Download 九十九學年度 生醫系微生物學期末考 姓名： 學號： 謝絹珠教授：40% I

九十九學年度
九十九學年度 生醫系微生物學期末
生醫系微生物學期末考
系微生物學期末考
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學號：
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謝絹珠教授：40%
I. Multiple choice (please choose the most appropriate answer)：40% 每題 1 分
_____1. Septic shock due to gram-positive bacteria is caused by
A) A-B toxins.
B) Lipid A.
C) Membrane-disrupting toxins.
E) Erythrogenic toxin.
D) Superantigens.
_____2. Which of the following does NOT contribute to a pathogen's invasiveness?
A) Toxins
B) Capsule
C) Cell wall
D) Hyaluronidase E) Ligands
_____3. Which of the following statements is false?
A) Leukocidins destroy neutrophils.
B) Hemolysins lyse red blood cells.
C) Hyaluronidase breaks down substances between cells.
D) Kinase destroys fibrin clots.
E) Coagulase destroys blood clots.
_____4. Which of the following statements about exotoxins is generally false?
A) They are more potent than endotoxins.
B) They are composed of proteins.
C) They are not destroyed by heat.
D) They have specific methods of action.
E) They are produced by gram-positive bacteria.
_____5. Endotoxins are
A) Associated with gram-positive bacteria.
B) Specific in their method of action.
C) Part of the gram-negative cell wall.
D) Excreted from the cell.
E) A-B toxins.
_____6. Patients developed inflammation a few hours following eye surgery. Instruments and solutions
were sterile, and the Limulus assay was positive. The patients’ inflammation was due to
A) Bacterial infection.
B) Viral infection.
C) Endotoxin.
_____7. Which organism most easily causes an infection?
A) E. coli O157:H7 ID50=20
B) Legionella pneumophila
D) Treponema pallidum ID50=57
E) Can't tell
_____8. Which of the following statements about M protein is false?
A) It is found on Streptococcus pyogenes.
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D) Exotoxin.
ID50=1
E) Can’t tell.
C) Shigella
ID50=10
B) It is found on fimbriae.
C) It is heat- and acid-resistant.
D) It is readily digested by phagocytes.
E) It is a protein.
_____9. Which of the following organisms does NOT produce an exotoxin?
A) Salmonella typhi
B) Clostridium botulinum C) Corynebacterium diphtheriae
D) Clostridium tetani
E) Staphylococcus aureus
_____10. Poliovirus is ingested and gains access to tissues by which portal of entry?
A) Skin B) Parenteral C) Mucous membranes
D) All of the above
E) None of the above
_____11. Penicillin was considered a "miracle drug" for all of the following reasons EXCEPT
A) It was the first antibiotic.
B) It doesn't affect eukaryotic cells.
C) It inhibits gram-positive cell wall synthesis.
D) It has selective toxicity.
E) It kills bacteria.
_____12. Which of these antimicrobial agents has the fewest side effects?
A) Streptomycin
B) Tetracycline C) Penicillin
D) Erythromycin
E) Chloramphenicol
_____13. Which of the following antimicrobial agents is recommended for use against fungal infections?
A) Amphotericin B B) Bacitracin
C) Cephalosporin
D) Penicillin
E) Polymyxin
Table 20.1
The following data were obtained from a broth dilution test.
Concentration of Antibiotic X
Growth
Growth in
Subculture
2 µg/ml
+
+
10 µg/ml
-
+
15 µg/ml
-
-
25 µg/ml
-
-
_____14. In Table 20.1, the minimal bactericidal concentration of antibiotic X is
A) 2 µg/ml.
B) 10 µg/ml.
C) 15 µg/ml.
D) 25 µg/ml.
E) Can't tell.
_____15. In Table 20.1, the minimal inhibitory concentration of antibiotic X is
A) 2 µg/ml.
B) 10 µg/ml.
C) 15 µg/ml.
D) 25 µg/ml.
E) Can't tell.
_____16. The antibiotic streptomycin binds to the 30S subunit of the ribosome. The effect is to
A) Cause misreading of mRNA in 70S ribosomes.
B) Prevent binding of tRNA in eukaryotes.
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C) Prevent polypeptide elongation in eukaryotes.
D) Prevent peptide bond formation in prokaryotes.
E) Prevent binding of tRNA in eukaryotes.
_____17. Which of the following antibiotics are used to treat fungal infections?
1. Aminoglycosides
2. Cephalosporins
3. Griseofulvin
4. Polyenes
5. Bacitracin
A) 1, 2, and 3
B) 3 and 4
C) 3, 4, and 5
D) 4 and 5
E) All of the antibiotics
_____18. Drug resistance occurs
A) Because bacteria are normal microbiota.
B) When antibiotics are used indiscriminately.
C) Against antibiotics and not against synthetic chemotherapeutic agents.
D) When antibiotics are taken after the symptoms disappear.
E) All of the above.
_____19. Niclosamide prevents ATP generation in mitochondria. You would expect this drug to be
effective against
A) Gram-negative bacteria.
B) Gram-positive bacteria.
D) Mycobacterium tuberculosis.
E) Viruses.
C) Helminths.
_____20. Mebendazole is used to treat cestode infections. It interferes with microtubule formation;
therefore, it would NOT affect
A) Bacteria.
B) Fungi.
C) Helminths.
D) Human cells.
E) Protozoa.
_____21. An 8-year-old girl has scabs and pus-filled vesicles on her face and throat. Three weeks earlier
she had visited her grandmother, who had shingles. What infection does the 8-year-old have?
A) Chickenpox
B) Measles
C) Fever blisters
D) Scabies
E) Rubella
_____22. The etiologic agent of warts is
A) Papillpmavirus.
B) Poxvirus.
C) Herpesvirus.
D) Parvovirus.
E) Staphylococcus aureus .
_____23. Which of the following is NOT a characteristic of Pseudomonas aeruginosa?
A) Gram-positive cell wall
B) Oxidative metabolism
C) Oxidase-positive
D) Produce pyocyanin
E) Rod shaped
_____24. Which of the following is NOT transmitted by the respiratory route?
A) Smallpox
B) Chickenpox
C) Rubella D) Trichophyton
E) Measles
_____25. Thrush and vaginitis are caused by
A) Herpesvirus.
B) Chlamydia trachomatis.
E) Streptococcus pyogenes.
C) Candida albicans. D) Staphylococcus aureus.
_____26. Which of the following can be treated with topical chemotherapeutic agents?
A) Herpes gladiatorium
B) Sporotrichosis
C) Dermatomycosis
D) Rubella
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E) Shingles
_____27. Which of the following is sensitive to penicillin?
A) Chlamydia
B) Herpesvirus
C) Candida
D) Streptococcus
E) Pseudomonas
_____28. Which of the following is NOT a characteristic used to identify Streptococcus pyogenes?
A) Coagulase-positive
B) Group A cell wall antigen
C) Group M proteins D) Beta-hemolytic
E) Cocci
_____29. Scabies is a skin disease caused by
A) A slow virus.
B) A protozoan.
C) A mite.
D) A bacterium.
E) A prion.
_____30. The patient has vesicles and scabs over her forehead. Microscopic examination of skin scrapings
shows gram-positive cocci in clusters. The etiology is
A) Candida
E) Sarcoptes
B) Microsporum
C) Pseudomonas aeruginosa
D) Staphylococcus aureus
_____31. Which of the following is true about the normal microbiota of the nervous system?
A) Only transient microbiota are present.
B) There are no normal microbiota.
C) Normal microbiota are present in the central nervous system only.
D) Normal microbiota are present in the peripheral nervous system only.
E) Only micrococci are present.
_____32. Encephalitis and meningitis are difficult to treat because
A) They are not caused by bacteria.
B) Antibiotics damage tissues.
C) Antibiotics cannot penetrate the blood–brain barrier.
D) The infections move along peripheral nerves.
E) They are caused by viruses.
_____33. A 15-year-old girl was hospitalized with fever, lethargy, and rash. Gram-negative,
oxidase-positive cocci were cultured from her cerebrospinal fluid. Her symptoms were caused by
A) A prion.
B) Clostridium tetani.
C) Mycobacterium leprae.
D) Neisseria meningitidis.
E) Lyssavirus.
_____34. Which of the following is treated with antibiotics?
A) Botulism
B) Tetanus
C) Streptococcal pneumonia
D) Polio
E) All of the above
_____35. For which of the following is a vaccine NOT available?
A) Haemophilus meningitis
B) Neisseria meningitis
C) Tetanus
_____36. Which of the following is NOT acquired by ingestion?
A) Botulism
B) Cryptococcosis C) Listeriosis D) Poliomyelitis
D) Rabies
E) Botulism
E) Creutzfeldt-Jakob disease
_____37. A 1-year-old boy was listless, irritable, and sleepy. Capsulated gram-negative rods were cultured
from his cerebrospinal fluid. His symptoms were caused by
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A) Neisseria meningitidis.
E) A prion.
B) Rabies.
C) Clostridium tetani.
D) Haemophilus influenzae.
_____38. Which of the following pairs is mismatched?
A) Tetanus – blocks relaxation nerve impulse
B) Botulism – stimulates transmission of nerve impulse
C) Poliomyelitis – kills CNS cells
D) Rabies virus – grows in brain cells
E) All of the above are correctly matched.
_____39. Which of the following vaccines can cause the disease it is designed to prevent?
A) Tetanus toxoid vaccine B) Oral polio vaccine C) Inactivated polio vaccine
D) Haemophilus influenzae capsule vaccine E) Meningococcal capsule vaccine
_____40. Which of the following is NOT caused by prions?
A) Sheep scrapie
B) Kuru
C) Creutzfeldt-Jakob disease
D) Elk chronic wasting disease
E) Rabies
劉世東教授：20%
1. Please describe the structure of a fermentor and the parameters that may affect fermentation.
2. Please describe how Agrobacterium tumefaciens is used to create genetically engineering plants.
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陳怡原副教授：40%
I. Short answers：
1. What molecules are responsible for sepsis caused by Gram-positive and Gram-negative bacterial
infections? (4 pts)
2. (1) What is the causative agent of ‘walking pneumonia’? (a bacterium)
(2) Vancomycin (萬古黴素) cannot eliminate this bacterium, why? (4 pts)
3. Is the reassortment of the RNA segments of the influenza virus the cause of antigenic shift or
antigen drift? (2 pts)
4. Differentiate hepatitis A, hepatitis B, hepatitis C, and hepatitis D. (4 pts)
5. Both genital herpes and genital warts are caused by viruses; which one is more dangerous to a
pregnancy? Why? (4 pts)
6. What are the mode of transmission and symptoms for giardiasis? (4 pts)
7. What are intoxication and infection? (3 pts)
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II.
Matches (each answer may be used more than once) (1 pt per question)
_____1. Scarlet fever
_____2. Lyme disease
_____3. Rheumatic fever
A. Bacillus anthrax
B. Bartonella henselae
C. Bordetella pertussis
_____4. Whooping cough
_____5. Cat-Scratch disease
_____6. Bubonic plaque
_____7. Tularimia
D.
E.
F.
G.
_____8. Burkitt’s lymphoma
_____9. Chagas’ disease
_____10. Gangrene
(Tapeworms)
H. E. coli
I. EB virus
_____11. Syphilis
_____12. Cystitis
_____13. Hydatid disease
J. Francisella sp.
K. Haemophilus ducryi
L. Helicobactor pylori
_____14. Cholera
_____15. peptic ulcer
M. Leishmania sp.
Borelia burgdorferi
Clostridium perfringens
Coxiella burnetii
Echinococcus granulosus
N. Mumps virus
O. Rickettsia sp.
P. Rotavirus
_____16.Q fever
_____17. Tooth decay
_____18. Viral gastroenteritis
_____19. Chancroid
Q.
R.
S.
T.
_____20. α hemolytic streptococci
Staphylococcus aureus
Streptococcus mutans
Streptococcus pyogenes
Toxoplasma gondii
U. Trypanosoma cruzi
V. Tryponema pallidum
W. Vibrio
X. Yersinia pestis
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