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Name:_____________________________________Date:______________________Per:_________ Enzyme Web Quest Directions: Please go to the following website and answer the questions based of the information presented about enzymes. http://leavingbio.net/ENZYMES.htm Periodically you will need to hit the reload arrow to see the animations. Read the first paragraph and fill in the blanks below: A ____________ is a substance that ___________ up a chemical (metabolic) reaction. The catalyst itself is not used up as a result of its actions. Proteins that function as biological catalysts are called ________________. They are composed of C, H, O and N. Sulphur (S) may also be present As we saw in the nutrition and food webpage the function of proteins is determined by their ___________ __________________ composition as well as their shape. Enzymes control cellular reactions. As you remember, reactions that break down substances and release energy are called catabolic reactions. Examples are __________ and _____________. The other types of reactions are called anabolic reactions. These reactions consume (_________) energy. These reactions build larger, more complex, molecules from smaller ones. ________________ and ____________ growth from amino acids are examples of anabolic reactions. Enzyme Action: Read an answer the questions from the enzyme action section. 1. What shapes are enzymes folded into? 2. What is special about this shape? 3. Explain the “active site.” 4. What is the substrate? Draw diagram à 5. How does enzyme action occur? 6. What is an enzyme-substrate complex? 7. Can the enzyme be used again after this? Explain. 8. Differentiate between anabolic and catabolic: 9. Now give an example of a catabolic enzyme: 10. Give an example of an anabolic enzyme: Types of Enzyme Action: Read, review and answer J 1. Explain the “lock and key model” of enzymatic action. 2. What does enzyme specificity mean? 3. What is an “induced fit model”? Make sure you reload the page to view the animation. Names of Enzymes: Complete the following: Enzymes are names by their ______________. The letters _____ are added to the substrates name. Match the following: __ breaks down lactose (milk sugars) __ digests complex sugars and starches __ breaks down proteins found in meats, nuts, eggs, and cheese __ breaks down cellulose, plant fiber; not found in humans __ digests vegetable starch __ digests disaccharides to monosaccharides (malt sugars) __ breaks down starch to glucose __ breaks down fats found in most dairy products, nuts, oils, and meat A. DIASTASE B. GLUCOAMYLASE C. LACATASE D. CELLULASE E. MALTASE F. PROTEASE G. LIPASE H. SUCRASE General Effects of Enzymes: 1. 2. 3. 4. Enzymes _________ activation energy Regulates thousands of different ________ reactions in a cell. Activity is determined by which _________ are active at that moment. Cell activity is altered by _________ specific enzymes. Inhibitors: Read this section and explain what “denatured” means: Denatured means: Factors that affect Enzyme Activity – hit reload to view the animations 1. Temperature. What happens if temperature is low? -What about if the temperature is high? 2. pH – What is the optimal or best pH for enzymes? - What happens if it is outside this range? Enzyme concentration- if it is increased what happens? And draw this graph-> Now contrast this with Substrate concentration; what happens? And draw graph belowà ~Congratulations you have completed your virtual online lab tour ©Harvey