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1239 Victoria St.
Costa Mesa, CA 92627
Main Phone: (888) 773-5959
Fax: (877) 773-5959
Product Information
PRODUCT NAME:
Human Small Intestine Epithelial Cells
CAT NUMBER:
pD015-f
DISEASE STATE:
Normal
QUANTITY:
>5.0 x 105 cells/ml
STORAGE CONDITION:
Store at -196°C (LN2) upon receipt until ready to culture
STORAGE SOLUTION:
10% DMSO in fetal bovine serum
PRODUCT DESCRIPTION:
Human small intestines epithelial cells are derived from whole small intestines that have been
dissociated into single cells and purified in culture. Human small intestine epithelial cells are
from a single donor. These are highly purified epithelial cells as determined by specific epithelial
cell markers. Human small intestines epithelial cells enable researchers to study the role of
epithelium located within the three compartments of the small intestines; the duodenum,
jejunum, and ileum. These cells are ideal for screening drug candidates for their intestinal
absorption potential.
HANDLING INSTRUCTIONS:
Human small intestines epithelial cells are shipped on dry ice. Upon arrival place into -196°C
(LN2) for long term storage or subculture (www.dvbiologics.com) immediately.
REFERENCES:
1.
2.
3.
Day (2006). Epithelial stem cells and tissue engineered intestines. Curr Stem Cell Res
Ther. 1(1):113-20.
Fagerholm (2007). Prediction of human pharmacokinetics--gut-wall metabolism. J
Pharm Pharmacol. 59(10):1335-43..
Hayashi (2007). Mechanistic analysis for drug permeation through intestinal membrane.
Drug Metab Pharmacokinet. 22(2):67-77.
QUALITY CONTROL:
All cell processing is validated under strict protocols with IRB approval (see ethics statement
www.dvbiologics.com). All cells are guaranteed to meet or exceed customer’s expectations
when handled according to instructions.
Viability: > 80%
Passage number: Frozen cells (P0-P2).
Cyopreservation Solution: 10% DMSO in fetal bovine serum .
TEST CONDITIONS:
Cell quality is determined visually after analyzing Gram and Acid Fast stainings. Cells are
grown and passed to determine sterility and mycoplasma for all lots. In addition, viability is
determined prior to and after freezing. All cells are confirmed to be highly pure by either
molecular, cellular or protein assays with their specific markers.
Form QA-MRD0004-29v0000
For Technical Support/Assistance, call 1-888-773-5959
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OTHER INFORMATION:
Human origin
DEVELOPMENT PERIOD:
Pre Natal
PRECAUTIONS AND
DISCLAIMERS:
Warranty
DV Biologics is committed to provide superior products and warrants only and not for any
particular purpose of the purchases, that all products sold will perform according to established
product specifications. Full warranty information for cell products may be requested to our
technical support or sales team at the number provided above.
Product Use/Safety Statement
CELLS ARE FOR RESEARCH USE ONLY. Not approved for human or veterinary use, for
application to humans or animals, or for use in in-vitro diagnostic or clinical procedures.
WARNING: THE ACCOMPANYING CELL PRODUCT CONTAINS HUMAN SOURCE
MATERIAL, TREAT AS POTENTIALLY INFECTIOUS. All Donors are tested for the
presence of HIV-I, Hepatitis B Virus and Hepatitis C Virus. Furthermore, Gram staining and
Acid Staining are performed to rule out bacterial contamination.
Material Safety and Data Sheets (MSDSs)
Can be requested via e-mail or by calling our technical support number provided above. In
addition, the MSDS can be mailed, e-mailed or faxed depending on the requested method for
delivery.
Form QA-MRD0004-29v0000
For Technical Support/Assistance, call 1-888-773-5959
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