Download Inside this issue - Philippine Plant Variety Protection Office

Volume 2 Issue1
Republic of the Philippines
Department of Agriculture
BUREAU OF PLANT INDUSTRY
PLANT VARIETY PROTECTION OFFICE
Date Released: August 23, 2006
Plant Variety Gazette - Volume 2 Issue 1
Inside this issue
Page
Applications:
Corn
BIOSEED 9909………………………………………………………..
BIOSEED 9888…………………………………………………….….
CZ192…………………………………………………………………
CZ892-nm…………………………………………………………….
CZ280…………………………………………………………………
CY888-nm…………………………………………………………….
PCI001…………………………………………………………………
92P310…………………………………………………………………
DE907…………………………………………………………………
92C112C………………………………………………………………
91P274C………………………………………………………………
01R054………………………………………………………………..
CZ076-nm………………………………………………………….…
CZ172-nm………………………………………………………….…
BIOSEED 9899…………………………………………………….…
Rice
Arize H64…………………………………………………………….
Bitter Gourd
Bonito F1……………………………………………………………..
Notice of Withdrawal of Application for Certificate of Plant Variety
Protection……………………………………………………………………..
Test Guidelines
Corn…………………………………………………………………...
Rice…………………………………………………………………….
Vegetable Legumes
(Cowpea, Yardlong Beans, Bush Sitao)……………………………
Eggplant……………………………………………………………...
Watermelon……………………………………………………….…
Onion and Shallot…………………………………………………...
Tomato……………………………………………………………..…
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Plant Variety Gazette - Volume 2 Issue 1
Application Number
Filing Date
Applicant
Crop
Proposed
Denomination
Applications
: 06-0040
: March 28, 2006
: BIOSEED Research Philippines, Inc. (BRPI)
National Highway, Katangawan, General Santos City
9500 Philippines
: Corn
: 9909 is a modified single cross hybrid
Description of the variety :
a) Leaf Anthocyanin
 Medium (score 5)
b) Foliage
 Light green erect leaves (score 1)
c) Ears
 Medium sized uniform ears completely filled up to the tip
d) Silk
 Light purple
e) Anther color
 Light brown with light coloration at glume base
f) Grain texture
 12-14 kernel rows (some time 16 kernel row also); flat grains semi flint texture
g) Cob color
 White (score 1)
h) Prolific Behavior
 If hybrid is planted spaciously then it bears double ears per plant
i) Disease reaction
 Very good tolerance for Stalk and Ear rots
Filipino Translation
Mga Katangian ng mga Barayti
Ang BIO 9909 ay isang modified single cross hybrid;
a) Anthocyanin sa dahon
 bahagyang matingkad (score 5)
b) Kadahunan
 mapusyaw na berde at tuwid ang mga dahon (score 1)
c) Anther
 mapusyaw ng tsokolate at may bahagyang kulay sa glume base
d) Buhok ng puso
 mapusyaw na ube
e) Prolific Behavior
 nagdadalawang puso bawat halaman kapag malapad ang agwat ng tanim
f) Puso
 Bahagyang kalakihan, halos magkakasinlaki ang mga puso, may 12-14 na
hanay ng butil (16 pa kung minsan).
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Plant Variety Gazette - Volume 2 Issue 1
Applicatons
g) Kulay at kalidad ng butil
 lapad ng mga butil at semi-flint.
h) Kulay ng busil/busal
 puti (score 1)
i) Reaksyon sa mga sakit at peste
 may higit ng mabuting tolerance sa Stalk at Ear rots.
--00-Application Number
Filing Date
Applicant
Crop
Proposed
Denomination
: 06-0041
: March 28, 2006
: BIOSEED Research Philippines, Inc. (BRPI)
National Highway, Katangawan, General Santos City
9500 Philippines
: Corn
: BIOSEED 9888
Description of the variety :
a) Hybrid Type
 9888 is a 3 - way cross hybrid
b) Leaf Anthocyanin
 light (score 5)
c) Foliage
 Dark green slightly curved leaves (score 3)
d) Ears
 Big ear sized fully covered with husk leaves
e) Grain color and Texture
 BIO 9888 has bold orange yellow flint kernel
f) Silk
 Silk emerge as green and once expose to sunlight it will change into slightly
purple color
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Plant Variety Gazette - Volume 2 Issue 1
Applications
g) Anther color
 Brown
h) Grain texture
 12-14 kernel rows; orange yellow flint texture
i) Cob color
 white (score 1)
j) Prolific Behavior
 mostly BIO 9888 produce single ear per plant
k) Disease reaction
 good tolerance for disease and insect pest.
Filipino Translation
Mga Katangian ng mga Barayti
Ang BIO 9888 ay isang 3-way cross hybrid;
a) Anthocyanin sa dahon
 maputla (score 5)
b) Kadahunan
 Matingkad na berde na may bahagyang kurbadong mga dahon (score 3).
c) Anther
 Kulay tsokolate
d) Buhok ng puso
 lumalabas sa puso na kulay berde ngunit nagiging mapusyaw na ube kapag
nasikatan ng araw.
e) Prolificacy
 ang BIO 9888 ay may isang puso kada isang halaman.
f) Puso
 malalaki ang puso na may 12-14 na hanay ng butil at kumpletong natatakpan
ng dahong-balat.
g) Kulay at kalidad ng butyl
 matingkad na magkahalong dilaw at dalandan na flint.
h) Kulay ng busil/busal
 puti (score 1)
i) Reaksyon sa mga sakit at peste
 may mabuting tolerance
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Plant Variety Gazette - Volume 2 Issue 1
Application Number
Filing Date
Applicant
Crop
Proposed
Denomination
Applications
: 06-0042
: March 28, 2006
: BIOSEED Research Philippines, Inc. (BRPI)
National Highway, Katangawan, General Santos City
9500 Philippines
: Corn
: CZ192
Description of the variety :
a) Hybrid Type
 CZ192 is a inbred line
b) Leaf Anthocyanin
 Medium (score 3)
c) Foliage
 Dark green slightly curved or semi erect leaves
d) Ears
 Medium and uniform ears size
e) Silk
 Dark purple
f) Anther color
 Medium anthocyanin coloration of anthers
g) Grain texture
 12 kernel rows sometimes 14 kernel rows
h) Cob color
 white (score 1)
i) Prolific Behavior
 develop the second ear in high field management
j) Disease reaction
 good tolerance for foliar disease
Filipino Translation
Mga Katangian ng mga Barayti
Ang CZ192 ay isang inbred line.
a) Anthocyanin sa dahon
 mapusyaw na kulay ( score 3)
b) Kadahunan
 matingkad na berde at bahagyang kurbadong mga dahon
c) Anther
 mapusyaw na kulay ng anthocyanin sa mga anthers
d) Buhok ng puso
 matingkad na ube
e) Prolificacy
 nagdadalawa ang puso kapag maganda ang pag- aalaga
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Plant Variety Gazette - Volume 2 Issue 1
Applications
f) Puso
 Bahagyang kalakihan at halos magkakasinlaki
g) Kulay at kalidad ng butyl
 12 hanggang 14 kernel na hanay ng butil.
h) Kulay ng busil/busal
 puti (score 1)
i) Reaksyon sa mga sakit at peste
 may mabuting tolerance sa mga sakit ng dahon
--00-Application Number
Filing Date
Applicant
Crop
Proposed
Denomination
: 06-0043
: March 28, 2006
: BIOSEED Research Philippines, Inc. (BRPI)
National Highway, Katangawan, General Santos City
9500 Philippines
: Corn
: CZ892-nm
Description of the variety :
a) Hybrid Type
 CZ892-nm is an inbred line
b) Leaf Anthocyanin
 Anthocyanin coloration of the sheath is medium (score 5)
c) Foliage
 Light green erect leaves (score 1)
d) Ears
 Small conical
e) Silk
 Purple silk (score 7-9)
f) Grain texture
 Yellow semi flint
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Plant Variety Gazette - Volume 2 Issue 1
Applications
g) Cob color
 White
h) Prolific Behavior
 good percentage of producing two ears per plant
i) Disease reaction
 good tolerance for stalk rot
Filipino Translation
Mga Katangian ng mga Barayti
Ang CZ892-nm ay isang inbred line.
a) Anthocyanin
 bahagyang matingkad na anthocyanin sa leafsheath (score 5)
b) Kadahunan
 mapusyaw na berde at tuwid na mga dahon (score 1).
c) Anther
 bahagyang matingkad na kulay ube ang anthers ngunit walang kulay ang
glume base
d) Buhok ng puso
 Matingkad na ube na sakop lahat ang buhok (score 7-9)
e) Prolificacy
 maraming halaman ang nagdadalawang puso bawat halaman.
f) Puso
 maliliit na conical
g) Kulay at kalidad ng butil
 Dilaw na semi- flint
h) Kulay ng busil/busal
 puti (score 1)
i) Reaksyon sa mga sakit at peste
 mabuting tolerance sa stalk rot.
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Plant Variety Gazette - Volume 2 Issue 1
Application Number
Filing Date
Applicant
Crop
Proposed
Denomination
Applications
: 06-0044
: March 28, 2006
: BIOSEED Research Philippines, Inc. (BRPI)
National Highway, Katangawan, General Santos City
9500 Philippines
: Corn
: CZ280
Description of the variety :
a) Hybrid Type
 CZ280 is an inbred line
b) Foliage
 Dark green erect leaves slightly curve (score 3)
c) Ears
 Long ears tapered on tip and some has extended growth on tip
d) Silk
 Medium purple (score 7)
e) Anther color
 Dark brown anther color and coloration at glume base is absent
f) Grain texture
 Yellow orange flint
g) Cob color
 White
h) Prolific Behavior
 no prolification always single ear per plant
i) Anthocyanin on brace roots
 week (score 3)
j) Disease reaction
 excellent tolerance for stalk rot
Filipino Translation
Mga Katangian ng mga Barayti
Ang CZ280 ay isang inbred line.
a) Kadahunan
 matingkad na berde at bahagyang kurbadong mga dahon (score 3).
b) Bulaklak
 bahagyang kalakihang bulaklak na may mahabang katawan at malilit na
sanga
c) Anther
 Matingkad na tsokolate ang kulay ng anther at walang kulay ang glume base
d) Buhok sa puso
 bahagyang matingkad na ube (score 7)
e) Prolificacy
 palaging isang puso bawat halaman
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Plant Variety Gazette - Volume 2 Issue 1
Applications
f) Puso
 Mahahabang puso na makitid sa dulo at may
g) Kulay at kalidad ng butil
 magkahalong dilaw at dalandan na flint
h) Kulay ng busil/busal
 puti (score 1)
i) Anthocyanin sa brace roots
 mapusyaw na kulay (score 3)
j) Reaksyon sa mga sakit at peste
 maasahang tolerance sa stalk rot.
--00-Application Number
Filing Date
Applicant
Crop
Proposed
Denomination
: 06-0045
: March 28, 2006
: BIOSEED Research Philippines, Inc. (BRPI)
National Highway, Katangawan, General Santos City
9500 Philippines
: Corn
: CY888-nm
Description of the variety :
a) Hybrid Type
 CY888-nm is an inbred line
b) Leaf Anthocyanin
 absent (score 1)
c) Foliage
 green leaves strongly curved (score 7)
d) Ears
 Cylindrical ear with good diameter
e) Silk
 whitish green
f) Anther color
 Brown
g) Grain texture
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Plant Variety Gazette - Volume 2 Issue 1
Applications
 orange flint
h) Cob color
 white (score 1)
i) Prolific Behavior
 Single ear per plant
j) Disease reaction
 excellent tolerance for stalk rot
Filipino Translation
Mga Katangian ng mga Barayti
Ang CY888-nm ay isang inbred line
a) Anthocyanin sa dahon
 walang makikitang kulay (score 1)
b) Kadahunan
 berde at matindi ang kurbada ng mga dahon (score 7)
c) Bulaklak (Tassel )
 kaunti ngunit mahahabang sanga ng bulaklak
d) Anther
 kulay tsokolate
e) Buhok ng puso
 berdeng may halong puti
f) Prolificacy
 isang puso bawat halaman
g) Puso
 hugis cylindrical na may katabaan
h) Kulay at kalidad ng butil
 kulay dalandan na flint
i) Kulay ng busil/busal
 puti (score 1)
j) Reaksyon sa mga sakit at peste
 may mabuting tolerance sa mga sakit ng dahon ngunit mahina laban sa stress
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Plant Variety Gazette - Volume 2 Issue 1
Application Number
Filing Date
Applicant
Crop
Proposed
Denomination
Applications
: 06-0046
: March 28, 2006
: BIOSEED Research Philippines, Inc. (BRPI)
National Highway, Katangawan, General Santos City
9500 Philippines
: Corn
: PCI001
Description of the variety :
a) Hybrid Type
 PCI001 is an inbred line
b) Foliage
 Light green erect leaves (score 1)
c) Ears
 Medium ear size
d) Silk
 Light green
e) Anther color
 Light green with light coloration at glume base
f) Grain texture
 Yellow orange flint
g) Cob color
 white
h) Prolific Behavior
 tendency to develop second ear
i) Anthocyanin on brace roots
 week (score 3)
j) Disease reaction
 excellent tolerance for stalk rot
Filipino Translation
Mga Katangian ng mga Barayti
Ang PCI001 ay isang inbred line.
a) Kadahunan
 mapusyaw na berde at tuwid na mga dahon (score 1).
b) Bulaklak
 tuwid ang mga sanga ( score 1)
c) Anther
 mapusyaw na berde at may mapusyaw ding kulay ang glume base
d) Buhok ng puso
 mapusyaw na berde (score 1)
e) Prolificacy
 may kakayahang magbigay ng ikalawang puso
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Plant Variety Gazette - Volume 2 Issue 1
Applications
f) Puso
 May kalakihan
g) Kulay at kalidad ng butil
 magkahalong dilaw at dalandan na flint
h) Kulay ng busil/busal
 puti (score 1)
i) Anthocyanin sa brace roots
 mapusyaw na kulay (score 3)
j) Reaksyon sa mga sakit at peste
 maasahang tolerance sa stalk rot.
--00-Application Number
Filing Date
Applicant
Crop
Proposed
Denomination
: 06-0047
: June 7, 2006
: East-West Seed Company Inc.
Km 54 Cagayan Valley Rd., Bgy. Sampaloc, San Rafael
3008, Bulacan
: Bittergourd, Ampalaya
: Bonito F1
Description of the variety :
“Bonito F1” is a very vigourous and high-yielding native type bittergourd hybrid. The
closest variety is the open-pollinated farmers variety “Ilocos native(OP)”. However, “Bonito
F1 consistently gave higher yield than “Ilocos native(OP)”. Under normal and similar
growing conditions where we compared the two varieties, “Bonito F1” gave and average of
44 fruits per plant while “Ilocos native(OP)” has an average of 20 fruits per plant. Fruits of
“Bonito F1” are slightly longer with an average length of 11 cm compared to 9cm of “Ilocos
native(OP)”. Bonito F1 fruits are more uniform in color (medium green) and in shape
(elongated) as compared to “Ilocos native(OP)” which is variable in color (lightgreen to
medium green) and shape (high round to elongate)
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Plant Variety Gazette - Volume 2 Issue 1
Applications
Filipino Translation
Mga Katangian ng Binhi
Ang Bonito F1 ay isang uri ng native ampalaya hybrid na may napakagandang bulas
ng halaman at nagbibigay ng napakataas na ani. Maikukumpara ito sa open pollinated na
variety na ginagamit ng mga magkakasala na kilala bilang “Ilocos native OP”. Ngunit ang
Bonito F1 ay naiiba sa “Ilocos native OP” dahil ito ay may mas makapal na usbong at
nagbibigay ng higit na mas mataas na ani kumpara sa “Ilocos native OP”. Sa parehong
kondisyon ng pagtatanim kung saan ikinumpara ang dalawang uri, ang Bonito F1 ay
nagbibigay ng mahigit kumulang na 44 bunga kada halaman samantalang ang “Ilocos native
OP” ay nagbibigay lamang na mahigit kumulang na 20 bunga kada halaman. Ang bunga ng
Bonito F1 ay mahaba ng kaunti at may sukat ng labing isa (11) sentimetro kumpara sa siyam
(9) na sentimetrong haba ng “Ilocos native OP”. Kapansin pansin rin sa mga bunga ng Bonito
F1 ang pare-parehong berdeng kulay at pahabang hugis kumpara sa “Ilocos native OP” na
iba-iba ang berdeng kulay at hugis (bilugan hangang pahaba).
--00-Application Number
Filing Date
Applicant
Crop
Proposed
Denomination
: 06-0048
: June 16, 2006
: Tropical Seed Genetics Sciences Incorporation
Macnit, Maladag, Malungon, Sarangani Province
: Corn
: 92P310
Varietal Characteristics :
92P310 is a tall corn inbred line with long droopy light green leaves. It has sparse
tassel with few tassel branches and purple glume. It has prolific tendency. Stalks are big
predominantly green but with shades of purple. It has high density yellow orange flint kernels
of medium size. Under Malandag condition days to silk is 58 days and days to pollen shed is
60 days. Silk is predominantly cream with light purpling of the tip. Root system excellent with
prominent adventitious roots. Husk cover excellent. Produces side cobs. Excellent combine
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Plant Variety Gazette - Volume 2 Issue 1
Applications
Filipino Translation
Mga Katangian ng Binhi
92P310 ay matangkad na klase ng mais na inbred line na may mahabang
nanlupaypay na dahon na kulay hilaw na berde. Ito rin ay may konting tassel na
pinagbubuklod rin ng konting sanga at kulay lila ang glume nito. May kakayahan din itong
magbunga ng marami. Ang tangkay nito ay malalaki na pinatingkad ng kulay nitong berde
na may guhit na lila. Nagtataglay din ito ng marami at magkakadikit-dikit na kulay yellow
orange flint kernels na katamtaman ang laki. Sa Malandag na kalagayan nito lumalabas ang
silk nito limangput walung araw at animnapung araw ang paglabas ng pollen nito. Ang silk
nito ay kulay krema na may halong lila sa dulo nito. Ang mga ugat nito ay napakagaling
dahil sa taglay nitong prominenting adventitious roots. Ang balat ng bunga nito ay maganda
at kaaya-aya. Nagbibigay din ito ing side cobs. At may magandang kakayahan as combiner.
--00-Application Number
Filing Date
Applicant
Crop
Proposed Denomination
: 06-0049
: June 16, 2006
: Tropical Seed Genetics Sciences Incorporation
Macnit, Maladag, Malungon, Sarangani Province
: Corn
: DE907
Varietal Characteristics :
DE907 is a corn inbred line with semi-erect light green leaves. It has medium plant
height and medium size stalks of good quality. It has medium size hard yellow orange flint
kernels of excellent quality. Husk cover is excellent. It is good male having many tassel
branches with good pollen yield. Silk and tassel are purple. It has prolific tendency with
prefix de meaning double ear. It has excellent root system. The line sheds pollen is 53 days
and silks in 54 days under Malandag conditions. It is an excellent combiner.
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Plant Variety Gazette - Volume 2 Issue 1
Applications
Filipino Translation
Mga Katangian ng Binhi
Ang DE907 ay isang mais na inbred line na may medyo nakatayo na dahon na kulay
hilaw na berde. Ito ay may katamtaman ang taas at katamtaman ang sukat ng mga tangkay
nito pero maganda ang kalidad. Nagtataglay din ito ng matigas na butyl na may katamtaman
ang sukat at kulay yellow orange flint kernels na may magandang katangian. Maganda din
ang balat ng bunga nito. Dahil sa taglay nitong maraming tassel at magandang pollen
mainam itong gawing lalaki sa breeding. Ang silk at tassel ay kulay lila. May kapasidad na
magbigay ng tig-dalawang bunga. Maganda ang mga ugat nito. Lumalabas ang pollen nito
limangput tatlong arawat ang silk nito ay limangput apat na araw sa ilalaim ng Malandag na
kalagayan. Ito ay magandang combiner.
--00-Application Number
Filing Date
Applicant
Crop
Proposed Denomination
: 06-0050
: June 16, 2006
: Tropical Seed Genetics Sciences Incorporation
Macnit, Maladag, Malungon, Sarangani Province
: Corn
: 92C112C
Varietal Characteristics :
92C112C is a corn inbred line with narrow semi-erect light green, thick waxy leaves. It
has big stalks of excellent quality. It has short compact tassel that has good pollen yield. It
has medium size ears with excellent tip filling bearing hard flint orange kernels with 16 to 18
kernel rows per ear. Husk cover is short but acceptable. Stay green quality is excellent;
Leaves remain green at harvest. It‟s a late line-days to shed is 62 days and days to silk is 60
days under Malandag, Malungon, Sarangani Province conditions.
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Plant Variety Gazette - Volume 2 Issue 1
Applications
Filipino Translation
Mga Katangian ng Binhi
Ang 92C112C ay isang mais na inbred line na may makitid, medyo nakatayo,
makapal, at makintab na dahon na kulay hilaw na berde. Malalaki ang tangkay nito na
maganda din ang kalidad. Ito ay may maikli at magkadikit-dikit na sanga ng tassel na
nagbibigay ng magandang pollen. Katamtaman lang ang laki ng bunga nito. Matitigas at
kulay dalandann ang butyl nito (flint kernels) na may labinanim hanggang labinwalong
hanay ng butyl sa bawat bunga. Ang balat ng binga ay maikli. Nananatiling kulay berde ang
mag dahon nito kapag inaani. Ito ay isang uri ng line na huli kung mahinog. Lumalabas ang
pollen animnaput dalawang araw at ang silk ay animnapung araw sa Malandag na
kalagayan.
--00-Application Number
Filing Date
Applicant
Crop
Proposed Denomination
: 06-0051
: June 16, 2006
: Tropical Seed Genetics Sciences Incorporation
Macnit, Maladag, Malungon, Sarangani Province
: Corn
: 91P274C
Varietal Characteristics :
91P274C is a corn inbred line of medium plant height with semi-erect light green
leaves. It has big tassel with good pollen yield. Silks and tassel color are predominantly light
purple. It has short ears but with big diameter having 18 kernel rows. It has deep orange
hard flint kernels of excellent quality. Husk cover is good. Stay green excellent. Fair for roots.
Days to silk is 59 days and days to shed is 60 days under Malandag, Malungon, Sarangani
Province conditions.
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Plant Variety Gazette - Volume 2 Issue 1
Applications
Filipino Translation
Mga Katangian ng Binhi
Ang 91P274C ay isang mais na inbred line na may katamtaman ang taas at medyo
nakatayo na dahon na kulay berde. Malalaki ang mga tassel nito na nagbibigay ng
magandang pollen. Ang silk at tassel ay kulay hilaw na lila. Maiikli ang bunga pero malaki
ang diyametro na nagtataglay ng labinwalong hanay. May matigas at magandang uri ng flint
kernels ito kulay dalandan (deep orange). Maganda nag balat ng biunga nito. Nanatiling
berde ang kulay ng kabuuang tanim na ito kahit malapit na itong aanihin. Kainaman ang
ugat nito. Lumalabas ang silk nito limamput siyam na araw sa MAlandag na Kalagayan.
--00-Application Number
Filing Date
Applicant
Crop
Proposed Denomination
: 06-0052
: June 16, 2006
: Tropical Seed Genetics Sciences Incorporation
Macnit, Maladag, Malungon, Sarangani Province
: Corn
: 01R054
Varietal Characteristics :
01R054 is a medium tall corn inbred line with semi droopy leaves. It has excellent root
system with very prominent adventitious roots. It has medium size cylindrical ears on good
husk cover. It has medium size yellow orange flint kernels. It has very big tassels with very
high pollen yield. Weakness is early lower leaf drying due to leaf blight infection. It is an
excellent combiner.
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Plant Variety Gazette - Volume 2 Issue 1
Applications
Filipino Translation
Mga Katangian ng Binhi
01R054 ay isang mais na inbred line na may katamtaman ang taas. Ang mga dahon ay
medyo nanlupaypay. Ang mga ugat nito ay maganda na may prominenteng adventitious na
ugat (prominent adventitious roots). Ang bunga nito ay may katamtaman ang laki na may
magandang balat.Ito ay may katamtaman na butil (flint kernels) na kulay yellow orange.
And tassel ay malaki at maraming pollen. Ang dahon sa ibabang parte ay mapapansin na
madaling matuyo dala ng sakit na leaf blight. Kaaya-ayang combiner.
--00--
Application Number
Filing Date
Applicant
Crop
Proposed Denomination
: 06-0053
: June 27, 2006
: BAYER Crop Science Incorporation
3/F Bayer House, Canlubang Industrial Estate, Canlubang,
Laguna, Philippines
: Rice
: Arize H64
Varietal Characteristics :
Arize H64 hybrid rice, under favorable condition, has potential yield of 7.5 tons per
hectare. It has a maturity range of 95 to 112 days. Approximate plant height is 116 cm. It has
the following grain qualities: 25.49% amylose, G.T. Score of 5.1 I/L/HI, 67.8 Milling Recovery
(Grade 1), 49.5% Headrice (Grade 1). It has a better sensory quality than IR 64, PSB Rc72H
and PSB Rc18.
Arize H64 shows intermediate resistance to Rice Blast and Sheath Blight and shows
low incidence of yellow and white stemborer infestation.
17
Plant Variety Gazette - Volume 2 Issue 1
Applications
Filipino Translation
Mga Katangian ng Binhi
Ang Arize H64 hybrid rice ay may potensyal na ani na 7.5 tonelada bawat ektarya
kapag mainam ang kondisyon. Maari nang anihin ito sa loob ng 95 hanggang 112 araw
pagkatapos ipunla. Tinatantiyang nasa 116 sentimetro ang taas nito.
Ang mga sumusunod ay ang kalidadng butil. 25.49% amylase, G.T. Score na 5.1
I/L/HI, 67.8%, Milling Recovery (Grade 1), 49.5% Headrice (Grade 1) (Grade 2), Mas
malambot at masarap ang lasa kaysa IR64, PSBRc 72H and PSBRc 18.
May katamtamang tibay sa Rice Blast and Sheath Blight (SHB) and Arize.
--00--
Application Number
Filing Date
Applicant
Crop
Proposed Denomination
: 06-0054
: August 9, 2006
: BIOSEED Research Philippines, Inc. (BRPI)
National Highway, Katangawan, General Santos City
9500 Philippines
: Corn
: CZ076-nm
Description of the variety :
a) Hybrid Type
 CZ076-nm is an inbred line
b) Glume base
 1
c) Anthocyanin Coloration of glumes excluding base
 3
d) Silk color
 5
e) Leaf curvature
 3
f) Leaf angle
 3
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Plant Variety Gazette - Volume 2 Issue 1
Applications
g) Stem: degree of zigzag
 1
h) Anthocyanin coloration on leaf sheath covering the upper ear
 1
i) Presence of Second ear
 5
j) Anther color
 3
k) Spikelets density
 3
Filipino Translation
Mga Katangian ng mga Barayti
Ang CZ076 ay isang inbred line.
a) Anthocyanin sa Glume base
 wala (1)
b) Anthocyanin ng Glume na hindi kasali ang puno
 mahina (3)
c) Buhok ng puso
 medyo mahina (5)
d) Kurba ng dahon
 bahagyang kurbado (3)
e) “Angle” ng Dahon
 katamtamang layo (3)
f) Zigzag ng puno
 wala (1)
g) Anthocyanin ng dahong bumabalot sa unang bunga
 wala (1)
h) Pagkakaroon ng pangalawang bunga
 meron (5)
i) Anthocyanin ng Anther
 mahina (3)
j) Density ng spikelets
 katamtaman (3)
k) Bulaklak
 nagkakaroon ng 9-11 sanga
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Plant Variety Gazette - Volume 2 Issue 1
Application Number
Filing Date
Applicant
Crop
Proposed Denomination
Applications
: 06-0055
: August 9, 2006
: BIOSEED Research Philippines, Inc. (BRPI)
National Highway, Katangawan, General Santos City
9500 Philippines
: Corn
: CZ172-nm
Description of the variety :
a) Hybrid Type
 CZ172-nm is an inbred line
b) Glume base
 1
c) Anthocyanin Coloration of glumes excluding base
 1
d) Silk color
 5
e) Leaf curvature
 1
f) Leaf angle
 3
g) Stem: degree of zigzag
 1
h) Anthocyanin coloration on leaf sheath covering the upper ear
 1
i) Presence of Second ear
 5
Filipino Translation
Mga Katangian ng mga Barayti
Ang CZ172-nm ay isang inbred line.
a) Anthocyanin sa Glume base
 wala (1)
b) Anthocyanin ng Glume na hindi kasali ang puno
 wala (1)
c) Buhok ng puso
 medyo mahina (5)
d) Kurba ng dahon
 patayo (1)
e) “Angle” ng Dahon
 katamtamang layo (3)
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Plant Variety Gazette - Volume 2 Issue 1
Applications
f) Zigzag ng puno
 bahagya (3)
g) Anthocyanin ng dahong bumabalot sa unang bunga
 wala (1)
h) Pagkakaroon ng pangalawang bunga
 meron (5)
--00-Application Number
Filing Date
Applicant
Crop
Proposed Denomination
: 06-0056
: August 9, 2006
: BIOSEED Research Philippines, Inc. (BRPI)
National Highway, Katangawan, General Santos City
9500 Philippines
: Corn
: BIOSEED 9899
Description of the variety :
a) Hybrid Type
 BIOSEED 9899 is three way cross yellow corn hybrid
st
b) 1 leaf anthocyanin of sheath
 7
st
c) 1 leaf: Shape of tip
 7
d) Anthocyanin coloration at glume base
 3
e) Anthocyanin Coloration of glumes excluding base
 5
f) Anther color
 3
21
Plant Variety Gazette - Volume 2 Issue 1
Applications
g) Silk color
 5
h) Leaf curvature
 3
i) Leaf angle
 3
j) Anthocyanin coloration on leaf sheath covering the upper ear
 1
Filipino Translation
Mga Katangian ng mga Barayti
Ang BIOSEED 9899 ay isang 3 way hybrid.
a) Anthocyanin ng dahon
 matingkad (7)
b) Hugis ng unang dahon
 ispatula (5)
c) Anthocyanin sa Glume base
 mahina (3)
d) Anthocyanin ng glume na hindi kasali ang puno
 matingkad (5)
e) Anthocyanin ng Anther
 mahina (3)
f) Buhok ng puso
 mahina (5)
g) Kurba ng dahon
 bahagyang kurbado (3)
h) Anthocyanin ng dahong bumabalot sa unang bunga
 wala (1)
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Plant Variety Gazette - Volume 2 Issue 1
NOTICE OF WITHDRAWAL OF APPLICATION FOR
CERTIFICATE OF PLANT VARIETY PROTECTION
The public is informed that „Precious’, a pineapple variety applied for
Certificate of Plant Variety Protection by Mt. Kitranglad AgriDevelopment Corporation on 30 August 2005 was withdrawn on
30 June 2006.
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Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Corn (Zea mays L.)
I. Coverage of These Guidelines
The guidelines for the conduct of distinctness, uniformity and stability tests
(DUS) described herein shall apply to all types of varieties of corn, Zea mays L. (inbred,
single-cross hybrid, double-cross hybrid, three-way cross hybrid and open-pollinated
varieties).
II. Plant Propagation Material Requirement
Upon application, 3,000 viable grains of inbred, hybrid and open pollinated
varieties (OPV) together with the application should be submitted to the Plant Variety
Protection Office (PVPO) as required in RA 9168. Half of the amount will serve as
depository; the rest will be used in the DUS tests. Applicants outside the Philippines
must make sure that all custom requirements regarding seed exportation/importation
are complied with.
The PVP Board will determine the additional quantity of seeds to be submitted
should the need arise. For open-pollinated varieties, three-way and double-cross
hybrids, greater quantities of seeds would be needed since these are genetically more
variable than single-cross hybrids and inbred lines.
Seeds must be of high quality and purity, i.e., high germination capacity (at least
90%), free from any pest or diseases, and high level of genetic purity. The level of
purity will depend on the type of material being applied for protection. Higher level of
genetic purity requirement will be imposed on inbred lines and single-cross hybrids
because these materials are usually more uniform than other types.
The seeds should not have undergone any form of treatment unless otherwise
allowed by the PVP board in certain circumstances. In such cases, the details of the
seed treatment must be clearly described.
III. Conduct of Tests
The minimum duration of the DUS tests should be two similar growing seasons.
The tests should be conducted in at least one accredited DUS Testing Center which
would be determined by the PVP Board. To facilitate the conduct of the tests, the
applicant may submit data from a growing environment similar to that of an accredited
DUS testing center where the tests would be carried out to replace one of the required
growing seasons.
The comparative growing trials must be carried out under optimum growing
conditions recommended for corn. Each trial should have at least two replications in
randomized complete block design. The minimum total number of plants to be tested is
40 for inbred lines and single-cross hybrids, and 80 for other types. For the inbred test,
plot size is 1.5m x 5.0m; each plot consisting of two 5-m rows spaced 0.75 m apart. For
the other types, each plot is 3.0m x 5.0m consisting of four 5-m rows (0.75 apart).
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Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
There should be 26 hills/row with 2 seeds/hill to be thinned out to one
plant/hill one 10-14 days after emergence.
For test of distinctness that involves enzyme electrophoresis, at least 20
coleoptiles should be analyzed. For harmonization with UPOV-member countries, the
procedure for isoenzyme analysis for Zea mays L. (Reference TG/2/6) should be used.
A copy is available at the PVP office upon request.
IV. Methods and Observations
The different characteristics described in the Table of Characteristics (Part VII)
should be used for the testing of distinctness of inbred lines, hybrids and openpollinated varieties. However, to assess the distinctness of hybrids, a prescreening
system based on the parental lines and the hybrid pedigree may be established
according to the following:




the parental line should be described according to the Test Guidelines;
the originality of the parental lines should be checked in comparison
with the reference collection based on the Table of Characteristics in
order to determine the closest inbred line;
the originality of the hybrid pedigree be checked in comparison with
those hybrids which are commonly or publicly known in order to
determine the closest inbred lines;
the distinctness of the hybrids should be assessed with hybrid varieties
of the same formula.
Assessment of distinctness and uniformity should be made on at least 40 plants
or parts of plants excluding outcrossed plants in inbred lines and excluding plants
obviously resulting from the selfing of a parent line in single-cross hybrids. All
observations on the ear should be made on the upper well-developed ear.
For the assessment of uniformity of inbred lines and single-cross hybrids, a
population standard of 3% with an acceptance probability of 95% should be applied.
For instance, in a sample of 40 plants, the maximum number of off-types allowed
would be three (3). The same population standard and acceptance probability should
apply to clear cases of out-crossed plants in inbred lines as well as plants obviously
resulting from the selfing of a parent line in single-cross hybrids (e.g. plant height, size
of ear or earliness as well as proof through enzyme electrophoresis). For three-way
cross hybrids, double-cross hybrids and open-pollinated varieties, the variability within
these varieties should not exceed the variability of comparable varieties already known.
In three-way cross and double-cross hybrids, certain characteristics may
segregate such that several states of trait expression occur side by side within a given
variety. If experience would show that such traits or characteristics are known to
segregate, then such traits or characteristics should be identified with an “S”.
If enzyme electrophoresis is used for testing distinctness, the same population
standard deviation and acceptance probability level as for other characteristics should
be applied. However, a sequential analysis approach could be applied to reduce the
25
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
workload. All inbred lines should be considered out-crosses when two or more loci are
found heterozygous with one allele of the locus of the inbred line (e.g. AX). All cases
where one locus is heterozygous or where two foreign alleles are present should be
considered off-types.
V. Grouping of Varieties
To facilitate the assessment of distinctness, the varieties for evaluation should be
divided into groups. Characteristics which are suitable for grouping purposes are those
which are known from experience not to vary, or to vary only slightly within a variety.
The various states of expression should be fairly evenly distributed throughout the
collection.
It is recommended that the following characteristics be used for grouping
varieties :





time of anthesis (characteristic 5)
anthocyanin coloration of silks (characteristic 14)
plant height (characteristic 20)
number of rows of grain (characteristic 27)
type of grain (characteristic 28)
VI. Characteristics and Symbols
The test for distinctness, uniformity and stability should be based on the
characteristics and their state of expressions as listed in the Table of Characteristics.
Following are the legend-description of the symbols used in the Table of
Characteristics :
(*) Refers to characteristics that should be used on all varieties in every
growing period over which examinations are made and always included in the
variety descriptions, except when the state of expression of a preceding characteristic
of certain environmental conditions would render this impossible
(+) See explanations on the Table of Characteristics in Part VIII.
See explanations on possible segregation in Part IV.
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Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
VII. Table of Characteristics for Maize
Trait
No.
1
2
(+)
Characteristics
Stage1
First leaf:
anthocyanin
coloration of sheath
12 (S)
First leaf: shape of
tip
Description/
Category
absent
Reference
(Example)Varieties
Note/
Remarks
1
weak
3
medium
5
strong
7
pointed
1
pointed to round
2
round
3
round to
spatulate
4
14
5
3
(+)
4
5
(*)
Tassel: anthocyanin
coloration at base
of glume (in middle
third of main axis)
Tassel: anthocyanin
coloration of
glumes excluding
base (as for trait 3)
Tassel: time of
anthesis (on middle
third of main axis,
50% of plants),
DAP
35-50
(S)
spatulate
absent
1
weak
3
medium
5
strong
7
absent
1
weak
3
medium
5
strong
7
very early (<40)
1
early (40-45)
3
medium (45-50)
5
late (50-55)
7
very late (>55)
9
35-50
(S)
40-55
27
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
6
(+)
7
8
(*)
(+)
9
(*)
(+)
10
Characteristics
Stage1
Tassel:
anthocyanin
coloration of
anthers (as for 3; on
fresh anthers)
40-55
Tassel: density of
spikelets (as for 3)
(main axis)
Tassel: angle
between main axis
and lateral
branches (in lower
third of tassel),
degrees
Tassel: curvature of
lateral branches (as
for 8)
Tassel: length of
main axis above
lowest branch, cm
Test Guidelines
Description/
Category
absent
3
medium
5
strong
7
sparse
1
intermediate
3
dense
5
very short (<30)
1
short (30-45)
3
medium (45-60)
5
large (60-90)
7
very large (>90)
9
straight
1
slight curved
3
curved
5
strongly curved
7
very strongly
curved
very short (<30)
9
short (30-35)
3
medium (35-40)
5
long (40-50)
7
very long (>50)
9
50-70
(S)
50-70
Note/
Remarks
1
weak
50-65
50-65
Reference
(Example)Varieties
28
1
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
11
12
(*)
13
14
(*)
15
(+)
Characteristics
Stage1
Tassel: length of
lowest branch, cm
50-70
Tassel: number of
primary branches
Ear: time of silk
emergence (50% of
plants), DAP
Ear: Intensity of
anthocyanin
coloration of silks
Leaf: angle between
blade and stem (on
leaf just above
upper ear), degrees
50-70
45-60
Test Guidelines
Description/
Category
very short
Note/
Remarks
1
short
3
medium
5
long
7
very long
9
absent (0-3)
1
few (3-10)
3
medium (10-15)
5
many (15-20)
7
very many (>20)
9
very early (<45)
1
early (45-50)
3
medium (50-55)
5
late (55-60)
7
very late (>60)
9
absent
1
very weak
3
weak
5
medium
7
strong
9
very small (<30)
1
small (30-45)
3
medium (45-60)
5
large (60-90)
7
very large (>90)
9
45-60
(S)
61
Reference
(Example)Varieties
29
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
16
(-)
17
18
19
20.1
(*)
Characteristic
Leaf: curvature of
blade
(as for trait no 15)
Stem: degree of zigzag
Stem: anthocyanin
coloration of brace
roots
Leaf: anthocyanin
coloration of sheath
covering upper ear
Inbred lines only;
Plant: height (tassel
included), cm
Stage1
61
Test Guidelines
Description/
Category
straight
Note/
Remarks
1
slightly curved
3
curved
5
strongly curved
7
very strongly
curved
9
absent
1
slight
3
strong
5
absent
1
weak
3
medium
5
strong
7
absent
1
very weak
3
weak
5
medium
7
strong
very short (<.100)
9
1
short (100-120)
3
medium (120140)
5
65
65-75
(S)
71 (S)
75
Reference
(Example)Varieties
7
high (140-160)
9
very high (>160)
30
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
20.2
(*)
Characteristics
Stage1
Hybrids and openpollinated varieties
only:
Plant: height
(tassel included),
cm
75
Test Guidelines
Description/
Category
very short (<175)
Reference
(Example)Varieties
Note/
Remarks
1
short (175-190)
3
medium (190-205)
5
high (205-220)
7
very high (>220)
9
21
22
Plant: Height of
uppermost ear to
plant height ratio,
(%)
Leaf: width of
blade
(leaf of upper ear,)
cm
75
75
very low (<30)
1
low (30-45)
3
medium (45-55)
5
high (55-70)
7
very high (>70)
9
very narrow (< 5)
1
narrow ( 5-6)
3
medium ( 6-7 )
5
wide ( 7-8 )
7
very wide (> 8 )
9
23
24
Ear: length of
peduncle, cm
Ear: Anthocyanin
coloration on the
peduncle
85
very short ( < 4)
1
short ( 4- 6)
3
medium ( 6 –8)
5
long ( 8 – 10)
7
very long ( > 10)
9
absent
1
weak
3
strong
5
92
31
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
25
26
27
28
29
Characteristics
Ear: diameter (in
middle), cm
Ear: Number of
husk leaves/ear
Ear: Length of husk
leaves, cm
(1 representative
sample/ear)
Ear: Width of husk,
cm
(in middle, based
on sample in #27)
Ear: shape
Stage1
92
92
92
92
Test Guidelines
Description/
Category
very small (< 3)
Reference
(Example)Varieties
Note/
Remarks
1
small ( 3-4)
3
medium ( 4-5 )
5
large ( 5 –6)
7
very large (> 6)
9
very few (<5)
1
few (5-10)
3
average (11-15)
5
many (16-20)
7
very many (>20)
9
very short (<15)
1
short (16-20)
3
medium (21-25)
5
long (26-30)
7
very long (>30)
9
very short (<5)
1
short (6-10)
3
medium (11-15)
5
long (16-20)
7
very long (>20)
9
Conical
1
conico-cylindrical
3
cylindrical
5
bear paw
7
92
32
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
30
(*)
31
32
33
34
Characteristics
Stage1
Ear: length
(without husk), cm
92
Ear: diameter (in
middle), cm
Ear: Unfilled tip,
cm
Ear: Grain row
arrangement
Ear: number of
rows of grain
92
92
92
92
Test Guidelines
Description/
Category
very short ( < 5 )
Reference
(Example)Varieties
Note/
Remarks
1
short (5 –10 )
3
medium ( 10-15 )
5
long (15 – 20)
7
very long ( > 20)
9
very small (< 3)
1
small ( 3-4)
3
medium ( 4-5 )
5
large ( 5 –6)
7
very large (> 6)
9
Almost none
(< 1 )
1
Short (1- 2)
3
Long (>2)
5
Distinct/Regular
1
Not
distinct/Irregular
9
very few (< 10)
1
few ( 10-12)
3
medium ( 14)
5
many ( 16)
7
very many ( >16)
9
33
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
35
(*)
Characteristics
Ear: type of grain
(in middle third of
ear)
Stage1
92 (S)
Test Guidelines
Description/
Category
flint
Reference
(Example)Varieties
Note/
Remarks
flint-like
intermediate
dent-like
dent
sweet
pop
opaque
36
37
(*)
Ear: White cap on
top of grain, %
coverage on whole
ear
Ear: color of top of
grain (disregarding
white cap, if
present)
waxy
Absent
92
1
Small coverage
(<25)
3
Medium
coverage (25-50)
5
Large coverage
(50-75)
7
Very large
coverage (>75)
9
white
1
yellowish white
2
yellow
3
yellow orange
4
orange
5
red orange
6
red
7
dark red
8
blue black
9
92 (S)
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Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
38
Characteristics
Stage1
Ear: color of dorsal
side of grain
92 (S)
39.
Ear: Shape of grain
40
Ear: intensity of
anthocyanin
coloration of
glumes of cob
Test Guidelines
Description/
Category
white
Reference
(Example)Varieties
Note/
Remarks
1
yellowish white
2
yellow
3
yellow orange
4
orange
5
red orange
6
red
7
dark red
8
blue black
9
absent
1
very weak
3
weak
5
medium
7
strong
9
93
93 (S)
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Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
VIII. Explanations on the Table of Characteristics
The following explanations refer to Trait Numbers marked with the symbol „(+)‟
in the Table of Characteristics (Part VII):
Trait No. 2
First Leaf: shape of tip
1
pointed
2
pointed
to round
3
round
4
round to
spatulate
5
spatulate
Trait No. 8 and Trait No. 15
Leaf and tassle: angle
1
very small
(<30)
2
small
(30-45)
3
medium
(45-60)
4
large
(60-90)
5
very large
(>90)
Trait No. 11 and Trait No. 16
Leaf and tassel: curvature of blade and of lateral branches
1
straight
2
slightly
curved
3
curved
36
4
strongly
curved
5
very strongly
curved
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Trait No. 3
Tassel: anthocyanin coloration at base of glume
Growth Stages of Corn*
5-7 DAP
Emergence
Coleoptile
2 Leaves
11-12
DAP
arly
4 Leaves
17-18
DAP 5-6 Leaves
Early
20-24
Whorl
DAP
Early
Whorl
8 Leaves
25-30
DAP
MidWhorl
9-10 Leaves
32-35
DAP
Mid-Whorl
12 Leaves
L48-49
DAP
Whorl
Tassel
50-52
DAP
Silks
52-58
DAP
Maturity
90-110
DAP
IX. Reference:
UPOV TG/2/6. 1994-1999. Guidelines for the conduct of tests for distinctness,
uniformity and stability for maize (Zea mays L.). International Union for the Protection
of New Varieties of Plants (UPOV). 75 p.
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Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
X. Technical Questionnaire
Reference Number:
(not to be filled in by the applicant)
TECHNICAL QUESTIONNAIRE
(To be completed in connection with an application for plant variety protection)
1. Species:
Zea mays L.
Maize
2. Name and address of applicant:
3. Proposed denomination or breeders reference:
4. Information on origin, maintenance and reproduction of the variety
4.1
Method of breeding/type of material:
(i)
(ii)
(iii)
(iv)
(v)
(vi)
4.2
inbred line
single-cross hybrid
three-way cross hybrid
double-cross hybrid
open-pollinated variety
other (please indicate formula
[
[
[
[
[
[
]
]
]
]
]
]
Other information
5. Characteristics of the variety to be given (the number in brackets refers to the
correspondingcharacteristic in the Test Guidelines; please mark the state of expression which best
corresponds)
Trait
No.
5.2
(16)
5.3a
(22.1)
Reference
(Example) Varieties
Note/
Remarks
Characteristic
Description/Category
Ear: anthocyanin coloration
of silks
65
(S)
absent
present
1[ ]
9[ ]
75
very short
short
medium
1[ ]
3[ ]
5[ ]
In-bred lines only: Plant
length (tassel included)
38
Plant Variety Gazette - Volume 2 Issue 1
5.3b
(22.2)
Hybrids and open
pollinated varieties only:
Plant: length (tassel
included)
5.4
(30)
Ear: type of grain ( in
middle third of ear)
5.5
(33)
6.
Ear: anthocyanin coloration
of glumes of cob
Test Guidelines
long
very long
7[ ]
9[ ]
75
very short
short
medium
long
very long
1[
3[
5[
7[
9[
]
]
]
]
]
92
(S)
flint
flint-like
intermediate
dent-like
dent
sweet
pop
1[
2[
3[
4[
5[
6[
7[
]
]
]
]
]
]
]
93
(S)
absent
present
1[ ]
9[ ]
Similar varieties and differences from these varieties
Denomination of
similar variety
Characteristics in which
the similar variety is
different
State of expression of
similar variety
State of expression of
candidate variety
In the case of identical states of expression of both varieties, please indicate the size of the
difference.
7.
Additional information which may help to distinguish the variety.
a.
Resistance to pests and diseases
b.
Special conditions for the examination of the variety
c.
Other information
end of document
39
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Rice (Oryza sativa, L.)
I. Subject of these Guidelines
These Test Guidelines apply to all varieties of Oryza sativa L.
II. Material Required
The competent authorities decide on the quantity and quality of the plant
material required for testing the variety and when and where it is to be delivered.
Applicants submitting material from a Country other than that in which the testing
takes place must ensure that all customs formalities and pytosanitary requirements are
complied with.
The material is to be supplied in the form of seed.
The minimum quantity of seeds, to be supplied by the applicant, should be:
General
:
500 grams
Hybrid varieties : If requested, an additional 500 grams of seed of
each component (A, B and R parental lines) should be
submitted.
Panicles
: If requested by the competent authority, at least 50
panicles should also be submitted. The panicles should
be well-developed and not affected by any pest or
disease. They should contain a sufficient number of
viable seeds to establish a satisfactory row of plants for
observation.
The seed should meet the minimum requirements for germination rate (at least
85%), analytical purity 1.1%, health and moisture content not greater than 14%. In cases
where the seed is to be stored, the germination capacity should be as high as possible
and should be stated by the applicant.
The seeds should not have undergone any treatment, which would affect the
expression of the characteristics of the variety, unless the competent authorities allow
or request such treatment. If it has been treated, full details of the treatment must be
given.
40
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
III. Method of Examination
 Duration of Tests
The minimum duration of tests should be 2 independent growing cycles
(1 wet and 1 dry).
 Testing Place
The tests should normally be conducted in one place. If any characteristic of
the variety, which are relevant for the examination of DUS, cannot be seen in that place,
the variety may be tested in an additional place.
 Conditions for Conducting the Examination
The tests should be carried out under conditions ensuring satisfactory growth
for the expression of the relevant characteristics of the variety and for the conduct of the
examination.

Characteristics on plants or parts of plants to be selected in a particular way.

Characteristics containing the following notes in the second column of the
Table of Characteristics should be examined as indicated below:

A Leaf: Unless otherwise indicated, all observation on the leaf should be
made on the penultimate leaf.

Timing of the examination
The optimum stage of development for the assessment of each characteristic is
indicated by a number in the second column of the Table of Characteristics. The stages
of development denoted by these numbers are described at the end of Chapter 8.

Type of observation – visual or measurement
The recommended method of observing the characteristics is indicated by the
following key in the second column of the Table of Characteristics :
MG - single measurement of a group of plants or parts of plants
MS - measurement of a number of individual plants or parts of plants
VG - visual assessment by a single observation of a group of plants or parts of
plants
VS - visual assessment by observation of individual plants or parts of plants
41
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
 Test Design

The design of the tests should be such that plants or parts of plants
may be removed for measurement or counting without prejudice to the
observations which must be made up to the end of the growing cycle.

Each test cycle should be designed to result in a total of at least 400
plants for each candidate variety, which should be divided into two or
more replications.

Single panicle- rows if test on panicle-rows are conducted, at least 20
panicle-rows should be observed.
 Number of Plants / Parts of Plants to be Examined
Unless otherwise indicated, all observations made on individual plants or
determined by measurement or counting should be made on at least 10 plants or
parts taken from each of the 10 plants.
 Additional Tests
In cases where morphological characteristics are insufficient to distinguish
candidate variety, additional tests including phenol test on lemma, DNA finger
printing etc. maybe conducted.
IV. Assessment of Distinctness, Uniformity and Stability
 Distinctness

Recommendation
It is of particular importance for users of this Test Guidelines to consult the
General Introduction prior to making decisions regarding distinctness. However,
the following points are provided for elaboration or emphasis in these Test
Guidelines.

Consistent Differences
The minimum duration of tests recommended in section 3.1 reflects, in
general, the need to ensure that any differences in a character are sufficiently
consistent.

Clear Differences
Determining whether a difference between two varieties is cleardepends
on many factors, and should consider, in particular, the type of expression of the
characteristic being examined, i.e. whether it is expressed in a qualitative,
quantitative, or pseudo-qualitative manner.
42
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Therefore, it is important that users of these Test Guidelines are familiar with the
recommendations contained in the General Introduction prior to making decisions regarding
distinctness.
 Uniformity
It is of particular importance for users of these Test Guidelines to consult the
General Introduction prior to making decisions regarding uniformity. However, the
following points are provided for elaboration or emphasis in these Test Guidelines.
 Uniformity assessment by off-types
For the assessment of uniformity of characteristics on the plot as a whole
(visual assessment by a single observation of a group of plants or parts of plants), a
population standard of 0.1% with an acceptance probability of at least 95% should be
applied. In the case of a sample size of 400 plants the maximum number of off-types
allowed would be

Hybrid varieties
For the assessment of uniformity of single hybrid, a population
standard of 1% with an acceptance probability of at least 95% should be applied.
In the case of a sample size of 400 plants the maximum number of off-types
allowed would be 11.

Single panicle-rows
For the assessment of uniformity of characteristics on single paniclerows, plants or parts of plants (visual assessment by observations of a number of
individual panicle-rows, plants or parts of plants), a population standards of 1%
with an acceptance probability of at least 95% should be applied. In the case of a
sample size of 20 panicles the maximum number of aberrant panicle-rows, plants
or parts of plants should not exceed 1.
 Stability
In practice, it is not usual to perform tests of stability that produce results as
certain as those of the testing of distinctness and uniformity. However, experience has
demonstrated that, for many types of variety, when a variety has been shown to be
uniform, it can also be considered to be stable.
Where appropriate, or in cases of doubt, stability may be tested, either by
growing a further generation, or by testing 500 grams seed or plant stock to ensure it
exhibits the same characteristics as those shown by the previous material supplied.
43
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
V. Grouping of Varieties and Organization of the Growing Trial
The selection of varieties of common knowledge to be grown in the trial with
the candidate varieties and the way in which these varieties are divided into groups to
facilitate the assessment of distinctness is aided by the use of grouping characteristics.
Grouping characteristics are those in which the documented states of
expression, even when produced at different locations, can be used, either individually
or in combination with other such characteristics: (a) to select varieties of common
knowledge that can be included from the growing trial used for examination of
distinctness; and (b) to organize the growing trial so that similar varieties are grouped
together.
The following have been agreed as useful grouping characteristics:
Basal leaf: sheath color (characteristic no. 1)
Days of heading (50% of plants with heads) (characteristic no. 18)
Culm length (excluding panicle; excluding floating rice) (characteristic no. 22)
Decorticated grain: length (characteristic no. 49)
Decorticated grain: color (characteristic no. 52)
Endosperm type: (characteristic no. 52)






Guidance for the use of grouping characteristics, in the process of examining
distinctness, is provided through the General Introduction
VI. Introduction to the Table of Characteristics
 Categories of Characteristics

Standard Test Guidelines Characteristics
Standard Test Guidelines characteristics are those which are approved by
UPOV for examination of DUS and from which members of the Union can select
suitable characteristics for their particular circumstances.

Asterisked Characteristics
Asterisked characteristics (denoted by *) are those included in the Test
Guidelines which are important for the International Harmonization of variety
descriptions and should always be examined for DUS and included in the
variety descriptions by all members on the Union, except when the state of
expression of a preceding characteristics or regional environmental conditions
render this inappropriate.
44
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
 States of Expression and Corresponding Notes
States of expression are given for each characteristic to define the characteristic
and to harmonize descriptions. Each state of expression is allocated a corresponding
numerical note for ease of recording of data and for the production and exchange of
the description.
 Types of Expression
An explanation of the types of expression of characteristics (qualitative,
quantitative and pseudo-qualitative) is provided in the General Introduction.
 Reference Varieties
Where appropriate, example varieties are provided to clarify the state of
expression of each characteristic.
 Legend
(*)
(QL)
(QN)
(PQ)
(+)
Asterisked characteristic – see Section 6.1.2
Qualitative characteristic – see Section 6.3
Quantitative characteristic – see Section 6.3
Pseudo-Qualitative characteristic – see section 6.3
See Explanation on the Table of Characteristics on Chapter 8.
Stage of development : see Section 3.3.2
MG-MS-VG-VSb : see Section 3.3.3
45
Plant Variety Gazette - Volume 2 Issue 1
VII.
Test Guidelines
Table of Characteristics
Char
No.
Method of
Examination
1.
30
VS
2.
3.
40
VG
40
VG
a
4.
Characteristics
Example Varieties
Note
Basal leaf: sheath color
green
1
light purple
2
purple lines
3
purple
4
Leaf: intensity of green color
light
3
medium
5
dark
7
Leaf: anthocyanin coloration
absent
1
present
9
40
VG
Leaf: distribution of anthocyanin
coloration
a
tips only
1
2
margins only
3
blotches only
4
uniform
46
Plant Variety Gazette - Volume 2 Issue 1
Char
No.
Method of
Examination
5.
40
VG
a
6.
Test Guidelines
Characteristics
Note
Leaf sheath: anthocyanin coloration
absent
1
present
9
40
VG
Leaf sheath: intensity of
anthocyanin coloration
a
light purple
3
purple Lines
5
purple
7.
Example Varieties
40
VS
7
Leaf: pubescence of blade surface
glabrous
3
intermediate
5
pubescent
7
a
8.
(*)
40
VS
Leaf: anthocyanin coloration of
auricles
1
absent
9
9.
40
VS
present
Leaf: anthocyanin coloration of
collar
a
absent
1
9
10.
(+)
present
Leaf: shape of ligule
VS
a
truncate
3
acute
5
cleft
7
47
Plant Variety Gazette - Volume 2 Issue 1
Char
No.
Method of
Examination
11.
40
VS
12.
Test Guidelines
Characteristics
Example Varieties
Note
Leaf: color of ligule
colorless
1
green
2
light purple
3
purple stripes
4
purple
5
40
MS
Leaf: length of blade (actual
measurement)
a
Short
3
<30 cm
5
Medium
31-60 cm
Long
> 60 cm
7
13.
40
MS
a
14.
(*)
(+)
60
VG
Leaf: width of blade
Narrow
<1 cm
3
Medium
1-2 cm
5
Broad
> 2 cm
7
Flag leaf: attitude of blade (early
observation)
(actual measurement)
1
erect
3
semi-erect
5
horizontal
7
descending
48
Plant Variety Gazette - Volume 2 Issue 1
Char
No.
Method of
Examination
15.
(*)
(+)
90
VG
Test Guidelines
Characteristics
Example Varieties
Note
Flag leaf: attitude of blade (late
observation)
1
erect
3
semi-erect
5
horizontal
descending
16.
17.
(+)
18.
(*)
40
VS
40
VS
55
VG
7
Culm: kneeing ability (for floating
rice only)
absent
1
present
9
Culm: Angle
erect
1
semi-erect
3
open
5
spreading
9
Days of heading (50% of plants
with heads)
1
very early (< 70)
3
early (71-80)
5
medium (81-90)
7
late (91-100)
9
very late (> 101)
49
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Example Varieties
Char
No.
Method of
Examination
Characteristics
19.
(*)
65
VS
Lemma: anthocyanin coloration of
apex
20.
(*)
21.
(+)
22.
(*)
65.
VS
70.
VS
70
VS
Note
absent
1
red
3
light purple
5
purple
7
deep purple
9
Spikelet: color of stigma
white
1
light green
2
yellow
3
light purple
4
purple
5
Culm: thickness
thin (< 5mm)
1
thick (> 5mm)
9
Culm: length (excluding panicle;
excluding floating rice)
very short (< 50 cm)
1
short (51-70 cm)
3
medium (71-90 cm)
5
long (91-110 cm)
7
very long (> 110 cm)
9
50
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Example Varieties
Char
No.
Method of
Examination
Characteristics
23.
(*)
70
VS
Culm: anthocyanin coloration of
nodes
24.
25.
70
VS
70
VS
Note
absent
1
present
9
Culm: intensity of
coloration of nodes
anthocyanin
light purple
3
purple
5
deep purple
7
Culm: anthocyanin coloration of
internodes
3
light gold
5
purple lines
7
26.
(*)
(+)
27.
(*)
(+)
72-90
MS
90
MS
purple
Panicle: length of main axis
very short (< 11cm)
1
short (11-20cm)
3
medium (21-30cm)
5
long (31-40cm)
7
very long (> 40cm)
9
Panicle: curvature of main axis
erect
1
semi-erect
3
drooping
5
deflexed
7
51
Plant Variety Gazette - Volume 2 Issue 1
Char
No.
Method of
Examination
28.
(+)
70
29.
30.
60
60
VS
Test Guidelines
Characteristics
Example Varieties
Note
Panicle: number per plant
few (<8)
3
medium (8-16)
5
many (>16)
7
Panicle: awns
absent
1
present
9
Panicle: color of awns (early
observation)
3
light green
5
red
purple
31.
(*)
70-80
7
Panicle: distribution of awns
tip –awned
1
short & partly awned
3
short & fully awned
5
long & partly awned
7
long & fully awned
9
52
Plant Variety Gazette - Volume 2 Issue 1
Char
No.
Method of
Examination
32.
90
VS
33.
(*)
34.
(+)
60-80
VS
80-90
Test Guidelines
Example Varieties
Characteristics
Note
Panicle: color of Awns
(late observation)
straw
1
yellowish gold
2
brown
3
reddish brown
4
light red
5
red
6
light purple
7
purple
8
black
9
Spikelet: density of pubescence of
lemma
glabrous
1
hairs on lemma keel
3
hairs on upper portion
5
short hairs
7
long hairs ( velvety)
9
Spikelet: apicular color
white
1
straw
2
brown
3
red
4
purple
5
black
6
53
Plant Variety Gazette - Volume 2 Issue 1
Char
No.
Method of
Examination
35.
(+)
90
36.
(+)
37.
(*)
(+)
38.
(+)
90
90
90
VG
Test Guidelines
Characteristics
Example Varieties
Note
Panicle: presence secondary
branching
absent
1
present
9
Panicle: type of secondary
branching
weak
3
strong
5
clustering
7
Panicle: type of branches
compact
3
intermediate
5
open
Panicle: exsertion
7
well exserted
1
moderately-well exserted
3
exserted
5
partly exserted
7
enclosed
9
54
Plant Variety Gazette - Volume 2 Issue 1
Char
No.
Method of
Examination
39.
90
VG
40.
(+)
41.
(+)
42.
92
VG
92
MS
92
MS
Test Guidelines
Example Varieties
Characteristics
Note
Days of maturity
very early (< 100)
1
early (101-110)
3
medium (111-120)
5
late (121-130)
7
very late (>130)
Leaf: senescene
9
early
3
intermediate
5
late
Sterile lemma: Length
7
short (<1.5mm)
1
medium (1.6-2.5mm)
3
long (>2.5 mm but less than the
lemma length
5
extra long (> 2.5 mm but greater
than the lemma length)
7
asymmetrical
9
Sterile lemma: color
straw
1
gold
3
red
purple
5
7
55
Plant Variety Gazette - Volume 2 Issue 1
Char
No.
43.
44.
45.
Test Guidelines
Method of
Examination
Characteristics
92
MS
Grain: weight of 1000 fully
developed grains by 14 % MC
92.
MS
92.
MS
Example Varieties
Note
very low
1
low
3
medium
5
high
7
very high
Grain: length
9
very short (<5.52mm)
1
short (5.52-6.84mm)
3
medium (6.85-8.17mm)
5
long (8.18-9.5mm)
7
very long (>9.5mm)
9
Grain: width
narrow (1.20-1.72mm)
1
medium (1.74-2.36mm)
3
broad (2.37-3.0mm)
5
very broad (>3.0mm)
7
56
Plant Variety Gazette - Volume 2 Issue 1
Char
No.
Method of
Examination
46.
92
VS
47.
(*)
48.
92
92
Test Guidelines
Characteristics
Example Varieties
Note
Lemma and Palea: color
White
0
Straw
1
Gold and gold furrows
2
Brown spots
3
Brown furrow
4
Brown (Tawny)
5
Reddish and light purple
6
Purple spots
7
Purple furrow
8
Purple
9
Black
10
Decorticated grain: length
short (<5.4mm)
1
medium (5.5-6.5mm)
3
long (6.6-7.4mm)
5
very long (> 7.5mm)
7
Decorticated grain: width
narrow (< 1.1mm)
3
medium (1.1-2.49mm)
5
broad (>2.49mm)
7
57
Plant Variety Gazette - Volume 2 Issue 1
Char
No.
Method of
Examination
49.
(*)
(+)
92
50.
(*)
51.
(+)
Example Varieties
Characteristics
Note
Decorticated grain: shape (in lateral
View)
round (<1.5mm)
1
semi-round (1.5-1.99mm)
3
half spindle-shaped (2.-2.49mm)
5
spindle-shaped (2.5-2.99mm)
7
long spindle-shaped (> 2.99 mm)
9
92
92
Test Guidelines
Decorticated grain: color
white
1
light yellow
2
light brown
3
variegated brown
4
dark brown
5
light red
6
red
7
variegated purple
8
purple
9
dark purple/black
10
Endosperm: type
glutinous
1
non-glutinous
3
58
Plant Variety Gazette - Volume 2 Issue 1
Char
No.
Method of
Examination
52.
(+)
92
53.
(+)
54.
(+)
90
90
Test Guidelines
Example Varieties
Characteristics
Note
Endosperm: amylose content (%)
waxy (0-2%)
1
very Low (2.1-10%)
3
low (10.1-20%)
5
intermediate (20.1-25%)
7
high (>25%)
9
Varieties with Endosperm of
Polished grain Non-Waxy only:
Expression of white Core
less than 5%
1
5-10%
3
11-20%
5
21-40%
7
more than 40%
9
Varieties with Endosperm of
Polished Grain Non-Waxy only:
expression of white Belly
less than 5%
1
5-10%
3
11-20%
5
21-40%
7
more than 40%
9
59
Plant Variety Gazette - Volume 2 Issue 1
Char
No.
Method of
Examination
55.
(+)
92
56.
(*)
92
Test Guidelines
Characteristics
Example Varieties
Alkali digestion
low
1
intermediate
3
high intermediate
5
high
7
Decorticated grain: aroma
absent
1
present
9
VIII. Explanations on the Table of Characteristics
Ad 1
Coleoptile : color
Ad 14 Leaf shape of ligule
1
Truncate
2
Acute
3
Cleft
Ad 18y and late observation
1
erect
3
semi-erect
Note
5
horizontal
60
7
reflexed
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Ad 24, 25, 26 Lemma : anthocyanin coloration and 41 : Spikelet : color and tip
L
e
r
m
a
Ad 28 Stem : thickness
At the lowest internode
Ad 33 and 34 Panicle : length of main axis (33) and curvature of main axis (34)
1
erect
3
semi-erect
5
drooping
61
7
deflexed
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Ad 35 Panicle : number per plant
[Explanation to be provided by Korea]
Ad 43 and 44 Panicle : secondary branching
0
absent
1
weak
2
strong
3
clustering
Ad 45 Panicle : attitude of branches
1
erect
3
erect to
semi-erect
5
semi-erect
7
semi-erect to
spreading
62
9
spreading
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Ad 46 Panicle : exsertion
7
partly
exserted
5
exserted
1
well
exserted
Ad 48 Leaf : senescence
The leaves below the flag leaf are observed at the time of harvest for their retention of
greenness. State (3), leaves are dead when the grains have become fully ripened; state (5),
intermediate (there must be 1 leaf which retains its color); state (7), 2 or more leaves retain
their color at maturity.
Ad 49 Sterile lemma : length
The measurement is made on each of the two sterile lemmas.
Ad 54 and 55 Grain : phenol reaction of lemma (54)
Varieties with phenol reaction of lemma present only : Grain : coloration with phenol
(55)
Method of Testing : Place hulls from 10 grains into a petri dish of 5 cm diameter, and
add 5 ml of 1.5% phenol solution; cover the petri dish, and keep at room temperature (not
very cold) for one day.
Ad 58 Decorticated grain : shape (in lateral view)
(see comment from Japan)
round
semi-round
half spindle-shaped
spindle-shaped
long spindle-shaped
length/width
1
2
3
4
5
< 1.50
1.50-1.99
2.00-2.49
2.50-2.99
>3.00
63
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Ad 60 Endosperm : presence of amylose
How to differentiate: By observation, glutinous rice has waxy grains, and nonglutinous rice are non-waxy to transparent with various grades according to the amylose
content of the endosperm. When it is necessary to differentiate glutinous rice and rice with
very low amylose content, chemical analysis is needed.
Note : In general, the amylose content of glutinous rice of pure line variety is 0%
However, many commercial varieties, especially local, traditional varieties may contain 1-4%
of amylose. This is because the waxy gene is recessive, and when outcrossed by ordinary rice
pollen, the endosperm becomes non-glutinous. Also, some methods of testing may result in a
low % of amylose. Research on chemical structure of waxy rice is still in progress in Japan.
Recently various genes (named “dull” genes) for producing semi-waxy rice have been
identified At present, amylose content of those semi-waxy rice is not less than 5% though it is
not sure if further lower amylose lines will be bred in the future.
The three categories can be simply defined by reaction to KI-I solution; glutinous type
endosperm is stained to reddish purple, non-glutinous type to dark blue purple, and
intermediate type to reddish – blue purple.
Intermediate rice is non-glutinous but with very low amylose, the type which is
recently commanding reputation among consumers in East Asian countries.
KI-I solution is prepared by mixing 0.1 % I2 solution and 0.2 % KI solution.
Ad 61 Endosperm : content of amylose
Method ISO 6647 should be used.
Modified from IRRI system by rounding due to variability of data especially in cool
climates, and adding a rank of very high amylose content considering rice in some area of
southern India.
Further discussion may be needed for alternative definition, not using percentage of
amylose, but just describing as very low, low, intermediate, high and very high.
Ad 62 Varieties with endosperm of amylose absent only. Polished grain : expression of
white core
Korea to provide illustration
Ad 63 Varieties with endosperm of amylose absent only. Polished grain : expression of
white belly
Korea to provide illustration
64
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Ad 64 Alkali digestion
Alkali digestion is much easier to test and simpler to observe, and is parallel to results
of testing gelatinization temperature. Notes 1.0-2.5 corresponding to gelatilization
temperature of 74.5-80C notes 3.5-5.4 to 70-74 C and notes 5.5-7 to 55-59 C (Juliano and
Villareal: Grain Quality Evaluation of World Rices, IRRI, 1993)
Ad 65 Decorticated grain : aroma
The main component of the aroma in rice is the 2-acetyl-1pyrroline (AcPy). To
vaporize this chemical, 10 ml. of a 1.7% solution of KOH should be added to 2 gram of
decorticated grains. The aroma, which is similar to that in pop-corn, is released within ten
minutes. The level of expression is determined by reference to the example varieties.
Decimal Code for the Growth Stages of Cereals
2-digit
Code
00
01
02
03
04
05
06
07
08
09
10
11
12
13
14
15
16
17
18
19
General
Description
Germination
Dry seed
Start of
imbibition
Imbibition
complete
Radicle emerged
from caryosis
Coleoptile
emerged
from
caryopsis
Leaf
just
at
coleoptile tip
Seedling growth
First leaf through
coleoptile
First leaf
unfolded
2 leaves unfolded
3 leaves unfolded
4 leaves unfolded
5 leaves unfolded
6 leaves unfolded
7 leaves unfolded
8 leaves unfolded
9 or more leaves
unfolded
Freekes’
Scale
Additional Remarks on Wheat, Barley, Rye, Oats
and Rice
}
}
}
}
1
}
}
}
}
}
}
}
}
Second leaf visible (Less than 1 cm)
50% of laminae unfolded
65
Plant Variety Gazette - Volume 2 Issue 1
2-digit
Code
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
General
Description
Germination
Main shoot only
Main shoot and 1
tiller
Main shoot and 2
tillers
Main shoot and 3
tillers
Main shoot and 4
tillers
Main shoot and 5
tillers
Main shoot and 6
tillers
Main shoot and 7
tillers
Main shoot and 8
tillers
Main shoot and 9
or more tillers
Stem elongation
Pseudo
stem
erection (2)
1st
node
detectable
2nd
node
detectable
3rd detectable
4th node
detectable
5th node
detectable
6th node
detectable
Flag leaf just
visible
Flag leaf
ligule/collar just
visible
Booting
Flag leaf sheath
extending
Boots just visibly
swollen
-
Freekes’
Scale
2
}
}
}
}
}
}
}
}
}
}3
}
}
}
}
}
}
}
4-
5
6}
}
}
7}
}
}
}
}
}
Test Guidelines
Additional Remarks on Wheat, Barley, Rye, Oats
and Rice
In rice: vegetative lag phase
Jointing stage
Above crown nodes
}
}
8
9
Pre-boot stage
In rice: opposite auricle stage
Little enlargement of the inflorescence,
Early-boot stage
}
Mid-boot stage
}
10
66
Plant Variety Gazette - Volume 2 Issue 1
2-digit
Code
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
General
Description
Boots swollen
Flag leaf sheath
opening
First awns visible
Inflorescence
emergence
} Fist spikelet of
}
inflorescence
just
} visible
}
} ¼ of
inflorescence
emerged
}
} ½ of
inflorescence
emerged
}
}
}3/4 of
inflorescence
emerged
}
}
} Emergence of
Inflorescence
} completed
Anthesis
}
} beginning of
anthesis
}
}
}Anthesis half way
}
}
}Anthesis
complete
}
Milk
Freekes’
Scale
}
Test Guidelines
Additional Remarks on Wheat, Barley, Rye, Oats
and Rice
Late-boot stage
}
}
}
}
10.1 In awned forms only
}
}
N}
}
N = non-synchronous crops
}
}
}
S}
N
S = synchronous crops
}
}
}
S
N
}
}
}
S
N
}
}
}
S
N
}
S
}
}
N
}
S
}
}
N
}
S
}
}
N
}
S
10.2
10.3
10.4
10.5
10.51
Not easily detectable in barley
In rice: Usually immediately
Following heading
10.52
10.53
67
Plant Variety Gazette - Volume 2 Issue 1
2-digit
Code
72
73
74
75
General
Description
development
Caryopsis watery
ripe
Early milk
Medium milk
76
-
77
78
79
80
81
83
84
85
Late milk
Dough
development
Early dough
Soft dough
86
87
88
Hard dough
-
89
Ripening
Caryopsis hard
(difficult to
divide by
thumbnail (3)
70
71
90
91
92
93
94
95
96
97
98
Caryopsis hard
(can no longer be
dented
by
thumbnail) (4)
Caryopsis
loosening
in
daytime
Over-ripe, straw
dead
and
collapsing
Seed dormat
Viable
seed
giving
50%
germination
Seed not dormant
Secondary
dormarcy
Freekes’
Scale
Test Guidelines
Additional Remarks on Wheat, Barley, Rye, Oats
and Rice
10.54
}
}
}
}
}
}
]
}
}
}
11.1
} Increase in solids of liquid endosperm
} notable when crushing the caryopsis
} between fingers
Fingernail impression not held
11.
2
}
}
Fingernail impression held, inflorescence
Losing chlorophyll
11.
3
11.
4
In rice: Terminal spikelets ripened.
In rice: 50 % of spikelets ripened
In rice: Over 90% of spikelets ripened (5)
Risk of grain loss by shedding
68
Plant Variety Gazette - Volume 2 Issue 1
2-digit
Code
99
T1
T2
T3
T4
T5
T6
T7
T8
T9
General
Description
induced
Secondary
dormancy lost
Transplanting
and recovery
(rice only)
Uprooting of
seedlings
Rooting
Recovery of
shoots
Resumption of
vegetative
growth
Freekes’
Scale
Test Guidelines
Additional Remarks on Wheat, Barley, Rye, Oats
and Rice
Reproduced from EUCARPIA Bulletin No. 7, 1974, pages 49-52, with the kind
permission of the authors For further information, see J.C. Zadoks, T.T. Chang and C.F.
Konzak, EUCARPIA bulletin No 7, 1974, pages 42-52.
Notes on the Table
(1) Stage of seedling inoculation with rust in the greenhouse
(2) Only applicable to cereals with a prostrate or semi-prostrate early growth habit
(3) Ripeness for binder (ca. 16% water content). Chlorophyll of inflorescence largely lost.
(4) Ripeness for combine harvester (< 16% water content)
(5) Optimum harvest time.
IX. Literature
“T. Matsuo (ed.) (1993-97): “Science of the Rice Plant (volume 1-3)” Nosan
Gyoson Bunka Kyokai (Nobunkyo), Tokyo, Japan.
Vol. 1 Morphology (1993)
Vol. 2 Physiology (1995)
Vol. 3 Genetics (1997)
Indic (1997)
69
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
X. Technical Questionnaire
Reference Number:
(not to be filled in by the applicant)
TECHNICAL QUESTIONNAIRE
To be completed in connection with an application for plant breeders‟ rights
1.
2.
Subject of the Technical Questionnaire
1.1
Latin Name
1.2
Common Name
Applicant
Name
Address
Telephone No.
Fax No.
E-mail address
Breeder (if different from applicant)
70
Plant Variety Gazette - Volume 2 Issue 1
4.
Test Guidelines
Information on the breeding scheme and propagation of the variety
4.1 Breeding Scheme
4.2 Method of Propagating the Variety
4.2.1 Type of material
(a) Line
Male fertile line
[
]
Male sterile line
[
]
(b) Hybrid
(c) other (specify)
In the case of hybrid varieties, the production scheme for the hybrid and for each
component, the information according to the following Chapters 5 to 7 should be provided on a separate
sheet.
Single Hybrid (SH)
(……female parent……) x (….male parent…..)
N.B. In case of use of male sterility system, please indicate the name of the
maintainer line of the parental line.
4.3 other information
71
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
5. Characteristics of the variety to be indicated (the number in brackets refers to the
corresponding characteristics in Test Guidelines; please mark the note which best
corresponding).
Characteristics
5.1 Basal leaf: sheath color
(2)
green
Example Varieties
Note
1
light purple
2
purple lines
3
purple
4
5.2 Leaf: anthocyanin coloration of auricles
(10)
absent
1
present
9
5.3 Time of healing (50% of plants with heads)
very early
1
early
3
medium
5
late
7
Very late
9
5.4 Stem: length (excluding panicle; excluding
(29) floating rice)
very short
1
short
3
medium
5
long
7
very long
9
5.5 Decorticated grain:
(56)
Very short
1
Short
3
Medium
5
72
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Long
7
Very long
9
5.6 Decorticated grain: color
(59)
White
1
Light brown
2
Variegated brown
3
Dark brown
4
Light red
5
Red
6
Variegated purple
7
Purple
8
Dark purple
9
6. Similar varieties and difference from these varieties
Denominations(s) of
variety(ies) similar to
your candidate variety
Characteristic(s) in
which your candidate
variety differs from
the similar variety(ies)
Describe the
expression of the
characteristics(s) for
the similar variety(ies)
(Example)
Plant: Height
e.g. note 3
e.g. short
e.g. 90 cm
7.
Describe the
expression of the
characteristics(s)
for your
candidate variety
Note 7
Tall
130 cm
Additional information which may help in the examination of the variety
7.1 In addition to the information provided in section 5 and 6, are there any additional
characteristics, which may help to distinguish the variety?
7.1.1 Resistance to pest and diseases
Yes
[
]
No
[
]
(if yes, please provide details)
7.1.2 Other
Yes
[
]
No
(if yes, please provide details)
73
[
]
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
7.1.3 Are there any special conditions for growing the variety or conducting the
examination?
Yes
[ ]
No
[
]
7.1.4 If yes, please give details:
7.2 Special conditions for the examination of the variety
7.2
Other information
8. Authorization
(a) Does the variety require prior authorization for please under legislation concerning
the protection of the environmental, human and animal health?
Yes
[
]
No [
]
(b) Has such authorization been obtained?
Yes
[
]
No [
]
If the answer to (b) is yes, please attach a copy of the authorization.
end of document
74
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Vegetable Legumes
Cowpea (Vigna unguiculata)
Yardlong Beans (Vigna unguiculata var. sesquipedalis)
Bush Sitao (Vigna unguiculata x Vigna unguilata var.)
I.
Subject of these Guidelines
This test guideline applies to all varieties of Vigna unquiculata var. sesquipedales
(Yardlong beans); Vigna unguiculata x Vigna unguilata var. sesquipidalis (Bush sitao)
and Vigna radiata (Cowpea).
II.
Material Required
The PVPO decides when, where and in what quantity and quality the seed
required for testing the variety to be delivered. Applicants submitting material must
make sure that all custom formalities are complied with. The minimum quantity of
seed to be supplied by the applicant in one or several samples should be 1000 grams.
The seed should have 90% germination, 10% moisture and 100 % purity.
The plant material must not have undergone any treatment unless the
competent authorities allow or request such treatment. If it has been treated, full
details of the treatment must be given.
III.
Conduct of Tests
 The minimum duration of tests should normally be two similar growing
periods.
 The tests should normally be conducted at one place. If any important
characteristics of the variety cannot be seen at that place, the variety may be
tested at an additional place.
 The tests should be carried out under conditions ensuring normal growth. The
size of the plots should be such that plants or parts of plants may be removed for
measurement and counting without prejudice to the observations which must be
made up to the end of the growing period. As a minimum, each test should
include a total of 60 plants which should be divided between two or more
replicates. Separate plot for observation and for measuring can only be used if
they have been subjected to similar environmental conditions.
 Additional tests for special purposes may be established. Varieties under test
must receive the same treatment for fertilizer, insecticide and or fungicide
application, watering and cultivation.
75
Plant Variety Gazette - Volume 2 Issue 1
IV.
Test Guidelines
Methods and Observations
 All observations determined by measurement or counting should be made on 20
plants or parts of 20 plants.
 For the assessment of uniformity, a population standard of 2% with an
acceptance probability of 95 % should be applied. The maximum number of
off-types allowed would be 3.
 All observations on the growth habit and on the leaf should be made at the time
of full flowering. All observations on the pod should be made at the time of
fresh market maturity. All observations on the seed should be made on dry seed
harvested from the pods. The seed weight should be measured on four samples
of 100 seeds.
When resistance characteristics are used for assessing distinctness, uniformity
and stability, records must be taken under conditions of controlled infection on at
least 20 plants.
V.
Grouping of Varieties
 The collection of varieties to be grown should be divided into groups to facilitate
the assessment of distinctness. Characteristics which are suitable for grouping
purposes are those which are known from experience not to vary, or to vary
only slightly, within a variety. Their various states of expression should be
evenly distributed throughout the collection.
 It is recommended that the competent authorities use maturity in grouping
varieties.
VI.
Characteristics and Symbols
 To assess distinctness, uniformity and stability, the characteristics and their
states is given in the Table of Characteristics should be used.
 Notes (1 to 9) for purposes of electronic data processing, are given opposite the
states of expression for each characteristic.
 Legend
(*) Characteristics that should be used on all varieties in every growing period
over which the examinations are made and always be included in the variety
descriptions, except when the state of expression of a preceding characteristic or
regional environmental conditions render this impossible.
(+) See explanations on the Table of Characteristics in Chapter VII.
(-) See picture
76
Plant Variety Gazette - Volume 2 Issue 1
VII.
Test Guidelines
Table of Characteristics
Trait
No.
1.
2.
3.
4.
5.
Characteristics
Seed: 100 seed
weight (gms)
Description /
Category
Reference (Example)Varities
Yardlong
Bush
Cowpea
Beans
Sitao
Note /
Remarks
small <13
3
medium
13.1 – 17.9
5
large >18
7
circular
1
circular to
elliptical
2
elliptical
3
kidney shaped
4
circular
1
circular to
elliptic
elliptic
2
circular
1
weak
3
medium
5
strong
7
weak
3
Seed width in
cross section (cm)
narrow
3
Seed width in
cross section (cm)
medium
5
broad
7
narrow
3
Seed shape of
median
longitudinal
section (+)
Seed shape of
median cross
section
Seed shape of
median cross
section
Seed degree of
curvature for
kidney shaped
seeds only
Seed degree of
curvature for
kidney shaped
seeds only
3
77
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
6.
(*)
7.
8.
9.
10.
11.
Characteristics
Seed length
median
Seed number of
colors
Seed main color
(largest area for 2
or more colors)
Seed predominant
secondary color
Seed distribution
of predominant
secondary color
Seed color of hilar
ring (-)
Description /
Category
Test Guidelines
Reference (Example)Varities
Yardlong
Bush
Cowpea
Beans
Sitao
Note /
Remarks
short
3
medium
5
long
7
one
1
two
2
more than two
3
white
1
brown
2
black
3
maroon
4
white
1
brown
2
black
3
around hilum
1
in streaks
2
on half of grain
3
in patches at
tip
4
same color of
seed
not the same
1
2
78
Plant Variety Gazette - Volume 2 Issue 1
Trait
No .
12.
13.
14.
15.
16.
17.
18.
Characteristics
Seed veining
Seed surface
texture
Seed number per
pod
Plant:
anthocyanin
coloration of
hypocotyls (10
days after
emergence)
Plant: branching
habit
Plant: maturity
(number of days
to 1st
harvest/maturity)
Plant: number of
days to 50%
flowering
Description /
Category
Test Guidelines
Reference (Example)Varities
Yardlong
Bush
Cowpea
Beans
Sitao
Note /
Remarks
Weak
3
Medium
5
Strong
7
Smooth
1
Wrinkled
2
Low <13
3
Medium 14-17
5
High >18
7
Absent
1
Present
9
Conical
1
Cylindrical
2
Early
3
Medium
5
Late
7
Early
3
Medium
5
Late
7
79
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
19.
20.
21.
22.
23.
Characteristics
Plant: first pod
bearing node (*)
(number starting
on unfoliate node)
Plant: height
(cms) of the first
fruiting (*) node,
average of 5
plants
Leaf: color
Leaf: petiole color
Leaf: basal petiole
color
Description /
Category
Test Guidelines
Reference (Example)Varities
Yardlong
Bush
Cowpea
Beans
Sitao
Note /
Remarks
Low
3
Medium
5
High
7
Low
3
Medium
5
High
7
Light green
1
Green
2
Dark green
3
Greenish
purple
4
Others
9
Light green
1
Purple
2
Greenish
purple
3
Dark purple
4
Green
1
Purple
2
Dark purple
3
80
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
24.
25.
26.
27.A
27.B
Characteristics
Leaf: petiole
length (at 50%
flowering) (-)
Leaf: leaflet shape
(+) (-)
Leaf: shape of leaf
apex (+) (-)
Leaf: shape of leaf
base (+) (-)
(Center leaf)
Leaf: shape of leaf
base
(Two sides leaves)
Description /
Category
Test Guidelines
Reference (Example)Varities
Yardlong
Bush
Cowpea
Beans
Sitao
Note /
Remarks
Small (<15 cms)
3
Medium (15.1
to 18 cms)
5
Large (>18.1
cms)
7
Deltoid
1
Ovate
2
Ovatelanceolate
3
Lanceolate
4
Longlanceolate
5
Acute
1
Acuminate
2
Caudate
3
Aristate
4
Acute
1
Cuneate
2
Truncate
3
Acute
Cuneate
Oblique
81
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
28.
29.
30.
31.
32.
33.
Characteristics
Leaf: shape of
leaf
margin (+)
Leaf: venation of
the leaf blade (+)
Flower: color of
calyx
Flower: color of
wings
(at full bloom)
Flower: color of
standard
Flower: color of
keel
Description /
Category
Test Guidelines
Reference (Example)Varities
Yardlong
Bush
Cowpea
Beans
Sitao
Note /
Remarks
Entire
1
Undulate
2
Parallel
1
Pinnate
2
Palmate
3
Green
1
Greenish
purple
Purple
2
White
1
Light purple
2
Blue
3
Dark purple
4
White
1
Light purple
2
Blue
3
Purple
4
White
1
Light purple
2
Blue
3
Dark purple
4
3
82
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
34.
35.
(+)
36.
(+)
37.
Characteristics
Pod: length of
peduncle at first
harvest
Pod: length in
cms (mean of 10
pods at second
harvest)
Pod: width in cms
(mean of 10 pods
at second harvest)
Pod: shape of
cross section (+)
Description /
Category
Test Guidelines
Reference (Example)Varities
Yardlong
Bush Sitao Cowpea
Beans
Note /
Remarks
Short
(<20 cms)
Medium
(21 to 27 cms)
1
Long
(>27.1 cms)
9
Very short
(<29)
Short
(30 – 39)
1
Medium
(49 – 49)
5
Long
(50 – 59)
7
Very long
(>60)
9
Narrow
3
Medium
5
broad
7
Very flat
1
Pear shaped
2
Round elliptic
3
Figure of eight
4
5
3
83
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
38.
39.
40.
41.
42.
Characteristics
Pod: ground
color at mature
stage (fresh
pod)
Pod: wall fiber
(taken at dry
pod maturity
(-)
Pod: beak
position (+) (-)
Pod: beak
orientation (+)
(-)
Pod: beak color
Description /
Category
Test Guidelines
Reference (Example)Varities
Yardlong
Bush Sitao
Cowpea
Beans
Note /
Remarks
Light green
1
Dark green
2
Green
3
Light green with
purple shade
4
Purple
5
Fleshy type
(fiber adheres to
dry seeds
1
Leathery podded
2
Excessive
shattering
3
Marginal
1
Non-marginal
2
Upward
1
Straight
2
Downward
3
Green
1
Red
2
Purple
3
Others
4
84
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
43.
44.
45.
46.
47.
48.
49.
50.
Characteristics
Pod: secondary
color
Description /
Category
Test Guidelines
Reference (Example)Varities
Yardlong
Bush Sitao
Cowpea
Beans
Note /
Remarks
Absent
1
Present
9
Pod: hue of
secondary color
(-)
Cream
1
Violet
2
Pod:
stringiness (+)
(-)
Absent
1
Present
9
Acute
1
Acute to truncate
2
Truncate
3
Short
3
Medium
5
Long
7
Smooth
3
Medium rough
5
Rough
7
Absent
3
Medium
5
Pronounced
7
Short
3
Medium
5
Long
7
Pod: shape of
distal part
(excluding
beak) (-)
Pod: length of
beak
Pod: texture of
surface (-)
Pod:
constrictions
(immature
stage)
Pod: shelf life
85
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
51.
51.1
51.2
51.3
51.4
Characteristics
Resistance to
Diseases
Nematode
Cowpea rust
Pole sitao
mosaic virus
Fusarium wilt
52.
Resistance to
Pests
52.1
Pod borer,
Heliothis
armegiera
52.2
52.3
52.4
52.5
53.
53.1
53.2
Description /
Category
Test Guidelines
Reference (Example)Varities
Yardlong
Bush Sitao
Cowpea
Beans
Note /
Remarks
Absent
1
Present
9
Absent
1
Present
9
Absent
1
Present
9
Absent
1
Present
9
Absent
1
Present
9
Pod borer,
Maruca
testulalis
Absent
1
Present
9
Black aphids
Absent
1
Present
9
Absent
1
Present
9
Absent
1
Present
9
Absent
1
Present
9
Absent
1
Present
9
Weevils
Bean fly
Stress
resistance
Water logging
Drought
86
Plant Variety Gazette - Volume 2 Issue 1
VIII.
Test Guidelines
Explanation on the Table of Characteristics :
9.
Leaflet shape:
1
Ovate
10.
2
Deltoid
2
Acuminate
3
Caudate
5
Long-Lanceolate
4
Aristate
Shape of leaf bases:
1
Acute
12.
4
Lanceolate
Shape of leaf apeces:
1
Acute
11.
3
Ovate-Lanceolate
2
Cuneate
Shape of leaf margin:
1
Entire
2
Undulate
87
3
Truncate
Plant Variety Gazette - Volume 2 Issue 1
13.
Venation of the leaf blade:
1
Parallel
22.
2
Pinnate
3
Palmante
Pod cross section:
1
Very flat
26.
Test Guidelines
2
Pear shape
3
Round elliptic
Pod beak position:
1
Marginal
27.
2
Non-marginal
Pod beak orientation:
1
Upward
2
Straight
3
Downward
88
4
Figure of eight
Plant Variety Gazette - Volume 2 Issue 1
38.
Seed shape of median longitudinal section
1
Circular
IX.
Test Guidelines
2
Circular to elliptical
3
Elliptical
4
Kindney-shaped
Literature
 Anonymous, 1931: “Beans of New York” in “Vegetables of New York, “Vol. I,
Part 2 (Hedrick, U.P., a.o.), State of New York Educational Department , pp. 110
 DRIJFHOUT, E., 1978: “Genetic interaction between Phaseolus vulgaris and
bean common mosaic virus with implication for strain identification and breeding
for resistance, “Agricultural Research Report 872, Centre for Agricultural
Publishing and Documentation, Wageningen, NL
 UPOV Guidelines for the conduct of Tests for Distinctness, uniformity and
stability for
French Bean, (Phaseolus vulgaris L.)
 Calingasan, M.K. (2002). Introduction, evaluation and selection on Pole Sitao
in BPI-Los
Baños National Crop Research and Development Center 2002 Annual Report pp.
67-76
 Calibo, Juanita G. (2002). Acquisition, conservation and
utilization of
tomato, eggplant, yardlong beans, squash, vegetable soybeans and mungbeans in
BPI-Los Baños National Crop Research and Development Center 2002 Annual
Report pp. 19-35.
89
Plant Variety Gazette - Volume 2 Issue 1
X.
Test Guidelines
Technical Questionnaire
Reference Number:
(not to be filled in by the applicant)
TECHNICAL QUESTIONNAIRE
to be completed in connection with an application for plant breeders‟ rights
1. Species :
Yardlong beans, Vigna unquiculata variety sesquipedalis
Bush sitao, Vigna unguiculata x Vigna unguiculata var. sesq
Cowpea, Vigna unguiculata
2. Applicant (Name and address)
3. Proposed denomination or breeder‟s reference
4. Information on origin, maintenance and reproduction of the variety
5. Characteristics of the variety to be indicated (the number in brackets refers to the
corresponding characteristic in the Test Guidelines; please mark the state of expression
which best corresponds)
Characteristics
Description/Category
5.3
Number of days to
50% flowering
Early
Medium
Late
5.35
Pod length (cm)
Very short
Short
Medium
Long
Very long
5.38
Pod ground color
5.39
Pod wall fiber
Green
Purple
Fleshy
Leathery
Excessive shattering
Example Variety
Note
1( )
5( )
9( )
< 29
30-39
40-49
50-59
> 60
90
1
3
5
7
9
1
2
1
2
3
Plant Variety Gazette - Volume 2 Issue 1
Characteristics
Test Guidelines
Description/Category
Example Variety
Note
5.3
Number of days to
50% flowering
Early
Medium
Late
5.35
Pod length (cm)
5.38
Pod ground color
5.39
Pod wall fiber
5.45
Pod stringiness
Very short
< 29
Short
30-39
Medium
40-49
Long
50-59
Very long
> 60
Green
Purple
Fleshy
Leathery
Excessive shattering
Absent
Present
1
3
5
7
9
1
2
1
2
3
1
2
5.1
100 seed weight (g)
5.8
Seed main color
Light
Medium
Heavy
White
Brown
Black
Other
Low
Medium
High
3
5
9
1
2
3
4
3
5
9
5.14
Seed number per
pod
1( )
5( )
9( )
< 13
13.1-17.9
> 18
<13
14-17
18 >
6. Similar varieties and differences from these varieties
Denomination of
similar variety
Characteristics in which
the similar variety is
different
State of expression
of similar variety
State of expression
of candidate variety
Note : In case of identical state of expression of both varieties, please indicate the size of the difference
91
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
7. Additional information which may help to distinguish the variety
7.1
Resistance to pests and diseases :
Diseases
Nematode
Cowpea rust
Pole sitao mosaic virus
Fusarium wilt
Pests
Pod borer, Heliothis armigera
Pod borer, Maruka testulalis
Black aphids
Bean fly
Absent
[ ]
[ ]
[ ]
[ ]
[ ]
[
[
[
[
[
]
]
]
]
]
Present
[ ]
[ ]
[ ]
[ ]
[ ]
[
[
[
[
[
]
]
]
]
]
Not tested
[ ]
[ ]
[ ]
[ ]
[ ]
[
[
[
[
[
]
]
]
]
]
7.2 Special conditions for the examination of the variety
( I ) Type of culture
 under glass
 open field
( ii ) Part mainly consumed
 pod
 seed
( iii ) Main use
 fresh market or garden
 canning (indicate type)
( iv ) Other conditions
[ ]
[ ]
[ ]
[ ]
[ ]
[ ]
7.3 Other information
end of document
92
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Eggplant (Solanum melongena L.)
I.
Subject of these Guidelines
These Test Guidelines apply to all varieties of Solanum melongena L.
II.
Material Required
The competent authorities decide when, where and in what quantity and
quality the seed required for testing the variety is to be delivered. Applicants
submitting material from a State other than that in which the testing takes place must
make sure that all customs formalities are complied with. The minimum quantity of
plant material to be supplied by the applicant in one or several samples should be :
15 g.
The quantity of the seed to be delivered should not be below the standards of
seeds for certification or marketing in the country concerned, especially in regard to
germination capacity and moisture content.
The plant material must not have undergone any treatment unless the
competent authorities allow or request such treatment. If it has been treated, full
details of the treatment must be given.
III.
Conduct of Tests

The minimum duration of tests should be two similar growing periods.

The tests should normally be conducted at one place. If any important
characteristics of the variety cannot be seen at that place, the variety may be
tested at an additional place.

The tests should carried out under conditions ensuring normal growth. The
size of the plots should be such that plants or parts of plants may be removed
for measurement and counting without prejudice to the observations which
must be made up to the end of the growing period. As a minimum, each test
should include a total of 20 plants. Separate plots for observation and for
measuring can only be used if they have been subject to similar environmental
conditions.

Additional tests for special purposes may be established.
93
Plant Variety Gazette - Volume 2 Issue 1
IV.
V.
Methods and Observations

All observations determined by measurement or counting should be made on
20 plants or parts of 20 plants.

All observations on the leaf should be made on flowering branches at the early
flowering stage.

All observations on the fruits should be made on normally developed fruits
during the 2nd-3rd harvest period.
Grouping of Varieties

The collection to be grown should be divided into groups to facilitate the
assessment of distinctness. Characteristics which are suitable for grouping
purposes are those which are known from experience not to vary or to vary
only slightly, within a variety and which in their various states are fairly
evenly distributed within the collection.

It is recommended that the competent authorities used the following
characteristics for grouping varieties:






VI.
Test Guidelines
Fruit: length (characteristic 17)
Fruit: ratio length/diameter (characteristic 19)
Fruit: general shape (characteristic 20)
Fruit: color of skin at commercial harvesting (characteristic 25)
Fruit: stripes (characteristic 27)
Fruit: color of flesh (characteristic 38)
Characteristics and symbols

To assess distinctness, homogeneity and stability, the characteristics in the
Table of Characteristics should be used.

Notes (1 to 9), for the purposes of electronic data processing, are given
opposite the states of the different characteristics.

Legend:
(*)
Characteristics that should be used every growing period for the
examinations of all varieties and should always be included in the description of the
variety, except when the states of expression of a proceeding characteristics or
regional environmental conditions render this impossible.
(+) See explanations of the Table of Characteristics in Chapter VIII.
94
Plant Variety Gazette - Volume 2 Issue 1
VII.
Test Guidelines
Table of Characteristics
Traits
1.
(*)
2.
3.
(*)
4.
5.
6.
(*)
7.
8.
Characteristics
Seedling:
anthocyanin
coloration of
hypocotyls
Seedling: intensity
of anthocyanin
coloration of
hypocotyls
Plant:
attitude/growth
habit
Plant: height
Length of stem
from cotyledon to
node of first
flower)
Stem: anthocyanin
coloration
Stem: intensity of
anthocyanin
coloration
Stem: hairiness
absent
Note/
Remarks
1
present
9
weak
3
medium
5
strong
7
erect
3
semi-erect
5
prostrate/spreading
7
hort
3
medium
5
tall
7
short
3
medium
5
long
7
absent
1
present
9
weak
3
medium
5
strong
7
weak
3
medium
5
strong
7
Description/Category
95
Reference
(Example)Varieties
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Traits
Characteristics
Description/Category
9.
Branch: length of
internodes
short
Note/
Remarks
3
medium
5
long
7
small
3
medium
5
large
7
entire
1
dentate
2
sinuate
3
weak
3
medium
5
strong
7
absent
1
present
9
strong
7
absent or very weak
1
weak
3
medium
5
strong
7
very strong
9
green
1
bluish green
2
violet green
3
10.
11.
(*)
(+)
12.
13.
14.
15.
(*)
Leaf: size
Leaf: margin
Leaf: degree of
sinuation of margin
Leaf: blistering
Leaf: spininess
Leaf: color
96
Reference
(Example)Varieties
Plant Variety Gazette - Volume 2 Issue 1
Traits
16.
17.
(*)
18.
19.
(*)
20.
(*)
(+)
21.
(*)
22.
(*)
(+)
Characteristics
Flower: purple
color
Fruit: length
Fruit: diameter
Fruit: ratio
length/diameter
Fruit: general shape
Fruit: size of pistil
scar
Fruit: shape of apex
Test Guidelines
light
Note/
Remarks
3
medium
5
dark
7
short
3
medium
5
long
7
small
3
medium
5
large
7
very small
1
small
3
medium
5
large
7
very large
9
pear-shaped
1
ovoid
2
globular
3
cylindrical
4
small
3
medium
5
large
7
indented
1
rounded
2
pointed
3
Description/Category
97
Reference
(Example)Varieties
Plant Variety Gazette - Volume 2 Issue 1
Traits
23.
24.
(*)
25.
(*)
26.
27.
(*)
28.
29.
Characteristics
Fruit: depth of
indentation of apex
Fruit: curvature (only
for cylindrical types)
Fruit: color of skin at
commercial
harvesting
Fruit: intensity of
color of skin (except
for white; as for 25)
Fruit: stripes
Fruit: density of
stripes
Fruit: glossiness at
harvest maturity
Test Guidelines
shallow
Note/
Remarks
3
medium
5
deep
7
absent or very weak
1
weak
3
medium
5
strong
7
very strong
9
white
1
yellow
2
green
3
mauve
4
purple
5
light
3
medium
5
dark
7
absent
1
present
9
sparse
3
medium
5
dense
7
weak
3
medium
5
strong
7
Description/Category
98
Reference
(Example)Varieties
Plant Variety Gazette - Volume 2 Issue 1
Traits
30.
(*)
31.
32.
33.
(*)
34.
35.
(*)
36.
37.
Characteristics
Fruit: ribs
Fruit: prominence of
ribs
Fruit: size of calyx
Fruit: anthocyanin
coloration lower
portion of calyx
Fruit: intensity of
anthocyanin
coloration lower
portion of calyx
Fruit: anthocyanin
coloration of calyx
Fruit: intensity of
anthocyanin
coloration of calyx
Fruit: spininess of
calyx
Test Guidelines
absent
Note/
Remarks
1
present
9
weak
3
medium
5
strong
7
small
3
medium
5
large
7
absent
1
present
9
weak
3
medium
5
strong
7
absent
1
present
9
very weak
1
weak
3
medium
5
strong
7
very strong
9
absent or very weak
1
weak
3
medium
5
strong
7
very strong
9
Description/Category
99
Reference
(Example)Varieties
Test Guidelines
Plant Variety Gazette - Volume 2 Issue 1
Traits
38.
(*)
39.
40.
(*)
41.
(*)
42.
43.
44.
45.
Characteristics
Fruit: color of flesh
Fruit: color of skin at
physiological
ripeness
Time of beginning of
flowering
Time of physiological
ripeness
Fruit firmness
Fruit shelf life (under
ambient condition;
average no of days
from harvest to 50%
of the fruits still
marketable)
Resistance to Leaf
hopper
(Rating scale: 1-least
damage to 5-most
severe damage; data
taken from field
screening)
Resistance to
Eggplant shoot and
fruit borer (ESFB)
(Rating of EFSB
based on fruit
damaged; data taken
from field screening)
whitish
Note/
Remarks
1
greenish
2
yellow
1
ochre
2
brown
3
early
3
medium
5
late
7
early
3
medium
5
late
7
soft
3
medium
5
firm
7
short
3
medium
5
long
7
susceptible
3
intermediate
5
resistant
7
susceptible
3
intermediate
5
resistant
7
Description/Category
100
Reference
(Example)Varieties
Plant Variety Gazette - Volume 2 Issue 1
Traits
46.
47.
Characteristics
Resistance to
Bacterial Wilt
(based on seedling
screening)
Resistance to
Phomopsis
Test Guidelines
susceptible
Note/
Remarks
3
intermediate
5
resistant
7
susceptible
3
intermediate
5
resistant
7
Description/Category
101
Reference
(Example)Varieties
Plant Variety Gazette - Volume 2 Issue 1
VIII.
Test Guidelines
Explanations on the Table of Characteristics
Leaf : margin
1
entire
2
dentate
3
sinuate
Fruit : general shape
1
pear-shaped
2
ovoid
3
globular
4
cylindrical
Fruit: shape of apex
1
indented
2
rounded
102
3
pointed
Plant Variety Gazette - Volume 2 Issue 1
IX.
Test Guidelines
Literature
 Khan, R., 1979: “Solanum melongena and the problems of its origin and
phylogenetic affinities,” J. Indian Bot. Soc., 58; pp. 99-109
 Khan, R., 1979: “Solanum melongena and its ancestral forms,” In: Hawkes,
J.G., Lester, R.N. and Skelding, A.D. eds.: “The biology and taxonomy of the
Solanaceae,” London, Academic Press, pp. 629-636
 Martin, F.W. and Rhodes, A.M., 1979: “Subspecific grouping of egg plant
cultivars,“ Euphytica 28, pp. 367-383
 Pearce, K., and Lester, R.N., 1979: “Chemotaxonomy of the cultivated egg
plant – a new look at the taxonomic relationships of Solanum melongena.” In:
Hawkes, J.G., Lester, R.N., and Skelding, A.D., eds.: “The biology and taxonomy
of the Solanaceae.” London, Academic Press, pp. 616-628
 Tindall, H.D., 1983: “Local Garden Egg.” In: “Vegetables in the Tropics.”
London, MacMillan Press, pp. 360-365
103
Test Guidelines
Plant Variety Gazette - Volume 2 Issue 1
X.
Technical Questionnaire
Reference Number:
(not to be filled in by the applicant)
TECHNICAL QUESTIONNAIRE
to be completed in connection with an application for plant breeder‟s rights
1. Species : Solanum melongena L. EGG PLANT
2. Applicant (Name and address)
3. Proposed denomination or breeder‟s reference
4. Information on origin, maintenance and reproduction of the variety
5. Characteristics of the variety to be given (the number in brackets refers to the corresponding
characteristics in the Rest Guidelines; please mark the state of expression which best
corresponds)
Characteristics
5.1
(17)
5.2
(19)
Fruit: length
Fruit: ratio length/
diameter
Description/Category
short
Reference
(Example)Varieties
Note/
Remarks
3
medium
5
long
7
Very small
1
Small
3
Medium
5
Large
7
Very large
9
104
Plant Variety Gazette - Volume 2 Issue 1
Characteristics
5.3
(20)
5.4
(21)
5.5
(22)
5.6
(24)
5.7
(25)
5.8
(27)
5.9
(35)
Fruit: general shape
Fruit: size of pistil
scar
Fruit: shape of apex
Fruit: curvature (only
for cylindrical types)
Fruit: color of skin at
commercial
harvesting
Fruit: stripes
Fruit: anthocyanin
coloration of calyx
Test Guidelines
Description/Category
Pear-shaped
Reference
(Example)Varieties
Note/
Remarks
1
Ovoid
2
Globular
3
Cylindrical
4
Small
3
Medium
5
Large
7
Indented
1
Rounded
2
Pointed
3
Absent or very weak
1[ ]
Weak
3[ ]
Medium
5[ ]
Strong
7[ ]
Very strong
9[ ]
White
1[ ]
Yellow
2[ ]
Green
3[ ]
Mauve
4[ ]
Purple
5[ ]
Absent
1[ ]
Present
9[ ]
Absent
1[ ]
Present
9[ ]
105
Plant Variety Gazette - Volume 2 Issue 1
Characteristics
5.10
(38)
Fruit: color of flesh
Test Guidelines
Whitish
Note/
Remarks
1[ ]
Greenish
2[ ]
Description/Category
Reference
(Example)Varieties
6. Similar varieties and differences from these varieties
Denomination of varieties
Differences
7. Additional information which may help to distinguish the variety
7.1 Resistance to pests and diseases
7.2 Special conditions for the examination of the variety
7.3 Other information
end of document
106
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Watermelon (Citrullus lanatus (Thunb.) Matsum. et Nakai.)
I.
Subject of these Guidelines
These Test Guidelines apply to all varieties of Citrullus lanatus, (Thunb.)
Matsum. et Nakai.
II.
III.
IV.
Material Required

The Plant Variety Protection Office (PVPO) decides, when, where and in what
quantity and quality the seed required for testing the variety is to be
delivered. Applicants outside the Philippines must make sure that all custom
formalities are complied with. As a minimum, for each year of test the
following quantity of seed is recommended. 400 seeds

The plant material must not have undergone any treatment unless the
competent authorities allow or request such treatment. It has been treated,
full details of the treatment must be given.
Conduct of Tests

The minimum duration of tests should be two similar growing periods.

The tests should be normally conducted in one place. If any important
characteristics of the variety can not be seen at that place, the variety maybe
tested at an additional place.

The tests should be carried out under conditions ensuring normal growth.
The size of the plots should be such that plants or plant parts of plants maybe
removed for measurement and counting without prejudice to observations
which must be made up to the end of the growing period. As a minimum,
each test should include 35 plants. In all cases the total number of plants
should be divided between two or more replicates. Separate plots for
observation and for measuring can only be used if they have been subject to
similar environmental condition.

Additional tests for special purposes may be established.
Methods and Observations

All plants indicated under Chapter III above should be used for the testing of
uniformity. A population standard of 1% and an acceptance probability of
95% should be applied. The maximum number of types allowed would be
one.

Unless otherwise indicated, all observations determined by measurement
should b e made on 20 plants or parts of 20 plants.
107
Plant Variety Gazette - Volume 2 Issue 1
V.
Test Guidelines

All observations on the leaf should be recorded on fully developed leaves
during the appearance of the first female flower.

Unless otherwise indicated, all observations on the fruit should be made on
first well developed, mature fruits.

All observations on the seeds should be made on mature and dry seeds after
washing and drying in the shade.

When resistance characteristics are used for assessing distinctness.
homogeneity and stability, records must be taken under conditions of
controlled infection.
Grouping of Varieties
The collection to be grown should be divided into groups to facilitate he
assessment of distinctness. Characteristics which are suitable for grouping purposes
are those which are known from experience not to vary, or to vary only slightly,
within a variety and which in their various states are fairly distributed within the
collection.
It is recommended that the competent authorities use the following
characteristics for grouping varieties:



VI.
Ploidy
Fruit
Fruit
: (Characteristic 1)
: shape of longitudinal section (characteristic 29)
: ground color of skin (characteristic 30)
Characteristics and Symbols

To assess distinctness, homogeneity and stability, the characteristics in the
Table of characteristics should be used.

Notes (1-9), for the purposes of electronic data processing, are given opposite
the states of the different characteristics

Legend
(*) Characteristics that should be used every growing period for the
examinations of all the varieties and should always be included in the
description of the variety, except when the state of expression of a preceding
characteristic or regional environmental conditions render this impossible.
(+) See explanations on the Table of Characteristics in Chapter VIII.
108
Plant Variety Gazette - Volume 2 Issue 1
VII.
Test Guidelines
Table of Characteristics
Traits
1
(*)
(*) 2
(*) 3
Characteristics
Ploidy
Seed: size
Seed: ground color
of testa
diploid
Note/
Remarks
2
triploid
3
tetraploid
4
very small
1
small
3
medium
5
large
7
very large
9
white
1
cream
2
green
3
red
4
Description/Category
Reference
Varieties
light brown
4
5
Seed: secondary
color of testa
Seed: type of
distribution of
secondary color of
testa
red brown
5
brown
6
black
7
absent
1
present
9
in dots only
1
in patches only
2
in dots and in patches
3
109
Plant Variety Gazette - Volume 2 Issue 1
Traits
6
7
8
9
(*)
10
11
12
(*)
13
Characteristics
Seed: area of
secondary color in
relation to that of
ground color
Seed: patches at
hilum
Seed: patches at
margin
Seedling: shape of
Cotyledon
Seedling: size of
cotyledon
Seedling: Intensity
of green color of
cotyledon
Seedling: Spots on
Cotyledon
Seedling:
Depression of
nerves of
Cotyledon
Test Guidelines
small
Note/
Remarks
3
medium
5
large
7
absent
1
present
9
absent
1
present
9
narrow elliptic
1
elliptic
2
broad elliptic
3
small
3
medium
5
large
7
light
3
medium
5
dark
7
absent
1
present
9
absent
1
present
9
Description/Category
110
Reference
Varieties
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Traits
Characteristics
Description/Category
14
Seedling: Length of
hypocotyls
short
Note/
Remarks
3
medium
5
long
7
bush
1
runner
2
short
3
medium
5
long
7
absent
1
present
9
low
3
medium
5
high
7
early
3
medium
5
late
7
early
3
medium
5
late
7
short
3
medium
5
long
7
15
16
17
(*)
18
19
(*)
20
21
(*)
Plant: growth habit
Plant: Length of
main stem (at the
time of harvest of
first fruit)
Plant:
Hermaphrodite
flowers
Plant: Number of
nodes up to the
first node with
female flowers
Time of female
flowering (50% of
plants with at least
one female flower
from sowing)
Time of maturity
(50% of plants with
at least one ripe
fruit from sowing)
Leaf Blade: Length
111
Reference
Varieties
Plant Variety Gazette - Volume 2 Issue 1
Traits
22
(*)
23
24
25
26
27
(*)
(+)
28
29
Characteristics
Leaf Blade: width
Leaf Blade: Ratio
length/width
Leaf /blade: color
Leaf/blade:
intensity of color
Leaf: Degree of
lobing (beyond first
flower)
Leaf: depth of
incisions of margin
of leaf of central
third of plant (as of
17)
Leaf Blade:
blistering
Leaf Blade:
undulation of
margin
Test Guidelines
narrow
Note/
Remarks
3
medium
5
broad
7
small
3
medium
5
large
7
yellow green
1
green
2
gray green
3
light
3
medium
5
dark
7
weak
3
medium
5
strong
7
shallow
3
medium
5
deep
7
weak
3
medium
5
strong
7
weak
3
medium
5
strong
7
Description/Category
112
Reference
Varieties
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Traits
Characteristics
Description/Category
30
(*)
Leaf Blade: flecking
absent
Note/
Remarks
1
present
9
short
3
medium
5
long
7
small
3
medium
5
large
7
acute
3
rounded
5
obtuse
7
weak
3
medium
5
strong
7
small
3
medium
5
large
7
Weak
3
medium
5
strong
7
31
32
33
34
35
36
Petiole: length
Flower: size of
petal of female
flower (third to
seventh flower)
Flower: Shape of
apex of petal of
female flower
Flower: anther
dehiscence at low
temperature
Ovary: size
Ovary: Pubescence
113
Reference
Varieties
Plant Variety Gazette - Volume 2 Issue 1
Traits
37
(*) 38
(+)
(*) 39
(+)
(*) 40
Characteristics
Fruit: weight (kg)
Fruit: shape of
longitudinal section
Fruit: ground color
of skin
Fruit: intensity of
green color of skin
Test Guidelines
very low
Note/
Remarks
1
very low to low
2
low
3
low to medium
4
medium
5
medium to high
6
high
7
high to very high
8
high
9
round
1
broad elliptic
2
elliptic
3
cylindric
4
white
1
yellow
2
green
3
very light
1
very light to light
2
light
3
light to medium
4
medium
5
medium to dark
6
dark
7
dark to very dark
8
very dark
9
Description/Category
114
Reference
Varieties
Plant Variety Gazette - Volume 2 Issue 1
Traits
41
42
(+)
(*) 43
(+)
44
(*) 45
(+)
46
Characteristics
Fruit: length of
peduncle
Fruit: size of
insertion of
peduncle
Fruit: shape of
basal part
Fruit: depression of
base
Fruit: shape of
apical part
Fruit: depression at
apex
Test Guidelines
short
Note/
Remarks
3
medium
5
long
7
small
3
medium
5
large
7
flat
1
flat to rounded
2
rounded
3
rounded to conical
4
conical
5
shallow
3
medium
5
deep
7
Flat
1
flat to rounded
2
rounded
3
rounded to conical
4
conical
5
shallow
3
medium
5
deep
7
Description/Category
115
Reference
Varieties
Plant Variety Gazette - Volume 2 Issue 1
Traits
47
(*) 48
(*) 49
(+)
(*) 50
(+
51
52
Characteristics
Fruit: size of pistil
scar
Fruit: grooves
Fruit: stripes
Fruit: intensity of
green color of
stripes
Fruit: width of
stripes
Fruit: marbling
Test Guidelines
small
Note/
Remarks
3
medium
5
Large
7
Absent
1
at basal half
2
at apical half
3
on whole fruit
4
absent
1
present
9
very light
1
light
3
medium
5
dark
7
very dark
9
very narrow
1
narrow
3
medium
5
broad
7
very broad
9
absent
1
present
9
Description/Category
116
Reference
Varieties
Plant Variety Gazette - Volume 2 Issue 1
Traits
53
(*) 54
(+)
(*) 55
56
57
Characteristics
Fruit: intensity of
marbling
Fruit: thickness of
outer layer of
pericarp
Fruit: main color of
flesh
Fruit: intensity of
main color of flesh
Fruit: firmness of
flesh
Test Guidelines
very weak
Note/
Remarks
1
weak
3
medium
5
strong
7
very strong
9
thin
3
medium
5
thick
7
white
1
yellow
2
orange
3
red
4
purple
5
others (pinkish red)
7
light
3
medium
5
dark
7
soft
3
medium
5
firm
7
Description/Category
117
Reference
Varieties
Test Guidelines
Plant Variety Gazette - Volume 2 Issue 1
Traits
58
59(+)
60
61
62
63
64
65
Characteristics
Fruit: number of
seeds
Resistance to
Fusarium oxysporum
f.sp. niveum (E.F.
Smith) Snyder et
Hansen
Resistance to
Colletotrichum
lagenarium
(passerini) Ellis et
Halsted
Resistance to
Downy mildew
Resistance to
Powdery mildew
Resistance to
Watermelon mosaic
Resistance to
aphids
Resistance to fruit
worm
absent or very few
Note/
Remarks
1
few
3
medium
5
many
7
very many
9
absent
1
present
9
absent
1
present
9
absent
1
present
9
absent
1
present
9
absent
1
present
9
absent
1
present
9
absent
1
present
9
Description/Category
118
Reference
Varieties
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Traits
Characteristics
Description/Category
66
Resistance to thrips
absent
Note/
Remarks
1
present
9
absent
1
present
9
67
VIII.
Resistance to
whitefly
Reference
Varieties
Explanation on the Table of Characteristics
Trait No. 2 Seedlings : shape of cotyledon
narrow elliptic
elliptic
broad elliptic
Trait No. 18 Leaf blade : depth of incisions of margin of leaf of central third of plant
The incisions should be observed at the largest leaf between the fifteenth and
twentieth node of the main stem.
shallow
medium
119
deep
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Trait No. 29 Fruit : Shape of longitudinal section
1
round
2
broad elliptic
3
elliptic
4
cylindric
Traits No. 30, 40, 41 Fruit : ground color of skin and color of stripes
The ground color is defined as the lighter color and the color of the stripes as the
darker color.
Traits No. 33, 34, 36, 45
Fruit
basal part (34)
thickness of out layer of pericarp
apical part
insertion of peduncle
120
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Trait No. 59 Resistance to Fusarium oxysporum f. sp. niveum (E. F. Smith)
Snyder et Hansen
Method
Maintenance of races
Type of medium:
P.S.A. (Potatio, Sugar and Agar) medium
Special conditions:
Stored below 5oC
Preparation of inoculum:
Shaking culture in P. S. (Potato and Sugar) liquid
medium for 7 to 10 days at 28oC. Filtration by using
double gauzes. Adjusting concentration of spore to 1.5
x 107/ml with sterilized water.
Execution of test
Sowing the seeds:
In sterilized soil
Growth stage of plants:
Expanding of first true leaf
Method of inoculation:
Soaking of roots and of hypocotyls axis for one minute
in inoculum solution. After inoculation, transportation
of plantlets in sterilized (by steam) soil or perlite.
Number of plants tested:
10 to 20 plants
Environmental condition after inoculation
Temperature:
Day: 25oC; night: 16oC
Light:
Natural (longer than 12 hours)
Growing method:
In the glasshouse or climatic room. Application of
liquid fertilizer every week.
Duration of test
Inoculation – last observation:
Remarks
Keeping of pathogenecity:
20 days. Disease symptoms appear from 5 to 10 days
after inoculation. Observation should be made on
several occasions.
Renewal of medium at least once a year.
Standard varieties:
Black Diamond, Kaho
Charleston Gray
Calhoun Gray
P.I. 296341-FR
Race 0
S
R
R
R
Race 1
S
S
R
R
S: susceptible
121
Race 2
S
S
R
R
R: resistant
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Trait No. 60 Resistance to Colletotrichum lagenarium (passerini) Ellis et Halsted
Maintenance of races
Type of medium:
P.S.A. (Potatio, Sugar and Agar) medium
Special conditions:
Stored below 5oC
Preparation of inoculum:
Shaking culture in P. S. (Potato and Sugar) liquid
medium for 7 to 10 days at 28oC. Filtration by using
double gauzes. Adjusting concentration of spore to 1.5
x 107/ml with sterilized water.
Execution of test
Sowing the seeds:
In sterilized soil
Growth stage of plants:
Expanding of 2nd to 3rd true leaf
Method of inoculation:
Inoculated plants should be placed in a dark and humid
chamber at 25oC with 100% relative humidity for 48
hours before being moved to glasshouse.
Number of plants tested:
10 to 20 plants
Environmental condition after inoculation
Temperature:
Day: 25oC; night: 16oC
Light:
Natural (longer than 12 hours)
Growing method:
In the glasshouse
Duration of test
Inoculation – last observation:
25 days
Remarks
Race:
Three races are identified
Keeping of pathogenecity:
Renewal of medium at least once a year.
Standard varieties:
Kaho
Charleston Gray
Congo
African citron W-695
Race 0
Race 1
S
R
S
S
Race
2
S
R
S
R
S
S: susceptible
122
R: resistant
Test Guidelines
Plant Variety Gazette - Volume 2 Issue 1
IX.
Literature
 CRALL, J.M., 1959: “Effect of Seed source on Watermelon Maturity,”
Proc.Amer.Soc.Hort.Sci. 74, pp 555-557
 CRALL, J.M., MONTELARO, J., 1972: “Fusarium Wilt Resistance in Jubilee
Watermelon,” Proc.Fra. State Hoest. Soc. 85, pp 102-105
 Cucurbit Genetics Cooperative, Cucurbit Gene List Committee, 1987: “Gene List
for Watermelon,” Cucurbit Gent. Coop. Rpt. 10, pp 106-110
 ELMSTROM, G.W., HOPKINS, D.L., 1981: “Resistance of Watermelon Cultivars
to Fusarium Wilt” Plant Disease 65(10), pp 825-827
 KANDA, T., 1951: “Triploid Watermelons,” Proc.Am.Soc.Hortic.Sci.58, pp 217230
 KENSLER, T.R., BARHAM, W.S., 1958: “The Inheritance of Seed Size in
Watermelon,” Proc.Amer.Soc. Hort.Sci. 71, pp 480-484
 MARTYN, R.D., MCLAUGHLYN, R.J., 1983: “Susceptibility of Summer Squash
to the Watermelon Wilt Pathogen (Fusarium osyxporum f. sp. niveum),” Plant
Disease 67(3), pp 263-266
 MARTYN, R.D., NETZER, D., 1991: “Resistance to Race 0, 1 and 2 of Fusarium
Wilt of Watermelon in Citrullus sp.,” PI-296341-FR
 MIZUNO, S., PRATT, H.K., 1973: “Relations of Respiration and Ethylene
Production to Matury in the Watermelon,” J.Amer.Soc.Hort.Sci. 98(6), pp 614-617
 MOHR, H.C., 1963: “Utilization of the Genetic Character for Short-internode in
Improvement of the Watermelon”, J.Amer.Soc.Hort.Sci. 82, pp 454-459
 POOL, C.F., PORTER, D.R., 1933: “pollen Gemination and Development in
Watermelon,” Proc.Amer.Soc.Hort.Sci. 30, pp 526-530
 POOL, C.F., GRIMBALL, P.C., PORTER, D.R., 1941: “Inheritance of Seed Characters
in Watermelon,” Jour.Agr.Res. 66, pp 433-456
 SHIMOTSUMA, M., JONES, C.M., 1972: “Effect of Ethephon and Daylight on Sex
Expression of Muskmelon and Watermelon,” Hort.Sci. 7, pp 73-75
123
Plant Variety Gazette - Volume 2 Issue 1
X.
Test Guidelines
Technical Questionnaire
Reference Number:
(not to be filled in by the applicant)
TECHNICAL QUESTIONAIRE
to be completed in connection with an application for plant breeders‟ rights
1. Species : Citrullus lanatus (Thumb.) Matsum. Et Nakai WATERMELON
2. Applicant (Name and address)
3. Proposed denomination or breeder‟s reference
4. Information on origin, maintenance and reproduction of the variety
4.1 Method of maintenance and reproduction
 Hybrid
[ ]
 Open pollinated variety
[ ]
4.2 Other Information
5. Characteristics of the variety to be given (the number in brackets refers to the
corresponding characteristic in the Test Guidelines; please mark the state of expression
which best corresponds.
Characteristics
5.1
(1)
Ploidy
Description/Category
Example Varieties
Note
diploid
2[ ]
triploid
3[ ]
tetraploid
4[ ]
124
Plant Variety Gazette - Volume 2 Issue 1
Characteristics
5.2
(37)
5.3
(38)
5.4
(39)
55.
(49)
5.6
(55)
Fruit: weight
Fruit: shape of
longitudinal section
Fruit: ground color of
skin
Fruit: stripes
Fruit: main color of
flesh
Test Guidelines
Description/Category
Example Varieties
Note
very low
1[ ]
very low to low
2[ ]
low
3[ ]
low to medium
4[ ]
medium
5[ ]
medium to high
6[ ]
high
7[ ]
high to very high
8[ ]
very high
9[ ]
round
1[ ]
broad elliptic
2[ ]
elliptic
3[ ]
cylindric
4[ ]
white
1[ ]
yellow
2[ ]
green
absent
3[ ]
1[ ]
present
white
9[ ]
1[ ]
yellow
2[ ]
orange
3[ ]
red
4[ ]
purple
5[ ]
125
Test Guidelines
Plant Variety Gazette - Volume 2 Issue 1
6. Similar varieties and differences from these varieties
Denomination of
similar variety
Characteristic in
which the similar
variety is different1
State of expression of
similar variety
State of
expression of
candidate variety
In the case of identical states of expression of both varieties, please indicate the size of the
difference.
1
7.
Additional information which may help to distinguish the variety.
7.1 Resistance to pests and diseases
(i)
Fusarium oxysporum f. niveum (E.F.
Smith) Snyder et Hansen
Race 0
Race 1
Race 3
Absent
Present
Not Tested
[ ]
[ ]
[ ]
[ ]
[ ]
[ ]
[ ]
[ ]
[ ]
[ ]
[ ]
[ ]
[ ]
[ ]
[ ]
(ii)
Colletotrichum lagenarium (passerini)
Ellis et Halsted
[ ]
Race 0
[ ]
Race 1
[ ]
Race 3
(iii) Other resistance (specify)
Downy mildew
Powdery mildew
Aphids
Thrips
White flies
Fruit Worm
7.2 Special conditions for the examination of the variety
(i) Use
- in the greenhouse
[]
- in the open
[]
- in the open and in greenhouse
[]
(ii) Other conditions
7.3 Other information
end of document
126
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Onion and Shallot (Allium cepa L, Allium cepa var. ascalonicum L.)
I.
Subject of these Guidelines
These Test Guidelines apply to all seed and vegetatively propagated varieties of
onion (Allium cepa L.) and multiplier onion or shallot (A. cepa var. ascalonicum)
II.
Material Required

The Plant Variety Protection Office (PVPO) decides where and in what
quantity and quality the plant material required for testing the variety is to be
delivered. Applicants submitting material outside the Philippines must make
sure all customs formalities are complied with. The minimum quantity of
plant material or seed to be supplied by the applicant in one or several
samples should be:


III.
IV.
Seed propagated varieties : 30g of seed
Vegetatively propagated varieties : 500 bulblets or sets

The seeds submitted for testing must have at least 90% germination, utmost
10% moisture content and 99% purity.

The seed and plant material must not have undergone any treatment unless
the PVPO requires such treatment. If it has been treated, full details of the
treatment must be given.
Conduct of Tests

A minimum of two tests will be required and these be conducted during the
dry season at one place. If any important characteristics of the variety cannot
be seen at that place, the variety may be tested at an additional location.

The tests should be carried out under conditions ensuring normal growth.
The size of the plots should be 1 x 5 m. or 10 sq. m. for the two replications
following 20 cm x 20 cm planting distance. Each test should include a total of
250 plants. Separate plots for observation and for measuring can only be used
if they have been subject to similar environmental conditions.

Additional tests for special purposes may be established.
Methods and Observations
All observations determined by measurement or counting should be made on
60 plants or parts taken from each of 60 plants.
For the assessment of uniformity of vegetatively propagated varieties, a
population standard of 1% with an acceptance probability of at least 95% should be
127
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
applied. In the case of a sample size of 100 plants the maximum number of off-types
allowed would be 3.
For the assessment of uniformity of open-pollinated and hybrid varieties
relative uniformity standards should be applied.


V.
All observations on the leaf and the foliage should be made on the fifth
leaf stage.
All observations on the bulb should be made at harvest maturity.
Grouping of Varieties
The collection of varieties to be grown should be divided into groups to
facilitate the assessment of distinctness. Characteristics which are suitable for growing
purposes are those which are know from experience not to vary, or to vary only
slightly, within a variety. Their various stage of expression should be fairly
distributed throughout the collection.
It is recommended that the following characteristics will be used for grouping
varieties:



VI.
Bulb: splitting into bulblets (with dry skin around each bulblet)
(characteristic 10)
Bulb/Bulblet: general shape (in longitudinal section) (characteristic 18)
Bulb/Bulblet: basic color for dry skin ( characteristic 23)
Characteristics and Symbols
To assess distinctness, uniformity and stability, the characteristics and their
states as given in the Table of Characteristics should be used. The name of each
example variety is followed by an abbreviation indicating whether the variety is an
onion (O) or a shallot (S).
Notes (numbers), for purposes of electronic data processing, are given
opposite the states of expression for each characteristic.
Legend:
(*)
Characteristics that should be used on all varieties in every growing period
over which the examinations are made and always be included in the variety
descriptions, except when the state of expression of a preceding characteristics or
regional environmental conditions render this impossible.
(+)
See Explanations on the Table of Characteristics in Chapter VIII.
(1)
The optimum stage of development for the assessment of each characteristic is
indicated by a number in the second column. The stages of development denoted by
each number are described at the end of the Chapter VIII.
(2)
Type of example variety: O = Onion, S = Shallot.
128
Plant Variety Gazette - Volume 2 Issue 1
VII.
Table of Characteristics
Trait
No.
Characteristics
1.
(*)
Plant: number of leaves
per pseudostem
2
(*)
3
(*)
4.
(*)
5.
(* )
(+)
6.1
Test Guidelines
Foliage: orientation
Foliage: waxiness
Foliage: green color
Foliage: cranking
Onion varieties only:
Leaf: length
Description /
Category
Reference (Example)Varities
Onion
Shallot
Note /
Remarks
few
3
medium
5
many
7
erect
1
semi-erect
2
horizontal
3
absent
1
medium
5
strong
9
light
3
medium
5
dark
7
absent or very
weak
1
weak
3
medium
5
strong
7
very strong
9
very short
1
short
3
medium
5
long
7
very long
9
129
Test Guidelines
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
6.2
7.1
(*)
7.2
(*)
8.
(+)
9.
(+)
Characteristics
Shallot varieties only:
Leaf: length
Onion varieties only:
Leaf: diameter
Shallot varieties only:
Leaf: diameter
Onion varieties only:
Pseudostem: length (up
to highest green leaf)
Onion varieties only:
Pseudostem: diameter
(at midpoint of length)
Description /
Category
Reference (Example)Varities
Onion
Shallot
Note /
Remarks
very short
1
short
3
medium
5
long
7
very long
9
small
3
medium
5
large
7
small
3
medium
5
large
7
short
3
medium
5
long
7
small
3
medium
5
large
7
10.a
(*)
(+)
Bulb:splitting into
bulblet (with dry skin
around each bulblet)
absent
1
present
9
10.b
(*)
Bulb: internal split
absent
1
present
9
weak (1-2)
3
medium (3-4)
5
strong ( more
than 5)
7
11
(*)
Shallot varieties only:
Bulb: degree of
splitting into bulblet
130
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
12.a
(*)
12.b
(*)
13.a
(*)
13.b
(*)
14.a
(*)
14.b
(*)
15.a
(*)
Characteristics
Onion varieties only:
Bulb: size
Shallot varieties grown
from bulblets only:
Bulblet: size
Onion varieties only:
bulb Polar diameter
Shallot varieties grown
from bulblets only:
Bulblet: polar diameter
Onion varieties only:
Bulb: equatorial
diameter
Shallot varieties grown
from bulblet only:
Bulblet: equatorial
diameter
Onion varieties only:
Bulb:ratio
height/diameter
Test Guidelines
Description /
Category
Reference (Example)Varities
Onion
Shallot
Note /
Remarks
small
3
medium
5
large
7
small
3
medium
5
large
7
short
3
medium
5
tall
7
short
3
medium
5
tall
7
small
3
medium
5
large
7
small
3
medium
5
large
7
small
3
medium
5
large
7
131
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
15.b
(*)
16
(*)
(+)
17
(+)
18
(*)
(+)
19.
(*)
(+)
Characteristics
Shallot varieties from
Bulblet only: Bulblet:
Ratio height/diameter
Bulb/Bulblet: position
of maximum diameter
Bulb/Bulblet: width of
neck
Bulb/Bulblet: general
shape (in longitudinal
section)
Onion varieties only:
Bulb: shape of top (as
for 18) Depressed
Test Guidelines
Description /
Category
Reference (Example)Varities
Onion
Shallot
Note /
Remarks
small
3
medium
5
large
7
towards apex
1
at middle
2
towards base
3
narrow
3
medium
5
broad
7
elliptic
1
ovate
2
broad elliptic
3
circular
4
broad ovate
5
broad obovate
6
rhombic
7
transverse
elliptic
8
transverse
narrow elliptic
9
flat
1
slightly raised
2
rounded
3
slightly sloping
4
strongly
sloping
5
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Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
20.
(*)
(+)
21.
22.
23.
(*)
24.
(*)
Characteristics
Bulb/Bulblet: shape of
base (as for 18)
Bulb/Bulblet:
adherence of dry skin
after harvest
Bulb/Bulblet: thickness
of dry skin
Bulb/Bulblet: basic
color of dry skin
Bulb/Bulblet: intensity
of basic color of dry
skin
Test Guidelines
Description /
Category
Reference (Example)Varities
Onion
Shallot
Note /
Remarks
recessed
1
flat
2
round
3
weakly tapered
4
strongly
tapered
5
weak
3
medium
5
strong
7
thin
3
medium
5
thick
7
white
1
gray
2
green
3
yellow
4
brown
5
pink
6
red
7
light
3
medium
5
dark
7
133
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
25.
(*)
26.
(*)
27.
(*)
(+)
28.
(+)
29.
Characteristics
Bulb/Bulblet: hue of
color of dry skin (in
addition to basic color)
Bulb/Bulblet:
coloration of epidermis
of fleshy scales
Bulb/Bulblet: number
of axes
Bulb/Bulblet: dry
matter content
Onion: tendency to bolt
Test Guidelines
Description /
Category
Reference (Example)Varities
Onion
Shallot
Note /
Remarks
absent
1
grayish
2
greenish
3
yellowish
4
brownish
5
pinkish
6
reddish
7
purplish
8
absent
1
greenish
2
reddish
3
very few
1
few
3
medium
5
many
7
very low
1
low
3
medium
5
high
7
very high
9
absent or very
weak
weak
1
medium
5
strong
7
very strong
9
134
3
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
30.
31.a
31.b
32.
(*)
33.
33.1
33.2
Characteristics
Onion varieties only:
time of beginning of
bolting
Onion varieties only:
time of harvest
maturity
Shallot varieties only:
time of harvest
maturity
Time of sprouting
during storage
Reaction to Pest
Purple blotch
Cercospora leaf spot
Test Guidelines
Description /
Category
Reference (Example)Varities
Onion
Shallot
early
3
medium
5
late
7
early
3
medium
5
late
7
early
Australia,
Farmer‟s
Batanes
3
medium
Tanduyong
5
late
Batanes
7
early
Batanes
3
medium
Tanduyong
5
7
late
Red Pinoy,
Nueventa
Farmer‟s
Batanes
susceptible
Red Pinoy,
Nueventa
Farmer‟s
Batanes
resistant
6 S (Sumenep)
susceptible
Red Pinoy,
Nueventa
Farmer‟s
Batanes
Red Pinoy,
Nueventa
Farmer‟s
Batanes
Red Pinoy,
Nueventa
Farmer‟s
Batanes
Red Pinoy,
Nueventa
Farmer‟s
Batanes
resistant
33.3
Bulb rot
susceptible
resistant
33.4
Anthracnose
susceptible
resistant
33.5
Nematode
Note /
Remarks
susceptible
resistant
135
Plant Variety Gazette - Volume 2 Issue 1
VIII.
Test Guidelines
Explanations on the Table of Characteristics
Trait No. 5
Foliage : cranking
1
absent
or
very weak
3
weak
5
medium
7
strong
9
very strong
Trait No. 8 and 9
Onion varieties only : pseudostem : length (up to highest green leaf) (8) and
diameter (at midpoint of length) (9)
Trait No. 10
Bulb : splitting into bulblets (with dry skin around each bulblet)
1
absent
9
present
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Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Trait No. 16 Bulb/Bulblet : position of maximum diameter
1
towards apex
2
at middle
3
towards base
Trait No. 17 Bulb/Bulblet : width of neck
1
very narrow
3
narrow
5
medium
7
broad
9
very broad
Trait No. 18 Bulb/Bulblet : general shape ( in longitudinal section)
1
elliptic
2
ovate
3
broad
elliptic
137
4
circular
5
broad
ovate
Plant Variety Gazette - Volume 2 Issue 1
6
broad
obovate
Test Guidelines
7
rhombic
9
tranverse
narrow elliptic
8
tranverse
elliptic
Ad. 19 Onion varieties only : Bulb : shape of top (as for 18)
1
depressed
2
flat
3
slightly
raised
4
rounded
5
slightly
sloping
6
strongly
sloping
Ad. 20 Bulb/Bulblet : shape of base (as for 18)
1
recessed
flat
2
flat
3
round
138
4
weakly
tapered
5
strongly
tapered
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Ad. 27 Bulb/Bulblet: number of axes
The number of axes should be assessed when the bulb/bulblet has completely died
back and before there is any sprouting. The bulb or bulblet should be cut in transverse
section, starting at the widest point and make further cuts towards the base of the
bulb/bulblet, until there is consistency in the number of axes. Each axis appears as a point,
often greenish in color surrounded by tissue rings.
Ad. 28 Bulb/Bulblet: dry matter content
Dry matter content should be determined according to IV-I for 3 x 20 bulbs (e.g. one
sample of 20 bulbs from each plot). From these bulbs the dry skin should be prepared by
cutting the bulbs into small pieces of 1-5 mm size. A representative sample should be
weighed directly after cutting ( we should be aware that the biodegradation of sugars and
carbohydrates starts as soon as cells are damaged). The sample should be dried for 2 hours at
1050C and then the temperature should be lowered to 650C during 22 hours. Lowering of
temperature is necessary to avoid caramelisation. The remaining weight should be assed
after 24 hours. From these figures the dry matter content may be calculated. The dry matter
content could also be assessed by obtaining the total soluble solids (TSS) using a
refractometer.
Ad. 35 Time of sprouting during storage
Care should be taken to exclude damaged bulbs. Storage temperature should be
maintained under ambient condition.
Code for the Growth Stages 1 : Seed to Bulb
Growth stage / General description
Vegetative Cycle
00
0
Dry
Germination
Seedling growth
10
15
20
25
30
35
40
Emerged seedling at „loop‟ stage
Seedling with testa above ground and still attached to cotyledon
Emergence of first true leaf
Second true leaf stage
Third leaf stage
Fourth leaf stage
Fifth leaf stage
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Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Plant Growth
Sixth leaf stage
50
55
60
65
70
75
80
85
90
95
100
105
115
120
125
130
135
140
145
150
Seventh leaf stage-first leaf senescing
Eighth leaf stage
Tenth leaf stage; second and third leaf senescing; early bulb development
Full expansion of leaves achieved; continued swelling of bulb
Beginning of foliage fall-over, weakening of false stem turgidity
Leaves dry off; bulb size continues to increase; darkening of bulb scales
Bulb at harvestable maturity
Complete die-back of foliage; bulb top seals over for dormancy
Reproductive Cycle
Start of sprouting in store – swelling of root initials or emergence of shoot at top of bulb
170.1
180.1
190.1
200.1
210.1
220.1
230.1
240.1
250.1
260.1
270.1
280.1
290.1
300.1
310.1
320.1
330.1
Sprouted bulbs with emerging leaves
Splitting of outer skin
Emergence of scape and underdeveloped spathe
Scape elongation and swelling of middle
Swelling of spathe
Splitting of spade
Enlargement of umbel
Opening of florets (where fertile)
Pollination of florets
Seed set – swelling of pollinated ovaries
Ripe seed in umbels
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Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Dry seed
150
160.2
170.2
180.2
190.2
200.2
210.2
220.2
240.2
250.2
260.2
270.2
280.2
290.2
300.2
Dry bulb with complete foliage die-back
Bulb shape becoming less rounded
Bulb shape becoming irregular with development of small splits in the dry outer skin
More than one growing point emerging from the top of the bulb
Long splits developing in the outer skin of the bulb and differentiation of bulb into bulblets
Separation ob bulblets from parent bulb except at base. Bulblets
separated from each other by a dry outer skin
Complete separation of bulblets from „parent bulb‟
Development of many leaves
Literature
 BREWSTER, J. L., 1994: “Crop Production Science in Horticulture 3: Onions and Other
Vegetables Alliums.” CAB International.
 BREWSTER, J. L. and BARNES, A., 1981: “A Comparison of Relative Growth Rates of
Different Individual Plants and Different Cultivars of Onion of Diverse Geographic
Origin at Two Temperatures and Two Light Intensities.” Journal of Applied Ecology Vol.
18, 589-604.
 BREWSTER, J. L., SALTER, P. J. and DARBY, R. J., 1977: “ Analysis of the Growth and
Yield of Over-wintered Onions.” Journal of Horticulture Science Vol. 52, 335-346.
 CLARKE, A. E., JONES, H. A. and LITTLE, T. M., 1994: “Inheritance of Bulb Colour in the
Onion.” Genetics 29, pp 569-575.
 EL-SHAFIE, M. W. and DAVIES, G. N., 1967: “ Inheritance of Bulb Color in the Onion
(Allium cepa L.).” Hilgardia Vol. 38, No. 17, 607-622.
 JONES, H. A., CLARKE, A. E. and STEVENSON, F. J., 1944: “Studies in the Genetics of
the Onion (Allium cepa L.).” Proceedings of the American Society for Horticulture Science
44, pp 479-484.
 JONES, H. A. and MANN, L. K., 1963: “onions and Their Allies: Botany, Cultivation and
Utilisation.” London, Leonard Hill.
 JONES, H. A. and PETERSON, C. E., 1952: “Complementary Factors for Light-Red Bulb
Colour in Onions.” Proceedings of the American Society for Horticulture Science Vol. 59,
457.
141
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
 KAPPERT and RUDOLF, W., 1962: “Züchtung von Gemüse, Obst, Reben and
Forstpflanzen.” Verlag Paul Parey, Berlin und Hamburg, pp 270-312.
 KUCKUCK, H. and KOBAKE, G., 1962, in ROEMER, T. and RUDOLF, W., 1962:
“Handbuch der Pfanzen-Züchtung, Band VI.” Verlag Paul Parey, Berlin und Hamburg.
 MAGRUDER, R. and ALLARD, H. A., 1937: “Bulb Formation in Some American and
European Varieties of Onions as Affected by Length of Day.” Journal of Agricultural
Research Vol. 54, Part No. 10, 719-752.
 MAGRUDER, R. et al, 1941: “Descriptions and Types of Principal American Varieties of
Onion.” USDA, Miscellaneous Publication No. 435, Washington DC.
 MESSIAEN, C. M., COHAT, L., LEROUX, J. P., PICHON, M., BEYRIES, A., 1993: “Les
allium alimentaires reproduits para voie végétative.” INRA Editions, Paris.
 MIDMORE, D. J., 1994, (Editor): “International Symposium on Alliums for the Tropics.”
Acta Horticulturae, 358.
 RABINOWITCH, H. D. and BREWSTER, J. L., 1990: “ Onions and Allied Crops.” Vol. 1-3
CRC Press, Boca Raton.
 REIEMAN, G. H., 1931: “Genetic Factors for Pigmentation in the Onion and Their
Relation to Disease Resistance.” Journal of Agricultural Research Vol. 42, No. 5, 251-278.
 SCULLY, N. J., PARKER, M. W. and BORTHWICK, H. A. 1945: “Interaction of Nitrogen
Nutrition and Photo-period as Expressed in Bulbing and Flower Stalk Development of
Onion.” Botanical Gazette Vol. 107, 52-61.
 SCWARTZ, H. F. and MOHAN, S. K., 1995 (Editors): “Compendium of Onion and Garlic
Diseases.” The American Phytopathological Society, (ISBN: 0-89-54-170-1).
 WRIGHT, C. J. and SOBEIGH, W. Y., 1986: “The Photo-periodic Regulation of Bulbing in
Onions (Allium cepa L.), Effects of Irradiance.” Journal of Horticulture Science Vol. 61, Part
3, 311-335.
142
Plant Variety Gazette - Volume 2 Issue 1
X.
Test Guidelines
Technical Questionnaire
Reference Number
(not to be filled in by the applicant)
_____________________________
_____________________________
TECHNICAL QUESTIONAIRE
to be completed in connection with an application for plant breeders‟ rights
1. Species
Allium cepa L.
Allium escalonicum L.
(a)
(b)
ONION
SHALLOT
(
(
2. Applicant (Name and address)
3. Proposed denomination or breeder‟s reference
4. Information on origin, maintenance and reproduction of the variety
4.1
Variety type
(a)
(b)
(c)
(d)
(e)
open-pollinated
single hybrid
three-way hybrid
clone
other type (indicate what type)
(
(
(
(
(
Other information
143
)
)
)
)
)
)
)
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
5. Characteristics of the variety to be indicated ( the number in brackets refers to the
corresponding characteristic in Test Guidelines; please mark the state of expression which
best corresponds).
Characteristics
5.1
(1)
5.2
(4)
5.3a
(10)
5.3b
5.4.1
(12.1)
5.4.2
(12.2)
5.5
(18)
Plant: number of leaves
per pseudostem
Foliage: green color
Bulb: splitting into
bulblets (with dry skin
around each bulblet)
Bulb: internal split
Onion varieties only:
Bulb: size
Shallow varieties grown
from blblets only:
Bulblet: size
Bulb/Bulblet: general
shape (in longitudinal
section)
Description/Category
Example
Varieties
Note
few
3[ ]
medium
5[ ]
many
7[ ]
light
3[ ]
medium
5[ ]
dark
absent
7[ ]
1[ ]
present
absent
9[ ]
1[ ]
present
small
9[ ]
3[ ]
medium
5[ ]
large
small
7[ ]
3[ ]
medium
5[ ]
large
elliptic
7[ ]
1[ ]
ovate
2[ ]
broad elliptic
3[ ]
circular
4[ ]
broad ovate
5[ ]
broad obovate
6[ ]
rhombic
7[ ]
transverse elliptic
8[ ]
transverse narrow
elliptic
9[ ]
144
Plant Variety Gazette - Volume 2 Issue 1
Characteristics
5.6
(23)
5.7
(25)
5.8
(33)
Test Guidelines
Description/Category
Bulb/Bulblet: basic color of
dry skin
Note
white
1[ ]
grey
2[ ]
green
3[ ]
yellow
4[ ]
brown
5[ ]
pink
6[ ]
red
7[ ]
absent
1[ ]
grayish
2[ ]
greenish
3[ ]
yellowish
4[ ]
brownish
5[ ]
pinkish
6[ ]
reddish
7[ ]
Bulb/bulblet: hue of color of
dry skin
Onion varieties only: Time of
harvest maturity
Example
Varieties
purplish
8[ ]
very early
1[ ]
early
2[ ]
medium
3[ ]
late
4[ ]
very late
5[ ]
145
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
6. Similar varieties and differences between these varieties
Denomination of
Characteristic in which the
State of expression
similar variety
similar variety is different o)
of similar variety
State of expression of
candidate variety
o)
In the case of identical states of expressions of both varieties, please indicate the size of the
difference.
7.
Additional information which may help to distinguish the variety.
7.1 Resistance to pests and diseases
7.2 Special conditions for testing the variety
7.2.1 Day length conditions which favor full bulb
development
(a) short day
[ ]
(b) long day
7.2.2 Dry matter content
(a) low
[ ]
(b) medium
[ ]
(c) high
[ ]
[ ]
Suitability for storage
(a) none
[ ]
(b) short term
[ ]
(c) long term
Other information
8.
[ ]
Authorization for release
(a) Does the variety require prior authorization for release under legislation concerning the
protection of the environment, human and animal health?
Yes
[ ]
No
[ ]
(b) Has such authorization been obtained?
Yes
[ ]
No
[ ]
If the answer to that question is yes, please attach a copy of such an authorization.
end of document
146
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Tomato (Lycopersicon esculentum Mill.)
I.
Coverage of These Guidelines
The Test of Guidelines described in this publication for the conduct of
distinctness, uniformity and stability tests (DUS) shall apply to all varieties of tomato
(Lycopersicon esculentum Mill. syn Lycopersicon lycopersicon (L.) Karstenex Farw.).
II.
Plant Propagation Material Requirement
Upon application, 3,000 viable and high quality seeds are to be submitted to the
Plant Variety Protection Office (PVPO) together with the application. Applicants outside
the Philippines must make sure that all custom formalities regarding seed
exportation/importation are complied with.
The PVP office will determine the additional amount of seeds to be submitted to
the PVP office for the substantive examinations or comparative growing trial. The
minimum requirement, however, is 10g or 3,000. Seeds must have at least 90%
germination, 99% purity, at most 10% moisture content, vigorous, and free from pest or
disease
The seeds should not have undergone any form of treatment unless otherwise
allowed or requested by the PVP Board in certain circumstances. In such cases, the details
of the seed treatment must be clearly described and declared.
III.
Conduct of Tests
The minimum duration of the DUS test should be at least two similar growing
periods i.e. two dry or two wet seasons. The test should be conducted in at least one
location or at least in one accredited DUS Testing Center to be determined by the PVP
Board.
The comparative growing trial must be carried out under conditions that would
ensure normal growth of the plants. The size of the plots should be such that plants or
parts of plants may be removed for measurement and counting without prejudice to the
observations which must be made up to the end of the growing period. Each trial or test
should have at least two replications. The minimum total number of plants to be tested
for each comparative growing trial is 20 plants for greenhouse evaluation and 40 plants
for open field trials. Separate plots for observation and for measurement purposes can
only be used if the growing and environmental conditions are similar.
All test varieties must be treated uniformly i.e. water or irrigation, insecticide,
fungicide, and others following the requirement of National Cooperative Trials (NCT)
cultural management recommendations.
147
Plant Variety Gazette - Volume 2 Issue 1
IV.
Test Guidelines
Methods and Observations
Unless otherwise indicated, all observations determined by measurement,
weighing or counting should be made on 20 plants or parts taken from each of 20 plants.
For the assessment of uniformity, a population standard of one percent (1%) and
an acceptance probability of at least ninety-five (95%) should be applied. In the case of a
sample size of 20 plants, the maximum number of off-types allowed would be one (1) In
the case of a sample size of 40 plants, the maximum number of off-types would be two (2).
When the resistance characteristics are used for assessing distinctness, uniformity
and stability, records must be taken under conditions of controlled infection and, unless
otherwise specified, on at least 10 plants.
All observations on the leaf should be made before ripening of the first fruit
cluster.
V.
Grouping of Varieties
To facilitate the assessment of distinctness, the varieties for evaluation should be
divided into groups. Characteristics which are suitable for grouping purposes are those
with are known from experience not to vary, or to vary only slightly, within a variety.
Their various states of expression should be fairly evenly distributed throughout the
collection.
It is recommended that the following characteristics be used for grouping
varieties:
Plant: growth type (characteristic 2)
Maturity (duration of harvest)
VI.
Characteristics and Symbols
The test for distinctness, uniformity and stability should be based on the
characteristics and their state of expressions as listed in the Table of Characteristics (next
chapter).
For the purpose of electronic data processing, notes (numbers) are given opposite
the states of the different characteristics.
Following are the legend/description of the symbols used in the Table of
Characteristics:
(*) refers to characteristics that should be used on all varieties in every growing
period over which examinations are made and always included in the variety
descriptions, except when the state of expression of a preceding characteristic or certain
environmental condition would render this impossible
(+) See explanations on the Table of Characteristics in Chapter VIII.
148
Plant Variety Gazette - Volume 2 Issue 1
VII.
Trait
No.
1.
(*)
2.
(*) (+)
3.
4.
(+)
5.
(+)
6.
(*)
7.
(*)
Test Guidelines
Table of Characteristics for Tomato (Lycopersicon esculentum Mill.)
Characteristic
Seedling: anthocyanin
coloration of hypocotyls
Plant: growth type
Only determinate growth type
varieties:
Plant: number of inflorescences
on main stem (sideshoots to be
removed)
Stem: anthocyanin coloration of
upper third
Only indeterminate growth
type of varieties:
Stem: length of internode
(between 1st and 4th
inflorescence)
Leaf: attitude/orientation in
middle third of plant
Leaf: length
(indicate range for each category)
Description/
Category
Reference
(Example) Varieties
Note/
Remarks
absent
1
present
9
determinate
1
indeterminate
2
few
3
medium
5
many
7
absent or very
weak
weak
1
medium
5
strong
7
very strong
9
short
3
medium
5
long
7
semi-erect
3
horizontal
5
semidrooping
short
7
medium
5
long
7
149
3
3
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
8.
(*)
9.
(*)
10.
(+)
11.
12.
13a.
13b.
14.
Characteristic
Leaf: width
(indicate range for each category)
Leaf: division of blade
Leaf: size of leaflets (in middle
of leaf)
Leaf: intensity of green color
Leaf: glossiness (as for 6)
Leaf: blistering (as for 6)
Leaf: blistering if present
(as for 6)
Leaf: size of blisters (as for 6)
Test Guidelines
Description/
Category
Reference
(Example) Varieties
Note/
Remarks
narrow
3
medium
5
broad
7
pinnate
1
bipinnate
2
very small
1
small
3
medium
5
large
7
very large
9
light
3
medium
5
dark
7
weak
3
medium
5
strong
7
absent
1
present
9
weak
3
medium
5
strong
7
small
3
medium
5
large
7
150
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
15.
(+)
16.
17.
18.
(+)
19.
(*)
20.
(*) (+)
21.
(*) (+)
22.
(*)
Characteristic
Leaf: attitude/orientation of
petiole of leaflet in relation to
main axis (as for 6)
Inflorescence: type (2nd and 3rd
truss)
Flower: fasciation (first flower
of inflorescence)
Flower: pubescence of style
Flower: color
Peduncle: abscission layer
Only for varieties with
abscission layers
Peduncle: length (from
abscission layer to calyx)
Fruit: size (individual fruit
weight in grams)
Test Guidelines
Description/
Category
Reference
(Example) Varieties
Note/
Remarks
semi-erect
3
horizontal
5
semidrooping
mainly
uniparous
intermediate
7
mainly
uniparous
absent
(sessiled)
present
(pediceled)
absent or very
scarce
present
3
yellow
1
orange
2
absent
1
present
9
short
3
medium
5
long
7
very small
1
small
3
medium
5
large
7
very large
9
151
1
2
1
9
1
9
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
23.
(*)
24.
(*) (+)
25.
(*)
Characteristic
Fruit: ratio
Length/diameter
Fruit: shape in longitudinal
section
Fruit: ribbing at peduncle end
Test Guidelines
Description/
Category
very small
Reference
(Example) Varieties
Note/
Remarks
1
small
3
medium
5
large
7
very large
9
flattened
1
slightly
flattened
circular
2
rectangular
4
cylindrical
5
elliptic
6
heart-shaped
7
obovate
8
ovate
9
pear-shaped
10
absent or very
weak
weak
1
medium
5
strong
7
very strong
9
152
3
3
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
26.
27.
(+)
28.
29.
Characteristic
Fruit: cross section
Fruit: depression at peduncle
end
Fruit: size of peduncle scar
Fruit: size of blossom scar
Test Guidelines
Description/
Category
Reference
(Example) Varieties
Note/
Remarks
not round
1
round
2
medium
5
large
7
very large
9
absent or very
weak
weak
1
medium
5
strong
7
very strong
9
very small
1
small
3
medium
5
large
7
very large
9
very small
1
small
3
medium
5
large
7
very large
9
153
3
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
30.
(+)
31.
32.
33.
(*)
Characteristic
Fruit: shape at blossom end
Fruit: size of core in cross
section (in relation to total
diameter)
Fruit: thickness of pericarp
Fruit: number of locules
Test Guidelines
Description/
Category
Reference
(Example) Varieties
Note/
Remarks
Indented
1
indented to
flat
flat
2
flat to pointed
4
pointed
(Slight)
pointed
(Medium)
highly Pointed
5
very small
1
Small
3
Medium
5
Large
7
very large
9
Thin
3
medium
5
thick
7
only two
1
two or three
2
three or four
3
four, five or
six
more than six
4
154
3
7
9
5
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
34.
(*)
35.
(*)
36.
(*)
37.
(*)
38.
(*)
39.
(*)
Characteristic
Fruit: green shoulder before
maturity
Fruit: extent of green shoulder
(as for 34)
Fruit: intensity of green color of
shoulder (as for 34)
Fruit: intensity of green color
(as for 34)
Fruit: color at maturity (vine
ripe)
Fruit: color of flesh (at
maturity)
(vine ripe)
Test Guidelines
Description/
Category
Reference
(Example) Varieties
Note/
Remarks
absent
1
present
9
small
3
medium
5
large
7
light
3
medium
5
dark
7
light
3
medium
5
dark
7
cream
1
yellow
2
orange
3
pink
4
red
5
brownish
6
cream
1
yellow
2
orange
3
pink
4
red
5
brownish
6
155
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
40.
(*) (+)
41.
(+)
42.
(+)
43.
(*)
44.
Characteristic
Fruit: firmness
Fruit: shelf life (under ambient
condition; average no of days
from harvest to 50% of the
fruits still marketable)
Time of flowering (from
sowing)
Time of maturity (from sowing)
Fruit: Total soluble Solid at
maturity
Test Guidelines
Description/
Category
Reference
(Example) Varieties
Note/
Remarks
very soft
1
soft
3
medium
5
firm
7
very firm
9
very short
1
short
3
medium
5
long
7
very long
9
early
3
medium
5
late
7
very early
1
early
3
medium
5
late
7
very late
9
low
3
medium
5
high
7
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Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
45.
46.
47.
48.
49.
50.
Characteristic
Fruit: pH of ripe fruit
Fruit: glossiness
(mature green stage)
Fruit: number/cluster
(from 3rd truss)
Fruit: gel (vine ripe)
Fruit size uniformity/cluster
(from 3rd truss)
Fruit: cracking
Test Guidelines
Description/
Category
Reference
(Example) Varieties
Note/
Remarks
low
3
medium
5
high
7
weak
3
medium
5
strong
7
few
3
intermediate
5
many
7
yellow
1
orange
2
red
3
green
4
variable
(<49% fruits
are of same
size
moderately
uniform (5089% fruits are
of same size)
highly
uniform (at
least 90%
fruits are of
same size)
absent
3
concentric
2
radial
3
157
5
7
1
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
50.1
51
52.
(+)
53.
(+)
54
(+)
54.1
(*)
54.2
(*)
55.
(+)
56.
(+)
Characteristic
Fruit: degree of cracking
Fruit: blotchy ripening
Sensitivity to silvering
Resistance to nematode
(Meloidogyne incognita)
Resistance to Fusarium
oxysporum f. sp. lycoperscici
Race 0 (ex 1)
Race 1 (ex 2)
Resistance to Fusarium
oxysporum f. sp. radicis
lycoperscici
Resistance to leaf mold
(Cercospora fuligena)
Test Guidelines
Description/
Category
Reference
(Example) Varieties
Note/
Remarks
slight
1
medium
3
medium to
severe
severe
4
absent
1
present
9
insensitive
1
sensitive
9
absent
1
present
9
absent
1
present
9
absent
1
present
9
absent
1
present
9
absent
1
present
9
158
5
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
57.
(+)
58.
(+)
Characteristic
Resistance to Tomato Mosaic
Virus
Resistance to late blight
(Phytophthora infestans)
Test Guidelines
Description/
Category
Reference
(Example) Varieties
Note/
Remarks
no virus infection
(absent = highly
resistance)
0
1-10% virus
infection
(resistant)
11-20% virus
infection)
(moderately
resistant)
21-50% virus
infection
(moderately
susceptible)
51-75% virus
infection
(susceptible)
76-100% virus
infection
(highly
susceptible)
no symptom
(absent = highly
resistant)
1-10% of leaf area
affected
(highly resistant)
11-20% of leaf
area affected
(moderately
resistant)
21-40% of leaf
area affected
(resistant)
41-70 of leaf area
affected
(moderately
susceptible)
71-90% of leaf
area affected
(susceptible)
91-100% of leaf
area affected
(highly
susceptible)
1
159
2
3
4
5
0
1
2
3
4
5
6
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
59.
(+)
60.
(+)
61.
(+)
62.
(+)
Characteristic
Resistance to gray leaf spot
(Stemphylium spp.)
Resistance to Bacterial Leaf
Spot (Pseudomonas syringe
pv. tomato)
Resistance to Ralstonia
solanacearum – Race 1
Resistance to Tomato Yellow
Leaf Curl Virus
Test Guidelines
Description/
Category
absent
Reference
(Example) Varieties
Note/
Remarks
1
present
9
absent
1
present
9
80-100% survival
(resistant)
1
60-79% survival
(moderately
resistant)
2
30-59% survival
(moderately
susceptible)
3
0-29% survival
(susceptible)
4
no virus infection
(absent=highly
resistant)
0
1-10% virus
infection
(resistant)
1
11-20% virus
infection
(moderately
resistant)
2
21-50% virus
infection
(moderately
susceptible)
3
51-75% virus
infection
(susceptible)
4
76-100% virus
infection
(highly
susceptible)
5
160
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
63.
(+)
64.
(+)
65.
(+)
66.
(+)
Characteristic
Resistance to Leveilulla
taurica
Resistant to Oidium
Lycopersicon
Resistance to bacterial spot
(Xanthosmonas campestris pv.
vesicatoria)
Reaction to blossom end rot
(BER)
Test Guidelines
Description/
Category
absent
Reference
(Example) Varieties
Note/
Remarks
1
present
9
absent
1
present
9
absent
1
present
9
absent
1
severe
9
161
Plant Variety Gazette - Volume 2 Issue 1
VIII.
Test Guidelines
Explanation on the Table of Characteristics
Trait No. 2
Plant: growth type
The growth type is predominantly controlled by one monoallelic gene (self-pruning + /
self pruning 1).
Determinate (1): This type is predominantly controlled by the recessive allele, selfpruning – (Sp-). This type produces a limited number of trusses. The number of trusses is
different among plants and is influenced by agroclimatic conditions. In this type, the number of
leaves or internodes between inflorescences varies from one to three. In the terminal truss, the
stem ends with an inflorescence and no lateral shoots are produced.
This type includes some “semi-determinate” varieties which do not have three leaves or
internodes consistently between inflorescences, and show semi-determinate growth, fro example,
with the termination of the stem prolongation above 9th inflorescence (e.g. “Local Variety” type) or
at higher than 20th inflorescence (e.g. “Local Variety” type).
Indeterminate (2): This type is predominantly controlled by the dominant allele, selfpruning + (Sp+). In this type, three leaves or internodes are generally observed between
inflorescence. Each tress produces three buds, the terminal bud is transformed into a flowering
bud; one of the two axillary buds is transformed into a lateral shoot which produces next three
buds and carries on the prolongation of the stem. Plants of this type grows with the continuous
repeat of this growth pattern.
It should be noted that only two leaves or internodes might be observed between
inflorescences in some parts of plants in a certain group of indeterminate variety types (e.g.
varieties originated from “Local Variety”.
Local Varieties, types might be considered to be categorized into an intermediate class
between indeterminate and determinate, but they always have three leaves or internodes between
inflorescences. They should therefore be categorized into the indeterminate type.
Trait No. 4
Stem: anthocyanin coloration of upper third
Most of the varieties are classed 1 to 5. Expression of anthocyanin is influenced by day
temperature. Under greenhouse conditions, the variation is rather low, except for varieties with
Tm2 allele which is linked to anthocyanin of the stem (specially at the internode).
Trait No. 5
Stem length of internode between 1st and 4th inflorescence
(only for indeterminate growth type varieties)
Indeterminate growth type varieties have, in general, 3 nodes between inflorescences
except some genotypes (see Trait No. 2). This means that, in general, there are 12 internodes
between the 1st and 4th inflorescence.
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Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Measure the length between the 1st and 4th inflorescence, and count the number of
internodes (generally 12). In order to obtain the average length of an internode, calculate the
ration: length of stem/number of internodes. The observation should be made at the following
stage:
one leaf above the 5th or 6th inflorescence on staked open field crops
one leaf above the 7th to 12th inflorescence for plants grown in a greenhouse, depending on the
height of the greenhouse
Trait No. 10
Leaf size: size of leaflets (in middle of leaf)
Trait No. 15
Leaf: attitude of petiole of leaflet in relation to main axis
3
5
semi-erect
7
horizontal
semi-drooping
Trait No. 18
Flower: pubescence of style
Some non-hairy varieties can present some rare and small hairs at the base of the style.
163
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Trait No. 20
Peduncle: abscission layer
absent
present
Some varieties which have only a collar of an abscission layer (heterozygous for the gene
which controls the presence of the joint) are considered as jointless (“absent (1)”).
Trait No. 21
Only for varieties with abscission layer: peduncle: length (fro abscission layer to calyx).
Trait No. 24
Fruit: shape in longitudinal section
1
flattened
2
slightly flattened
164
3
circular
Plant Variety Gazette - Volume 2 Issue 1
4
rectangular
Test Guidelines
5
cylindrical
8
obovate
6
elliptic
9
7
heart -shaped
10
ovate
pear-shaped
Trait No. 27
Fruit: depression at peduncle end
1
absent or very weak
3
weak
5
medium
165
9
strong
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Trait No. 30
Fruit: shape at blossom end
1
indented
2
indented to flat
3
flat
4
flat to pointed
5
pointed
Trait No. 40
Fruit Firmness
Method:
Harvesting stage: fruits should be harvested when they are completely colored.
Determining firmness: determine by hand the firmness of the fruits compared to the standard
varieties.
Trait No. 41
Fruit: shelf-life
The length of shelf life is estimated by the number of weeks or days that the fruit remains
commercially viable on the shelf.
Twenty fruits per plot (2 per plant) are picked from the 4th, 5th or 6th cluster in similar
stages of exterior ripening (when green color disappears in half of whole fruit). Fruits are stored
in boxes in stackable plastic trays in single layers. The boxes can be stored one on top of another if
they permit the air to circulate between them. The storage place must be at ambient condition under
low elevation.
Observation is made twice a week based on firmness, shivering, and rotting of fruits until 50%
of fruits are non-marketable. A fruit is considered no longer marketable if the rating for softness is
lower or equal to “3” (see characteristic number 40 in chapter 7). The length of shelf life is
calculated as the number of days from picking to the time when the fruit firmness is no longer
marketable.
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Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
Trait No. 42
Time of Flowering and Maturity
For semi-indeterminate or indeterminate varieties that need stakes or trellis, the time to
flowering and maturity is assessed by recording the flowering date and first harvest from sowing of
the third flower on the second and third trusses on individual plants. It is not recommended to
record the time of flowering in the first trusses since its expression of flowering is more
influenced by the seed vigor and the plantation quality. Obtain the average of the number of
days to flowering.
For determinate varieties, it is strongly advisable to grow them with stakes or trellis and
to record the flowering date in the same manner described above.
Trait No. 44 and 45
Fruit: Total Soluble Solids and Ph
The total soluble solids of freshly squeezed juice from 20 fruits ripe fruits will be taken
using a hand refractometer and recorded as percent degree Brix. The pH will be taken using a pH
meter.
Trait No. 46 to 60
Resistance to Various Diseases
The protocol used for disease screening and evaluation for resistance (either seeding or
field-grown) must be fully described. As much as possible, use known varieties with known level
of resistance and susceptibility as check varieties. The isolate used in the screening or evaluation
must also be described. For seeding inoculation/screening, sterilized soil mix must be used and
conditions for disease development must be recorded for each screening period particularly if the
screening could not be done under controlled conditions such as in the use of growth chambers.
If evaluation or screening is done in multiple sites or locations, the date must for each
location must be summarized and presented in relation to the check varieties as well as the
environmental conditions during the screening.
IX.
Literature:
UPOV TG/44/10. 2001. Guidelines for the conduct of tests for distinctness, uniformity
and stability for tomato (Lycopersicon lycopersicum (L.) Karsten ex. Farw.). International Union for
the Protection of New Varieties of Plants (UPOV), 49 p.
AG:ABPGR/80/103. 1981. Genetic Resources of Tomatoes and Wild Relatives: A Global
report by JT Esquinas-Alcazar. International Board for Plant Genetic Resources (IBPGR), 65 p.
167
Plant Variety Gazette - Volume 2 Issue 1
X.
Test Guidelines
Technical Questionnaire
Reference Number :
(not to be filled in by the applicant)
TECHNICAL QUESTIONNAIRE
(To be completed in connection with an application for plant variety protection)
1. Species:
Lycopersicon esculentum Mill
TOMATO
2. Name and address of applicant:
3. Proposed denomination or breeders reference:
4. Information on origin, maintenance and reproduction of the variety
Method of breeding/type of material :
(i) seed propagagation
- hybrid
- open-pollinated
[ ]
[ ]
[ ]
Other information
5. Characteristics of the variety to be given (the number in brackets refers to the corresponding
characteristics in the DUS Test Guidelines and please mark the state of expression which best
corresponds).
Trait
No.
5.1
(2)
5.2
(9)
Characteristic
Plant: growth type
Leaf: division of blade
Description/Category
Reference
(Example) Varieties
Note/
Remarks
determinate
1[ ]
indeterminate
2[ ]
pinnate
1[ ]
bipinnate
2[ ]
168
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
5.3
(20)
5.4
(22)
5.5
(24)
5.7
(33)
5.8
(34)
Characteristic
Peduncle: abscission layer
Fruit: size
Fruit: length in longitudinal
section
Fruit: number of locules
Fruit: green shoulder before
maturity
Test Guidelines
Description/Category
Reference
(Example) Varieties
Note/
Remarks
absent
1[ ]
present
9[ ]
very small
1[ ]
small
3[ ]
medium
5[ ]
large
7[ ]
very large
9[ ]
flattened
1[ ]
slightly flattened
2[ ]
circular
3[ ]
rectangular
4[ ]
cylindrical
5[ ]
elliptic
6[ ]
heart-shaped
7[ ]
obovate
8[ ]
ovate
9[ ]
pear-shaped
10 [ ]
only two
1[ ]
two or three
2[ ]
three or four
3[ ]
four, five or six
4[ ]
more than six
5[ ]
absent
1[ ]
present
9[ ]
169
Plant Variety Gazette - Volume 2 Issue 1
Trait
No.
5.9
(38)
5.10
(40)
Characteristic
Test Guidelines
Description/Category
Fruit: color at maturity
Fruit: firmness
Reference
(Example) Varieties
Note/
Remarks
cream
1[ ]
yellow
2[ ]
orange
3[ ]
pink
4[ ]
red
5[ ]
brownish
6[ ]
very soft
1[ ]
soft
3[ ]
medium
5[ ]
firm
7[ ]
very firm
9[ ]
Similar varieties and differences from these varieties
Denomination of
similar variety1
Characteristic in
which the similar
variety is different2
State of expression
of similar variety
1 Check
State of expression
of candidate variety
variety from which the comparison was made
In the case of identical states of expression of both varieties, please indicate the size of the
difference.
2
170
Plant Variety Gazette - Volume 2 Issue 1
Test Guidelines
7. Additional information which may help to distinguish the variety
Resistance to pests and diseases
Organism
Strain
(53) Root knot nematode (Meloidogyne incognita)
(54) Resistance to Fusarium oxysporum
f. sp. lycopescici
(55) Resistance to Fusarium oxysporum
f. sp. radicts lycoperscici
(56) Resistance to leaf mold (Cercospora fuligena)
(57) Tomato Mosaic Virus
(58) Late blight (Phythophthora infestans)
(59) Gray leaf spot (Stemphylium spp)
(60) Bacterial leaf spot (Pseudomonas syringae pv.
Tomato)
(61) Bacterial wilt (Ralstonia solanacearum)
(62) Tomato Yellow Leaf Curl Virus
(63) Leveilulla taurica
(64) Oidium lycopersicum
(65) Bacterial spot (Xanthomonas campestris
pv.vVesicatoria)
(66) Blossom end rot
Race 1
Absent
Present
[ ]
[ ]
[ ]
[ ]
Not
tested
[ ]
[ ]
[ ]
[ ]
[ ]
[
[
[
[
[
]
]
]
]
]
[
[
[
[
[
]
]
]
]
]
[
[
[
[
[
]
]
]
]
]
[
[
[
[
[
]
]
]
]
]
[
[
[
[
[
]
]
]
]
]
[
[
[
[
[
]
]
]
]
]
[ ]
[ ]
[ ]
Others (Please specify)
[ ]
[ ]
[ ]
[ ]
[ ]
[ ]
Special conditions for the examination of the variety
Type of culture
- in the greenhouse
- in the open
- with trellis
- without trellis
Main Use
- Fresh market or table
- Industrial (please specify)
- Pot plant
[
[
[
[
]
]
]
]
[ ]
[ ]
[ ]
or processing
Other conditions
Other information
[end of document]
171
PLANT VARIETY PROTECTION OFFICE
2nd Floor, National Seed Quality Control Services Building
Bureau of Plant Industry
Visayas Avenue, Diliman, Quezon City
Telefax: (02) 929-2543
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