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FORENSIC SCIENCE highest standards or which have made the most significant improvements in science, we found Scientific enquiry (Sc1) at the heart of science work. Teachers in these schools describe not only the increased levels of motivation but also the ways in which Sc1 has promoted learning in the programmes of study for Life processes and living things, Materials and their properties and Physical processes. In the best schools this belief in the significance of Sc1 to learning is underpinned by clear inclusion of Sc1 in schemes of work, in such a way that progression in Sc1 is set out, through planning of science activities, effective sharing of good practice and systematic monitoring of teaching and learning. It is exhilarating to see young people working hard and enjoying what they are doing. Her Majesty’s Inspectorate (HMI) have access to the large amount of evidence gathered through school inspections by Ofsted teams, and this is augmented by visits of HMI to schools. This considerable wealth of data allows us to identify the issues that are impacting on the quality of science education. This work is directed at further improvements in the standards of science education our children receive. I would offer four areas for improvement in primary science education: Making the most of scientific enquiry. Making better use of assessment for learning. Improving the coordination/ leadership of science. Improving teachers’ knowledge and understanding of science. Ian Richardson HMI is the Specialist Adviser for Science with Her Majesty’s Inspectorate. E-mail: [email protected] 6 PRIMARY SCIENCE REVIEW 90 • Nov/Dec 2005 IVOR HICKEY, COLETTE MURPHY, JIM BEGGS AND KAREN CARLISLE DESCRIBE HOW PRIMARY SCHOOL CHILDREN CAN CARRY OUT DNA FINGERPRINTING L ooking at the assembled 10- and 11-year-old children in front of me, I became increasingly convinced that this had been a very bad idea indeed. However, I would need to get the lesson started. I tentatively asked, ‘Does anyone know what the branch of police work that deals with DNA analysis is called?’ Possibly, I thought, one or two of the brightest might be able to attempt an answer. The room erupted into a forest of waving arms and faces that appeared to be crying out ‘Ask me, please!’ Every child in the class knew the answer – forensics – and I realised immediately that I was on to a winner. It was quite clear that the children not only knew about DNA but they were really interested in it and its role in crime detection. They described numerous television series from CSI to The Bill in which DNA extracted from the scene of the crime was used to track down and identify suspects. The level of motivation was higher than I had experienced for any topic I had ever dealt with in a school. My roller-coaster morning was the final phase of a project we had developed with funding from Science Year in conjunction with a number of Belfast primary schools FORENSIC SCIENCE Figure 1 Children get a close-up view of DNA samples for fingerprinting being loaded into gel in which we introduced the process of DNA fingerprinting as part of a wider programme to investigate children’s attitudes to science. Children’s understanding of complex ideas There are two main difficulties in taking DNA fingerprinting into the primary school in any meaningful manner. The first is how to explain what is taking place to the children in a way they will understand, and the second is the problem of equipment. We were able to supply the necessary equipment from our laboratories in college, and devised a scheme in which BEd studentteachers who specialised in science worked in the schools, in conjunction with their tutors. The trainees explained the processes involved and carried out the Only one will match the pattern produced by the DNA found at the ‘scene of crime’. These procedures are obviously too complex for primary-age children to understand. Our problem was to translate the science into a form that was understandable by the children, while still being scientifically accurate. Explanation of enzymes and their ability to digest a specific in the classroom practical demonstration of DNA fingerprinting, using knowledge they had acquired during their science modules. Importantly, however, it was the children who interpreted the data and applied their findings to solving the crime. DNA fingerprinting can be carried out in a number of ways. We based our approach on a DNA fingerprinting kit produced for schools by Bio-Rad, a well known supplier of molecular biological materials to researchers (see website). The kit contains five samples of DNA, one representing DNA extracted from biological material found at the scene of a hypothetical ‘crime’ and four taken from ‘suspects’. The DNA is digested by restriction enzymes and each of the suspects’ samples produces a unique pattern of fragments when separated by gel electrophoresis. sequence of bases in DNA was clearly out of the question. In discussion with our students, we decided to omit the concept of enzyme digestion and simply state that DNA from different people breaks up into fragments of different lengths when being analysed. This was because children understand that DNA differs from person to person, since we differ in eye colour and other inherited traits. The children followed this explanation with ease as they already had a general idea that genes are what make us different form each other. We felt that we would have difficulties finding a meaningful explanation of the process of electrophoresis – where the fragments of DNA are separated by forcing them through a gel by means of an electric current. We were surprised to find that from their understanding of the topic of electricity at key stage 2, children were able to grasp the concept of fragments of DNA moving through a gel in an electric current, and that the smaller fragments move more quickly than large ones. Preparing the ground In our opinion, science teaching is more successful when embedded with other areas of the curriculum, such as literacy. We asked class teachers to prepare the ground for DNA fingerprinting by getting children to develop their own ‘murder scenarios’. The children set about this with great gusto: some classes wrote stories, others developed dramas with children acting the parts of victims and suspects. When the scene had been set in this way, each school was visited by a team of three or four students who briefly outlined the process of DNA fingerprinting. They then carried out a practical demonstration in which the children were able to watch the DNA samples being loaded into an electrophoresis gel, and the apparatus being attached to a power supply. After 45 minutes the power was switched off, and the gels removed. Analysing the DNA evidence from ‘suspects’ The children could then see how dye from the samples had moved into the gel. The DNA remained PRIMARY SCIENCE REVIEW 90 • Nov/Dec 2005 7 FORENSIC SCIENCE invisible at this stage and required overnight staining; by next morning the staining was completed and the results were unveiled. Fortunately our students had performed excellently and in each case the ‘right’ results were obtained! Children were then allowed to examine the gels for themselves. They compared the patterns produced by DNA found at the scene of the crime with those produced by DNA from each of the suspects; nearly all the children picked the correct one. This allowed the scenarios to be completed; the guilty party was known, and the next step was to develop motives. Children set about this with considerable enthusiasm: some of the motives were spine chilling to say the least. One school even went so far as to set up a court scene with judge, jury and defending and prosecuting barristers. Being on the inside of science The responses of the children show that the programme was most definitely a big hit. Why should this be the case? Given that the science content was derived from more advanced syllabuses, it might be thought that the children would have found it rather esoteric and dull. The reason for success appears to be that all the children had knowledge of DNA, developed not from their school curriculum but from television dramas and in some cases news programmes. This allowed them to identify with the process and to enjoy playing the role of detectives. In addition, although the children had learned what DNA fingerprinting can be used for, they had never seen the laboratory processes involved. In a very real sense, our children enjoyed ‘being on the inside of science’ and making the decisions from real data where they had seen the experiment carried out. Including a literacy aspect to the programme only increased their enjoyment by allowing them to link real science data to their imaginary crime scenarios. This type of practical experience helps children to develop their interest in science. The key is not content or process skills, but the experience of amazement, enjoyment and inspiration, as 8 PRIMARY SCIENCE REVIEW 90 • Nov/Dec 2005 demonstrated in the intense concentration of the girl in Figure 1 watching the DNA sample being loaded. In our college training modules for intending primary teachers, practical classes on DNA extraction are always included. These are based on extraction from onions, kiwi or other plant material, using protocols widely available on the Web (see website). The point is to enable children (and often the students too) to experience the ‘wow!’ factor of seeing DNA appear in a test-tube before their very eyes, as a result of their personal manipulation skills. Given the children’s pre-existing interest in DNA fingerprinting, we were surprised to note that in a survey of primary schools in Northern Ireland only a small majority of teachers used the terms DNA or genes even with year 7 (11/ 12 year-olds) classes (Hickey and Quinn, 2002). Admittedly, these terms are not found in the programme of studies, but with the interest in the topic displayed by children it might be valuable to include this sort of topic to stimulate children’s perception of science. DNA is linked to variation in human populations and hence also has a relevance to citizenship education. At a time when there is clear evidence that children are losing their interest in science education (Murphy and Beggs, 2003) it is vitally important that we take advantage of the areas of science that children experience in the world outside school, to fire their imagination and encourage them to take the subject further. How can you do it? Finally, there is the obvious question: ‘How could I run this sort of programme with my class?’ Clearly, even well-funded primary schools are unlikely to be able to afford the apparatus necessary for this type of activity, and not all primary teachers will have the time or inclination to develop the necessary manipulative skills. However, this need not prevent upper primary classes from having this sort of scientific experience. We used college equipment and students to deliver the bulk of the programme with the class teachers. This co-teaching approach is very successful and both students and teachers benefit (Murphy et al., 2004). Many university biology departments are keen to make connections with schools and often have schemes to allow students to obtain experience in classroom situations. And, like us, you may be able to link with a science/discovery centre (in our case, W5 – see website) to stage ‘forensic days’ in which children carry out a full investigation of a hypothetical murder. Here again, undergraduate students proved excellent facilitators; contact your local or partner science teacher educators. Funding for such events can be sought from a number of sources such as the Royal Society, and the BBSRC. So why not introduce your class to solving a murder using modern methods? They will find it much more fun than playing Cluedo! References Hickey, I. and Quinn, C. (2002) Progression of scientific terminology in education. In Proceedings of ATSE Conference, Science within and beyond the National Standards. pp. 22–23. Murphy, C. and Beggs, J. (2003) Children’s perceptions of school science. School Science Review, 84(308), 109–116. Murphy, C., Beggs, J., Greenwood, J. and Carlisle, K. (2004) Students as ‘catalysts’ in the classroom: the impact of co-teaching between science student teachers and primary classroom teachers on children’s enjoyment and learning of science. International Journal of Science Education, 26(8), 1023–1025. Websites DNA fingerprinting kit for schools produced by Bio-Rad: http://biorad.com Protocols for extraction of DNA: http://gslc.genetics.utah.edu/units/ activities/extraction? W5 (WhoWhatWhereWhenWhy): http://www.w5online.co.uk/ Ivor Hickey and Jim Beggs are principal lecturer and head of science respectively at St Mary’s University College, Belfast. Colette Murphy is head of learning and teaching at the Graduate School of Education, Queen’s University, Belfast; Karen Carlisle is her research associate. Email: [email protected]