Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Gene regulatory network wikipedia , lookup
Protein adsorption wikipedia , lookup
Endomembrane system wikipedia , lookup
Cell-penetrating peptide wikipedia , lookup
Cryobiology wikipedia , lookup
Proteolysis wikipedia , lookup
Protein–protein interaction wikipedia , lookup
Western blot wikipedia , lookup
Paracrine signalling wikipedia , lookup
Polyclonal B cell response wikipedia , lookup
Two-hybrid screening wikipedia , lookup
RESEARCH ASSOCIATE – Biology (Cell culture and protein production) Bicycle Therapeutics is developing a new class of medicines to treat oncology and other important diseases based on its proprietary bicyclic peptides (Bicycles®) product platform. Bicycle Therapeutics is rapidly advancing towards the clinic with its lead programs using Bicycle Drug Conjugates® (BDCs). Bicycle Therapeutics is collaborating in oncology and other areas to realize the full potential of the technology. Bicycle Therapeutics is headquartered in Cambridge, U.K., with U.S. operations in Cambridge, Massachusetts. For more information, visit: www.bicycletherapeutics.com. We have a new opportunity for a Cell Culture Research Associate This candidate will be primarily responsible for maintaining the Bicycle tissue culture facility and its upkeep. The role will involve culturing a variety of mammalian cells (both adherent and suspension) to produce cells to required timelines and formats to support a diverse range of cell based assays for the wider Biology team. Candidates that have demonstrated previous additional experience in a range of recombinant protein production and purification techniques will be at a significant advantage. You should be self-motivated well organised, diligent in record keeping, have a working knowledge of the drug discovery process and be able to perform consistently at the highest level. Key Responsibilities: • Management of a tissue culture facility to support drug discovery activities o General management of the tissue culture facility including upkeep and scheduling servicing of tissue culture equipment and general maintenance o Resuscitation, maintenance and routine passage of a variety of adherent and suspension cell lines of hazard level 1 and 2 o Preparation of cells for assay in a variety of formats for the Biology team o Manage frozen cell stocks to ensure adequate provision for assays and long term storage in liquid nitrogen o Maintain accurate and systematic records and documentation of procedures • Production, maintenance, and archiving recombinant cell lines for ectopic expression of proteins to produce proteins/antibodies and to conduct various cell based assays • Provision of assay support to both biology teams o Responsibilities include target biotinylation/tagging, bead conjugation and general reagent preparation following defined SOPs o Designing and running basic cell based assays on a variety of assay platforms • Performing routine protein/antibody purification and characterization to support the Biology team o Akta FPLC to purify proteins by IMAC & size exclusion chromatography o QC of purified protein by SDS-PAGE and Western blot • Familiar with working under and drafting Standard Operating Protocols (SOPs) Key Skills and Competencies: • • • • • • • • • A bachelor’s degree in a biological subject or equivalent plus experience of working in a pharmaceutical/biotech setting Significant demonstrable experience in mammalian cell culture and aseptic technique is essential Previous experience in managing a tissue culture facility is highly desirable Experience in transient transfection using lipid-based reagents, recombinant protein production and purification techniques are desirable, although training can be provided Strong interpersonal skills; as a support role, effective interaction with Discovery and Therapeutic Biology teams is essential Good computing skills in MS Office suite software Team player with strong motivational skills Excellent organisational skills and close attention to detail Familiarity with requirements of data integrity and use of Electronic Lab Notebook or similar systems HOW TO APPLY Please send a cover letter and cv to [email protected] with the reference 1701 before the closing date of 7th April