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SAS-1+ O P E R AT O R S M A N U A L English SAS-1 Plus Operators Manual Contents 1 2 3 4 5 6 7 The SAS-1 Plus 1.1 Introduction 1.2 Informational symbols 1.3 Precautions and Limitations 1.4 Safety Considerations 3 3 3 4 Installation 2.1 Instructions for installation 5 Controls 3.1 3.2 3.3 6 6 6 Mains Supply Front Panel Application Drawer Operating Instructions 4.1 Power up 4.2 Program selection 4.3 Prepare for electrophoresis 4.4 Start an application 4.5 Create or Edit a program 4.6 Edit or Change a program name 4.7 View process parameters during a program run 4.8 Abort a program run 7 7 8 9 11 12 13 14 Maintenance 5.1 Heat sink 5.2 Carbon electrodes 5.3 Antisera template 5.4 Sample tray 5.5 Cleaning spills 5.6 Replace fuse(s) 15 15 15 15 15 15 Troubleshooting 6.1 Problem, Cause and Solution 16 Performance Specification 7.1 Specifications and parameters 17 Warranty Report 18 2 English SAS-1 Plus Operators Manual 1 The SAS-1 Plus 1.1 Introduction The SAS-1 Plus is a laboratory instrument, designed to perform an electrophoresis process on Helena BioSciences Europe agarose products. The device incorporates automated sample application, electrophoresis and incubation. This manual provides all necessary information for the setup and operation of the SAS-1 Plus including important information regarding safety and maintenance of the unit. To ensure safe and reliable operation, always operate the SAS-1 Plus in accordance with the operator manual. READ SECTIONS 1.3 (PRECAUTIONS AND LIMITATIONS) AND 1.4 (SAFETY CONSIDERATIONS) OF THIS MANUAL BEFORE ATTEMPTING INSTALLATION AND OPERATION. Helena BioSciences Europe is not responsible for injuries or damages caused by improper use. 1.2 Informational Symbols Warning The operators manual should be consulted to avoid possible personal injury or instrument damage. Caution, high voltage! Indicates an area where a potential electrical shock hazard may exist. 1.3 Precautions and Limitations To isolate the equipment, remove the plug from the socket. The mains switch is considered to be the primary disconnect device and should be easily accessible by the operator. > > > > > The entire operators manual should be read and understood before attempting instrument operation. This instrument is designed for use with Helena BioSciences Europe products only. Always check the parameters set in the SAS-1 Plus program before starting. The parameters may be different than those prescribed in the Instructions For Use. The parameters can be accessed via the edit-function. Ensure the fan at the rear of the unit is not covered and has adequate air circulation. Ensure that the electrodes and the sample tray are removed from the instrument prior to any transportation. 3 English SAS-1 Plus Operators Manual > This piece of equipment must not be used in areas of high electromagnetic phenomenon as temporary loss of function may occur. Contact Helena Biosciences Europe for advice. 1.4 Safety Considerations The SAS-1 Plus is fully overload protected. However, the following guidelines should be observed to ensure safe operation of the unit. > > > > > The SAS-1 Plus must only be operated when connected to an earthed supply of the correct voltage and frequency as indicated on the serial number label. For emergency shutdown, use either the ON/OFF (switch on the rear panel of the device), or switch off at the mains supply. Wait at least 5 seconds after stopping a run before handling output leads or connected apparatus. Always make sure your work area is clean and dry before operating the SAS-1 Plus. If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired. To avoid damage to the instrument: > > > > > > > > For proper ventilation, leave at least 10 cm of space behind the instrument and on either side. Do not operate the SAS-1 Plus in high humidity environments (>90%), or where condensation may occur. To avoid condensation, the unit should be left on between use during operation in a cold room or in high humidity environments. Do not lubricate any parts of the instrument. Never place objects on top of the instrument. Keep flammable liquids and flammable vapors away from the instrument at all times. The Drawer door can be opened easily during electrophoresis. This is not recommended as the instrument immediately aborts the program and displays the error-message “Tray moved”. The voltage supply will be shut off immediately but it is still possible to get an electrical shock straight after opening the drawer if the electrode posts are touched. Wait for at least one minute to ensure that there is no risk of electrical shock. The drawer and lift arms present no entrapment hazard, however it is recommended that no objects are placed in the vicinity of the drawer whilst in operation. 4 English SAS-1 Plus Operators Manual 2 Installation Please verify upon receiving the SAS-1 Plus that no damage has occurred during transit and confirm that the following components and accessories are included: > > > > > > Item No. Units Ref. No. SAS-1 Plus or Cat. No. 1532 (115V) Operator manual, including Warranty report Spare fuses 1 unit Cat. No. 1531 (230V) 1 unit 2 units Power cord 1 unit Carbon electrodes Sample Tray Gel Block Remover Antisera template Gel cover 2 units 1 unit 1 unit 1 unit 1 unit (art. no. 0100.076) (art. no. 6000.315) or (art. no. 6000.630) (art. no. 7100.001) or (art. no. 7100.002) or (art. no. 7100.006) (art. no. 0030.366) (art. no. 0030.602) (art. no. 8100.025) (art. no. 0030.650) (art. no. 0030.405) Place the SAS-1 Plus unit on a level bench. Keep the area around the instrument clear to ensure correct ventilation. Select an environment free from excessive humidity, dust and large temperature fluctuations. Allow the instrument to acclimatise to room temperature for a period of at least 2 hours before operating. The power cord attachment module is located at the rear of the unit. Position the unit so this is easily accessible. Remove the transport pin and plug from the drawer, located on the bottom side of the instrument. Store in a safe place for possible re-use should the device require further transportation. Connect the unit to a suitable earthed mains outlet of the correct voltage and frequency. This unit MUST be earthed. Turn on the main power switch located on the left side of the instrument. 5 English SAS-1 Plus Operators Manual 3 Controls 3.1 Mains Supply ON/OFF Switch This is a rocker-type switch, which controls the mains input to the unit and is situated on the rear of the instrument. 3.2 Front Panel Display Unit LCD operator interface providing information of program parameters and machine status. LED Indicates that the current is flowing between the electrodes. Enter Press to confirm selection of a program. Press to enter the editable parameters in a program. Press to confirm values in manual programming. + Press to begin electrophoresis. Press to scroll up through program menu. Press to increase the number of any editable setting. - Press to cancel electrophoresis before run is started. Press to scroll down through program menu. Press to decrease the number of any editable setting. Stop Press to stop operation and return to standby mode. Press to end the edit page function. Confirmation of an operator adjustable program name. 3.3 Application Drawer Location mount The location mount is for the positioning of the sample tray. Electrode Posts The electrode posts carry the voltage from the power supply to the electrodes. The posts are also magnetic to hold the electrodes in place. The positive electrode posts are located at the front of the drawer, the negative at the rear. Heat sink The heat sink carries heat away from the gel and is also used to position the gel. The heat sink is adapted with a peltier device that controls the temperature between 15°C and 45°C. 6 English SAS-1 Plus Operators Manual 4 Operating Instructions 4.1 Power up Connect the unit to an appropriate mains outlet and switch on by using the power switch located on the rear of the instrument. The display will prompt: Helena BioSciences SAS-1 Plus This shows that the machine is ready to operate. 4.2 Program selection Press ANY KEY to continue. The display will prompt: Select required test Use +/- then ENTER Use the ‘+’ and the ‘-’ keys to scroll between programs. Press ENTER to select desired program. The display will prompt and alternate between: SAS program Press + to Start and SAS program Press - to Cancel and 7 English SAS-1 Plus Operators Manual SAS program Press ENTER to edit > > > Press ‘-’ to cancel and return to the program selection prompt. Press ENTER to edit or view the parameters of the selected program. Press ‘+’ to start the application. NOTE: Always check the parameters in the SAS-1 Plus program before starting. The parameters may differ to those prescribed in the Instructions For Use, supplied with the electrophoresis kit. 4.3 Prepare for electrophoresis The SAS-1 Plus must be prepared for the required application before the program commences. Refer to the Instructions For Use supplied with each kit for full operator instructions. NOTE: Always put the Sample in the Sample row as displayed in figure 1 below. The Dilution cups are for manual use only, the SAS-1plus is not able to reach the Dilution rows Fig.1 8 English SAS-1 Plus Operators Manual 4.4 Start an application Before starting the electrophoresis, verify the following. The sample gels are orientated correctly and the sample cups are installed correctly and fully seated in the sample tray. The applicator blades are properly installed and seated in the slots. After selecting a program, as described in section 4.2, press ‘+’ to start. The drawer opens and the display will prompt: SAS program Press key to close NOTE: Refer to the Instructions For Use for specific gel preparation. Place the sample tray into the instrument, apply REP Prep, place the gel into position and blot, then place the electrodes into the correct position. Place the gel cover over the gel and electrodes, press for 5 seconds to ensure good electrode contact. Applicator Blades Gel Cover Electrodes Gel Sample Cup Holder 9 English SAS-1 Plus Operators Manual Press any key to continue. The instrument will then perform the following steps: 1. Pick up sample from the sample tray. 2. Load sample on the gel. 3. Apply Voltage for a timed period. When no incubation is programmed the instrument will sound and display: Electr. completed Press key to open Electrophoresis is complete and the gel is ready for further processing. When incubation is programmed, the SAS-1 Plus will sound and alternate between the displays shown below: Incubation step 1 Press + to open and Incubation step 1 Press - to continue Follow the Instructions For Use for reagent preparation. When ready Press ANY KEY to commence incubation. It is possible to program up to 9 incubation steps with different times and temperatures. Between incubation steps, the drawer opens to allow for any further action by the operator. After incubation is complete, the instrument will sound, and any key may be pressed to open the drawer for gel removal. Process completed Press key to open 10 English SAS-1 Plus Operators Manual 4.5 Create or Edit a program At program selection (see section 4.2) the screen will prompt: SAS program Press ENTER to edit select ENTER and the prompt will read: SAS program EDIT-PAGE application nr. 2 The cursor blinks on the first position of the second line. At this cursor position it is possible to use the +/- keys to scroll through the following program parameters: SAS-1 Plus parameter Parameter description Value (min / max) SAS program Edit Program name 10 digits max Application no. Number of Applications 0 - 12 Electr. voltage Electrophoresis voltage 50V - 600V Electr. time Electrophoresis time 0 min - 99 min Electr. Temp. Electrophoresis temperature 15°C - 45°C or OFF Incubation steps* Number of incubation steps 1 - 9 steps & OFF Incubation time 1** Incubation time for step 1 0 min - 99 min Incubation temp.1 Incubation temperature for step 1 15°C - 45°C * if this parameter is set to OFF, no incubation programming or use of the Add Reagent function is possible. ** up to a maximum of 9 steps. Press the Enter key to edit a parameter The cursor will automatically go to the setting, which can be altered with the +/- keys, within the limits that are specified above. When the parameter is set, press enter to leave parameter editing and return to scrolling between the parameters. 11 English SAS-1 Plus Operators Manual 4.6 Edit or Change a program name To edit the program name, use the - key until you reach the following prompt: SAS program EDIT-PAGE Program name When pressing ENTER the cursor will go to the first digit of the name, which can be changed with the +/- keys. When the digit is correct press ENTER and the cursor will go to the next digit. All digits need to be confirmed before the cursor returns to its start position. Use the STOP key to leave the edit-page. The prompt will read and alternate between: SAS program EDIT-PAGE Press + to save and: SAS program EDIT-PAGE Press - to cancel After pressing the + or - key the prompt will return to start position and the operater can choose to start, cancel or edit the program. 12 English SAS-1 Plus Operators Manual 4.7 View process parameters during a program run During a program run it is possible to view the process parameters on the display. The standard display states the time left. Time left = 18m38s Electr. in Progress Using the +/- keys it is possible to scroll through different values and show the following screens. Actual temp = 20C Electr. in Progress Output voltage = 600V Electr. in Progress Output current = 20mA Electr. in Progress The second line of the display will alternate between the actual program step (electrophoresis or incubation) and “press STOP to abort”. 13 English SAS-1 Plus Operators Manual 4.8 Abort a program run To abort a program run at any stage, the STOP key should be pressed, NOTE: it is not possible to pause any stages of the program. Press any key to open. The drawer will open and the gel, electrodes and sample tray can be removed. The display will prompt: Electro. aborted Press key to close This ends the program and the display will prompt: SAS program Press + to start 14 English SAS-1 Plus Operators Manual 5 Maintenance This section describes routine operators maintenance procedures. For instrument calibration or for maintenance not described in this manual, call Helena BioSciences Europe for assistance. 5.1 Heat Sink After each use dampen a lint-free tissue with deionised water and wash the surface of the heat sink. 5.2 Carbon electrodes After each use rinse the carbon electrodes in deionised water and dry with a lint-free tissue. 5.3 Antisera template After each use, clean the template with detergent, deionised water and a soft brush. Rinse thoroughly with water and dry with a lint-free tissue. 5.4 Sample tray If the tray becomes contaminated with blood or blood derivatives, spray with a commercial virucidal and germicidal agent. Rinse thoroughly with water and dry with a lint-free tissue. 5.5 Cleaning Spills Clean spills with a soft cloth or sponge ONLY after switching off the power and unplugging the power cord. Do not use corrosive cleaners. Dry the unit before plugging in the power cord. 5.6 1. 2. 3. 4. 5. 6. Replace fuse Switch off the device and remove the mains cable from the input. Withdraw the fuse carrying tray from the fuse holder and remove the fuses. Replace with fuses of the correct type and rating. Replace fuse holder. Plug power cord back into the instrument and switch on the power. If fuses blow immediately contact Helena BioSciences Europe for assistance. 15 English SAS-1 Plus Operators Manual 6 Troubleshooting NOTE: If problem persists or any other messages are displayed on the screen, contact Helena BioSciences Europe for assistance. Problem Cause Solution No Power to the Instrument Fuse(s) blown Replace fuse(s) Error Message “Tray pos. Failure” Instrument failed to position tray correctly Switch instrument off and on Error Message “Lift pos. failure” Instrument failed to position lift correctly Switch instrument off and on Error Message “Power Failure” Power Supply has failed during a run Results might not be correct Press any key to continue Error Message “Overload!” Current during electrophoresis is too high Check the programmed voltage Error message “Tray Moved” Drawer moved during electrophoresis Press any key to continue Error message “No Load detected” Poor electrode contact Ensure the electrodes are in complete contact with the gel buffer blocks and the electrode posts Error message “Temp. not reached” Peltier unit failed to reach set temperature Programmed temperature is out of specifications (more than 5°C below ambient temperature Poor electrophoresis results Poor electrode contact Ensure the electrodes are incomplete contact with the gel buffer blocks Check program steps Electrophoresis procedure has not been performed correctly Check electrophoresis setting Check electrophoresis temp. Check and/or replace contact sheet Check and/or replace electrodes Contact your local distributor 16 English SAS-1 Plus Operators Manual 7 Performance Specifications Input Power: 230 Vac (Cat. No. 1531) 115 Vac (Cat. No. 1532) Frequency 50/60 Hz, 225 watts Voltage is factory set (see Serial No. Label). Environmental conditions: Operating temperature 15 till 27°C. Relative humidity of max 90% (non condensing). Display: 2 x 20 character LCD + LED for HV-indication. Keypad: 4 keys Operating Modes: Standby, Program selection, Program editing and Program run. Programmable Run parameters: Electrophoresis Voltage: Set-value: Accuracy: Noise and Ripple: Power: 50 - 600 Volts (increments 5 Volt) within ± 2% Better than 5 % of output voltage 50 -300 Volt at max. 100 mA or 30 Watt 300 - 600 Volt at max. 50 mA or 30 Watt Electrophoresis & incubation Timers: Set value: from 0 to 99 minutes: 1 minute increments. Better than +/- 1 % of setting. Accuracy: Temperature control: Set Value: Accuracy: Stability: 15°C till 45°C (at Ambient temperature of 20°C) ± 1°C ± 1°C Program features: Possibility to Add Reagents manually during program. Possibility to program up to 9 incubation periods. Safety features: Safety Interlock breaks electrophoresis circuit when applicator drawer door is open. Process control: Current overload protection. No load detection. Check set temperature reached. Dimensions: Weight: Size: Fuse - Mains: 230 Vac 1 AT 5 x 20mm Spiral 115 Vac 2 AT 5 x 20mm Spiral 2 fuses required (live and neutral fused) approx. 13 kilo 24 cm x 33 cm x 26 cm (WxDxH) 17 English SAS-1 Plus Operators Manual Warranty Please complete and return to Helena BioSciences Europe, by email, fax or post. Instrument Name: Date Received: Catalogue Number: Serial Number: Name: Department: Institution: Address: Post Code: Telephone Number: Ext. (where applicable): Email: Helena warrants each unit to be free from defects in material and workmanship. Helena’s liability under this warranty is limited to servicing or adjusting any unit and to replacing any defective part. This warranty is effective for 1 year commencing from the date of shipment to the original purchaser, and does not cover faults caused by misuse, abnormal conditions, damage incurred in shipment, or consumable items such as lamps. This warranty is made in lieu of all other warranties, expressed or implied, and is limited in any sale to the repair or replacement of the affected component. 18 English SAS-1 Plus Operators Manual Condition of Equipment on Arrival In order to evaluate shipping procedures and conditions of equipment on arrival, please complete this report and return to us. 1. Did the instrument arrive in apparent good condition? Yes [ ] 2. No [ ] Will you be able to operate this instrument without additional information? Yes [ ] No [ ] Comments: Helena BioSciences Europe is committed to providing you with the finest equipment and supplies possible. If you need assistance please call our Sales / Service Department: tel: +44 (0) 191 482 8440 fax: +44 (0) 191 482 8442 email: [email protected] www.helena-biosiences.com 19 English Helena BioSciences Europe Queensway South Team Valley Trading Estate Gateshead Tyne and Wear NE11 0SD tel: +44 (0)191 482 8440 fax: +44 (0)191 482 8442 email: [email protected] HL-2-1512P 2006/06 (2)