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Editorials See related article, pages 32–39 Electrophysiological Profiling of Cardiomyocytes in Embryonic Bodies Derived From Human Embryonic Stem Cells Therapeutic Implications Rachel D. Vanderlaan, Gavin Y. Oudit, Peter H. Backx H Downloaded from http://circres.ahajournals.org/ by guest on June 18, 2017 observed variability may reflect differences in plating density, culture medium composition, or period of gestation.13 Another factor that may influence cardiomyocyte differentiation is the inductive interactions between anterior visceral endoderm-like cells and cardiac progenitor cells.14 He and colleagues used an array of tools to phenotype hES cell– derived cardiomyocytes. Immunostaining and ultrastructural analysis established that beating EBs displayed a sarcomeric pattern and Z-lines as reported previously by others.8,13,14 Previous reports have also demonstrated that beating EBs express thin- (cardiac troponin I and T) and thickfilament (myosin heavy and light chains) proteins, creatine kinase-MB, cardiac transcription factors such as GATA-4, Nkx2.5, and MEF-2, and the cardiac-specific marker ANF.8,13,14 Collectively, these findings suggest that EBs contain cardiomyocytes. Consistent with this suggestion, in situ action potentials (APs) within a beating outgrowth recorded using microelectrode impalements (intracellular recordings) revealed typical cardiac-like depolarization patterns with AP heterogeneity resembling AP profiles observed in nodal, atrial, and ventricular cardiomyocytes. These observations build on recent findings showing similar electrical heterogeneity in hES cells cultured with visceral endodermlike cells.14 Electrical heterogeneity was also observed as two distinct types of spontaneous electrical activity (ie, continuous and episodic) in EBs,11 which may depend on intercellular connexin-containing gap junction expression.12 The authors speculate that the episodic pattern could be due to conduction block related to tissue geometry, impaired cellto-cell coupling, or reduced cellular excitability, although the role of altered intracellular Ca2⫹ cycling due to an immature Ca2⫹ regulatory system cannot be ruled out. The phenotyping and segregation of hES cell– derived cardiomyocytes into different subpopulations may allow highly specific cell therapies to be developed thereby minimizing possible side effects. For example, nodal cells may be used for pacemaker support in sick sinus syndrome and atrioventricular block, whereas the use of “pure” ventricular cells might allow selective therapy of myocardial diseases without the creation of a nidus of proarrhythmogenic pacemaker/nodal cells. How undifferentiated hES cells transform into different types of cardiac myocytes will clearly be an important area of future research. Indeed, the ability of the vascular cytokine, endothelin, to transform avian embryonic heart muscle cells into impulse-conducting Purkinje fibers provides evidence that humoral factors can induce electrically distinct cell populations in the heart.16 uman embryonic stem (hES) cells are derived from the inner mass cells of developing blastocytes and have the ability to generate cells from three embryonic germ layers. Since their initial culturing in 1981, ES cells have revolutionized the mouse genetics field1,2 by allowing the creation of mouse models of disease as well as the molecular study of the differentiation of pluripotent cells into various somatic cell types including cardiomyocytes and vascular smooth cells.3–5 Some key features of ES cells that have made them particularly useful in research include their ability for self-renewal, diploid karyotype stability, and continuous telomerase activity.6 –9 Since human fetuses are not generally available for scientific study, hES cells represent an in vitro model for embryonic differentiation, with the ability to understand more about cell lineage commitment and the process of differentiation. Importantly, they also represent a potential source of cells for therapeutic uses such as the regeneration of functional myocardium and conducting tissue. As a consequence of these capabilities and their derivation from human embryos, hES cells have been a subject of numerous ethical and moral debates.10 In this issue of Circulation Research, He and coworkers characterize the electrophysiological and contractile properties of cardiomyocytes derived from hES cells.11 This work, together with previous studies by Gepstein’s group8,12 and other recent reports,13,14 expands our understanding of cardiac differentiation of hES cells, building a foundation for future research and for new therapeutic strategies using hES cells. In agreement with other hES studies,8 He et al found that about 20% of the embryoid bodies (EBs) (three-dimensional cell aggregates of hES cells in culture) remained as spontaneous beating “cardiac-like” cell clusters after 45 days in culture. The number of cardiomyocytes within these EB outgrowths varied widely (between 2% to 70%). Others have previously reported that up to 70% of human EBs can be induced to beat spontaneously after 16 days in culture13 whereas approximately 90% of mouse EBs beat after differentiation.15 This The opinions expressed in this editorial are not necessarily those of the editors or of the American Heart Association. From the Departments of Medicine and Physiology, Heart and Stroke/ Richard Lewar Centre of Excellence, University of Toronto, Ontario, Canada. Correspondence to Dr Peter H. Backx, DVM, PhD, Heart and Stroke/Richard Lewar Centre of Excellence, 150 College St, Room 68, Fitzgerald Building, Toronto, Ontario, M5S 3E2. E-mail [email protected] (Circ Res. 2003;93:1-3.) © 2003 American Heart Association, Inc. Circulation Research is available at http://www.circresaha.org DOI: 10.1161/01.RES.0000082767.38055.03 1 2 Circulation Research July 11, 2003 Downloaded from http://circres.ahajournals.org/ by guest on June 18, 2017 Functional aspects of hES cell– derived cardiomyocytes were determined by He and colleagues using two different approaches. First, by increasing the EB outgrowth pacing rates, these authors observed AP shortening adaptation in ventricular-like cardiomyocytes; this physiological response leads to systolic shortening at high heart rates thereby maintaining diastolic time for ventricular filling as shown in ventricular myocardium of human embryos.17 Second, the impact of the specific IKr blocker, E-4031,18 on AP profile and triggered arrhythmias (early [EAD] and delayed [DAD] afterdepolarizations) was examined. Human cardiac APs represent complex interactions between depolarizing (such as Na⫹ and Ca2⫹ currents) and repolarizing currents (such as transient outward [Ito] and delayed rectifier [IKr and IKs] K⫹ currents).18,19 The application of E-4031 led to prolongation of atrial- and ventricular-like APs thereby providing pharmacological evidence that IKr contributes to repolarization in these myocytes. These data complement recent studies in hES cells showing expression of KvLQT1 (pore-forming subunit of IKs) and Kv4.3 (pore-forming subunit of Ito).14 Future studies will undoubtedly unravel the temporal and spatial expression patterns of pore-forming and accessory subunits contributing to cardiac ion channels in hES cell– derived cardiomyocytes, which are known to be developmentally regulated.19,20 In addition, although EBs are only 4 to 10 layers thick, it will be of great interest to determine whether regional “transmural” variations in AP profile occur within EBs, thus providing important new approaches for studying the regulatory factors governing the three-dimensional organization of AP profile and cardiac electrophysiology. The application of E-4031 and the associated AP prolongation was also associated with EADs and DADs, suggesting that these myocytes have the capacity for arrhythmogenesis. Although this may be related to spontaneous Ca2⫹ release,19 it could also be at least partially related to myocyte injury after microelectrode impalement. In either case, relative high expression/activity of the Na⫹-Ca2⫹ exchanger observed in fetal/neonatal myocytes is likely to play an important role in the formation of these arrhythmias.21,22 Activation of G protein– coupled receptors, including -adrenergic (1- and 2-AR), ␣-adrenergic, and muscarinic (M2) receptors, is known to strongly influence contractile properties and beating rates of cardiomyocytes derived from hES.13,14 Consistent with this, He and coworkers showed that the acute application of the -adrenergic agonist, isoproterenol, leads to a marked positive inotropic effect in hES cell– derived EB outgrowths.11 These effects could be related to activity of protein kinase A on L-type Ca2⫹ channel (␣1c) and phospholamban, both of which are expressed in these preparations14 and are likely to elevate peak systolic intracellular Ca2⫹ concentration recorded previously in these preparations.8 In addition, the L-type Ca2⫹ channel blockers, diltiazem and verapamil, have profound negative chronotropic effects on hES cell– derived cardiomyocytes13,14 as expected from the observation that pacemaker activity is critically dependent on L-type Ca2⫹ channel current.23 Overall, this study by He et al,11 together with several other recent reports, provides a solid phenotypic analysis of the electrophysiological and contractile features of EB-derived cardiomyocytes and underscores the complex machinery that exists within these cells. The observation that fetal myocyte transplantation can lead to stable intracardiac grafts and improvement of cardiac function24 has stimulated interest in the use of ES cells, with their self-renewal and pluripotent characteristics, as a potential therapeutic tool for cardiovascular diseases. Because heart disease and failure are often characterized by a loss of functioning cardiomyocytes, which are terminally differentiated and show a very limited capacity for regeneration,25 transplantation of hES cells or cardiac grafts may be a means of reversing the functional changes observed in cardiac patients. In particular, the study by He et al suggests that it might be possible to introduce preselected nodal, atrial, or ventricular myocytes to appropriately affected regions, thus tailoring therapy for heart disease patients. While the idea has conjured much interest, many questions regarding the feasibility of cell-based therapy still exist. Questions concerning proarrhythmogenic integration, functional coupling with host tissues in context of the extracellular matrix, the role of epigenetic factors (eg, hemodynamics), and rejection and vascularization of the graft (eg, concomitant use of endothelial precursor cells) will all need to be addressed.9,24,26 Undoubtedly, a complete understanding of the electrophysiology and excitation-contraction coupling in hES cell– derived cardiomyocytes will provide insights into human embryonic cardiomyocyte development, signaling pathways, and transcriptional events while fostering the potential therapeutic applications of hES cells. References 1. Evans MJ, Kaufman MH. Establishment in culture of pluripotential cells from mouse embryos. Nature. 1981;292:154 –156. 2. Martin GR. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci U S A. 1981;78:7634 –7638. 3. Doetschman TC, Eistetter H, Katz M, Schmidt W, Kemler R. The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J Embryol Exp Morphol. 1985;87:27– 45. 4. Maltsev VA, Wobus AM, Rohwedel J, Bader M, Hescheler J. Cardiomyocytes differentiated in vitro from embryonic stem cells developmentally express cardiac-specific genes and ionic currents. Circ Res. 1994;75:233–244. 5. Drab M, Haller H, Bychkov R, Erdmann B, Lindschau C, Haase H, Morano I, Luft FC, Wobus AM. From totipotent embryonic stem cells to spontaneously contracting smooth muscle cells: a retinoic acid and db-cAMP in vitro differentiation model. FASEB J. 1997;11:905–915. 6. Carpenter MK, Rosler E, Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells. 2003;5:79 – 88. 7. Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM. Embryonic stem cell lines derived from human blastocysts. Science. 1998;282:1145–1147. 8. Kehat I, Kenyagin-Karsenti D, Snir M, Segev H, Amit M, Gepstein A, Livne E, Binah O, Itskovitz-Eldor J, Gepstein L. Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes. J Clin Invest. 2001;108:407– 414. 9. Gepstein L. Derivation and potential applications of human embryonic stem cells. Circ Res. 2002;91:866 – 876. 10. Robertson JA. Human embryonic stem cell research: ethical and legal issues. Nat Rev Genet. 2001;2:74 –78. 11. He JQ, Ma Y, Lee Y, Thomson JA, Kamp TJ. Human embryonic stem cells develop into multiple types of cardiac myocytes: action potential characterization. Circ Res. 2003;93:32–39. 12. Kehat I, Gepstein A, Spira A, Itskovitz-Eldor J, Gepstein L. Highresolution electrophysiological assessment of human embryonic stem cell-derived cardiomyocytes: a novel in vitro model for the study of conduction. Circ Res. 2002;91:659 – 61. Vanderlaan et al Downloaded from http://circres.ahajournals.org/ by guest on June 18, 2017 13. Xu C, Police S, Rao N, Carpenter MK. Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells. Circ Res. 2002;91:501– 8. 14. Mummery C, Ward-Van Oostwaard D, Doevendans P, Spijker R, Van Den Brink S, Hassink R, Van Der Heyden M, Opthof T, Pera M, De La Riviere AB, Passier R, Tertoolen L. Differentiation of human embryonic stem cells to cardiomyocytes: role of coculture with visceral endoderm-like cells. Circulation. 2003;107:2733–2740. 15. Fijnvandraat AC, van Ginneken AC, de Boer PA, Ruijter JM, Christoffels VM, Moorman AF, Lekanne Deprez RH. Cardiomyocytes derived from embryonic stem cells resemble cardiomyocytes of the embryonic heart tube. Cardiovasc Res. 2003;58:399 – 409. 16. Gourdie RG, Wei Y, Kim D, Klatt SC, Mikawa T. Endothelin-induced conversion of embryonic heart muscle cells into impulse-conducting Purkinje fibers. Proc Natl Acad Sci U S A. 1998;95:6815– 6818. 17. Jezek K, Pucelik P, Sauer J, Bartak F. Basic electrophysiological parameters and frequency sensitivity of the ventricular myocardium of human embryos. Physiol Bohemoslov. 1982;31:11–19. 18. Tristani-Firouzi M, Sanguinetti MC. Structural determinants and biophysical properties of HERG and KCNQ1 channel gating. J Mol Cell Cardiol. 2003;35:27–35. 19. Oudit GY, Kassiri Z, Sah R, Ramirez RJ, Zobel C, Backx PH. The molecular physiology of the cardiac transient outward potassium current (Ito) in normal and diseased myocardium. J Mol Cell Cardiol. 2001;33: 851– 872. Cardiac Development in Human Embryonic Bodies 3 20. Franco D, Demolombe S, Kupershmidt S, Dumaine R, Dominguez JN, Roden D, Antzelevitch C, Escande D, Moorman AF. Divergent expression of delayed rectifier K⫹ channel subunits during mouse heart development. Cardiovasc Res. 2001;52:65–75. 21. Haddock PS, Coetzee WA, Artman M. Na⫹/Ca2⫹ exchange current and contractions measured under Cl⫺-free conditions in developing rabbit hearts. Am J Physiol. 1997;273:H837–H846. 22. Balaguru D, Haddock PS, Puglisi JL, Bers DM, Coetzee WA, Artman M. Role of the sarcoplasmic reticulum in contraction and relaxation of immature rabbit ventricular myocytes. J Mol Cell Cardiol. 1997;29: 2747–2757. 23. Lipsius SL, Huser J, Blatter LA. Intracellular Ca2⫹ release sparks atrial pacemaker activity. News Physiol Sci. 2001;16:101–106. 24. Dowell JD, Rubart M, Pasumarthi KB, Soonpaa MH, Field LJ. Myocyte and myogenic stem cell transplantation in the heart. Cardiovasc Res. 2003;58:336 –350. 25. Nadal-Ginard B, Kajstura J, Leri A, Anversa P. Myocyte death, growth, and regeneration in cardiac hypertrophy and failure. Circ Res. 2003;92: 139 –150. 26. Nir SG, David R, Zaruba M, Franz WM, Itskovitz-Eldor J. Human embryonic stem cells for cardiovascular repair. Cardiovasc Res. 2003; 58:313–323. KEY WORDS: embryonic stem cells 䡲 embryonic bodies 䡲 cardiomyocytes 䡲 action potentials 䡲 electrophysiology Electrophysiological Profiling of Cardiomyocytes in Embryonic Bodies Derived From Human Embryonic Stem Cells: Therapeutic Implications Rachel D. Vanderlaan, Gavin Y. Oudit and Peter H. Backx Downloaded from http://circres.ahajournals.org/ by guest on June 18, 2017 Circ Res. 2003;93:1-3 doi: 10.1161/01.RES.0000082767.38055.03 Circulation Research is published by the American Heart Association, 7272 Greenville Avenue, Dallas, TX 75231 Copyright © 2003 American Heart Association, Inc. All rights reserved. Print ISSN: 0009-7330. 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