* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Cell Membrane - cynthiablairlhs
Cre-Lox recombination wikipedia , lookup
DNA vaccination wikipedia , lookup
Extrachromosomal DNA wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
Polycomb Group Proteins and Cancer wikipedia , lookup
Deoxyribozyme wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
Mir-92 microRNA precursor family wikipedia , lookup
History of genetic engineering wikipedia , lookup
Point mutation wikipedia , lookup
Primary transcript wikipedia , lookup
REVIEW MATCHING) 1. Zoology a. plants 2. Genetics b. animals 3. Anatomy c. heredity 4. Botany d. organisms & their environ- ment 5. Ecology e. structure of organisms 6. Taxonomy f. Change over time 7. Evolution g. Classification MATCH THE FOLLOWING TERMS 8. HOMEOSTASIS 9. METABOLISM 10. INFERENCE 11. THEORY 12. HYPOTHESIS 13. BIOGENESIS A. LIFE COMES FROM OTHER LIFE B. AN INTERPRETATION OF OBSERVATIONS C. A STATEMENT SUMMARIZING MANY WELL SUPPORTED HYPOTHESES D. ALL CHEMICAL REACTIONS IN A CELL E. MAINTAINING STABLE INTERNAL CONDITIONS F. A TESTABLE STATEMENT REVIEW MATCHING 1. 2. 3. 4. 5. 6. 7. 8. 9. A. REGULATES WHAT ENTERS AND LEAVES A CELL NUCLEUS B. “SUICIDE SACS” – DIGESTIVE ENDOPLASMIC STRUCTURE RETICULUM GOLGI COMPLEX C. “POWERHOUSE” – GENERATES ATP MITOCHONDRIA D. “POSTOFFICE” – PACKAGES AND RIBOSOME SHIPS PRODUCTS FROM THE CEL LYSOSOME E. “ROADWAY” – MAKES LIPIDS AND CYTOSKELETON TRANSPORTS SUBSTANCES W/IN CYTOPLASM THE CELL CELL MEMBRANE F. GEL-LIKE FLUID W/IN THE CELL G. “CONTROL CENTER” – HOUSES DNA H. “CELL FACTORY” – SITE OF PROTEIN SYNTHESIS I. MAINTAINS CELL STRUCTURE REVIEW MATCHING 1. OCCURS IN PLANT CELLS 2. OCCURS IN ANIMAL CELLS 3. OCCURS IN THE CHLOROPLAST 4. OCCURS IN THE MITOCHONDRIA 5. 6CO2 + 6 H20 + LIGHT GLUCOSE +6 O2 6. GLUCOSE + 6 O2 6 CO2 + 6 H2O + ATP A. PHOTOSYNTHESIS B. CELLULAR RESPIRATION C. BOTH CELL CYCLE REVIEW 1. Cell division resulting in 2, identical, diploid cells somatic cells 2. Cell division resulting in 4, different haploid cells gametes 3. Time between cell division where cell grows, makes proteins, and prepares to divide 4. Chromatin condense chromosomes; nucleus disappears 5. Chromosomes align in the middle of the cell 6. Chromatids are pulled apart by spindle 7. Chromatids repair with homolog nucleus reforms A. B. C. D. E. F. G. H. METAPHASE PROPHASE MITOSIS ANAPHASE TELOPHASE MEIOSIS CYTOKINESIS INTERPHASE REMEMBER • PRESENTATIONS BEGIN MONDAY • CP BIOLOGY: pg 57 and 59 #s 1-10 due Monday – WRITE LETTER AND ANSWER Biology – The Study of Living things: Many fields 1. Cytology 2. Genetics 3. Evolution 4. Taxonomy 5. Microbiology 6. Zoology 7. Botany 8. Ecology • • • • • Cells Heredity Change over time Classification Microscopic organisms (viruses, bacteria, protists) • Animals • Plants • Interactions of organisms with their environment Characteristics of Living Things 1. Growth 2. Reproduction 3. Respond to Stimuli 4. Genetic Code 5. Metabolism 6. Homeostasis 7. Made of Cells Atoms Molecules Cells Tissues Organs Organ Systems Organism Populations Communities Ecosystems Biomes Biosphere NOT ALL LIVING THINGS CAN BE SEEN TO MOVE • Total energy use (eating, breathing • Maintaining stable internal conditions (body temperature) ORGANIZATION OF LIFE LIVING NON-LIVING = BASIC UNIT OF LIFE Science vs Non-science • Nonscientific • Scientific Statements are Statements falsifiable - can cannot be be disproved disproved (Objective) (Subjective – Opinion based) Flowchart Designing an Experiment Section 1-2 State the Problem Analyze Results Form a Hypothesis Set Up a Controlled Experiment Controls = keep the same Independent/manipulated variable = changed Dependent/responding variable = measured Draw a Conclusion Record Results Qualitative – description Quantitative - numbers Go to Section: Publish Results Theory • A broad statement linking together many WELL SUPPORTED hypotheses – Theory of Evolution – Big Bang Theory – Atomic Theory HOW DOES THE COMMON USE OF THE WORD “THEORY” DIFFER FROM THE SCIENTIFIC DEFINITION? THE TOOLS OF SCIENCE 1. Microscopes a. Compound Light: Uses 2 lenses and light; Can look at living things –Resolution: How clear it is –Magnification: How enlarged it is = Optic * Ocular Lens b. Electron: Uses electrons; Magnification much greater –Organism must be dead 5. SCIENCE TOOLS • MEASURED IN SI (STANDARD INTERNATIONAL UNITS) units. Base units include: – LENGTH = METERS – VOLUME = LITER – MASS = GRAMS • CENTI = 1/100, MILLI = 1/1000, KILO = 1000 • A CENTIMETER = 1/100TH OF A METER GRADUATED CYLINDER TRIPLE BEAM BALANCE BEAKER VOLUME MEASUREMENT (mL) MASS MEASUREMENT (grams) MIXING USE THE PARAGRAPH BELOW TO ANSWER QUESTIONS 1-5. Kellie wants to win the “largest pumpkin” award at the fair. She wants to compare 2 plant foods to see their affect on the pumpkin growth. She designs an experiment to determine the affect of various foods on pumpkin growth. She gives plant food # 1 to pumpkin # 1 and plant food # 2 to pumpkin # 2. She doesn’t give any plant food to pumpkin # 3. All of the pumpkins are kept in the same sunlight, temperature, and given the same amount of water. After 2 weeks, she measures the growth of each pumpkin. 1. What group does Pumpkins # 1 and # 2 represent? (A) Control Group (B) Manipulated Group (C) Data Group (D) Experimental Group (E) Inference 2. What type of data is collected in Kellie’s experiment? (A) qualitative (B) quantitative (C) Is best displayed with a pie graph (D) Represents inductive reasoning 3. The different plant foods represent the: (A) qualitative data (B) control group (C) independent variable (D) dependent variable 4. What represents the control group? (A)Pumpkin # 1 (B) Pumpkin # 2 (C) Pumpkin # 3 (d) The Plant Food 5. The growth of each pumpkin represents the: (A) Control Group (B) Manipulated Variable (C) Responding Variable (D) Inference 6. What would be the best unit to measure the distance from Augusta, GA to Washington D.C.? (A) meters (B) kilometers (C) kilograms (D) seconds (E) cubic centimeters 7. What piece of equipment is best for measuring 20 grams of NaCl? (A) graduated cylinder (B) triple-beam balance (C) Beaker (D) meter stick 8. What piece of equipment is best for measuring 20 ml of water? (A) graduated cylinder (B) triple-beam balance (C) Beaker (D) meter stick 9. The best way to clean a microscope is with: (A) Your sleeve (B) Your hand (C) Special tissues (D) a moistened paper towel 10. You know that a rock is not alive because: (A) It does not reproduce (B) It is not made of cells (C) It does not maintain homeostasis (D) It does not grow (E) All of the above (F) None of the above CHEMISTRY CHEMISTRY – THE STUDY OF THE NATURE, COMPOSITION, AND PROPERTIES OF MATTER • Matter: Anything that has mass (amount of matter) and takes up space - There are 3 States of Matter SOLID LIQUID GAS PARTICLES TIGHTLY VOLUME PACKED VIBRATE FIXED SHAPE FIXED LOOSELY PACKED MOST LOOSELY PACKED FIXED CHANGES CHANGES CHANGES Proton CHARGE + Neutron O Electron - LOCATION Nucleus Nucleus Energy levels DETERMIN Atomic # Atomic mass = proton # if – Atomic # not charged ED BY Or e=pcharge OTHER TERMS ISOTOPE (DIFFERENT NUMBER OF NEUTRONS) ION (CHARGED ATOM) BOND IONIC COVALENT HYDROGEN HOW FORMED Transfer of electrons Sharing of electrons Polarity STRENGTH Medium Strongest Weakest EXAMPLES H2O (water) DNA double helix Na+Cl(sodium chloride – table salt) Cohesion/ Adhesion pH scale: determines acid or base: scale 1-14 • Acid: High concentration of hydronium ions (H+) – Less than 7 on pH scale • Base: High concentration of hydroxide ions (OH-) – Greater than 7 on pH scale – alkaline MACROMOLECULES = ORGANIC COMPOUNDS THAT MAKE UP CELLS IN LIVING THINGS – made of C, H, O, N CARBON IS THE “BACKBONE” OF LIFE ORGANIC COMPOUNDS BUILDING BLOCKS FUNCTIONS EXAMPLES CARBOHYDRATES (SUGARS) MONOSACCH ARIDES PRIMARY/QUICK ENERGY GLUCOSE GLYCOGEN, STARCH LIPIDS (FATS) FATTY ACIDS STORED ENERGY STEROIDS TRIGLYCERIDES PHOSPHOLIPIDS PROTEINS AMINO ACIDS BUILDING AND REPAIRING ENZYMES – SPEED UP RXTS NUCLEIC ACIDS NUCLEOTIDE S CARRY/STORE HEREDITARY INFORMATION DNA RNA CHEMISTRY – Use the paragraph below to answer questions 11-12 After red blood cells pick up carbon dioxide from tissues, the CO2 reacts with water to form carbonic acid. Once in the lungs, it is converted back to CO2 and water, and the CO2 is exhaled. Carbonic anhydrase increases the reaction rate from 200 molecules of carbonic acid to 600,000 carbonic acid molecules per hour 11. Carbonic anhydrase represents: (A) an enzyme (B) a substrate (C) a pigment (D) a reactant 12. What are the building blocks of the substance in # 11? (A) Amino Acids (B) Fatty acids (C) Nucleotides (D) Monosaccharides 13. Covalent bonds result from atoms _______ electrons, while ionic bonds result from _______ electrons. (A) sharing, transferring (B) sharing, sharing (C) transferring, transferring (D) transferring, sharing 14. When a maximum amount of solute is dissolved in a solvent, the solution is: (A) Saturated (B) Covalently bonded (C) Distilled (D) Separated 15. Which of the following represents a primary energy source for cells? (A) Carbohydrates (B) Proteins (C) Nucleic Acids (D) Lipids 16. Cohesion and adhesion are both possible due to which water property? (A) Polarity (B) Solubility (C) Transparency (D) Ionic bonding 17. If an atom has an atomic number of 8 and has a charge of -2, how many electrons does it have? (A) 6 (B) 8 (C) 10 (D) 0 18. Stomach acid would be expected to have a pH around: (A) 2 (B) 7 (C) 9 (D) 13 19. The primary solute in the cytoplasm of a cell is:(A) Water (B) NaCl (C) Lipids (D) Nucleic Acids 20. Carbon 14 has 2 more neutrons than Carbon 12. Carbon 14 represents a: (A) ion (B) isotope (C) compound (D) organic compound CELL PARTS PLANT & ANIMAL CELLS HISTORY OF CELL DISCOVERIES -1665 – ROBERT HOOKE – First discovered and named cells (in non-living cork) -1673 – Anton Van Leeuwenhoek - “Father of MICROSCOPY”. – *First to describe LIVING CELLS AND MICROSCOPIC STRUCTURES The CELL THEORY: 1800s 1. ALL LIVING THINGS ARE MADE OF CELLS. (Schleiden and Schwann) 2. CELLS ARE THE BASIC UNIT OF LIFE 3. CELLS COME ONLY FROM OTHER CELLS (Virchow) 2 KINDS OF CELLS: PROKARYOTES & EUKARYOTES PROKARYOTE = NO NUCLEUS OR MEMBRANEBOUND ORGANELLES EX. = BACTERIA EUKARYOTE – HAS A NUCLEUS & MEMBRANE-BOUND ORGANELLES EX. = PLANTS, ANIMALS REVIEW OF CELL PARTS How do plant and animal cells differ? CLICK ON THIS FOR A REVIEW OF THE FUNCTIONS: http://sps.k12.ar.us/massengale/cell_functions.htm ORGANELLE FUNCTION NUCLEUS CONTROLS Cell Activities; Contains genetic information ENDOPLASMIC RETICULUM (ER) ROUGH ER – With ribosomes; Produce products for export SMOOTH ER – No ribosomes; System of channels RIBOSOMES Site of PROTEIN synthesis; Can be attached to ER or free in cytoplasm GOLGI BODIES Responsible for PACKAGING and TRANSPORT OF cell products MITOCHONDRIA Site of ENERGY production (ATP) LYSOSOMES Site of DIGESTION, DISPOSAL, and LYSIS of cells PEROXISOMES Site of FILTERING toxic materials CYTOSKELETON Involved in support, transport, and reproduction of cell VACUOLES STORAGE of extra sugar and water PLANTS/ALGAE ONLY CHLOROPLASTS CONVERT SOLAR ENERGY AND CO2 GLUCOSE AND O2 • CELL MEMBRANE (“LIPID BILAYER”) – MADE OF LIPIDS AND PROTEINS – SELECTIVELY PERMEABLE • The Cell WALL – Tough, outer layer outside of cell membrane IN BACTERIA, ALGAE, AND FUNGI DIFFUSION = MOVEMENT OF MOLECULES FROM HIGH CONCENTRATION (MORE MOLECULES) TO LOW CONCENTRATION (LESS MOLECULES) = DIFFUSION OF H 20 EFFECTS OF SOLUTIONS ON CELLS – HOW CELLS MAINTAIN HOMEOSTASIS PURE WATER SALT WATER EXPLAIN WHAT IS HAPPENING ACTIVE TRANSPORT = movement of molecules from LOW to HIGH concentration HIGH LOW REQUIRES ENERGY EOCT DAY 2 QUIZ • NUMBER FROM 1-15 1. The organelle that is the site of protein synthesis is: (A) Ribosome (B) Golgi Body (C) Nucleus (D) Cell Membrane 2. If a cell is placed in a solution with a high concentration of glucose, water will: (A) Enter the cell by osmosis (B) Exit the cell by osmosis (C) Exit the cell by active transport (D) Enter the cell by facilitated diffusion 3. What will happen to an animal cell if it is left in a solution of distilled water overnight? (A) It will shrivel (B) It will swell and burst (C) It will remain at equilibrium (D) It will undergo photosynthesis 4. The macromolecules which have the greatest variety of function and are the main component of Cell Membranes are:(A) Lipids (B) Carbohydrates (C) Nucleic Acids (D) Proteins 5. The individual who discovered and named cells was: (A) Anton Van Leeuenhoek (B) Charles Darwin (C) Robert Hooke (D) Gregor Mendel 6. An egg that was left in a solution overnight was shriveled the next day. The solution was: (A) Hypertonic (B) Hypotonic (C) Isotonic (D) Salty 7. The organelle in which DNA is stored is the: (A) Mitochondria (B) Nucleus (C) Ribosome (D) Cell Membrane 8. What best describes the Cell Theory? (A) All living things are made of cells (B) Cells come from pre-existing cells (C) Cells are the basic unit of all life (D) All of the above (E) None of the above 9. Eukaryotic cells differ from prokaryotic cells in that (A) Eukaryotic cells are living and prokaryotic cells are not (B) Eukaryotic cells have a true nucleus and membrane bound organelles (C) Eukaryotic cells are much smaller than prokaryotic cells CELL ENERGY ATP = ENERGY STORAGE COMPOUND IN CELLS LIKE AN UNCHARGED BATTERY LIKE A CHARGED BATTERY WHY DO PHOTOSYNTHESIS & RESPIRATION DEPEND ON EACH OTHER? GLUCOSE + ATP WHICH IS PHOTOSYNTHESIS & WHICH IS CELL RESPIRATION? C6H1206 + O2 6CO2 + 6H2O + 38 ATP In mitochondria PLANTS & ANIMALS DO THIS TO RELEASE ENERGY 6CO2 + 6H2O + LIGHT C6H1206 + O2 In chloroplasts ONLY PLANTS DO THIS TO STORE ENERGY CELL REPRODUCTION STAGES OF MITOSIS – cell division to make organisms grow & repair themselves INTERPHASE = PERIOD BETWEEN CELL DIVISIONS PMAT = PROPHASE METAPHASE ANAPHASE TELOPHASE CYTOKINESIS = DIVISION OF CYTOPLASM MITOSIS – makes 2 identical cells with same DNA – happens in all forms of ASEXUAL REPRODUCTION PARENT CELL WITH 46 CHROMOSOMES 46 46 46 DAUGHTER CELLS HAVE 46 CHROMOSOMES MEIOSIS = cell division in ovaries & testes to produce gametes (eggs & sperm) 23 23 23 46 23 23 23 Human ovary or testis cell starts with 46 chromosomes. After meiosis, the eggs & sperm only have 23 chromosomes. SEXUAL REPRODUCTION – involves 2 sex cells (gametes) offspring will be a combination of the 2 parents HAPLOID HAPLOID + EGG (23) ZYGOTE (46) DIPLOID SPERM (23) Comparing Mitosis and Meiosis • Mitosis –2 cells formed –Cells are diploid (full set of chromosomes) –Cells are identical to “parent” cell –Forms all cells besides sex cells • Meiosis –4 cells formed –Cells are haploid – (half set of chromosomes) –Cells vary from “parent” cells –Forms sex cells (gametes) 10. Which of the statements is NOT true concerning the following reactions? 1. 6CO2+ 6 H2O + light energy Glucose + 6 O2 2. Glucose + 6 O2 6 H2O + energy (A) Chemical energy is converted to cellular energy in photosynthesis, and light energy is changed into chemical energy in respiration (B) Photosynthesis requires energy while respiration releases energy (C) Respiration is an ecothermic reaction while photosynthesis is endothermic (D) Oxygen is a reactant in respiration and product of photosynthesis (E) The 1st formula represents photosynthesis while the 2nd represents respiration. 11. Cellular respiration is to __________ as photosynthesis is to __________. (A) Mitochondria, Photosynthesis (B) Photosynthesis, Mitochondria (C) Nucleus, Cell Membrane (D) Golgi bodies, Endoplasmic reticulum 12. Put the following stages of Mitosis in order: (A) Prophase, Metaphase, Telophase, Anaphase (B) Metaphase, Prophase, Anaphase, Telophase (C) Prophase, Metaphase, Anaphase, Telophase (D) Telophase, Anaphase, Metaphase, Prophase 13. What best describes the difference between mitosis and meiosis? (A) Mitosis produces 2 identical and diploid cells, and meiosis produces 4 different and haploid cells (B) Mitosis produces 4 different and haploid cells, and meiosis produces 2 identical and diploid cells (C) Mitosis only produces gametes (D) Meiosis is used in the repair of body cells 14. Cell Division differs in a plant cell from an animal cell at which step? (A) Prophase (B) Metaphase (C) Anaphase (D) Telophase (E) Cytokinesis DNA, RNA & PROTEIN SYNTHESIS WHAT IS DNA? • Blueprint of Life (has the instructions for making an organism) GENE = a segment of DNA that codes for a protein, which codes for a trait (skin color, eye color, etc.) DNA is wrapped around protein to form chromosome • Structure was discovered by James Watson and Francis Crick (1953) • Described shape as a double helix (“twisted ladder”) DNA STRUCTURE • Made of repeating subunits called NUCLEOTIDES = sugar, phosphate, nitrogen base • DEOXYRIBOSE SUGAR and PHOSPHATES (sides of ladder) • NITROGEN BASES (rungs of ladder) ADENINE (A) GUANINE (G) THYMINE (T) CYTOSINE (C) • Attach to deoxyribose sugar • Always follow “base-pair rule” • Pair up A - T , C - G • Connected by weak HYDROGEN BONDS DNA REPLICATION – DNA makes a copy of itself • Occurs during interphase before MITOSIS, so the new cells will be identical to the parent cell DNA, RNA, & PROTEIN SYNTHESIS DNA FUNCTION CARRIES HEREDITARY INFO GENES = PORTIONS OF DNA PROTEIN TRAIT CHROMOSOME = DNA WRAPPED AROUND PROTEIN (46 IN US) WHERE FOUND NUCLEUS OF CELL SHAPE DOUBLE HELIX – (TWISTED LADDER) -DISCOVERED BY WATSON AND CRICK (1953) STRUCTURE -DOUBLE STRANDED NUCLEOTIDES = SUGAR (DEOXYRIBOSE, PHOSPHATE, AND BASE (A, T, C, G); PAIR UP A –T; G – C PROCESS REPLICATION: DNA MAKES AN IDENTICAL COPY OF ITSELF (SEMICONSERVATIVE) -OCCURS IN S PHASE (INTERPHASE) OF CELL CYCLE DNA is too big to leave the nucleus so RNA (Ribonucleic Acid) must get involved. SIMILARITIES IN DNA & RNA Both have N bases adenine, guanine, cytosine DIFFERENCES DNA • Deoxyribose sugar • Thymine • Double-stranded RNA • Ribose sugar • Uracil • Single-stranded TYPES OF RNA mRNA rRNA tRNA Messenger RNA Ribosomal RNA Transfer RNA Copies DNA code & takes it to ribosome Makes up ribosome Picks up amino acids in cytoplasm & brings them to ribosome HOW DOES mRNA COPY THE DNA CODE? DNA - TAC GGC TAA ACT mRNA – AUG CCG AUU UGA REMEMBER…RNA has uracil instead of thymine to bond with adenine! RNA FUNCTION CARRIES HEREDITARY INFO FROM DNA IN NUCLEUS TO RIBOSOME PROTEIN WHERE FOUND NUCLEUS AND RIBOSOME STRUCTURE -SINGLE STRANDED -NUCLEOTIDES = SUGAR (RIBOSE, PHOSPHATE, AND BASE (A, T, C, G); PAIR UP -A, U, G, C PROCESS TRANSCRIPTION – FORMATION OF RNA FROM DNA (IN NUCLEUS) TRANSLATION – FORMATION OF A PROTEIN FROM RNA (ON A RIBOSOME) -CODON = SET OF 3 mRNA bases that code for an amino acid AMINO ACIDS • 20 exist • Link together to make proteins during protein synthesis • TRIPLET CODON = 3 NITROGEN BASES 1 AMINO ACID mRNA codon chart PROTEIN SYNTHESIS is the making of a protein. To make a protein, a cell needs: • • • • DNA - holds the code in the nucleus mRNA codons - carries code to ribosome RIBOSOME - where protein is made tRNA anticodon - picks up amino acids & brings them to ribosome • AMINO ACIDS - building blocks of proteins Step #1 - TRANSCRIPTION • Making of mRNA in the nucleus to copy the DNA code to take to the ribosome Step #2 - TRANSLATION • tRNA anticodon picks up the correct amino acid in the cytoplasm and takes it to the ribosome to make the protein PROTEIN SYNTHESIS 1. TRANSCRIPTION RIBOSOME NUCLEUS DNA mRNA CODONS 2. TRANSLATION mRNA tRNA codons anticodons matched up at ribosome AMINO ACIDS PROTEIN CHANGE IN THE DNA CODE MUTATIONS Can be passed to offspring if it occurs in sex cells Some may be beneficial evolution of species Caused by MUTAGENS RADIATION ENVIRONMENTAL POLLUTANTS DRUGS/CHEMICALS TYPES CHROMOSOME POINT (GENE) AFFECTS # OR ENTIRE CHROMOSOME FRAMESHIFT INSERTION SUBSTITUTION DELETION QUIZ DAY 3 – DNA, GENETICS AND EVOLUTION 1. What molecule carries the DNA information from the nucleus to the ribosome for translation? (A) tRNA (B) mRNA (C)rRNA (D) ATP 2. A strand of DNA with the base pair sequence AATGGCCATT would have the complimentary strand: (A) AATGGCCATT (B) UUACCGGUAA (C) TTACGCGTTA (D) TTACCGGTAA 3. DNA contains the information needed to make: (A) proteins (B) Glucose (C) Fatty Acids (D) ATP 4. A strand of DNA with the base pair sequence AATGGCCATT would produce an RNA strand: (A) AATGGCCATT (B) UUACCGGUAA (C) TTACGCGTTA (D) TTACCGGTAA 5. What best describes a codon? (A) a set of 3 DNA bases that codes for RNA (B) a set of 3 mRNA bases that codes for an amino acid (C) a set of 3 tRNA bases that codes for an amino acid (D) a set of 3 rRNA bases that codes for a ribosome 6. Which of the following is NOT a difference between DNA and RNA? (A) DNA is double stranded and RNA is single stranded (B) DNA is formed in the nucleus while RNA is not (C) RNA contains the base Uracil instead of Thymine (D) DNA contains the sugar deoxyribose and RNA contains ribose 7. DNA produces more DNA in a process called ______________. DNA is copied to RNA in a process called ______________. RNA codes for a protein in a process called _______________. (A) Transcription, Translation, Replication (B) Replication, Transcription, Translation (C) Replication, Translation, Transcription (D) Transcription, Replication, Translation GENETICS IV. MENDEL’S EXPERIMENTS A. Self-pollinated plants (true-breeding) Purple X purple All Purple White X white All White B. Cross – pollinated plants w/ contrasting traits (P) Purple X white* All Purple (F1) Tall X short* All Tall * Trait seemed to disappear in the 2nd, or F1 generation 1 trait always disappeared in the 2nd, or F1 generation Round/ wrinkled Yellow/ green Gray/ white Inflated/ constricted Yellow pod/ Green pod Axial/ terminal Tall/short IV. MENDEL’S EXPERIMENTS cont. C. Self-pollinated F1 plants F2 generation • Purple (F1) X purple (F1) ¾ Purple, ¼ white (F2) • Tall X tall ¾ Tall, ¼ Short • Missing traits reappeared The trait reappeared in the 3rd, or F2 generation The white trait “skipped” a generation! VI. MENDEL’S CONCLUSIONS 1. Traits are controlled by genes, that occur in pairs. 2. LAW OF DOMINANCE • Some traits hide the effect of others. • Allele – a single gene – 2 alleles Trait – Alleles may be: • Dominant – Hides the other trait • Recessive – Trait seems to disappear –Ex. Tall x short = All tall OTHER GENETIC TERMS • HOMOZYGOUS = pure = organism w/ same alleles for a trait (TT,tt) • HETEROZYGOUS = hybrid = different alleles for a trait (Tt) •GENOTYPE – Alleles (genes) of a trait (letters) •PHENOTYPE – Physical appearance (words) Homozygous dominant - TT Heterozygous – Tt Homozygous recessive - tt Tall plant Tall plant Short plant HOW CAN WE USE MATH TO FIGURE ALL THIS OUT? • PROBABILITY - chance that something will or will not happen 1 out of 2 or 50 % that a flipped coin will land on heads Not dependent on previous result; You could flip heads 10 straight times • PUNNETT SQUARE – chart used to predict probable outcomes in a genetic cross HOW TO SET UP A PUNNETT SQUARE TALL X SHORT TT X tt HETEROZYGOUS TALL X HETEROZYGOUS TALL Tt x Tt MONOHYBRID CROSS • involves 1 pair of alleles 1 trait • FLOWER COLOR TRY THIS ONE GIVE THE GENOTYPIC RATIO & PHENOTYPIC RATIO Tongue rolling (T) is dominant to nontongue rolling (t) What is the probability of tongue rollers for the following crosses? 1) Homozygous dominant x Homozygous recessive 2) Heterozygous x homozygous recessive 3) Neither parent can roll their tongue WHAT IF THE TRAIT IS NEITHER DOMINANT NOR RECESSIVE? INCOMPLETE DOMINANCE • Neither gene is dominant • Heterozygous condition produces “mixture” of traits” (blend) • Ex. Snapdragons, 4 o’clocks • PHENOTYPE • GENOTYPE Red X white Pink RR X WW RW WORK THIS PUNNETT SQUARE. SEX-LINKED TRAITS • Due to genes on X chromosome – XX = FEMALE; XY = Male – SEX-LINKED Diseases are more common in males they lack a backup chromosome • Muscular Dystrophy, Colorblindness, and Hemophilia • The other 22 pair are called AUTOSOMES 22 PAIRS OF AUTOSOMES 1 PAIR SEX CHROMOSOMES Practice: Colorblindness is a sexlinked recessive trait A) What is the probability that a female will be colorblind if: • Her father is colorblind and • Her mother is a carrier? B) Probability of a male? GENETIC ENGINEERING Taking DNA from 1 organism and putting it into another organism EX. Human gene for insulin was put into bacteria so bacteria can make human insulin for diabetics 9. A gene mutation in gene p53 can cause skin cancer. This mutation is usually caused by too much sun exposure. How could one mother who had a mutation in gene pg53 and skin cancer and a mother without the mutation or skin cancer both had children without the mutation or skin cancer? (A) Neither father had the skin cell mutation, and the disease is a homozygous recessive one (B) The mother without the mutation did not sunbathe during pregnancy (C) Neither child came down with the illness because only mutations in gamete genes can be passed on (D) It is random chance that neither child came down with the condition. 10. If one parent was homozygous dominant for a trait and the other was heterozygous, what are the expected genotypes of the offspring. (A) 25 % homozygous dominant, 50 % heterozygous, 25 % homozygous recessive (B) 100% homozygous dominant (C) 50 % homozygous dominant, 50 % heterozygous (D) 50 % homozygous dominant, 50 % homozygous recessive. 11. If one allele masks another one, it is said to be: (A) Recessive (B) Dominant (C) Homozygous (D) Heterozygous 12. A mother is homozygous recessive for not being able to roll her tongue. The father is heterozygous and can roll his tongue. What is the chance that the child will be homozygous recessive and not be able to roll his/her tongue? (A) 0% (B) 25 % (C) 50 % (D) 75 % (E) 100% 13. The disease that results from non-disjunction in the 21st pair of chromosomes, leading to a 3rd 21st chromosome is called: (A) Down Syndrome (B) Hemophilia (C) Muscular Dystrophy (D) Cystic fibrosis 14. The “father of heredity” is: (A) Charles Darwin (B) Gregor Mendel (C) Robert Hooke (D) Carl Linnaeus EVOLUTION • Evolution is considered the Unifying Theory in Biology It uses all fields of Biology to provide a possible answer to these difficult questions Radioactive dating – Use isotopes to date ancient materials Half-life: how long it takes for half to decay –Carbon Dating: Dates 1000s of yrs –Other Elements used to date millions of yrs I. Theorized History of Earth • • • • • 14 Billion Years: Big Bang 5 Billion Years: Beginning of Solar System 4.5 Billion Years: Origin of Earth 3.5 Billion Years: Prokaryotes dominate 2.5 Billion Years: Oxygen Accumulates in Atmosphere • 1.5 Billion Years: Eukaryotes Appear • 0.5 Billion Years: Cambrian Explosion of multicellular organisms II. Theories for life origin A. Miller and Urey – Tested to see if organic compounds could spontaneously form without life B. Prokaryotes theoretically came first Released oxygen formed ozone layer absorbs UV light – Allowed organisms to move to land C. Endosymbiosis: Eukaryotes came from prokaryotes: - Bacteria Mitochondria and Chloroplasts - Mutualism B. History of Evolutionary Theory • Lamarck (1801) – Said acquired traits were passed to offspring; easily disproved –The offspring of flies with clipped wings have normal length wings –If you lost a limb in an accident, your kids wouldn’t have 1 less limb History of evolution cont. 2. Charles Darwin - “Father of evolution” (1809-1882) a. Gathered data at The Galapagos Islands b. “Darwin’s finches” – 13 different finch species • Specialized beaks for feeding • Came from 1 finch ancestor c. 1858 - Published On the Origin of Species C. How Evolution Happens • 1) Overproduction • 2) Variation - through mutation, migration, sexual reproduction • 3) Natural Selection a. The environment “favors” certain variations (“Survival of the fittest”) b. Organisms with favorable traits are more likely to survive and reproduce – These organisms have a high “fitness” • 4) Isolation TYPES OF EVOLUTION CONVERGENT DIVERGENT SIMILAR TRAITS DEVELOPED INDEPENDENTLY SIMILAR TRAITS DUE TO COMMON ANCESTRY EVIDENCES OF EVOLUTION 1. HOMOLOGOUS STRUCTURES 2. VESTIGIAL STRUCTURES – “LEFTOVERS” (APPENDIX) 3. EMBRYOLOGY – SIMILAR DVPT 4. FOSSILS – “MISSING LINKS” 5. DNA COMPARISONS – SIMILAR DNA INDICATES MORE RECENT ANCESTRY V. A Cladogram represents a “tree of life” that shows the theorized relationship between organisms TAXONOMY The study of classifying & naming organisms based on evolutionary relationships CAROLUS LINNAEUS (1707-1778) • Developed modern, universal classification of organisms (17071778) Binomial nomenclature: (2-name naming system) by Genus and species • Capitalize Genus and lower case species • Underline or Italicize Ex. Homo sapiens Canis familiaris Felis domesticus CLASSIFICATION GROUPINGS – from broad to narrow based on evolutionary relationships • KINGDOM divided into PHYLUM (phyla) divided into CLASS divided into ORDER divided into FAMILY divided into GENUS (genera) divided into SPECIES 6 Kingdoms 1) Archaebacteria: Ancient bacteria Ex. methanogens 2) Eubacteria: “True” bacteria Ex. E. coli, Salmonella 3) Protista: “Dumping ground” Ex. Protozoa, algae Prokaryotes; Unicellular; Different Cell Wall than Eubacteria Can withstand extreme conditions Prokaryotes; Unicellular; Most numerous on earth -Eukaryotes; Mostly unicellular – Both autotrophic and heterotrophic members 6 Kingdoms Continued 4) Fungi Ex. Mushroom, mold 5) Plantae: Ex. Oak tree, tomato 6) Animalia: Ex. Human, crayfish, earthworm Eukaryotes; Multicellular Cell Wall; Heterotrophic Eukaryotes; Multicellular Cell Wall; Autotrophic Eukaryotes; Multicellular; No Cell Wall; Heterotrophic Example: Use a DICHOTOMOUS Key to Identify a Snake, Frog, Fish, and Cat 1a. Has hair……………….. 1b. Has no hair…………… …Cat 2a. Has Legs……………….. 2b. Has no legs…………….. …….Frog 3a. Has gills……………. 3b. Has no gills……….. ……….Fish ………. Snake …go to 2 …… go to 3 • MICROBIOLOGY Characteristics OF VIRUSES • Very Small (Smaller than bacteria) – Only seen with electron microscope – COMPARE IT: http://www.cellsalive.com/howbig.htm • Subcellular – No cell parts; Made of: 1. 2. Nucleic Acid: DNA or RNA Capsid – Protein coat around nucleic acid a. Envelope – Surrounds capsid b. Protein “spikes” - help them attach to cells • Living or Non-living? • No life activities except reproduction - Requires a host (a living cell) to do this Reproduction – Requires a host Lytic Cycle – Causes immediate disease (i.e. flu) – 5 steps Lysogenic cycle Virus is dormant before causing disease (i.e. HIV) • Viral DNA becomes 1) Attachment part of cell’s DNA 2) Entry (by injection, hides endocytosis, or injured • Enters into Lytic cell wall/membrane) cycle after infecting 3) Replication 1000s of cells 4) Assembly 5) Rupture (Lysis) LYTIC CYCLE LYSOGENIC CYCLE Humans and Viruses cont. Viral Diseases: cold, HIV, chickenpox, measles, mumps, polio, rabies, hepatitis, smallpox, influenza, … 1) Treatments/Prevention – Antiviral drugs – Interfere with viral replication – Vaccines – dead or attenuated viruses that stimulate immune system • Created by EDWARD JENNER – Antibiotics are NOT effective 2) Control carriers (rabies shots, mosquito control) 3) These have led to eradication of some viruses (smallpox, polio) BACTERIA (Kingdom Monera) all prokaryotic and unicellular DIVIDED INTO • Archaebacteria (“Ancient”) • EXTREMOPHILES 2 Kingdoms • Eubacteria (“True”) • COMMON BACT. Kingdom Eubacteria: Structure – 1. Parts a. Plasmid (circular DNA), ribosomes, and cytoplasm b. Cell Membrane and Cell Wall – Gram stain indicates type (+ or -) c. Capsule – for protection i. Pili – to attach to cell ii. Cilia or Flagella – For movement iii. Endospore – dormant, protective structure to survive harsh conditions Bacterial diseases can be cured by Antibiotics • Discovered (Penicillin) in 1918 by Alexander Fleming • Different types based on gram stain (+ or -) • Antibiotic resistance results from overuse or misuse KINGDOM PROTISTA ARE CLASSIFIED AS ANIMAL-LIKE PROTOZOA PLANT-LIKE Phytoplankton ( Algae) FUNGUS-LIKE SLIME MOLDS/ Water Molds PROTOZOA (“Animal-like Protists”) Compose ZOOPLANKTON (big part of food chains in water environments Classified by MOVEMENT into 4 phyla Type MOVEMENT EXAMPLE FLAGELLATES flagella Dinoflagellates ( red tide) Pseudopoda pseudopod Ameoba ciliates cilia Paramecium Sporozoa none Plasmodium malaria Trypanosoma-> African Sleeping Sickness Ameoba proteus Paramecia pictures ALGAE – Plant-like Protists • All have chlorophyll • All make food by carrying out photosynthesis (autotrophic) • Classified by Color & structure DINOFLAGELLATES Red tide GREEN Euglena, Spirogyra, Desmids GOLD Diatoms RED Agar BROWN Kelp, Rockweed IMPORTANCE OF ALGAE • Food & oxygen source for lots of organisms • ALGIN – thicken soups & salad dressing • CARRAGEENAN – smooth ice cream • AGAR – grow bacteria • DIATOMACEOUS EARTH – toothpaste, cleansers, road paint FUNGI CLASSIFIED INTO 4 PHYLA based on structure & method of reproduction IMPERFECT FUNGI (DEUTEROMYCOTA) COMMON MOLDS (ZYGOMYCOTA) RHIZOPUS CLUB FUNGI (BASIDIOMYCOTA MUSHROOMS, PUFFBALLS) SAC FUNGI (ASCOMYCOTA) Morels Truffles PENICILLIUM ATHLETE’S FOOT NO SEXUAL REPRODUCTION INVERTEBRATE COMPARISON EXAMPLE SYMMETRY DIGESTION CIRCULATIO N EXCRETION RESPIRATIO N PORIFERA SPONGES NONE FILTER FEEDER NONE DIFFUSION DIFFUSION CNIDARIANS HYDRA, JELLYFISH, CORAL RADIAL GASTROVAS CULAR CAVITY NONE DIFFUSION “ PLATYHELMI NTHES (FLAT WORMS) PLANARIA, TAPEWORM, FLUKE BILATERAL MOUTH, PHARYNX, INTESTINE (1 OPENING) NONE FLAME CELLS “ NEMATODA (ROUND WORMS) ASCARIS, HOOKWORM , TRICHINA BILATERAL 2 OPENING NONE FLAME CELLS “ ANNELIDA (SEGMENTE D WORMS) LEECH, EARTHWOR M BILATERAL 2 OPENING CROP, GIZZARD CLOSED W/ AORTIC ARCHES NEPHRIDIA “ OPEN (CLOSED IN OCTUPUS) NEPHRIDIA GILLS OPEN MALPIGHIAN TUBULE GREEN GLAND SPIRACLES AND TRACHEAL TUBES GILLS MOLLUSKA (SOFT BODY) ARTHROPO DA BILATERAL SPIDER, INSECT, CRAYFISH BILATERAL 2 OPENING, CROP/GIZZA RD VERTEBRATE COMPARISON FISH AMPHIBIANS REPTILES BIRDS MAMMALS EXAMPLES LAMPREY, SHARK, TROUT FROG, TOAD, SALAMANDER SNAKE, LIZARD, TURTLE ALLIGATOR ROBIN, CROW, CHICKEN HUMAN, CAT, WHALE CHARACTERIS TICS SCALES, GILLS, FINS LEGS, LUNGS, THIN SKIN AMNIOTE EGG, SCALY SKIN FEATJERS HAIR, MAMMARY BODY TEMP ECTOTHERMIC ECTOTHERMIC ECTOTHERMIC ENDOTHERMIC ENDOTHERMIC HABITAT WATER WATER LAND LAND LAND LAND # OF HEART CHAMBERS 2 3 3 ( 4 in Crocodilians) 4 4 CIRCULATION SINGLE-LOOP DOUBLE – LOOP DOUBLE – LOOP DOUBLE – LOOP DOUBLE – LOOP RESPIRATION GILLS GILLS LUNGS LUNGS LUNGS W/ AIR SACS LUNGS EXCRETION KIDNEYS KIDNEYS KIDNEYS KIDNEYS KIDNEYS REPRODUCTIO N EXTERNAL OVIPAROUS EXTERNAL OVIPAROUS INTERNAL OVIPAROUS INTERNAL OVIPAROUS INTERNAL VIVIPAROUS OTHER SWIM BLADDER, LATERAL LINE, OPERCULUM NICTITATING MEMBRANE TYMPANUM CLOACA JACOBSON’S ORGAN, HEMOTOXIN, NEUROTOXIN ARCHEOPTER YX MONOTREME MARSUPIAL PLACENTAL GLANDS ORGANIZATION OF THE BIOSPHERE Organism Populations Bass in pond Same Species in area Communities all living things in an area Bass + duckweed + Turtles + algae in pond Ecosystems Biotic + Abiotic Factors Sunlight,H2O, Fish, plants Biomes Ecosystems with same climate Biosphere All of Earth’s Biomes Desert, ocean, rainforest SUCCESSION IN A POND ECOSYSTEM -BRIEFLY DESCRIBE THE PROCESS IS OCCURING -ORGANIZE THE PICTURES FROM THE 1ST TO LAST STEP IN THE PROCESS -BRIEFLY EXPLAIN WHAT IS HAPPENING IN THE DIAGRAM BELOW. -WHAT IS THE HARDWOOD FOREST CALLED? Succession - The changing of an ecosystem over time due to natural processes DEFINITION EXAMPLE Primary Succession Occurs where there is no soil or life Rock, volcanic islands; parking lots and sidewalks Secondary Succession Occurs in an area that has been disturbed and is returning to its natural community CLIMAX COMMUNITY A mature, stable TROPICAL RAIN ecosystem that will change FOREST very little contains a large amount of biodiversity (different species of organisms) fields becoming forests BIOME TEMPER ATURE RAINFALL ORGANISMS LOCATION TROPICAL RAINFOREST WARM HIGH HIGHEST BIODIVERSITY -TREE DWELLING ANIMALS NEAR EQUATOR TEMPERATE DECIDUOUS FOREST moderate moderate Trees that lose leaves, deer, bears, snakes SOUTHEAST U.S. (WHERE WE LIVE) TAIGA COLD MODERATEHIGH SNOWFALL conifers and furry animals SAVANNA LOW Zebras, giraffes, lions, small plants, a few trees African equator HIGH TUNDRA VERY COLD LOW Polar bears, arctic foxes, seals, etc POLAR REGIONS (Alaska and Canada) PERMAFROST (frozen soil) PREVENTS GROWTH OF BIG TREES DESERT HOT TEMPERATE GRASSLAND LOW LOW RAINSHADOW Tall grasses, buffalo, prairie dogs CACTI, BOBCAT, OWLS, LIZARDS & SNAKES NORTHERN AFRICA (SAHARA) MID-WEST U.S. LOW WHAT IS A BIOME? BIOME CATEGORIES –Marine – saltwater; oceans –ESTUARY – BRACKISH WATER –Freshwater – rivers, lakes, streams –Forests – enough rain for trees –Grasslands – not enough rain for trees, mainly small plants Species Relationships • Predation (+/-) (1 chases & eats the other) • Parasitism (+/-) (1 lives on the other) • Commensalism (+/0) – 1 benefits, other not affected • Mutualism (+/+) (both benefit) • Competition (-/-) (Both fight for same resources) Example of a Food Chain Quaternary consumer Eagle Tertiary consumer Snake Secondary consumer Primary consumer Producer Frog Grasshoppers Grass D e c o m p o s e r s Identify the Producers, Consumers, & Decomposers: Count the Food Chains! 4TH TROPHIC LEVEL 3RD TROPHIC LEVEL 2ND TROPHIC LEVEL 1ST TROPHIC LEVEL B. Energy Flow Pyramid • With each transfer, some energy is lost to heat. -Only 10% of the energy is transferred to the next trophic level 1 person 10 trout 100 dragonflies 1000 insect larvae 10,000 phytoplankton CARBON CYCLE