Download Lab One: Techniques for Better Microscope Use

Name __________________
BioPreAP/GT
Lab One: Techniques for Better Microscope Use
Purpose:
Part A: Microscope Parts and Functions
*Refer to Fig. 1 to refresh your memory on the parts of the microscope.
*The objectives are located on the revolving nosepiece and can be changed by turning
the nosepiece.
1. The eyepiece contains a glass lens which magnifies ____ times (___x).
2. The low power objective (the YELLOW obj.) is _______x.
(which means it magnifies ___times)
3. The high power objective (the BLUE obj.) is ______x
(which means it magnifies ___ times).
Note: The RED (5X) will help you find objects but we consider it “beyond” low. The
YELLOW (10X) will be considered low power.
If you multiply the eyepiece by the objective it will give you the total magnification.
4. The total magnification of an object when viewed under low power is _______x.
5. What is the total magnification of your microscope under high power?_______x.
Refer to the colored direction packets to complete the lab.
Part B: Position of Objects When Viewed With a Microscope
6. _____________________________________
7. _____________________________________
8. _____________________________________
9. _____________________________________
Part C: Use of the Diaphragm
10.
1
2
3
4
5
11.
5
1
2
3
4
Part D. Depth of Field
12. Yes or No
13. Yes or No
Part E: Stains as an Aid to Microscope Work
(
X)
______________
(
X)
______________
Analysis:
True or False
T or F 1. Total magnification of a microscope is determined by adding the eyepiece lens
magnification to the objective lens magnification.
T or F 2. Objects viewed under the microscope appear upside down.
T or F 3. When moving the slide toward the left, objects viewed through the
microscope will move toward the left.
T or F 4. The diaphragm is used to adjust the amount of light entering the microscope.
T or F 5. All objects in different depths appear in focus at the same time while using
high power.
T or F 6. Stains are used to help make clear objects appear lighter under the
microscope.
LAB Two: Comparing Plant and Animal Cells
ONION
(
X)
______________
1.
2.
3.
4.
_____________________________________
Yes or No
_____________________________________
_____________________________________
Label: cell membrane,
cell wall,
nucleus,
cytoplasm
(
X)
______________
5. _____________________________________
6. _____________________________________
7. Yes or No
8. Yes or No
CHEEK CELLS
Label: cell membrane,
cytoplasm,
nucleus
(
X)
______________
9. _____________________________________
Analysis:
1. How does the outer edge of the onion cells differ from the cheek cell? (describe)
2.
a.
What is the outer edge of the onion called?
b.
What is the outer edge of the cheek cell called?
3. What are some structural differences between plant cells and animal cells?
(specific organelles)
4. What is the basic unit of structure in all living things?
5. What advantages do plant cells have over animal cells?