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BIOLOGY 103 – LABORATORY EXERCISE 5 Name: Day & Time of Assigned Lab: Seat Number: Flower Structure Introduction: The Angiosperms, or flowering plants, represent the most successful and most diverse Division within the Plant Kingdom. The estimated number of species of flowering plants is in excess of 250,000 named species; about 90% of all plants are angiosperms. It is not surprising that the plants that we are most familiar with, and most dependent on are flowering plants. Flowers are reproductive structures. It is interesting that to show a sentiment of love we send our intended a bunch of plant sexual organs. Flowers evolved about 125 million years ago. The early flowers were probably beetle pollinated as beetles unintentionally transferred pollen from flower to flower as they ate pollen. Following this discovery the number of species of flowering plant, and insects that pollinate flowers, such as butterflies, rapidly increased. Fertilization of female ovules by male sperm cells carried inside pollen, leads to the development of another structure unique to the angiosperms; fruit, the subject of next weeks lab. Learning Outcomes: After completing this laboratory, you should be able to: 1. Learn terminology used to describe floral structures of plants 2. Making careful observations and drawings of floral structures 3. Review the differences between monocot and dicot flowers 4. Use dissecting microscopes to examine plant ovaries 5. Review different modes of pollination Bio 103 Spring 2011 1 -1 Flowering Plants Not all plants have flowers, and there was a time in history when no plants at all had flowers. Conifers for example have cones, which produce pollen or seeds, they function as structures for sexual reproduction but they are not flowers. About 125 million years ago, primitive flowers appeared in the fossil record. In addition to producing pollen and eggs, the sex cells of plants, they had adaptations which encouraged other species to carry pollen from the flowers of one plant to another of the same species. Originally beetles were attracted to eat the pollen and inadvertently transferred some of the pollen they had failed to eat to other flowers of the same species, thus achieving pollination. As time progressed flowering plants formed numerous and intricate relationships with a variety of insects including moths, butterflies, flies, and bees, that drove diversification of both plants and insects. In addition to flowers, flowering plants, (Angiosperms) had one more trick up their sleeves; fruit. Following fertilization the ovules develop into seeds, and the ovary grows into a structure with adaptations to facilitate seed dispersal. Various contrivances have evolved, including dehiscent seed pods that rupture scattering seeds around the parent plant, feather-like plumes to catch air currents, barbs and hooks to snag passing hairy beasts, or edible, juicy, and seed packed fruit, eaten and dispersed by a variety of animals. Today flowering plants dominate the world. Floral Structure Most simple flowers consist of four basic whorls of modified leaves: sepals, petals, stamens that produce pollen, and the innermost carpel, the female reproductive organs consisting of the ovary and the stigma, the site of pollination. Following fertilization (an event occurring after pollination) ovules form seeds, while the ovary develops into some form of fruiting structure. Fusion of Floral Parts While some flowers have free petals, many familiar flowers have their petals partially fused to form a tube. The term corolla is used to refer collectively to the petals of a flower be they free or fused. Bio 103 Spring 2011 1 -2 Activity 1: Virtual Herbarium Flower Anatomy Introduction- We will make use of the online illustrated Herbarium to help to learn and visualize key terms used to describe plant floral structures. http://www.vplants.org/plants/glossary/ FLORAL ARRANGEMENT DESCRIPTION Inflorescence catkin corymb Panicle Head/Capitulum Raceme Umbel Bio 103 Spring 2011 1 -3 Floral Symmetry Simple flowers are symmetrical in all directions so that if you draw a line through the flower in any direction one side of the line is a mirror image of the other side, much like a pie. Such flowers are radially symmetrical; lillies and sunflowers and California poppies are examples of this form of symmetry, and are also termed actinomorphic (actin = ray) flowers. Other flowers, often regarded as more advanced in their design, have petals which are dissimilar to each other in structure, and are arranged so that it is only possible to draw one line through the flower (often from top to bottom) and still have two mirror images. Such blooms have bilateral symmetry, and are termed zygomorphic flowers. Plant families with zygomorphic flowers include the Orchidaceae, Lamiaceae (mints), Fabaceae (Beans), and Scrophulariaceae (Figworts) SYMMETRY DESCRIPTION actinomorphic zygomorphic Bio 103 Spring 2011 1 -4 EXAMPLE Actinomorphic Flowers Zygomorphic Flowers Bio 103 Spring 2011 1 -5 . FLORAL STRUCTURE DESCRIPTION Sepal Petal Stamen Stigma Pistil Anther Ovary Corolla Calyx Style Filament Receptacle Bract Bio 103 Spring 2011 1 -6 Ovary Position The position of the ovary relative to the petals is an important characteristic often distinguishing different families of flowering plants. In epigynous flowers the ovary is inferior, or below where the petals attach. In hypogynous flowers, the ovary is above, or superior with respect to the petals. In perigynous flowers a hypanthium, which consists of the fused bases of the sepals, the petals, and the stamens is present, with the sepal and petal lobes separating at the mid-position of the ovary. OVARY DESCRIPTION POSITION Epigynous Perigynous Hypogynous Superior Inferior Locule Bio 103 Spring 2011 1 -7 PLANT TYPES DESCRIPTION Moneocious Dioecious Staminate Flowers Pistilate flowers Unisexual Procedure: Use the definitions above to label the following diagram Bio 103 Spring 2011 1 -8 Composite Flowers. Members of the Sunflower family, the Asteraceae have a dense flower head (also called a capitulum) which consists of either disk florets, ray florets, or both. What appear to be the ‘petals’ radiating out from the edge of the flower are themselves miniature flowers. The individual florets lack peduncles and all attach to a shared receptacle. Many bracts form an involucre under the capitulum. Thes are termed phyllaries, or involucral bacts, and these bear the spines of thistles. ASTERACEAE FLOWERS DESCRIPTION Ray Floret Disk Floret Pappus involucre recepticle Bio 103 Spring 2011 1 -9 Activity 2 : Drawing Specimens In this activity you will examine and draw some flowers. Using the terms you defined in Activity 1 label your diagrams, note down the species name using both the Common name and the latin name using Genus species format. Also record the family name. Provide drawings of each specimen in the spaces provided for each question. For each drawing provide a scale. Monocotyledon flower Common Name Family Genus species Scale __________ Bio 103 Spring 2011 1 -10 Dicotyledon Flower Common Name Family Genus species Scale __________ Bio 103 Spring 2011 1 -11 Composite Flower Common Name Family Genus species Scale __________ Bio 103 Spring 2011 1 -12 Activity 3: Monocots v Dicots Referring to your drawings in Activity 2 complete the following table. Monocotyledon Dicotyledon Number of sepals Number of petals Number of stamen Activity 4: Dissecting Scope In this activity we will make use of the dissecting microscope to examine characteristics of our representative plant specimens. Ovary Procedure: Obtain a flower and locate the ovary. Determine the ovary position, then using the razor blade make a cut across the ovary (transverse section. The ovary may contain a number of internal chambers or locules. Make cross sections of two more ovaries and record your observations in the table below. Inside the ovary are the ovules. These correspond to the eggs of animals and, following fertilization, these will develop into the seeds. The ovary will play some part in the formation of the fruit. Genus Number of Locules Bio 103 Spring 2011 1 -13 Activity 5: Pollination Questions 1.a. List three different ways that pollen could get from the anther to the female part (stigma) of a flower. When the pollen reaches the stigma, it germinates into a pollen tube that grows down the style to the ovary and ovules. Concurrently, two sperm cells are formed by mitosis. The sperm cells move down the pollen tube to the ovule, fertilize the egg and form the endosperm (food) for the new plant. 1.b. How could a plant prevent its sperm from fertilizing its own eggs? 1.c. What advantage is there to preventing self-fertilization? Bio 103 Spring 2011 1 -14