Download Systems that set the standard, software that`s a

AMPLIFICATION
CELL BIOLOGY
CLONING
MICROARRAYS
NUCLEIC ACID
ANALYSIS
PROTEIN FUNCTION
& AN ALYS I S
QUANTITATIVE
PCR
SOFTWARE
SOLUTIONS
Systems that set the standard, software that’s a step ahead.
Lead the way with a Stratagene personal QPCR system
featuring enhanced MxPro™ QPCR Software.
Our Mx3000P® and Mx3005P™ personal quantitative PCR (QPCR) Systems
combine an advanced optical system with powerful data analysis to give you
• Four- or five-color systems to best fit your
research needs
complete flexibility at an affordable price. Our open platform design
supports traditional and advanced applications as well as newly emerging
• 96-well thermal block with temperature
uniformity of +/- 0.25°C
QPCR chemistries. In addition, our MxPro ™ QPCR Software combines leading
edge data analysis algorithms with intuitive organization designed for
ultimate ease-of-use.
Need More Information? Give Us A Call:
Stratagene US and Canada
Order: 800-424-5444 x3
Technical Service: 800-894-1304 x2
Stratagene Japan K.K.
Order: 3-5821-8077
Technical Service: 3-5821-8076
Stratagene Europe
Order: 00800-7000-7000
Technical Service: 00800-7400-7400
www.stratagene.com
®
Mx3000P is a registered trademark of Stratagene in the United States.
Mx3005P™ and MxPro™ are trademarks of Stratagene in the United States.
• New MxPro™ QPCR Software with optional
features for 21 CFR Part 11 compatibility
QUANTITATIVE
PCR
Ct (dR)
QPCR Instrumentation
Log fit values
CYCLO Standards, RSq:0.999
CYCLO, Y = -3.315*LOG(X) + 26.12, Eff. = 100.3%
ENOS Standards, RSq:0.985
ENOS, Y = -3.503*LOG(X) + 37.43, Eff. = 93.0%
CFTR Standards, RSq:0.978
CFTR, Y = -3.242*LOG(X) + 36.33, Eff. = 103.4%
HFE Standards, RSq:0.997
HFE, Y = -3.351*LOG(X) + 35.18, Eff. = 98.8%
ALIEN Standards, RSq:0.989
ALIEN, Y = -4.093*LOG(X) + 31.88, Eff. = 75.5%
38
36
34
32
30
28
26
24
22
20
18
16
1
10
100
Instrument:
+ Excitation: Quartz tungsten-halogen lamp via scanning fiber optics
+
Detector: Single photomultiplier tube (PMT) via scanning fiber optics
+
Filters: Customizable filter wheels with four (Mx3000P® system) or five (Mx3005P™ system)
user-selected filter sets
+
Thermal system: Solid state, Peltier-based, 96-well block thermal system
+
Sample format: Standard 96-well plates, 8-strip tubes, 200-µl tubes, optimized for 25-µl
reactions
+
Embedded computer collects and saves experiment data during power failure or
computer crash
+
Dimensions: 13" (33 cm) W x 18" (46 cm) D x 17" (43 cm) H, Weight: 45 lbs. (20.4 kg)
+
Power: 100 to 230 VAC +/- 10%, 50/60 Hz
Initial Quantities
Figure 1
Five-plex (target) Standard Curves
Three replicates of four-fold dilutions of QPCR Human
Universal Reference cDNA detecting five gene targets
simultaneously. Detection from the highest abundance to the
lowest abundance gene target (CYCLO to ENOS gene targets)
spans a Ct range of 17-37 (delta Ct = 20).
Fluorescence (dRn)
Amplification Plots
0.1
System Performance:
+ Linear dynamic range: 10 orders of magnitude, standard curve method
+
Excitation range: 350 to 750 nm
+
Emission range: 350 to 700 nm
+
Temperature uniformity: +/- 0.25°C at 72°C
+
Thermal block ramp rate: Up to 2.5°C/second
+
Amplification rate: Standard 40-cycle, two-step QPCR reaction completed in 90 minutes;
reaction is completed in 60 minutes using FullVelocity™ SYBR® Green QPCR reagents
(without dissociation curve)
+
Discrimination: Distinguish samples with 5,000 and 10,000 template copies with a
99.7% confidence level
+
Detection down to single-copy equivalents
0.01
2 4 6
8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 44 46 48 50
Cycles
Figure 2
Two-fold Dilution Series
Dilution series from 20,000 to 1.2 copy equivalent of
plasmid containing β-actin detected with a molecular
beacon. Average delta Ct between dilutions is 0.95 cycles.
Log Fold Change to Cal. (Log base 2) (dRn)
Relative Quantity Chart
8
7
6
5
4
3
2
1
0
-1
-2
-3
-4
-5
-6
-7
-8
36-p53
38-p21
40-Fas
42-FasL
44-Bax
46-Bcl
37-p53-1
39-p21-1
41-Fas-1
43-FasL-1
45-Bax-1
47-Bcl-1
Replicate/Well ID
Figure 3
Automated Analysis of Gene Expression Data
Comparative Quantitation module in our MxPro™ Software
automatically calculates relative quantity for gene expression
experiments. In the figure above, fold expression change for
six genes across two treatments is displayed.
Choice of Filters Included
ALEXA Fluor® 350 (350 nm-440 nm)
FAM™/SYBR® Green I (492 nm-516 nm)
TET™ (517 nm-538 nm)
HEX™/JOE™/VIC™ (535 nm-555 nm)
Cy™3 (545 nm-568 nm)
TAMRA™ (556 nm-580 nm)
ROX™/Texas Red® (585 nm-610 nm)
Cy™5 (635 nm-665 nm)
Contact us for more information on our QPCR Systems:
Stratagene US and Canada
Order: 800-424-5444 x3
Technical Service: 800-894-1304 x2
Stratagene Japan K.K.
Order: 3-5821-8077
Technical Service: 3-5821-8076
Stratagene Europe
Order: 00800-7000-7000
Technical Service: 00800-7400-7400
www.stratagene.com
For a list of worldwide distributors, please visit www.stratagene.com
MxPro™ QPCR Software:
+ Interfaces with Mx3000P® and Mx3005P™ Systems simultaneously and supports up to six
systems in any combination attached to a single computer
+
Streamlined plate setup with dye editing dropdown menu
+
Right mouse-click capability throughout the software to allow quicker and easier access to
menu functions
+
The Comparative Quantification module generates and displays normalized fold change
values on a chart using a base 2 logarithmic scale
+
Gene expression fold change is displayed with upper and lower limit error bars based on
replicate variability for up to four targets normalized to a single reference gene with
efficiency corrections for each gene target
+
View amplification plots and standard curves for up to five targets on the same screen with
threshold changes instantly updated on the standard curve
+
Improved text reports display target-specific data analysis parameters at the top of each
report and allow for customization through adding/removing columns and enhanced
sorting capability
+
Export any data set directly into .xls, .ppt, .bmp, .txt, or .xml format. Charts and graphs can
be exported directly to Microsoft® PowerPoint® and Excel®
+
Auto generation of charts and graphs in Excel® during export
+
Create custom consolidated data reports by selecting which analysis screens to include and
how the report will organize the data
+
Optional 21 CFR Part 11 compatible features including secure application login, database
file management, electronic audit trail functionality, and report generation
Mx™ Quantitative PCR Systems:
Mx3005P™ Quantitative PCR System (110v) with notebook computer
Mx3005P™ Quantitative PCR System (110v) with desktop computer
Mx3005P™ Quantitative PCR System (230v) with notebook computer
Mx3005P™ Quantitative PCR System (230v) with desktop computer
Mx3000P® Quantitative PCR System (110v) with notebook computer
Mx3000P® Quantitative PCR System (110v) with desktop computer
Mx3000P® Quantitative PCR System (230v) with notebook computer
Mx3000P® Quantitative PCR System (230v) with desktop computer
MxPro® Software with 21 CFR Part 11 Features
401449
401456
401457
401458
401403
401405
401406
401407
Inquire
ALEXA Fluor® 350 is a registered trademark of Nanoprobes, Inc.
SYBR® are registered trademarks of Molecular Probes.
FAM™, TET™, HEX™, VIC™, JOE™, TAMRA™, and ROX™ are trademarks of Applera Corporation or it's subsidiaries in the US
and certain other countries.
Cy™3 and Cy™5 are trademarks of Amersham Biosciences.
Microsoft®, PowerPoint® and Excel® are registered trademarks of Microsoft Corporation in the United States and/or other countries.
AD 75 (71199)