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FP6 PROJECT “NEUROIMAGE”
SEE Regional Database
CLIENT FORMS
Dissemination Workshop NEUROIMAGE
School of Biology, University of Belgrade
22.-24. 09. 2007.
Belgrade
FP6 PROJECT “NEUROIMAGE”
SEE Regional Database
CONTENTS:
1.
LNMCP – Laboratory of Neuroendocrinology/Molecular Cell Physiology,
Institute of Pathophysiology, Faculty of Medicine, University of Ljubljana
(Ljubljana, Slovenia)
2.
INCNR – Institute of Neuroscience CNR - (Pisa, Italy)
3.
LMN – Laboratory for Molecular Neuroscience, Center for Molecular Biology
and Neuroscience, University of Oslo (Oslo, Norway)
4.
CRLM – “Rita Levi-Montalcini” Centre for Brain Repair (Turin, Italy)
5.
CIBR – Croatian Institute for Brain Research (Zagreb, Croatia)
6.
BIP – Cytology Department, Bogomoletz Institute of Physiology, National
Academy of Sciences of Ukraine (Kiev, Ukraine)
7.
IBR – Institute for Biological Research, University of Belgrade (Belgrade,
Serbia)
8.
INEP – Institute for Application of Nuclear Energy, University of Belgrade
(Belgrade, Serbia)
FP6 PROJECT “NEUROIMAGE”
SEE Regional Datbase
1. LNMCP
Research Team
• Research Team Name:
Center LNMCP - Medical Faculty, University of Ljubljana, Institute of PathophysiologyLaboratory of Neuroendocrinology - Molecular Cell Physiology
• Research Team Leader Name:
Prof. Dr. Robert Zorec
Research Team Members
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Robert Zorec
Marko Kreft
Helena Chowdhury
Matjaž Stenovec
Nina Vardjan
Sonja Grilc
Maja Potokar
Mateja Gabrijel
Jernej Jorgačevski
Mateja Prebil
Brina Kovačič
Research Team Projects
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Project Name: Cell Physiology
Project Leader: Robert Zorec
Project Funding Agency: Slovenian research agency
Project Budget (optional): 300 000/year
Project Start Date: 1.1.2004
Project End Date: 31.12.2008
Project Partners: Celica d.o.o.
• Project Summary:
Chemical messengers and hormones are stored in cells in membrane bound vesicles. The
contents of vesicles is released into the extracellular medium upon the fusion of the
vesicle membrane with the plasma membrane, a process termed exocytosis. In many cells
a stimulus is required to trigger exocytosis (regulated exocytosis), while in others
exocytosis proceeds in a continuous fashion (constitutive exocytosis).
Practically all cells in human body perform a form of exocytosis. In some cells, such as
neurons and endocrine cells, this process is particularly specialized. However, it is also
present in adipocytes, cardiomiocytes, immune cells, photoreceptors, glial cells, plant
cells and other cell types. Although exocytosis is an ubiquitous process of all eukaryotic
cells, the molecular mechanisms regulating it are still unclear. In the last decade many
proteins have been discovered to play a role in exocytosis, but the exact order and nature
of their interactions underlying regulated exocytosis is unclear. On one side it has been
hypothesized that a common molecular mechanism is essential for regulated exocytosis,
but the interaction of other molecules with the essential set of molecules contribute to
specific functional requirements. On the other side it has been proposed that regulated
exocytosis is explained by a sequential molecular model, which states that all vesicles in
a cell must undergo a sequence of events in order to fuse with plasma membrane.
To test these hypotheses, we study secretory activity in a number of model cell types,
especially in pituitary cells from the anterior and intermediate lobes, which secrete
prolactin, beta-endorphin and alpha-melanocyte stimulating hormone. These hormones
are involved in the bodily response to stress, regulate the immune system, body
temperature, body weight and other functions. In adition to this we also study the
physiology of mitochondria, secretory activity of single astrocytes, adipocytes, skeletal
muscle fibres and other cells.
Membrane fusion is an important process also in cell maturation, such as in the formation
of skeletal muscle fibres. It also represents a key step in the production of hybrid cells
used in the production of monoclonal antibodies and for the preparation of hybrid cells in
immunotherapy of cancer. In these applications membranes of two adjacent cells fuse to
form one cell-hybrid. In case of immunotherapy the fusion of dendritic cells with tumor
cells enables the hybrid to retain properties of antigen presenting cells, but is presenting
tumour antigens to other immune cells at the same time.
The aim of the "Cell Physiology" programme is therefore on one side to understand the
fundamental properties of membrane fusion under physiological and pathological
conditions, and on the other side to utilize this knowledge in developing hybrid cells and
cell therapy related products.
• Project Website:
http://www2.mf.uni-lj.si/~neuroendo/
Contact Person
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Name: Robert Zorec
Email: [email protected]
Function: PI, professor
Phone: + 386 1 546 7020
Fax: + 386 1 546 7020
Website: http://www2.mf.uni-lj.si/~neuroendo/
Research Team Objectives and Resources (list all relevant, but special emphasize
should be made to those related/complementary to NEUROIMAGE)
• Main Fields:
Electrophysiological techniques of patch-clamping (membrane capacitance), fluorescence
(photometric and imaging) methods to measure changes in cytosolic calcium, caged
calcium photolysis, confocal microscopy to evaluate processes in bioengineering,
tracking of particles, measuring fluorescence of pH reporters.
• Partners/Interests:
The Laboratory of Neuroendocrinology - Molecular Cell Physiology (LN-MCP) is part of
the Institute of Pathophysiology, Faculty of Medicine, University of Ljubljana, Slovenia.
The program of the LN-MCP, is to study molecular mechanisms of regulated exocytotic
hormone secretion. The function of a single cell is studied by electrophysiological
techniques of patch-clamping, where the secretory activity is monitored by changes in
membrane capacitance. Since cytosolic calcium activity is the trigger of exocytosis in
excitable cells, fluorescence (photometric and imaging) methods are used to measure
changes in cytosolic calcium. Caged calcium photolysis is used to produce a step change
in cytosolic calcium. A method was developed to quantify the interaction between a
single secretory granule and plasmalemma, detected as discrete steps in membrane
capacitance (femtofarad steps). Confocal microscopy is used to study molecular
mechanisms of regulated exocytosis in endocrine cells, astrocytes, muscle cells and
photoreceptors and to track intracellular compartments. We also use confocal microscopy
to evaluate processes in bioengineering.
• Main resources - instrumentation:
Fully equipped laboratories for biochemistry, molecular biology, immunofluorescence,
clean rooms for cell culture, electrophysiology (patch-clamp, amperometry) and
fluorimetry (monochromators, sensitive cameras for particle tracking, FRET and Ca2+imaging); two confocal microscopes including spectral detector, image analysis.
Institution/University
• Name of Institution/University: University of Ljubljana
• Number of Employees: 5789
• Number of Researchers: 3013
• Profit / Non-profit
• Country: Slovenia
• Postal Address: Kongresni trg 12, 1000 Ljubljana
________________________
FP6 PROJECT “NEUROIMAGE”
SEE Regional Datbase
CLIENT FORM
2. INCNR
Research Team
• Research Team Name:
• Research Team Leader Name: Gian Michele Ratto
Research Team Members
1. Mario Costa
2. Matilde Marchi
3. Riccardo Parra
4. Marco Basso
5. Elisa Brilli
…
Research Team Projects
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Project Name: Live cell imaging of the molecular dynamics of the ERK pathway
Project Leader: G. M. Ratto
Project Funding Agency: CNR, SNS, International Foundation for Paraplegia
Project Budget (optional):
• Project Start Date: 2005
• Project End Date: 2009
• Project Partners:
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Project Name: Live cell imaging of molecular models of Rett’s syndrome
Project Leader: M. Costa
Project Funding Agency: CNR
Project Budget (optional):
Project Start Date: 2007
Project End Date: 2010
Project Partners:
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Project Name: Two photon imaging of the epileptic brain
Project Leader: G. M. Ratto
Project Funding Agency: CNR, SNS, Telethon.
Project Budget (optional):
Project Start Date: 2007
Project End Date: 2010
Project Partners:
Project Summary:
Project Website:
Contact Person
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Name: Gian Michele Ratto
Email: [email protected]
Function: Senior researcher
Phone: +39 050 315 3168
Fax: +39 050 315 3220
Website:
Research Team Objectives and Resources (list all relevant, but special emphasize
should be made to those related/complementary to NEUROIMAGE)
• Main Fields: Molecular, cellular and structural mechanisms of plasticity. Two photon
in vivo imaging. Imaging of molecular dynamics.
• Partners/Interests:
• Main resources - instrumentation: 3 visible light confocal microscopes. Two photon
confocal microscope. Set ups for patch clamp and field recording.
Institution/University
• Name of Institution/University: NEST, Istituto Nazionale di Fisica della materia/CNR
and Scuola Normale Superiore.
• Number of Employees: >1000
• Number of Researchers: >1000
• Non-profit
• Country: Italy
• Postal Address: Piazza dei Cavalieri, 7- I-56126 Pisa (Italy)
________________________
FP6 PROJECT “NEUROIMAGE”
SEE Regional Datbase
3. LMN
Research Team
• Research Team Name: Lab for Molecular Neuroscience
• Research Team Leader Name: Ole Petter Ottersen
Research Team Members
1. Ole Petter Ottersen
2. M Amiry-Moghaddam
3. E. A. Nagelhus
4. Gabriele Nase
5. plus several others
…
Research Team Projects
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Project Name: Mechanisms for ischemic cell death and edema in the CNS
Project Leader: Ole Petter Ottersen
Project Funding Agency: EU, Nordic Council, Norwegian Research Council etc
Project Budget (optional):
Project Start Date: running
Project End Date:
• Project Partners: Several laboratories in Europe, Japan, and USA
• Project Summary: see www.cmbn.no
• Project Website: www.cmbn.no
Contact Person
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Name: Ole Petter Ottersen
Email: [email protected]
Function: Director, professor
Phone: + 47 22 85 12 70
Fax:+ 47 22 85 14 88
Website: www.cmbn.no
Research Team Objectives and Resources (list all relevant, but special emphasize
should be made to those related/complementary to NEUROIMAGE)
• Main Fields: synaptic transmission, excitotoxicity, stroke, water transport and brain
edema
• Partners/Interests:
• Main resources - instrumentation: Electron microscopes, in vivo optical imaging by
multiphoton laser scanning microscopy
Institution/University
• Name of Institution/University: Centre for Molecular Biology and Neuroscience
(CMBN), University of Oslo
• Number of Employees: 108 employees in CMBN
• Number of Researchers: 80 in CMBN
• Profit / Non-profit: Non profit
• Country: Norway
• Postal Address: PO Box 1105, 0317 Oslo
________________________
FP6 PROJECT “NEUROIMAGE”
SEE Regional Datbase
4. CRLM
Research Team
• Research Team Name: Laboratory of Neurobiology
• Research Team Leader Name:
Ferdinando Rossi
Research Team Members
Annalisa Buffo
Daniela Carulli
Assistant Professor
Assistant Professor
Sara Gianola
Postdoc
Ian Martin Williams
Ketty Leto
Alice Bartolini
Annarita De Luca
PhD student
PhD student
PhD student
PhD student
Luisella Milano
Francesco Bertolo
Technician
Technicia (Veterinary)
Research Team Projects
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Project Name:
Project Leader:
Project Funding Agency:
Project Budget (optional):
Project Start Date:
Project End Date:
Project Partners:
• Project Summary:
• Project Website:
<Repeat for additional projects>
Contact Person
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Name: Ferdinando Rossi
Email: [email protected]
Function: PI
Phone: +39 011 670 8165
Fax: +39 011 670 7708
Website: http://www.personalweb.unito.it/ferdinando.rossi/
Research Team Objectives and Resources (list all relevant, but special emphasize
should be made to those related/complementary to NEUROIMAGE)
• Main Fields:
• Role of oligodendrocyte- and myelin-associated molecules in the regulation of
Purkinje axon regeneration and plasticity during development and in the adult
• Interaction between neuronal growth-associated genes and environmental inhibitory
factors during axon regeneration and plasticity in the mammalian CNS
• Neuronal response to axotomy: interplay between the growth gene expression,
neuronal survival and axon regeneration
• Mechanisms of specification of stem and progenitor cells during neural development
and repair
• Differentiation and integration of transplanted cells into the adult CNS
• Intrinsic neurogenic potential and repair in the adult cerebellum
• Partners/Interests:
• Prof. Martin E. Schwab, Brain Research Institute, University of Zurich (Switzerland)
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Dr. Joost Verhaagen, Brain Research Institute, Amsterdam (The Netherlands)
Dr. Richard Hawkes, University of Calgary, Alberta (Canada)
Dr Marion Wassef, Ecole Normale Superieure, Paris (France)
Prof. Karl Schilling,University of Bonn (Germany)
Prof. Elena Cattaneo, University of Milan (Italy)
Dr Lorenzo Magrassi, University of Pavia (Italy)
Prof Gianfranco Gennarini, University of Bari (Italy)
Dr Giacomo Consalez, DiBit HSR, Milan (Italy)
Associate Member of the NeuroNE, European Consortium for Research on
Neurodegenerative Diseases
• Main resources - instrumentation:
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Animal house for breeding and housing, facility for rodent surgery (surgical
microscope, WPI PV800 pneumatic picopump, Midgard CS4 iontophoretic
device)
Hystology and molecular biology laboratory (cryostat, vibratome, chemical
hoods, incubation ovens, RT-PCR, real time PCR, centrifuges, etc.)
Cell culture laboratory (horizontal and vertical laminar flow hoods, incubators,
centrifuges, deep freezers, microscope, etc.)
Light microscopy facility (Zeiss Axiophot light micorscope, bright field,
nomarski optics, fluorescence, with digital camera Nikon Coolpix 950; Nikon E800 light microscope, bright field, fluorescence, with CCD camera and
Neurolucida image-morphometric analysis system)
Fluoview FV300 confocal microscope equipped with Coherent lasers for dual
photon microscopy
Institution/University
• Name of Institution/University:
University of Turin, Department of Neuroscience
• Number of Employees:
150
• Number of Researchers:
120
• Profit / Non-profit
Academia
• Country:
Italy
• Postal Address:
Corso Raffaello 30
I-10125 Turin
ITALY
________________________
FP6 PROJECT “NEUROIMAGE”
SEE Regional Database
5. CIBR
Research Team
• Research Team Name: Section for Neurogenetics, Cytogenetics and
Developmental Genetics
• Individual Researcher: Prof. dr. Srećko Gajović
• Research Team Leader Name: Prof. dr. Srećko Gajović
Research Team Members
1. dr. Dinko Mitrečić
2. ing. Sandra Mavrić
Research Team Projects
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Project Name: Gene function in differentiation and plasticity o mouse central nervous system
Project Leader: Prof. dr. Srećko Gajović
Project Funding Agency: Ministry for Science, Sport and Education
Project Budget: 12000 EUR/year
Project Start Date: January 2007
Project End Date: December 2011
Project Partners: School of Medicine, University of Osijek; ULB, Belgium; ICGEB,
Italy; University of Hannover, Germany
• Project Summary:
Insight in mammalian gene function in vivo will be obtained through phenotype analysis
of genetically modified mice. Molecular basis of differentiation and plasticity of the
central nervous system will be investigated on 6 mouse mutants. Pax3 (Paired box gene
3) function will be assesed in splotch mice, which exhibit neural tube defects and spina
bifida. Noto (Notochord homologue) function will be asessed in truncate (spontaneous
mutation) and NotoGFP mice (knockout), which exhibit partial lack of notochord and
disturbed differentiation of the neural tube. Stam2 (signal transducing adaptor molecule
2), Nol1 (nucleolar protein 1) and Klf8 (Krueppel like transcription factor 8) function will
be assessed on mice obtained by gene trap method. This method involves random
insertion of DNA vector, which monitors the expression of the mutated gene with lacZ as
a marker, and in addition disturbes the production of the corresponding protein. Stam2 is
involved in cell signaling through endocytic pathway, Nol1 is involved in ribosome
biogenesis in nucleolus, and Klf8 is a transcription factor with unknown function related
to mental retardation.
In order to elucidate gene function in differentiation and plasticity of the central nervous
system, Pax3 and Noto activity will be revealed in neuroectoderm differentiation through
neurulation, during the closure of the posterior neuropore and in the pathogenesis of spina
bifida. Stam2 function will be investigated in the neurons in relation to cell signaling
connected with endosomal transport of cargo and membranes during differentiation and
plasticity in the brain.
Molecular characterization of the mouse mutants and expression pattern determination of
the involved genes will serve as a prerequiste for the phenotype analysis. The central
nervous system phenotype of developing and adult mutant mice will give insight in gene
function in vivo. This approach will be complemented with experiments in vitro, which
should disclose the molecular and cellular basis of the observed changes. This will reveal
not only the consequences of genetic change on the system level, but as well on the
cellular level. Therefore the implications of the proposed reasearch cover genetic basis of
human brain diseases, but as well basic understanding of the gene activity and regulation
during differentiation and plasticity in the central nervous system.
• Project Website: http://neurogenetika.hiim.hr
Contact Person
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Name: Prof. dr. Srećko Gajović
Email: [email protected]
Function: Head of Section
Phone: +385 1 4596829
Fax: +385 1 4596942
Website: http://neurogenetika.hiim.hr
Research Team Objectives and Resources
• Main Fields: neurogenetics, mouse molecular genetics, mouse embryology
• Partners/Interests: confocal microscopy, electron microscopy
• Main resources - instrumentation: Zeiss LSM 510 Meta, Transmission electron
microscope Zeiss 902A
Institution/University
• Name of Institution/University: School of Medicine University of Zagreb
• Number of Employees: 600
• Number of Researchers: 400
• Country: Croatia
• Postal Address: Šalata 3, HR-10000 Zagreb
________________________
FP6 PROJECT “NEUROIMAGE”
SEE Regional Datbase
CLIENT FORM
6. BIP
Research Team
• Research Team Name: Cytology Department, Bogomoletz Institute of Physiology,
NAS of Ukraine
• Research Team Leader Name: prof. Skibo G.G.
Research Team Members
1. prof. Skibo Galina (DrSci, PhD, M.D.)
2. Orlovsky Maxim (PhD, MD)
3. Kovalenko Tatyana (Phd)
4. Osadchenko Irina (Phd)
5. Tsupikov Oleg (PhD fellow)
6. Lebed Yury (PhD fellow, MD)
…
Research Team Projects
• Project Name: Neuronal and astroglial hippocampal changes in the early stages of type
I diabetes mellitus in rats
• Project Leader: Skibo G.G.
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Project Funding Agency: National Academy of Sciences of Ukraine
Project Budget (optional):
Project Start Date: 13.06.06
Project End Date: 25.05.08
Project Partners: Department of General Physiology of Nervous System (head
P.G.Kostyuk), Voitenko research group (Nana V. Voitenko, DrSci, PhD); Department
of Experimental Cardiology (head A.A.Moibenko), Viktor Dosenko (DrSci, PhD,
M.D.).
• Project Summary: Cognitive impairments is a well-known complication of type 1
diabetes mellitus (DM) which is related to hippocampus injury. Present investigation
was undertaken to study hippocampal changes in early stages of diabetes
development. DM was induced by single streptozotocin injection in male rats. Rats
were decapitated on day 3, 7 and 14 and perfused with 4% paraformaldehyde.
Vibratome sections (70 µm) were used for further simultaineous detection of NeuN
(specific neuronal protein), GFAP (intermediate filament protein, that is synthesized
inside astrocytes only) and Hoechst 33324 binding (DNA fluorochrome). We
employed fluorescence-labeled goat anti-mouse (Alexa Fluor 488) and goat anti-rabbit
(Alexa Fluor 568) IgG to detect neuronal and astroglial markers. Confocal imaging
system "Olympus FV1000" equipted with multi-line argon laser (457/477, 488/ 515
nm, 40 mW), green helium neon laser (543 nm, 1 mW), and UV-argon laser
(351/356nm 50 mW) was used for specimen visualization and analysis. This approach
gave possibility to assess spatial interrelations between neurons and astrocites, nuclear
and cellular morphology as well as quantify neuronal and glial cells. Found changes
suggested intensive neuronal loss and astrogliosis already during early stages of
diabetes development.
• Project Website:
<Repeat for additional projects>
Contact Person
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Name: Yury
Email: [email protected]
Function: PhD fellow
Phone: +380 44 256-24-43
Fax:
+380 44 256-24-58
Website: http://www.biph.kiev.ua/departments/cytol/index.html
Research Team Objectives and Resources (list all relevant, but special emphasize
should be made to those related/complementary to NEUROIMAGE)
• Main Fields: Studying of brain disorders during type I diabetes mellitus development
in rats; investigations of ischemic brain injury in vitro and in vivo.
• Partners/Interests: Department of General Physiology of Nervous System (head
P.G.Kostyuk), Voitenko research group (Nana V. Voitenko, DrSci, PhD); Department
of Experimental Cardiology (head A.A.Moibenko), Viktor Dosenko (DrSci, PhD,
M.D.).
• Main resources - instrumentation:
1. Confocal microscope FV1000-BX61WI (Olympus, Japan)
a)Lasers:
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Multi-line Argon laser (457/ (477), 488/ 515 nm (40 mW))
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Green Helium Neon laser (543 nm (1 mW))
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UV-Argon laser (351/356nm (50 mW))
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UV 405 nm laser diode system
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Laser diode system 440 nm
b)Software:
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FluoView
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IMARIS (full package)
c)Optics:
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Up-right frame
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DIC optics
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Objectives:
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UPLFL10x/0.30
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UPLFL 20x/0.50
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UPLAPO40x/0.85
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PLAPO60x0.3/1.4
Institution/University
• Name of Institution/University: Bogomoletz Institute of Physiology
• Number of Employees: 14
• Number of Researchers: 9
• Profit / Non-profit – Non-profit
• Country: Ukraine
• Postal Address: 01024 Kiev, Bogomoletz street, 4.
________________________
FP6 PROJECT “NEUROIMAGE”
SEE Regional Datbase
CLIENT FORM
7. IBR
Research Team
• Research Team Name: Brain plasticity team
• Research Team Leader Name: Dr Selma Kanazir
Research Team Members
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Milka Perovic
Aleksandra Mladenovic
Desanka Milanovic
Vesna Pesic
Kosara Smiljanic
Natasa Loncarevic
Jelena Popic
Research Team Projects
• Project Name: MOLECULAR AND BEHAVIORAL STUDY OF BRAIN
PLASTICITY
• Project Leader: Dr Selma Kanazir
• Project Funding Agency: Ministry for Science, Republic of Serbia
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Project Budget (optional): ca. 20 000 EUR
Project Start Date: 2006
Project End Date: 2010
Project Partners: none
• Project Summary:
Brain plasticity is specific quality of the nervous system to react and adjust to the internal
and external environmental changes, both in physiological and pathological conditions.
Brain plasticity is greatly compromised during aging and stress, but some environmental
factors, like dietary manipulations could attenuate or prevent some age- and stress-related
changes. In this project we have proposed to study the effects of long-term food restriction
(FR) on various aspects of rat brain plasticity in normal-physiological aging and in
response to stress. We are examining the expression pattern of genes related to a) synaptic
plasticity (synaptophysin, GAP-43, and N-cadherin), and b) neurodegenerative changes
(alfa-synuclein, amyloid precursor protein (APP) and proteins involved in APP
metabolism and cholesterol metabolism), in the cortex, hippocampus and cerebellum. We
are determining the effects of aging, stress and food restriction on the expression profile of
these genes are as well as the correlation between changes in proteins related to
neurodegeneration and synaptic plasticity. Finally, we are following whether food
restriction protects against the negative impact of stress on synaptic plasticity during aging
by evaluating combined effects of stress and food restriction. Another part of the project is
focused on plasticity-related changes in another experimental paradigm, i.e. in response to
general anesthetics in order to elucidate the mechanism(s) by which they cause the
activation of apoptotic and/or survival pathways in the developing rat brain. Gene
expression is followed at the RNA and protein levels by Real Time RT-PCR and Western
blotting combined with immunocytochemistry, respectively.
• Project Website:
Contact Person
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Name:
Email:
Function:
Phone:
Fax:
Website:
Selma Kanazir
[email protected]
Project leader
+381 11 2078 344
+381 11 2761 433
www.ibiss.bg.ac.yu
Research Team Objectives and Resources (list all relevant, but special emphasize
should be made to those related/complementary to NEUROIMAGE)
The objective of our team relevant to NEUROIMAGE is to develop and optimize the
methods to use confocal microscopy to map the proteins involved in neurodegeneration
that colocalize with synaptic plasticity related proteins in various experimental contexts
where plasticity is greatly compromised. For that purpose, resources of CLM, reinforced
through NEUROIMAGE project, will be fully exploited.
• Main Fields: brain plasticity
• Partners/Interests:
• Main resources - instrumentation: Ministry for Science, Republic of Serbia
Institution/University
• Name of Institution/University:
INSTITUTE FOR BIOLOGICAL RESEARCH “S.STANKOVIC”
UNIVERSITY OF BELGRADE
• Number of Employees: ca.250
• Number of Researchers: ca.200
• Profit / Non-profit: non-profit
• Country: SERBIA
• Postal Address:
Molecular Neurobiology Lab.
Neurobiology Dept.
Institute for Biological Research
University of Belgrade
Bul. D. Stefana 142
11060 Belgrade
SERBIA
________________________
FP6 PROJECT “NEUROIMAGE”
SEE Regional Datbase
8. INEP
Research Team
• Research Team Name: Cell Biology of Embryo Implantation
• Individual Researcher:
• Research Team Leader Name: Ljiljana Vićovac
Research Team Members
1. Nikola Kolundžić, B.S.
2. Milica Jovanović, MS (equivalent), PhD student
3. Žanka Bojić, PhD student
4. Ivana Stefanoska, PhD student
5.
Research Team Projects
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Project Name: CELLULAR INTERACTIONS AND MOLECULAR
MECHANISMS IN DIFFERENTIATION OF CELLS AT THE FETOMATERNAL INTERFACE
• Project Leader: Ljiljana Vićovac Panić
• Project Funding Agency: Ministry of Science, Serbia
• Project Budget: NA
• Project Start Date: 01.01.2006.
• Project End Date: 31.12.2010.
• Project Partners:
• Project Summary:
Many aspects of the process of embryo implantation are still insufficiently
understood. Physiological invasion of uterine tissues by human placental trophoblast
is crucial for embryonic development but molecular processes involved remain
largely obscure. Both shallow invasion, which is linked to the pathogenesis of the
maternal hypertensive disorder pre-eclampsia and to fetal growth retardation, as well
as the excessive proliferation and invasion cause serious and life threatening
conditions. Recently, much progress has been made regarding the trophoblast
intrinsic regulatory factors and multitude of cytokines present locally at the time of
implantation. There is, however, still need for further elucidation of the role of some
uterine and other locally present factors on invasive trophoblast differentiation.
It is proposed here to utilize different trophoblast cell models to study the effects of
the selected cytokines, chemokines, hormones or autoantibodies, on trophoblast
differentiation, apoptosis, cell migration and invasiveness. Regulatory factors and
signaling pathways will be studied in vitro at the protein and mRNA level, based on
cellular markers, and functional tests. The project will provide insights into the role of
some decidual products on the differentiation of invasive trophoblast and their
relevance for normal implantation and pregnancy.
• Project Website: www.inep.co.yu
Contact Person
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Name: Ljiljana Vićovac Panić
Email: [email protected]
Function: team leader
Phone: +381-11-619-255
Fax: +381-11-618-724
Website: www.inep.co.yu
Research Team Objectives and Resources
• Main Fields: Biology of Reproduction; Cell Biology of Embryo Implantation; ProteinProtein Interaction
• Partners/Interests: Cell invasiveness,
• Main resources - instrumentation: Cell culture, protein biochemistry, protein
characterization, cell based functional tests
Institution/University
• Name of Institution/University: Institute for Application of Nuclear Energy INEP,
University of Belgrade
• Number of Employees: 85
• Number of Researchers: 40
• Country: Serbia
• Postal Address: INEP, Banatska 31b, 11080 Belgrade-Zemun, Serbia
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