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Antibodies to Human, Animal, and Plant Pathogens BacTrace® Anti-Bacteria antibodies are a powerful set of tools for the detection of human, animal, and plant pathogens. These affinity purified antibodies offer excellent specificity and sensitivity in immunoassays such as ELISA, Western blot, lateral flow, agglutination, flow cytometry, and immunofluorescence. BacTrace polyclonal antibodies can be used as capture or detection antibodies. Often times there is no need for a secondary antibody. You can be sure that a labeled BacTrace antibody is more sensitive than many commercially available monoclonal antibodies. All BacTrace antibodies meet our high standards for potency, minimal cross reactivity, and lot-to-lot consistency, and KPL’s management system is registered to ISO 9001. No matter whether you are interested in antibodies to human, animal, or plant pathogens, KPL will always have the quality you need. BacTrace Antibody Benefits Versatility: BacTrace antibodies are ideal for dual antibody capture and detection assays. Sensitivity: BacTrace polyclonal antibodies are more sensitive than many commercially available monoclonal antibodies. Specificity: BacTrace polyclonal antibodies recognize numerous bacteriaassociated antigens. Reproducibility: Standardized BacTrace antibody lots provide consistent performance. Versatility BacTrace antibodies and conjugates are well suited for most applications from basic detection methods such as sandwich ELISA to more advanced detection methods such as inclusion in biosensors to immunomagnetic enrichment of bacterial cultures. This versatility allows scientists to use antibodies from the same product family for both capture (unlabeled) and detection (labeled) purposes, eliminating the need for finding matched pairs of monoclonal and polyclonal antibodies. Specificity of KPL’s BacTrace STEC Antibodies E. coli O157 Captured with Magnetic Beads A sandwich ELISA was run using KPL’s BacTrace antibodies. Unlabeled BacTrace antibodies were used for capture and corresponding HRP conjugates were used for detection. The results show their superior specificity. E. coli O157 Detected with Anti-E. coli O157 Antibody 0.45 Probe Length (nm) 9.7 x 108 cfu/mL 0.25 9.7 x 107 cfu/mL 0 60 120 180 240 290 Time (seconds) Bio-layer interferometry of E. coli O157 in hamburger extract. Rapid detection (<5 min) of E. coli O157 using a label-free biosensor platform: ForteBio’s Octet Red96. Streptavidin probe tips were coated with KPL’s biotinylated Anti-E. coli O157 before immersion in macerated hamburger supernatents spiked with serial dilutions of E. coli O157. 2 Power Your Immunoassays KPL’s BacTrace Anti-E. coli O157:H7 Magnetic Bead are bound with live, washed E. coli O157:H7 and labeled with FITC Anti-E. coli O157:H7. Bright field and fluorescent images are captured at 100x magnification and overlain to best show both the magnetic bead and the E. coli simultaneously. Sensitivity BacTrace polyclonal antibodies are more sensitive than many commercially available monoclonal antibodies. Because BacTrace antibodies recognize multiple antigens on a given polyantigenic bacterium, these polyclonal antibodies also eliminate concerns over expression of a single given antigen, which may be an issue with monoclonal antibodies. Sensitivity of BacTrace Anti-Legionella Species Antibody vs. Competitor Specificity of BacTrace Anti-Clavibacter michiganensis ssp. michiganensis Antibody Results from an indirect ELISA demonstrating the superior sensitivity of BacTrace Anti-Legionella species polyclonal antibody in detecting heat-killed L. pneumophila as compared to a leading competitor. Specificity of Anti-Streptococcus pyogenes (Group A) An indirect ELISA using KPL’s Anti-Clavibacter michiganensis ssp. michiganensis Antibody to detect CMM and the related CMS. An indirect ELISA using KPL's Anti-Streptococcus (Group A) Antibody to detect various gram-positive and gram-negative bacteria. 1.800.638.3167 www.kpl.com 3 Specificity and Reproducibility BacTrace antibodies are subjected to rigorous purification methods. These purification protocols allow KPL to maintain specificity against the target organism and ensure lot-to-lot consistency of our BacTrace products. Specificity of BacTrace Anti-Staphylococcus aureus Antibody Specificity of BacTrace Anti-E. coli O157:H7 Antibody An indirect ELISA was run using KPL’s Anti-Staphylococcus aureus Antibody to detect various gram-positive and gram negative bacteria. Reproducibility of BacTrace Anti-E. coli O157:H7 Antibody An indirect ELISA was performed showing reactivity of BacTrace Anti-E. coli O157:H7 Antibody. BacTrace Anti-E. coli O157:H7 Antibody is highly selective for the O157:H7 serotype and reacts minimally with other potentially cross-reacting serotypes. KPL tested three lots of BacTrace Anti-E. coli Antibody against a series of bacteria by ELISA. The three lots yielded similar reactivity patterns and demonstrated high correlation lot to lot. 4 Power Your Immunoassays Human Pathogens Within the BacTrace line, KPL offers affinity purified antibodies to a range of pathogens that cause disease in humans. Food-Borne Pathogens KPL leads the food-borne detection market by enabling immunodetection, basic research, and diagnostic test development with an offering of highly specific polyclonal antibodies. These antibodies target the food-borne pathogens E. coli, Salmonella, Listeria, Camplyobacter, Vibrio cholera, and Shigella, all of which cause significant disease and economic cost worldwide. KPL was the first to develop antibodies to the “Big Six” Shiga toxin-producing E. coli (STEC) O26, O111, O121, O45, O145, and O103. STECs are responsible for numerous outbreaks traced to the consumption of contaminated foods and beverages. STECs can cause hemorrhagic colitis and post-diarrheal hemolytic uremic syndrome with potentially deadly outcomes. Communicable Pathogens KPL supports human infectious disease research and detection with a growing offering of BacTrace primary antibodies, including AntiHelicobacter pylori, Anti-Staphylococcus aureus, Anti-Streptococcus pyogenes (Group A), and Anti-Streptococcus agalactiae (Group B) antibodies. These antibodies are powerful tools for the study and detection of diseases, including ulcers, strep throat, and MRSA infections. Zoonotic Pathogens KPL supports research in zoonotic pathogens that ultimately infect humans with antibodies against Borrelia burgdorferi and Yersinia. B. burgdoferi is transmitted by ticks and causes Lyme disease in humans. Yersinia can be transmitted by rodents and is responsible for several high-mortality epidemics throughout human history. Environmental Pathogens Legionella bacteria occur in the natural environment, particularly ponds, creeks, and public water sources. Infection occurs through inhalation of contaminated mist. Legionella is most well known for an outbreak of Legionnaires’ disease among American Legion members in Philadelphia in 1976. Animal Pathogens Renibacterium salmoninarum causes bacterial kidney disease (BKD) in salmonid fish. BKD is a chronic disease that causes mortality in juvenile fish and pre-spawning adults. Renibacterium salmoninarum has a significant impact upon both farmed fish and conservation efforts in wild endangered species. Plant Pathogens KPL offers select purified polyclonal antibodies specific for bacteria of significance in the world of agriculture. Clavibacter michiganensis ssp. michiganensis (CMM) is a destructive plant pathogen that has been known to wipe out entire tomato fields. The CMM BacTrace antibody is highly specific for the tomato plant pathogen but has very little specificity for the related potato pathogen Clavibacter michiganensis ssp. sepedonicus. 1.800.638.3167 www.kpl.com 5 Ordering Information Description Unlabeled HRP Biotin DyLight 488 FITC AP 02-92-93 0.5 mg 05-92-93 0.1 mg Reserve AP Magnetic Beads Positive Controls Anti-Campylobacter species 01-92-93 1.0 mg 04-92-93 0.1 mg Anti-E. coli O103 01-95-93 1.0 mg 04-95-93 0.1 mg 16-95-93 0.5 mg 072-03-95-93 0.5 mg 082-01-95-93 1.0 mL 50-95-93 1 vial 04-95-91 0.1 mg 16-95-91 0.5 mg 072-03-95-91 0.5 mg 082-01-95-91 1.0 mL 50-95-91 1 vial 04-95-95 0.1 mg 16-95-95 0.5 mg 072-03-95-95 0.5 mg 082-01-95-95 1.0 mL 50-95-95 1 vial 04-95-94 0.1 mg 16-95-94 0.5 mg 072-03-95-94 0.5 mg 082-01-95-94 1.0 mL 50-95-94 1 vial 04-95-90 0.1 mg 16-95-90 0.5 mg 082-01-95-90 1.0 mL 50-95-90 1 vial 04-95-92 0.1 mg 16-95-92 0.5 mg 072-03-95-92 0.5 mg 082-01-95-92 1.0 mL 50-95-92 1 vial 04-95-96 0.1 mg 16-95-96 0.5 mg 072-03-95-96 0.5 mg 082-01-95-96 1.0 mL 50-95-96 1 vial 04-90-90 0.1 mg 16-90-90 0.5 mg 02-90-90 0.5 mg 04-91-99 0.1 mg 16-91-99 0.5 mg 02-91-99 0.5 mg 04-90-01 0.1 mg 16-90-01 0.5 mg 072-03-90-01 0.5 mg 50-90-01 1 vial 04-90-02 0.1 mg 16-90-02 0.5 mg 072-03-90-02 0.5 mg 50-90-02 1 vial Human Pathogens Food-Borne Pathogens 50-92-93 1 vial 011-95-93 0.1 mg Anti-E. coli O111 01-95-91 1.0 mg 011-95-91 0.1 mg Anti-E. coli O121 01-95-95 1.0 mg 011-95-95 0.1 mg Anti-E. coli O145 01-95-94 1.0 mg 011-95-94 0.1 mg Anti-E. coli O157:H7 01-95-90 1.0 mg Anti-E. coli O157:H7, Molecular Grade 01-95-90-MG 1.0 mg Anti-E. coli O26 01-95-92 1.0 mg 02-95-90 0.5 mg 05-95-90 0.1 mg 011-95-92 0.1 mg Anti-E. coli O45 01-95-96 1.0 mg 011-95-96 0.1mg Anti-Listeria species 01-90-90 1.0 mg Anti-Listeria species, High Sensitivity 01-90-95 1.0 mg Anti-Salmonella Common Structural Antigen (CSA-1) 01-91-99 1.0 mg Anti-Salmonella (CSA-1), Molecular Grade 05-91-99 0.1 mg 50-90-90 1 vial 082-01-91-99 1.0 mL 50-74-01 1 vial 01-91-99-MG 1.0 mg Anti-Shigella species 01-90-01 1.0 mg Anti-Vibrio cholerae 01-90-50 0.5 mg Anti-Vibrio species 01-90-02 1.0 mg BacTrace is a registered trademark of KPL, Inc. DyLight is a trademark of Thermo Fisher Scientific and its subsidiaries. 6 051-90-90 0.1 mg Power Your Immunoassays Ordering Information Description Unlabeled HRP Biotin DyLight 488 FITC AP Reserve AP Magnetic Beads Positive Controls Human Pathogens (continued) Communicable Pathogens Anti-Helicobacter pylori 01-93-94 1.0 mg Anti-Staphylococcus aureus 01-90-05 1.0 mg 04-93-94 0.1 mg 02-93-94 0.5 mg 50-93-94 1 vial 50-90-05 1 vial 011-90-05 0.1 mg Anti-Streptococcus pyogenes (Group A) 01-90-07 1.0 mg 50-90-07 1 vial 011-90-07 0.1 mg Anti-Streptococcus agalactiae (Group B) 01-90-08 1.0 mg 50-90-08 1 vial 011-90-08 0.1 mg Zoonotic Pathogens Anti-Borrelia burgdorferi 01-97-91 1.0 mg 04-97-91 0.1 mg 02-97-91 0.5 mg 051-97-91 0.1 mg 50-97-91 1 vial Anti-Borrelia species 01-97-92 1.0 mg 04-97-92 0.1 mg 02-97-92 0.5 mg Anti-Yersinia species 01-90-04 1.0 mg 04-90-04 0.1 mg 16-90-04 0.5 mg 072-03-90-04 0.5 mg 50-90-04 1 vial 01-90-03 1.0 mg 04-90-03 0.1 mg 16-90-03 0.5 mg 072-03-90-03 0.5 mg 50-90-03 1 vial 01-96-91 1.0 mg 04-96-91 0.1 mg Environmental Pathogens Anti-Legionella species Animal Pathogens Anti-Renibacterium salmoninarum 02-96-91 0.5 mg 05-96-91 0.1 mg 50-96-91 1 vial Plant Pathogens Anti-Clavibacter michiganensis ssp. michiganensis 01-90-51 1.0 mg 011-90-51 0.1 mg 1.800.638.3167 www.kpl.com 7 Time Ti Time eT Tested, este este es ed d,, P Partner artn ar tnerr P Proven rove ro ven ve n As o one ne o off th tthe he fi firs rst rrsst pr p producers rod rod duccerrs of o ccommercial omme om merc me rcia rc iall an ia anti antibodies tibo ti bodi bo dies di es aagainst gain ga inst st b bacteria, accte teri ria, a, K KPL P h PL has as b been een ee n at tthe he fforefront oreffro or ront on ntt o off b ba bacterial acctter eria ria ial im iimmunommu muno odetection de d ete tect ctio ion io on ffo for or mo m more ore ore re than tha h n two two decades. tw deca de cade ca des. de s. KPL’s s. KPL L’ss BacTrace BaccTr Traacce antibodies anti an tiibodi tibo bo b odi dies e have es hav a e served serv se rvved as as key keyy tools ke tool to olss for ol for life liife fe scientists sciien entiist sttss and aan nd diagnostic diag diag di agnost no n ossttic ic kit kit manufacturers ma anu nufa facttur fact urer ers rs in n food foo ood d pathology paath thol olog ol ogyy and og and infectious infe in fecttio fect ou uss d disease. isea is isea eassee. e. Toda Today To day y we w aare r d re dedicated eed dic icat icat a ed e tto o ad aadvancing vanc va ncin nc in ng tth the he field elld of of b bacterial accteeriial d detection etec et eccti tion on tthrough on hrou hr ou ugh gh a ggrowing rro owi w ng lline ine of in ine o aantibodies nttib bod odie die ies and and iim an immunoassay mmu uno noas oas assa s y ccontrols. co ntro nt rols ro lss. Whether W etthe Wh herr yo yyou u ar are conducting are cond co nduc nd u ti uc ting ng basic bas a ic research res esea earc ea rcch orr developing dev evel ellop pin ingg commercial c m co mm mer erci rci cial iaall kits, kit its, s, KPL s, KPL L can caan n meet meet eeet your yo our ur needs. nee eed dss. To Place an Order or for Customer Service For Technical Service Call: Toll Free 800.638.3167 or 301.948.7755, enter 4. Customer Service representatives are available to receive orders Monday through Friday, 8:00 am – 6:00 pm Eastern Time. Call: Toll Free 800.638.3167 or 301.948.7755, enter 5. Technical Service representatives are available Monday through Friday, 8:30 am – 5:00 pm Eastern Time. Fax: 301.948.0169 Attention: Customer Service Fax: 301.948.0169 Attention: Technical Service E-Mail: [email protected] E-Mail: [email protected] Online: www.kpl.com Write: KPL, Inc. 910 Clopper Road Gaithersburg, MD 20878 USA Attention: Technical Service Write: KPL, Inc. 910 Clopper Road Gaithersburg, MD 20878 USA Attention: Customer Service Federal ID Number: 52-1159597 Small Business Vendor Gaithersburg, MD 20878 Phone: 800.638.3167 Fax: 301.948.0169 www.kpl.com ISO 9001 Registered ML366-04