G1P G6P F6P Reaction F1-6BP Err/flow ctrl Gly3p Mgmt ATP 13BPG 3PG 2PG NADH Oxa PEP Pyr ACA TCA Cit Gly G1P G6P F6P F1-6BP Gly3p ATP How does all this feedback work? 13BPG 3PG 2PG NADH Oxa PEP Pyr ACA TCA Cit Gly G1P G6P F6P F1-6BP Gly3p ATP ATP 13BPG 3PG 2PG NADH Oxa PEP Pyr ACA TCA Cit Autocatalytic x Control y F6P F1-6BP Gly3p 13BPG 3PG ATP Autocatalytic x Control y Autocatalytic Control [ATP] 1.05 1 h >> 1 0.95 Time response 0.9 Yet fragile 0.85 h=1 0.8 15 20 e r o Time (minutes) m s e r a c e r u t a N . f f o Note: e h >>1 d a r t s i h t t u Spectrum abo 0 5 10 0.8 Robust Log(Sn/S0) 0.6 0.4 0.2 h=1 0 -0.2 -0.4 -0.6 -0.8 0 2 4 Frequency 6 8 10 output=x C + Plant Small z is bad. Small z is bad (oscillations and crashes) Small z = • small k and/or • large q Efficiency = • small k and/or • large q Correctly predicts conditions with “glycolytic oscillations” x rate y level More enzyme Autocatalytic x Control y q consumed produced Autocatalytic x Control y Layered control rate form/activity level Autocatalytic x Control y Enzyme complexity Autocatalytic x Control y Autocatalytic x Control y Small z = • small k and/or • large q Efficiency = • small k and/or • large q Autocatalytic x Control y • How to get rid of the RHP zero? • What are the new tradeoffs? Control x y • How to get rid of the RHP zero? • What are the new tradeoffs? Control x y • How to get rid of the RHP zero? • What are the new tradeoffs? More control x x y y More complex enzymes