Download Infectious hematopoietic necrosis

OIE Reference Laboratory Reports
Activities in 2010
Name of disease (or topic) for
which you are a designated OIE
Reference Laboratory:
Address of laboratory:
Infectious haematopoietic necrosis
Western Fisheries Research Center
6505 NE 65th Street, Seattle, WA 98115 USA
Tel.:
(+1-206) 526.65.87
Fax:
(+1-206) 526.66.54
e-mail address:
[email protected]
website:
Name of Head of Laboratory
(Responsible Official):
Name of OIE Reference Expert:
http://wfrc.usgs.gov/
Lyman Thorsteinson
James Winton
Name of writer of this report
(if different from above):
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
1
Infectious haematopoietic necrosis
Part I: Summary of general activities related to the disease
1.
Test(s) in use/or available for the specified disease at your laboratory
1. Virus isolation in cell culture (diagnosis/research 54 cases).
2. Virus identification using polyclonal and monoclonal antisera (diagnosis/research 0 cases).
3. Virus identification via hybridization with biotinylated probes (diagnosis/research 0 cases).
4. Virus identification via polymerase chain reaction (diagnosis/research 159 cases)
5. Virus identification and strain typing via sequencing G or N gene regions (diagnosis/research 133 cases).
6. Determination of viral load via quantitative PCR (diagnosis/research 157 cases).
2.
Production and distribution of diagnostic reagents
1. Testing and distribution of CHSE-214, EPC and other fish cell lines (5 cases).
2. Testing and distribution of polyclonal and monoclonal antisera to identify IHNV (2 cases).
3. Testing and distribution of biotinylated DNA probes to identify IHNV (0 cases).
4. Testing and distribution of reagents for polymerase chain reaction to identify IHNV (17 cases).
Part II: Activities specifically related to the mandate
of OIE Reference Laboratories
3.
4.
International harmonisation and standardisation of methods for diagnostic testing or the
production and testing of vaccines
1.
Prepared revised IHN chapter for OIE Diagnostic Manual for Aquatic Animal Diseases.
2.
Reviewed revised VHS chapter for OIE Diagnostic Manual for Aquatic Animal Diseases.
3.
Revised virology portion of new diagnostic manual for aquatic animal diseases for use by US entities.
4.
Invited member of task force for development of national aquatic animal health program for US.
Preparation and supply of international reference standards for diagnostic tests or vaccines
1. Testing and distribution of reference cell lines.
2. Testing and distribution of reference polyclonal and monoclonal antisera.
3. Testing and distribution of reference reagents for polymerase chain reaction.
5.
Research and development of new procedures for diagnosis and control
1. Development of monoclonal antibodies against IHNV.
2. Development of biotinylated DNA probes for IHNV.
2
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
Infectious haematopoietic necrosis
3. Development of polymerase chain reaction primer sets for IHNV.
4. Comparing genomic sequences of IHNV strains from throughout the world.
5. Identification of neutralizing epitopes on the IHNV glycoprotein.
6. Comparative challenge studies with various isolates of IHNV.
7. Killed vaccine studies in fish for control of IHN.
8. DNA vaccine studies in fish for control of IHN via genetic immunization.
9. Development of quantitative PCR assay for IHNV.
10. Development of quantitative PCR for trout cytokine genes involved in innate resistance to IHNV.
11. Development of strand-specific qPCR assay for messenger and genomic RNA species.
6.
Collection, analysis and dissemination of epizootiological data relevant to international disease
control
1. Provide data and professional advice to US Dept of Agriculture for OIE Annual Report.
7.
Provision of consultant expertise to OIE or to OIE Members
1. Provided advice to laboratory in Singapore
2. Provided advice to laboratory in China
3. Provided advice to laboratory in India
4. Provided advice to laboratory in Taiwan
5. Provided advice to laboratory in Korea
8.
Provision of scientific and technical training to personnel from other OIE Members
1. Hosted personnel from laboratories in Canada, Norway, Philippines, Korea.
9.
Provision of diagnostic testing facilities to other OIE Members
–
10. Organisation of international scientific meetings on behalf of OIE or other international bodies
–
11. Participation in international scientific collaborative studies
1. Participated in collaborative study with personnel from Department of Fisheries and Oceans, Canada.
2. Participated in collaborative study with personnel from National Veterinary Laboratory, Denmark.
3. Participated in collaborative study with personnel from National Veterinary Laboratory, Norway.
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
3
Infectious haematopoietic necrosis
12. Publication and dissemination of information relevant to the work of OIE (including list of
scientific publications, internet publishing activities, presentations at international conferences)

Scientific publications in peer-reviewed journals
Jonstrup, S.P., H. Schuetze, G. Kurath, T. Gray, B.B. Jensen and N.J. Olesen (2010) An isolate and sequence
database of infectious hematopoietic necrosis virus (IHNV). J. Fish Dis. 33:469-471.
Kurath, G., K.A. Garver, S.E. LaPatra and M.K. Purcell (2010) Resistance and protective immunity in Redfish
Lake sockeye salmon exposed to M type infectious hematopoietic necrosis virus (IHNV). J. Aquat. Anim. Health.
22:129-139.
Kurath, G., K.A. Garver, M.K. Purcell, M.M. Peñaranda, R. Life, and S.Rudakova. (in review) Host specificity
and ecology of IHN virus in Pacific salmonids.
Palti, Y., S. Gahr, M. Purcell, S. Hadidi, C. Rexroad III and G. Wiens (2010) Identification, characterization and
genetic mapping of TLR7, TLR8a and TLR8b genes in rainbow trout (Oncorhynchus mykiss). Dev. Comp.
Immunol. 34:219-233.
Palti, Y., M.F. Rodriguez, S.A. Gahr, M.K. Purcell, C.E. Rexroad III, G.D. Wiens (2010) Identification,
characterization and genetic mapping of TLR1 loci in rainbow trout (Oncorhynchus mykiss). Fish Shellfish
Immunol. 28:918-926.
Park, J.W., C.H. Moon, A.R. Wargo, M.K. Purcell and G. Kurath (2010) Differential growth of U and M type
infectious hematopoietic necrosis virus in a rainbow trout derived cell line, RTG-2. J. Fish Dis. 33:583-591.
Polinski, M.P., J.D. Drennan, W.N. Batts, S.C. Ireland and K.D. Cain (2010) Establishment and partial
characterization of a cell line from burbot Lota lota maculosa: susceptibility to IHNV, IPNV and VHSV. Dis.
Aquat. Org. 90:15-23.
Purcell, M.K., S.E. LaPatra, J.C. Woodson, G. Kurath, and J.R. Winton (2010) Early viral replication and induced
or constitutive immunity in rainbow trout families with differential resistance to Infectious hematopoietic necrosis
virus (IHNV). Fish Shellfish Immunol. 28:98-105.
Walker, P.J. and J.R. Winton (2010) Emerging virus diseases of fish and shrimp. Vet. Res. 41:51,
doi:10.1051/vetres/2010022.
Wargo, A.R., K.A. Garver and G. Kurath (2010) Virulence correlates with fitness in vivo for two M group
genotypes of Infectious hematopoietic necrosis virus (IHNV). Virology 404:51-58.
Winton, J., W. Batts, P. deKinkelin, M. LeBerre, M. Bremont and N. Fijan (2010) Current lineages of the
Epithelioma papulosum cyprini (EPC) cell line are contaminated with fathead minnow (Pimephales promelas) cells.
J. Fish Dis. 33:701-704.
13. Inscription of diagnostic kits on the OIE Register
i)
Did you participate in expert panels for the validation of candidate kits for inscription on the
OIE Register?
No.
ii)
Did you submit to the OIE candidate kits for inscription on the OIE Register?
No.
_______________
4
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010