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Activity 15 PS-2820 Muscle Fatigue Physiology: Muscle fatigue Qty 1 1 1 1 DataStudio GLX setup file: muscle.glx Equipment and Materials PASPORT Xplorer GLX PASPORT Force Sensor Timer (stopwatch or equivalent) Rubber ball, tennis ball, or equivalent (approximately 7 cm diameter) Part Number PS-2002 PS-2104 SE-8702B Purpose The purpose of the activity is to compare muscle fatigue in hand muscles due to isotonic and isometric exercise. Background Muscles convert chemical energy into a mechanical force that pulls the muscle shorter and results in movement. All muscles work in pairs by pulling and relaxing; they cannot push. Skeletal muscles are controlled voluntarily. They are the most abundant tissue type in humans. Microscopic examination of this muscle type reveals alternating bands of light and dark stripes, which is why it is sometimes called striated muscle. These muscles are usually attached to bones by tendons and contract when they receive nerve impulses from the brain, allowing bones or cartilage to move. Muscles can also connect to skin, which can be demonstrated by executing facial expressions. Two types of contractions are isotonic and isometric. Most muscle movement involves a combination of both types of contractions. Isotonic (same tension) contractions are produced when heavy objects are lifted. Most body movements use this type of contraction because the muscle must shorten and maintain a constant tension. Isometric (same length) contractions develop when a muscle is not allowed to shorten and the muscle tension increases. Pushing against a fixed object and supporting body weight are two examples of isometric contractions. Muscle fatigue is the consequence of unrelenting work performed by muscles. Under this condition, the expected force that is necessary to maintain the activity diminishes and muscle performance is compromised. Pre-lab Questions Exercise your hand muscles two different ways for equal amounts of time, and measure the grip force of the hand after each type of muscular contraction. Compare your ‘dominant’ hand to the other hand. 1. How will your grip force after one minute of isotonic (same tension) contraction compare to your grip force after one minute of isometric (same length) contraction? 2. How will the grip force of your ‘dominant’ hand after exercise compare to the grip strength of your other hand after exercise? Biology with Xplorer GLX © 2005 PASCO p. 97 Activity 15 Muscle Fatigue PS-2820 Safety Precautions • Stop data collection if the activity of squeezing becomes painful. • Follow all directions for using the equipment. Procedure GLX Setup 1. Connect a PASPORT Force Sensor to the top of the GLX. The Graph Screen will automatically open with Force (N) versus Time (s). 2. Open the GLX setup file labeled muscle.glx (see the appendix at the end of this activity). The setup file is set so the Force Sensor records data at 10 measurements per second. Figure 1: Connect the sensor Sensor Calibration (Optional) • See the appendix at the end of this activity. Equipment Setup 1. For the isotonic exercise (same tension), use a tennis ball (or a rubber ball) that you can squeeze over and over again between the thumb and the middle two fingers of your hand. 2. For the isometric exercise (same length), squeeze the sides of the PASPORT Force Sensor between the thumb and the middle two fingers of your hand. 3. Screw the rubber bumper into the front of the Force Sensor. 4. Practice using the timer. Record Data • Data recording is easier if one person records data and keeps track of the time while another person exercises and is measured. There are three parts to data recording for each hand. • Each trial of data recording and exercising will last 60 seconds (one minute). • When you squeeze the Force Sensor, try to maintain a force of 40 N (newtons). Part A B C Grip Force Measurement Before exercise, ‘dominant’ hand After isotonic exercise, ‘dominant’ hand After isometric exercise, ‘dominant’ hand Grip Force Measurement Before exercise, ‘other’ hand After isotonic exercise, ‘other’ hand After isometric exercise, ‘other’ hand Part A: Grip Force Measurement Before Exercise 1. Put the middle two fingers of your ‘dominant’ hand through the finger holes on the back end of the PASPORT Force Sensor. 2. Press the ZERO button on the Force Sensor and then place your thumb on the rubber bumper. Figure 2: Squeeze Force Sensor Biology with Xplorer GLX © 2005 PASCO p. 98 Activity 15 Muscle Fatigue PS-2820 3. Press the Start key on the GLX. At the same time, start the timer for 60 seconds and begin squeezing the Force Sensor. • Remember; try to maintain a force of 40 N for the entire time of 60 seconds. DO NOT squeeze with your index finger or little finger – only with your thumb on the rubber bumper and the two middle fingers that are in the finger holes of the sensor. 4. Stop recording data and stop squeezing after 60 seconds. 5. Switch to your other hand and repeat the procedure. Part B: Grip Force Measurement After Isotonic Exercise 6. Grip a tennis ball between the middle two fingers and thumb of your ‘dominant’ hand. 7. Start the timer for 60 seconds and begin squeezing the ball as rapidly as possible. • Don’t use your index finger or little finger to ‘help’ – just use your middle two fingers and thumb. Give your thumb a real ‘workout’. 8. At the end of 60 seconds, quickly put the Force Sensor on your hand as before. Press the ZERO button on the sensor and then put your thumb on the rubber bumper. 9. Press the Start key on the GLX. At the same time, start the timer for 60 seconds and begin squeezing the Force Sensor. • Try to maintain a force of 40 N for the entire time of 60 seconds. DO NOT squeeze with your index finger or little finger – only with your thumb and two middle fingers. 10. Stop recording data and stop squeezing after 60 seconds. 11. Switch to your other hand and repeat the same procedure. Figure 3: Isotonic exercise Part C: Grip Force Measurement After Isometric Exercise 12. Grip the sides of the Force Sensor between the middle two fingers and thumb of your ‘dominant’ hand. 13. Start the timer for 60 seconds and begin squeezing the sensor between your middle two fingers and thumb as hard as possible. 14. At the end of 60 seconds, quickly put the Force Sensor on your hand as before. Press the ZERO button on the sensor and then put your thumb on the rubber bumper. 15. Press the Start key on the GLX. At the same time, start the timer for 60 seconds and begin squeezing the Force Sensor. • Try to maintain a force of 40 N for the entire time of 60 seconds. 16. Stop recording data and stop squeezing after 60 seconds. 17. Switch to your other hand and repeat the procedure. Biology with Xplorer GLX © 2005 PASCO Figure 4: Isometric exercise p. 99 Activity 15 Muscle Fatigue PS-2820 Analysis 1. Use the built-in analysis tools in the Graph Screen to determine the beginning grip force, the final grip force, and the average grip force for each run of data. 2. Record the beginning, final, and average values of grip force the Data Table. 3. Determine if there are differences in the changes in grip force (if any) between males and females. Record your results in the Lab Report section. ---------------- Appendix To open a specific GLX file, go to the home screen (press ). In the home screen, select ‘Data Files’and press ‘Activate’ ( ). Use the cursor keys to navigate to the file you want. Press F1 ( ) to open the file. Optional: To calibrate the PASPORT Force Sensor, see the instructions provided by the instructor. Biology with Xplorer GLX © 2005 PASCO p. 100 Activity 15 Muscle Fatigue PS-2820 Lab Report - Activity 15: Muscle Fatigue Name ________________________________ Date ___________ Pre-lab Questions Exercise your hand muscles two different ways for equal amounts of time, and measure the grip force of the hand after each type of muscular contraction. Compare your ‘dominant’ hand to the other hand. 1. How will your grip force after one minute of isotonic (same tension) contraction compare to your grip force after one minute of isometric (same length) contraction? 2. How will the grip force of your ‘dominant’ hand after exercise compare to the grip strength of your other hand after exercise? Data Make a sketch of one run of Force versus Time, including labels for the y- and x-axes. Data Table Part Grip Force Measurement – Dominant Hand A Before exercise, ‘dominant’ hand B After isotonic exercise, ‘dominant’ hand C After isometric exercise, ‘dominant’ hand Part Grip Force Measurement – Other Hand A Before exercise, ‘other’ hand B After isotonic exercise, ‘other’ hand C After isometric exercise, ‘other’ hand Biology with Xplorer GLX Beginning Final Average Beginning 43.4 N 45.6 N 52.2 N Final 37.5 N 28.3 N 23.6 N Average 38.9 N 34.1 N 35.4 N © 2005 PASCO p. 101 Activity 15 Muscle Fatigue PS-2820 Questions 1. What happened to your grip force after you did the exercise? 2. How did your grip force change when you did the isotonic exercise compared to when you did the isometric exercise? 3. Were the results different for the dominant and the non-dominant hand after 60 seconds? Explain. 4. What can you determine about the rate of muscle fatigue between males and females in your class data? Explain. 5. Describe how your thumb and hand felt at the end of each activity. 6. Name an activity where your body loses the ability to deliver adequate oxygen to the muscles. 7. Another word for “without oxygen” is: anaerobic. During this cycle of oxygen debt, what product accumulates in the muscle tissues? 8. Do you think that frequently used muscles would tire faster or slower than muscles that are not used as regularly? Explain. Biology with Xplorer GLX © 2005 PASCO p. 102