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Cell Cycle Order of Events KEY 1. Interphase begins the cell cycle. 2. G1 phase begins. 3. The cell grows 4. RNA, proteins, and organelles are made 5. G0 phase begins. 6. The cell rests, but does not divide. 7. The cell passes a restriction point. 8. The cell is now committed to a full round of the cycle. 9. S phase begins. 10. Helicase binds to replication forks on chromosomes. 11. The double helix unwinds. 12. DNA polymerase adds complementary nucleotides from 5’ to 3’. 13. Two DNA double helices are formed, each with one old and one new strand. 14. G2 phase begins. 15. The cell grows 16. M phase begins. 17. Prophase begins. 18. The cell’s nuclear membrane breaks down. 19. Spindle fibers form. 20. Centrioles move to opposite ends of the cell. 21. Metaphase begins. 22. Spindle fibers attach to chromosomes 23. Chromosomes line up at the center of the cell. 24. Anaphase begins. 25. The centromeres break down. 26. Sister chromatids are pulled to opposite ends of the cell. 27. Telophase begins. 28. The nuclear membrane re-forms around chromatids, forming two new nuclei 29. Spindle fibers break down 30. Cytokinesis begins. 31. Cytoplasm divides, while a new cell membrane forms. 32. The cell divides into two daughter cells.