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Prof. Gerald H. Pollack
University of Washington, USA
“Professor Haruo Sugi continues his valuable tradition of regular compendia of important reviews
by leading experts in the field of muscle research. The reviews cover skeletal, cardiac and smooth
muscle as well as cell motility. Some of the reviews are didactic, while others shed new light on
old concepts. The volume is packed with useful, in-depth information for the newcomer and the
expert alike.”
Dr. Pradeep Luther
Imperial College London, UK
“For over more than two decades, the muscle symposia organized by Prof. Haruo Sugi have
allowed both young and seasoned muscle scientists to confront new data and new hypotheses. The
present book includes a fascinating chapter by Sugi himself where the fundamental event of the
myosin power stroke is studied afresh with a new powerful EM technique.”
Prof. Jean-Marie Gillis
University of Louvain, Belgium
“It is widely believed that the mechanisms of muscle contraction and its regulation have been
completely understood, but the reality is quite different, as highlighted in this book. Although
the sliding mechanism of contraction and the regulatory role of Ca2+ have been established,
the structural changes of the protein assembly responsible for these mechanisms have not been
elucidated yet. This book concretely indicates where the future challenges lie concerning the
mechanisms of muscle contraction and motility of non-muscle cells.”
Prof. Shin’ichi Ishiwata
Waseda University, Japan
This book provides a comprehensive overview of the current progress in muscle contraction
and cell motility research. It discusses structural, mechanical, and biochemical characteristics
of skeletal, cardiac, and smooth muscles, and cell motility. The experimental objects of the
studies described in this volume extend from humans to molecules. A distinct feature of this
volume is that, in some chapters, evidence against the textbook view is presented, showing
how well-established dogma can be denied by an unexpected discovery. This book is as
interesting as it is informative for general readers and young scientists alike, and it is sure to
inspire both to challenge the enticing mysteries that still remain in this exciting research field.
V531
ISBN 978-981-4745-16-1
Sugi
Haruo Sugi graduated from the Postgraduate School of the University
of Tokyo, Japan, with a PhD in 1962 and was appointed instructor in the
Department of Physiology of the University of Tokyo. From 1965 to 1966, he
worked at Columbia University as a research associate and at the National
Institutes of Health as a visiting scientist. He was a professor in and chairman
of the Department of Physiology, Teikyo University Medical School, Japan,
from 1973 to 2004, when he became emeritus professor.
Muscle Contraction and Cell Motility
“Sugi’s book provides an extensive collection of the latest and most up-to-date aspects of the field
of muscle contraction from the leaders of the field. Especially appealing are the frank critiques
of the current models, which provide abundant food for thought. I heartily encourage anyone
interested in the field to devour the contents of this informative book.”
Muscle Contraction
and Cell Motility
Fundamentals and Developments
edited by
Haruo Sugi
Muscle Contraction
and Cell Motility
Muscle Contraction
and Cell Motility
Fundamentals and Developments
editors
Preben Maegaard
Anna Krenz
Wolfgang Palz
edited by
Haruo Sugi
The Rise of Modern Wind Energy
Wind Power
for the World
Published by
Pan Stanford Publishing Pte. Ltd.
Penthouse Level, Suntec Tower 3
8 Temasek Boulevard
Singapore 038988
Email: [email protected]
Web: www.panstanford.com
British Library Cataloguing-in-Publication Data
A catalogue record for this book is available from the British Library.
Muscle Contraction and Cell Motility: Fundamentals
and Developments
Copyright © 2017 Pan Stanford Publishing Pte. Ltd.
All rights reserved. This book, or parts thereof, may not be reproduced in any
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ISBN 978-981-4745-16-1 (Hardcover)
ISBN 978-981-4745-17-8 (eBook)
Printed in the USA
Contents
Preface
Part I: Skeletal Muscle
xvii
1. Electron Microscopic Visualization and Recording of
ATP-Induced Myosin Head Power Stroke Producing Muscle
Contraction Using the Gas Environmental Chamber
3
Haruo Sugi, Tsuyoshi Akimoto, Shigeru Chaen, Takuya Miyakawa,
Masaru Tanokura, and Hiroki Minoda
1.1 Historical Background
1.2 Materials and Methods
1.2.1 The Gas Environmental Chamber
1.2.2 Carbon Sealing Film
1.2.3 Iontophoretic Application of ATP
1.2.4 Determination of the Critical Electron Dose
Not to Impair Physiological Function of the
Specimen
1.2.5 Position-Marking of Myosin Heads with
Site-Directed Antibodies
1.2.6 Recording of Specimen Image and Data
Analysis
1.3 Myosin Head Movement Coupled with ATP
Hydrolysis in Living Myosin Filaments in the
Absence of Actin Filaments
1.3.1 Stability in Position of Individual Myosin
Heads in the Absence of ATP
1.3.2 Amplitude of ATP-Induced Myosin Head
Movement in Hydrated Myosin Filaments
1.3.3 Reversal in Direction of ATP-Induced
Myosin Head Movement across Myosin
Filament Bare Zone
4
9
9
10
11
11
12
15
15
15
16
18
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Contents
1.3.4 Reversibility of ATP-Induced Myosin
Head Movement
19
1.3.6 Summary of Novel Features of ATP-Induced
Myosin Head Movement Revealed by
Experiments Using the EC
21
1.3.5 Amplitude of ATP-Induced Movement at
Various Regions within a Myosin Head
1.4 Novel Features of Myosin Head Power Stroke in
the Presence of Actin Filaments
1.4.1 Preparation of Actin and Myosin
Filament Mixture
1.4.2 Conditions to Record ATP-Induced Myosin
Head Power Stroke in the Filament Mixture
1.4.3 Amplitude of ATP-Induced Myosin Head
Power Stroke in the Mixture of Actin and
Myosin Filaments
1.4.4 Reversibility of ATP-Induced Myosin Head
Power Stroke
1.4.5 Summary of Novel-Features of ATP-Induced
Myosin Head Power Stroke Revealed by
Experiments Using the EC
2. Studies of Muscle Contraction Using X-Ray Diffraction
20
22
22
23
25
29
30
35
John M. Squire and Carlo Knupp
2.1 Introduction
2.4 Meridional Reflections
59
2.6 Conclusion
67
2.2 Basic Concepts in Diffraction
2.3 Equatorial Reflections
2.5 The Full 2D Diffraction Pattern: Identifying
Structural Mechanisms
3. Muscle Contraction Revised: Combining Contraction
Models with Present Scientific Research Evidence
Else Marie Bartels
3.1 Introduction
36
42
48
64
75
76
Contents
3.2 Findings and Facts That Must Be Part of—or
Explained by—a Model for Contraction
3.2.1 Structure of the Contractile Apparatus
3.2.1.1 General structure
3.2.1.2 Proteins making up the
contractile unit
3.2.2 The Internal Environment in a Muscle Cell
3.2.3 Energy Consumption During Contraction
3.2.3.1 ATP consumption and ATPase
rates during contraction
3.2.3.2 Electric charge changes
initiated by ATP
3.2.4 Active Force Development
3.2.5 Stiffness and General Elastic Properties
of the Contractile Unit
3.3 The Dynamic Contractile Unit
3.3.1 What Happens during a Contraction?
3.3.2 Importance of Considering Ion
Movements as the Base for Contraction
3.4 Conclusions
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97
102
102
4. Limitations of in vitro Motility Assay Systems in
Studying Molecular Mechanism of Muscle Contraction
as Revealed by the Effect of Antibodies to Myosin Head 117
Haruo Sugi, Shigeru Chaen, Takuya Miyakawa, Masaru Tanokura,
and Takakazu Kobayashi
4.1
4.2
4.3
4.4
4.5
80
80
80
Introduction
Historical Background
Development of in vitro Motility Assay Systems
In vitro Force-Movement Assay Systems
Properties of Three Antibodies Used to
Position-Mark Myosin Heads at Different
Regions within a Myosin Head
4.6 Different Effects of three Antibodies to Myosin
Head between in vitro Actin–Myosin Sliding
and Muscle Contraction
118
119
122
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129
130
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Contents
4.6.1 Antibody 1 (Anti-CAD Antibody) Has No
Effect on Both in vitro Actin–Myosin
Sliding and Muscle Contraction
4.6.2 Antibody 2 (Anti-RLR Antibody) Inhibits in
vitro Actin–Myosin Sliding, but Has No
Appreciable Effect on Muscle Contraction
4.6.3 Antibody 3 (Anti-LD Antibody) Shows No
Marked Inhibitory Effect on in vitro
Actin–Myosin Sliding, but Has Inhibitory
Effect on Ca2+-Activated Muscle Contraction
4.7 Definite Differences in the Mechanism between
in vitro Actin–Myosin Sliding and Muscle
Contraction as Revealed by the Effect of Antibodies
to Myosin Head
4.7.1 Evidence That Myosin Heads Do Not Pass
through Rigor Configuration during Their
Cyclic Attachment-Detachment with Actin
Filaments
4.7.2 The Finding That Anti-RLR Antibody
Inhibits in vitro Actin–Myosin Sliding but
Not Muscle Contraction Suggests That
Myosin Head Flexibility at the Converter
Domain Is Necessary for in vitro
Actin–Myosin Sliding but Not for Muscle
Contraction
4.7.3 The Finding That Anti-LD Antibody Inhibits
Muscle Contraction but Not in vitro Actin–
Myosin Sliding Suggests that Movement of
the LD Is Necessary for Muscle Contraction
but Not for in vitro Actin–Myosin Sliding
4.8 Conclusion
5. Characteristics and Mechanism(s) of Force Generation
by Increase of Temperature in Active Muscle
K. W. Ranatunga
5.1 Introduction
5.2 Methods and Materials 130
131
133
135
135
136
137
139
143
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Contents
5.2.1 Experimental Techniques and Procedures
5.2.2 Muscle Preparations
5.2.3 Abbreviations, Nomenclature and Data
Analyses
5.3 Temperature Dependence of Steady Force
5.3.1 Isometric Force and Force during
Shortening/Lengthening
5.3.2 Effects of Pi and ADP (Products of
ATP Hydrolysis)
5.4 Tension Response to Temperature-Jump
5.4.1 During Muscle Shortening and
Lengthening
5.4.2 Effects of Pi and ADP on T-Jump Force
Generation
5.4.3 A Minimal Crossbridge Cycle
5.5 Some General Observations
5.5.1 Unresolved Issues
5.5.2 Value of Temperature-Studies
6. Mechanism of Force Potentiation after Stretch in
Intact Mammalian Muscle
Giovanni Cecchi, Marta Nocella, Giulia Benelli, Maria Angela Bagni,
and Barbara Colombini
6.1 Introduction
6.2 Materials and Methods
6.2.1 Animals, Fibre Dissection and
Measurements
6.2.2 Static Tension Measurements
6.3 Results
6.3.1 Static Tension
6.3.2 Effects of Sarcomere Length on Active
and Passive Tension
6.3.3 Effects of Sarcomere Length on Static
Stiffness
6.4 Discussion
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181
ix
Contents
6.4.1 Equivalence between Residual Force
Enhancement and Static Tension
6.4.2 Dependence of Static Stiffness on
Sarcomere Length
6.4.3 BTS Effects
6.4.4 Independence of Static Tension from
Crossbridges
6.4.5 Residual Force Enhancement and Static
Tension Mechanism
6.4.6 Conclusions
7. The Static Tension in Skeletal Muscles and Its
Regulation by Titin
Dilson E. Rassier, Anabelle S. Cornachione, Felipe S. Leite,
Marta Nocella, Barbara Colombini, and Maria Angela Bagni
7.1 Introduction
7.2 Characteristics of the Static Tension
7.3 Mechanisms of Increase in Non-Cross-Bridge
Forces
7.4 Conclusion and Physiological Implications
8. Stiffness of Contracting Human Muscle Measured
with Supersonic Shear Imaging
Kazushige Sasaki and Naokata Ishii
8.1 Introduction
8.2 Methods and Materials
8.2.1 Theoretical Basis of Supersonic Shear
Imaging
8.2.2 Some Technical Issues
8.3 Muscle Activation Level and Stiffness
8.3.1 Association of Shear Modulus with Joint
Torque
8.3.2 Association of Shear Modulus with Motor
Unit Activity
8.3.3 Usefulness as a Measure of Muscle
Activation Level
181
182
183
183
184
186
193
194
194
198
202
209
210
213
213
215
216
216
218
219
Contents
8.4 Relations between Length, Force, and Stiffness
8.4.1 Length-Dependent Changes in Shear
Modulus
8.4.2 Linear Association of Force and Shear
Modulus
8.4.3 Difference between Tetanic and Voluntary
Contractions
8.5 Stiffness Measured during Dynamic Contractions
8.5.1 Differences in Shear Modulus among
Contraction Types
8.5.2 Putative Mechanisms
8.6 General Conclusions and Perspectives
9. Effect of DTT on Force and Stiffness during Recovery
from Fatigue in Mouse Muscle Fibres
Barbara Colombini, Marta Nocella, Joseph D. Bruton,
Maria Angela Bagni, and Giovanni Cecchi
9.1 Introduction
9.2 Methods
9.2.1 Fibre Dissection and Measurements
9.2.2 Force and Stiffness Measurements
9.3 Results
9.4 Discussion
Part II: Cardiac and Smooth Muscle
10. ATP Utilization in Skeletal and Cardiac Muscle:
Economy and Efficiency
219
220
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222
223
223
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226
235
236
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238
239
243
249
G. J. M. Stienen
10.1 Introduction
10.2 The Crossbridge Cycle
10.3 Dependence of ATP Utilization on Activity,
Fiber Type and Species
10.4 ATP Utilization in Cardiac Muscle
10.5 The Fenn Effect
10.6 Future Perspectives
250
252
253
260
262
265
xi
xii
Contents
11. Essential Myosin Light Chains Regulate Myosin
Function and Muscle Contraction
Ingo Morano
11.1 Structure and Interaction Interfaces of
Essential Myosin Light Chains
11.1.1 Structure of Myosin II
11.1.2 Structure of Essential Myosin
Light Chains
11.1.3 ELC Interaction Interfaces
11.1.4 ELC Phosphorylation
11.2 Functional Roles of ELCs?
11.2.1 ELC/MyHC Interactions
11.2.1.1 ELC/lever arm interactions
11.2.1.2 ELC/motor domain couplings
11.2.2 ELC/Actin Interaction
11.2.5 Functional Roles of ELC Isoforms
11.2.3 ELC/RLC Interaction
11.2.4 Phosphorylation of ELC
11.2.5.1 Striated muscle ELC isoforms
11.2.5.2 Smooth muscle ELC isoforms
11.3 Pathophysiology of ELC
12. Regulation of Calcium Uptake into the Sarcoplasmic
Reticulum in the Heart
Susumu Minamisawa
12.1 Introduction
12.4 PLN Mutations Related to Human
Cardiomyopathy
12.2 SERCA2a Plays a Central Role in
Ca2+
Uptake
12.3 Phospholamban: A Critical Regulator of SERCA2a
12.5 Enhancement of SR Function Is a Novel
Therapeutic Target for Heart Failure
12.5.1 Strategies to Increase SERCA2a Protein
in Heart Failure
273
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285
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288
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304
306
307
308
309
310
Contents
12.5.2 Strategies to Modulate SERCA2a to
Increase Ca2+ Transport
12.5.3 Strategies to Decrease PLN Protein in
Heart Failure
12.5.4 Strategies to Disrupt the Interaction
between SERCA2a and PLN
12.6 Sarcolipin, a Homologue of PLN, Is an
Atrium-Specific Inhibitor of SERCA2a
12.7 Sarcalumenin Is a Newly Identified Ca2+-Binding
Glycoprotein That Regulates SERCA2a Stability
and Activity in Mammals
12.8 Conclusion
13. The Pivotal Role of Cholesterol and Membrane Lipid
Rafts in the Ca2+-Sensitization of Vascular Smooth
Muscle Contraction Leading to Vasospasm
Ying Zhang, Hiroko Kishi, Katsuko Kajiya, Tomoka Morita,
and Sei Kobayashi
13.1 Introduction
13.2 SPC Is a Causal Factor of Ca2+-Sensitization
Leading to Vasospasm
13.3 The Signaling Pathway of SPC-Induced
Ca2+-Sensitization Leading to Vasospasm
13.4 Role of Cholesterol and Membrane Lipid Rafts
in SPC-Induced Ca2+-Sensitization Leading to
Vasospasm
13.5 Summary
14. The Catch State of Molluscan Smooth Muscle
Stefan Galler
14.1
14.2
14.3
14.4
14.5
14.6
Background
Structures of Catch Muscles
Regulation of Catch
Catch Theories
Challenges of the Traditional Myosin Head Theory
Twitchin Bridges
311
312
313
315
318
320
333
334
335
336
337
339
343
344
346
347
349
350
352
xiii
xiv
Contents
14.7 Myosin Heads Tied by Twitchin
14.8 Myorod
14.9 Interconnections between Thick Filaments?
14.10 Additional Kinases and Phosphatases
14.11 Catch during Active Contraction
14.12 Conclusion
353
355
356
357
358
359
15. Regulation of Dynein Activity in Oscillatory Movement
of Sperm Flagella
371
Part III: Cell Motility
Chikako Shingyoji
15.1 Introduction
15.2 Basic Features of Flagellar Movement
15.3 Dynein Force Generation and Microtubule
Sliding in the Axoneme
15.4 Control of Microtubule Sliding and Bend
Formation
15.5 Regulation of Dynein Activities by Mechanical
Signal
15.6 Outlook
16. The Biomechanics of Cell Migration
Yoshiaki Iwadate
16.1 Introduction
16.2 The Cytoskeleton
16.2.1 Cytoskeleton and Cell Type
16.2.2 Fundamental Mechanism of Cell
Migration Based on Actin Polymerization
and Actomyosin Contraction
16.2.3 The Role of Microtubules in Maintaining
Anterior-Posterior Polarity
16.2.4 Variety of Cell Migration Mechanisms
16.3 Traction Forces
16.3.1 Traction Forces Exerted by Fibroblasts
16.3.2 Traction Forces Exerted by Dictyostelium
Cells and Neutrophils
372
372
375
380
383
384
387
388
389
390
392
395
396
396
396
397
Contents
16.3.3 Traction Forces Applied by Keratocytes
16.4 Mechanosensing and Cell Migration
16.4.1 Passive Mechanosensing
16.4.2 Active Mechanosensing
16.4.3 Contact Guidance
16.5 Conclusion and Future Perspectives
17. Role of Dynamic and Cooperative Conformational
Changes in Actin Filaments
Taro Q. P. Uyeda
17.1 An Exceptionally Conservative and Multifunctional
Protein: Actin
17.2 Structural Polymorphism of Actin Filaments
17.3 Cooperative Conformational Changes of Actin
Filaments Induced by ABPs
17.3.1 Interaction with Cofilin
17.3.2 Interaction with Myosin
17.3.3 Interaction with Drebrin
17.3.4 Interactions with End-Binding ABPs
17.4 Physiological Roles of Cooperative Polymorphism
of Actin Filaments
17.4.1 Segregation of ABPs along Actin
Filaments
17.4.2 Amplification of the Inhibitory Effect
17.4.3 Intracellular Signaling Wire
17.5 Actin Filaments as Mechanosensors
17.6 Why Is Actin So Conservative?
17.7 Possible Dynamic Roles of Actin Filaments
in Muscle Contraction
Index
398
399
400
403
404
404
415
416
418
419
420
421
423
423
425
427
428
428
428
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445
xv
Preface
This volume provides a comprehensive overview of the current
progress in research on muscle contraction and cell motility, not
only for investigators in these research fields but also for general
readers who are interested in these topics. One of the most
attractive features of living organisms is their ability to move. In
vertebrate animals, including humans, their body movement is
produced by skeletal muscles, which are also called striated muscle
due to their cross-striations. In the mid-1950s, H. E. Huxley and
Hanson made a monumental discovery that the skeletal muscle
consists of hexagonal lattice of two different myofilaments, i.e., actin
and myosin filaments, and that muscle contraction results from
relative sliding between actin and myosin filaments. Concerning
the mechanism of the myofilament sliding, Huxley put forward
a hypothesis that myosin heads (cross-bridges) extending from
myosin filaments first attach to actin, undergo conformational
changes to cause unitary filament sliding, and then detach from
actin, each attachment-detachment cycle being coupled with ATP
hydrolysis. Despite extensive studies having been carried out up to
the present time, the myosin head movement still remains to be a
matter of debate and speculation.
The text is organized into three parts. Part I contains
nine chapters on the current progress in contraction characteristics
and mechanical properties of the skeletal muscle. In Chapter 1,
Sugi et al. describe their recent success in the electron microscopic
recording of the myosin head movement coupled with ATP
hydrolysis by using the gas environmental chamber, which enables
the study of dynamic structural changes of living biomolecules
related to their function. In Chapter 2, Squire and Knupp summarize
the results obtained by using the time-resolved X-ray diffraction
technique, detecting structural changes of myofilaments in
contracting muscle in a non-invasive manner, and point out
problems in interpreting the results. In Chapter 3, Bartels
emphasizes the essential role of ions in muscle contraction, a
topic generally ignored by muscle investigators. In Chapter 4, Sugi
et al. point out that the results obtained from in vitro motility
xviii
Contents
assay systems, in which actin filaments are made to interact with
myosin heads detached from myosin filaments, may bear no
direct relation to myofilament sliding in muscle. In Chapter 5,
Ranatunga discusses the mechanism of force generation in the
muscle based on his temperature-jump experiments. The 3D
myofilament-lattice structure is known to be maintained by a
network of a large protein, titin. Cecchi et al. in Chapter 6 and
Rassier et al. in Chapter 7 show that Ca2+-dependent stiffness
changes of titin play an important role in muscle mechanical
performance. For a full understanding of skeletal muscle
performance in humans, it is useful to measure stiffness of the
contracting human muscle by means of supersonic shear imaging
(SSI). In Chapter 8, Sasaki and Ishii explain the theoretical
background of SSI together with the results obtained from the
contracting human skeletal muscle. In Chapter 9, Colombini et
al. discuss the mechanism underlying muscle fatigue. Readers of
this volume may become aware of discrepancies between what
are stated in some chapters in this part and what are generally
stated in many textbooks. We emphasize that these discrepancies
reflect the general features of science in progress. Well-established
dogmas in a scientific field can be denied by an unexpected
discovery.
Part II consists of three chapters on the cardiac muscle and
two chapters on the smooth muscle. The cardiac muscle also
exhibits cross-striations and plays an essential role in blood
circulation in the animal body. In Chapter 10, Stienen gives an
extensive overview on various factors affecting the rate of ATP
utilization of skeletal and cardiac muscles in a variety of animals,
including humans. In Chapter 11, Morano also gives an extensive
overview on the role of myosin essential light chain in regulating
myosin function in skeletal, cardiac, and smooth muscles,
based on the crystallographic structure of myosin molecule. In
Chapter 12 by Minamisawa deals with proteins involved in Ca2+
cycling in cardiac muscle by citing vast literature in this clinically
important research field. Smooth muscles do not show striations
because of irregular arrangement of myofilaments, though their
contraction mechanism is believed to be similar to that in skeletal
and cardiac muscles. In Chapter 13, Kobayashi discusses factors
that affect vascular smooth muscle diseases, including his recent
interesting finding. Chapter 14, by Galler, is concerned with the
Contents
so-called catch mechanism in the molluscan somatic smooth
muscle, which is highly specialized to maintain tension over a long
period with little energy expenditure.
Finally, Part III contains three chapters on cell motility. In
Chapter 15, Shingyoji presents a comprehensive overview of the
factors that influence the oscillatory movement of cilia and flagella
caused by sliding between dynein and microtubule. In Chapter 16,
Iwadate discusses crawling cell migration, which is caused by actin
polymerization as well as actin–myosin interaction and is involved
in a variety of biological phenomena, including wound healing
and immune system function. In Chapter 17, Uyeda gives an
extensive survey of the research on the role of actin filaments and
actin-binding proteins in producing a wide range of cell activities.
This book constitutes a fascinating collection of overviews
on muscle contraction and cell motility written by first-class
investigators and not only provides information for general readers
about the current progress and controversies in each research
field but also stimulate young investigators to start challenging
remaining mysteries in these exciting research fields.
Haruo Sugi
Tokyo, September 2016
xix