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arch esearch RESEARCH MODELS Rats Mice Other rodents BALB/c-nu Immundeficient Mouse Strain name: BALB/cAnNRj-Foxn1nu Type: Mutant inbred mouse Origin: Nijmegen - 2009 Colour and related genotype: Albino mouse, A/A, Tyrc/Tyrc, Tyrp1b/Tyrp1b - MHC: Haplotype H2d Breeding: Animal easy to rear, could be aggressive by handling, Mating scheme: homozygous ♂ x heterozygous ♀ (homozygous ♀ can not suckle pups). Description of our model The first nude mouse appeared in an outbred albino mouse stock at the Ruchill Hospital, Glasgow in 1962. This mutation was transferred to the stabilized genetic background of the inbred BALB/cAnN mouse by backcrossing. The genetic background was selected by MacDowell in 1923 from a stock of outbred albino mice obtained by Bagg in 1913. They were transferred to Snell at F32. This mutation, identified by Dr. GRIST, is an autosomal recessive one and arises in the Foxn1 (forkhead box N1) gene (chromosome 11). It causes thymic aplasia which results in immunodeficiency. Growth curve of BALB/cAnNRj-Foxn1 /Foxn1 This results in a lack of T cells, B cells 35 remain. This mutation also leads to 30 a keratinisation defect of the hair follicule 25 and the epidermis. This results in mice 20 with transient downy hair. When this 15 duvet is gone, mice appear nude. There 10 is no link between the presence of down 5 and immunodeficiency severity. Reproductive data* Genetic background Mating: heterozygous ♀ x homozygous ♂ Inbred: BALB/cAnN Outbred: NMRI Congenic: CB-17 Inbred: NOD Foxn1nu Prkdcscid (chromosome 11) (chromosome 16) Litter size at birth Genotype: Mutation Phenotype: nu Mice * Coat No Yes (albino) T cells No No B cells Functional No Natural Killer Functional Defect on NOD only Complement Functional Defect on NOD only 7 to 8 Mutant pups per litter 2 to 3 Puberty Between 6 and 8 weeks Gestation time Between 18 and 20 days * JANVIER LABS 2011 Data, for an indicative basis Weight in grams nu Comparison of immundeficiency and phenotype of Nude and Scid mutant mice 0 21 28 35 Males Females 42 49 56 63 70 77 84 Age in days n=300 ♂ and 300 ♀ * JANVIER LABS 2017 Data, for an indicative basis Main application and research fields www.janvier-labs.com Experimental infections Immunology Monoclonal antibodies: study and production Our additional offer Labo service Labo service Transgenic service Transgenic service Oncology Transplantation: xenograft and allograft Our added value The « JANVIER LABS Genetic Policy », a specific programme, the homogeneity of the genetic background, identic to the wild types used as controls. Animals with the SOPF standards. A gentling policy for docile and easy-to-handle animals. Optimal stability conditions of our models during shipments, thanks to our dedicated and internal transport service. A scientific support with a team of Veterinarians and PhD. The available scientific bibliography: Research has been conducted, all over the world, from models bred in our laboratories. Discover our updated bibliography of available studies on our Internet website, heading: Customer Support. Laboratory Services Transgenic Services 2017-01-ENG-RM-28