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CHAPTER 3 COMPREHENSIVE LAB HEREDITY, PUNNETT SQUARES, & PEDIGREES From CELLS, HEREDITY, & CLASSIFICATION NAME ___________________________ PERIOD _______ Imagine that you are a designer for a toy company that makes toy alien bugs. The president of Bug Builders Inc., wants new versions of the wildly popular Space Bugs, but he wants to use the bug parts that are already in the warehouse. It’s your job to come up with a new bug design. You have studied how traits are passed from one generation to another. You will use this knowledge to come up with new combinations of traits and assemble the bug parts in new ways. Model A and Model B will act as the “parent” bugs. The two models currently available are shown on page 76 of your textbook. Materials: 16 Allele sacks (supplied by your teacher) Large marshmallows (head and body segments) Red and green toothpicks (antennae) Other colors of toothpicks (to attach body parts together and for legs) Green and blue thumbtacks (noses) Pipe cleaners (tails) Green and red gumdrops (feet) Googly eyes Scissors Glue Flowers (girl bugs only) You will work with a partner for this project. You and your partner will be creating ONE new bug design. The other pairs in your class will be creating designs of their own. Remember – even though you will be working with a partner, you must each complete your own lab paper and will be graded on the paper that you submit, not your partner’s paper. We will be working on this lab for a few days, so make sure you do not lose your paper! You will not get to choose which traits your bug has – we will follow Mendel’s Laws of Heredity, which dictate that genes and traits are passed down randomly from the parents to the offspring (aka the Law of Independent Assortment). We will also follow Mendel’s Law of Segregation, which tells us that each parent will donate one allele, or set of instructions, for each bug trait. This means that you will randomly choose one allele from the “mom” bug (printed on red paper) and one allele from the “dad” bug (printed on blue paper). This process will simulate that production of the sex cells in the parent bugs and the subsequent passing on of those sex cells to the offspring. When you have collected all of the alleles you need for your baby bug, you will match up each homologous pair of alleles, simulating fertilization. This packet contains tables for you to record your data and questions that you need to answer. Make sure you fill out all tables and answer all questions before you turn in your packet! First… Before you begin building your baby bug we want to know the possible genotypes of the parent bugs. You can see the phenotypes of the parent bugs by looking at the models or by using the information on page 76 of your textbook. You will also need the information on page 77 to fill in the table below. Possible Genotypes of the Parents “MOM” BUG TRAIT PHENOTYPE POSSIBLE GENOTYPE(S) Antennae Color # of Body Segments Tail Shape # of Leg Pairs Nose Color Foot Color # of Eyes Gender “DAD” BUG TRAIT Antennae Color # of Body Segments Tail Shape # of Leg Pairs Nose Color Foot Color # of Eyes Gender PHENOTYPE POSSIBLE GENOTYPE(S) Procedure: 1. Your teacher will pass around 16 allele sacks. These contain the alleles that the mom and dad bug could possibly carry according to their specific traits. The mom and dad bug are not true-breeding for all of their traits, so there are many different combinations possible for each baby bug. 2. Choose one allele card from each sack (no peeking!), then pass the sack on to the next group. 3. Lay all 16 alleles out on your table and match up the pairs of letters. 4. Record the alleles your baby bug has received, the genotype, and the phenotype of your baby bug in the table below. When writing the genotype, remember that the dominant allele should be written first. Be sure to use the correct letter for each trait. You can find out which letters to use and which alleles are dominant and recessive by looking at the table on page 77 of your textbook. TRAIT “MOM” ALLELE “DAD” ALLELE BABY GENOTYPE BABY PHENOTYPE Antennae Color # of Body Segments Tail Shape # of Leg Pairs Nose Color Foot Color # of Eyes Gender 5. After you have filled out the table above, carefully put your allele cards back in the sacks. Please make sure you put each allele in the correct sack so they will not be mixed up for future classes! 6. Now that you know what your baby bug will look like, you are ready to build it! Using your table as a guide (look at the “phenotype” column), carefully choose the items you need to build your bug from the supplies on the front table. Here are some tips to remember: a. Get enough marshmallows for the # of body segments plus one for the head (and no, you may not eat them!). b. Use the green and red toothpicks for antennae ONLY – use other colors for the legs and to attach the body segments and head together. c. You may use glue to help hold the body together if necessary and to attach the eyes. d. Break or cut the toothpicks in half to make the antennae – this means you will only need ONE red or green toothpick. Analyze the Results: 7. Take a class poll of the traits of the offspring by putting a tally mark next to your baby bug’s traits in the table on the SmartBoard. Record the results of the entire class in the table below. What are the ratios of each trait? Traits of the Offspring (class totals) DOMINANT TRAIT # BABIES EXHIBITING DOMINANT TRAIT RECESSIVE TRAIT Red Antennae Green Antennae 3 Body Segments Curly Tail 2 Body Segments Straight Tail 3 Leg Pairs 2 Leg Pairs Blue Nose Green Nose Green Feet Red Feet 2 Eyes 3 Eyes # BABIES EXHIBITING RECESSIVE TRAIT Gender Boy Babies Girl Babies RATIO OF DOMINANT TO RECESSIVE TRAITS Draw Conclusions: 8. Do any of the baby bugs look exactly like their parents? ______________ Explain your findings. __________________________________________________________________ _________________________________________________________________________ 9. Was this an example of selective breeding? ________________________ Explain your answer. ______________________________________________________________ _________________________________________________________________________ Going Further: 10. Your little baby bug has grown up quickly. It is now time for him/her to find a mate and have a baby of their own! Remember that you need to find a mate of the correct gender so your baby bug can successfully reproduce. Depending on the number of male and female bugs in your class this may mean that some bugs have to “mate” with more than one other bug. If this happens to your bug you only need to record the results for one of his/her partners. Record the data for your bug, your bug’s partner, and the possible genotypes and phenotypes of their offspring in the table below. * You will need to create Punnett Squares for each trait in order to determine the possible genotypes and phenotypes! * TRAIT OUR BUG Genotype Antennae Color # of Body Segments Tail Shape # of Leg Pairs Nose Color Foot Color # of Eyes Gender Phenotype PARTNER BUG Genotype Phenotype POSSIBLE GENOTYPES OF OFFSPRING POSSIBLE PHENOTYPES OF OFFSPRING 11. Now you need to draw a pedigree for the bug family that your class has created. You are going to make a pedigree for the trait of antennae color. Some information you will need to know before you draw your pedigree is: a. What color antennae do the original “mom” and “dad” bugs have? (First Generation) b. How many babies did the original “mom” and “dad” bugs have? (2nd Generation) c. How many of the second generation bugs were boys? Girls? d. Which color antennae does your baby bug have, and which color did his/her partner have? e. What possible genotypes/phenotypes for antennae color could your baby and his/her partner’s children have? f. Assume your baby and his/her partner had 4 children. Include these 4 children on the pedigree. (3rd Generation) Draw your pedigree in the space below: Review Questions: 1. What is an “allele”? ______________________________________________________ ______________________________________________________________________ 2. What does “true-breeding” mean? ___________________________________________ ______________________________________________________________________ 3. What does “hybrid” mean? ________________________________________________ ______________________________________________________________________ 4. What process was simulated when you randomly picked alleles from the mom and dad and matched them with one another? ________________________________________ 5. What are the matching pairs of chromosomes in your new baby called? ______________________________________________________________________ 6. What was the only sex chromosome that the mom bug could pass on? ______________ 7. Which sex chromosome did the dad bug pass on to your baby bug? ________________ 8. Is your baby bug a boy or a girl? _________ How do you know? __________________ ______________________________________________________________________ 9. What is a “genotype”? ____________________________________________________ ______________________________________________________________________ 10. What is a “phenotype”? ___________________________________________________ ______________________________________________________________________ 11. What type of letter represents a dominant allele? ______________________________ 12. What type of letter represents a recessive allele? ______________________________ 13. What is a Punnett Square? ________________________________________________ ______________________________________________________________________ 14. What is a Pedigree? _____________________________________________________ ______________________________________________________________________