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Plant Tissue Culture
What is
tissue
culture????
What is tissue culture????
1-Refers to technique of growing
plant cells, tissues, organs,
seeds
2-or other plant parts in a sterile
environment on a nutrient
medium
3-non-gene biotechnology to
regenerate complete plantlet from
isolated cells, or tissues or organ in
an artificially prepared nutrient
medium in the laboratory in aseptic
condition.
Basic need of tissue culture
1-nutrient medium
2-aseptic condition
3-aeration
nutrient medium
1-"culture medium" or "Basal medium" or
"growth medium"
2-depends on their nutrient medium
composition
3-supplies energy and essential metabolites to
growing tissues within it
4-state of culture medium solid = "Callus
culture" or liquid = "Suspension culture"
Ingredients of nutrient medium
1-carbon source (e.g. sucrose, glucose, starch
which supplies energy)
2-inorganic salts (major and minor elements),
3-vitamins,
4-hormones (auxin, cytokinin sometime GA).