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OIE Reference Laboratory Reports
Activities in 2011
Name of disease (or topic) for
which you are a designated OIE
Reference Laboratory:
Infectious bovine rhinotracheitis/
Infectious pustular vulvovagenitis
Address of laboratory:
Animal Health and Veterinary Laboratories Agency
(Formerly VLA),
New Haw, Addlestone,
Surrey KT15 3NB
UNITED KINGDOM
Tel.:
+44 (0)1932 341111
Fax:
+44 (0)1932 357239
e-mail address:
website:
[email protected]
www.defra.gov.uk/ahvla
Name (including Title and
Position) of Head of Laboratory
(Responsible Official):
Dr Catherine Brown
Chief Executive
Name(including Title and
Position) of OIE Reference
Expert:
Dr Malcolm Banks,
Consultant Virologist
Name (including Title and
Position) of writer of this report
(if different from above):
Annual reports of OIE Reference Centres, 2011
1
Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis
Part I: Summary of general activities related to the disease
1.
2.
Test(s) in use/or available for the specified disease/topic at your laboratory
Test
For
Specificity
Total
iELISA
Antibody
Whole virus
4,630
cELISA
Antibody
gB
16,036
cELISA
Antibody
gE
1,471
Bulk Milk iELISA
Antibody
Whole virus
1,246
Individual Milk iELISA
Antibody
Whole virus
27
VN (24H)
Antibody
Whole virus
629
FAT
Antigen
BoHV-1 antigen
3,099
BHK-21 cell culture
Virus isolation
Viable BoHV-1
1,322
Real time PCR
Viral DNA in semen
BoHV-1 DNA
105
Production and distribution of diagnostic reagents
Diagnostic reagents are prepared both for regional laboratories nationally, and for other countries. Materials
supplied include known positive and negative sera for use as test controls, FITC conjugated antiserum for viral
antigen detection, prepared positive and negative control slides for FAT, positive and negative semen samples for
the PCR and antigen for use in ELISA tests. Individual freeze dried BoHV-1 strains are also supplied on request.
Part II: Activities specifically related to the mandate
of OIE Reference Laboratories
3.
International harmonisation and standardisation of methods for diagnostic testing or the
production and testing of vaccines
a)
Establishment and maintenance of a network with other OIE Reference Laboratories
designated for the same pathogen or disease and organisation of regular inter-laboratory
proficiency testing to ensure comparability of results
None
b) Organisation of inter-laboratory proficiency testing with laboratories other than OIE
Reference Laboratories for the same pathogens and diseases to ensure equivalence of
results
The VLA conducts national and international proficiency testing for IBR assays. The following proficiency
tests are carried out for laboratories in the following countries:
PT0053 – IBR Serum ELISA Proficiency Test - 6 samples in each PT - is carried out 4 times annually for
laboratories in France, Austria, UK (6) Spain (2), S. Africa, Slovenia, New Zealand, Italy, Portugal (2),
Brazil, Denmark(3), Republic of Ireland (3), and Morocco(2), plus 2 AHVLA laboratories.
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Annual reports of OIE Reference Centres, 2011
Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis
PT0052– IBR Milk ELISA Proficiency Test - 5 samples in each PT - is carried out 4 times annually for
laboratories in Austria, UK (4) France, Norway, Republic of Ireland (3) plus 1 AHVLA laboratory.
PT0054 – Bovine respiratory virus package IBR/RSV/PI3 - Serum ELISA Proficiency Test - 4 samples in
each PT - is carried out 2 times annually for laboratories in Czech Republic, Republic of Ireland (2), UK (3)
Spain, Portugal and Morocco, plus 2 AHVLA laboratories.
PT0031 – Bovine respiratory virus package IBR/RSV/PI3 Antigen FAT slides Proficiency Test - 9 samples
in each PT - is carried out 3 times annually for laboratories in the UK (9) plus 13 AHVLA laboratories.
4.
Preparation and supply of international reference standards for diagnostic tests or vaccines
None
5.
Research and development of new procedures for diagnosis and control
A research project is underway to refine and improve the cost and throughput of the Real-time PCR for viral DNA
detection in bovine semen and tissue samples
6.
Collection, analysis and dissemination of epizootiological data relevant to international disease
control
A series of silent (subclinical) seroconversions to IBR virus in a UK bull stud are being investigated to determine
the true cause of the reactions. The seroconversions are atypical in that the transition from negative to positive is
extended to months rather than days, the conversion is shallow, reversible and in most cases confined to gB
blocking assays (ie SNT and indirect ELISA negative). The main suspicions are another, related herpesvirus or an
atypical BoHV-1 strain. The investigation is ongoing in collaboration with the OIE laboratory at the FLI, Reims,
Germany.
7.
Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the
pathogen and the disease concerned
Tests carried out within the AHVLA are assured by a comprehensive quality assurance system. The majority of
tests are subject to 3rd party accreditation by the United Kingdom Accreditation Service (UKAS) to the
internationally recognised ISO/IEC 17025 standard for laboratory competetence (AHVLA accreditation certificate
number 1769).
8.
Provision of consultant expertise to OIE or to OIE Member Countries
Consultant advice has been given, on request, to workers in Canada, The Republic of Ireland, India, Brazil, and
Egypt.
9.
Provision of scientific and technical training to personnel from other OIE Member Countries
None
10. Provision of diagnostic testing facilities to other OIE Member Countries
Primary diagnostic testing for serum antibody was provided on request to owners in Singapore, The Republic of
Ireland, Jersey and Guernsey.
11. Organisation of international scientific meetings on behalf of OIE or other international bodies
None
Annual reports of OIE Reference Centres, 2011
3
Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis
12. Participation in international scientific collaborative studies
Collaboration continued with the expert at the IBR reference laboratory at the FLI, Reims, Germany (Dr M Beer)
in relation to anomalous serum reactions with commercially available ELISA kits. These studies are ongoing.
13. Publication and dissemination of information relevant to the work of OIE (including list of
scientific publications, internet publishing activities, presentations at international conferences)

Presentations at international conferences and meetings
An invited seminar on IBR control in Europe was presented to vets, scientists, regulators and producers at a
meeting in Dublin.

Scientific publications in peer-reviewed journals
None

Other communications
A seminar focussed on IBR and Animal Breeding Controls was given to a cattle stakeholder group in the UK.
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Annual reports of OIE Reference Centres, 2011