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Consulta: creatorFacets:"Pereira Jr,Nei" Registros recuperados: 6 Data/hora: 09/06/2017 03:35:02 Effects of agitation and exogenous H2 on bioconversion of sugarcane bagasse into ethanol by Clostridium thermocellum ATCC 27405 Provedor de dados: 69 Autores: Groposo,Claudia; Castro,Aline Machado de; Pereira Jr,Nei. Background: The production of ethanol by a Consolidated Bioprocessing (CBP) strategy, which simultaneously combines cellulase production, lignocellulosic biomass hydrolysis and fermentation of released sugars to ethanol in one bioreactor, is a promising technology for cost reduction in the biological processing of biomass, specially using agroindustrial residues. Clostridium thermocellum is an anaerobic, thermophilic, strictly fermentative gram positive bacterium that meets all the requirements for CBP. Results: Ethanol concentration obtained in the non-stirred fermentation process in flasks with raw bagasse was two times greater than that in the stirred system. The results observed using a pretreated sugarcane bagasse in non-stirred flasks regarding... Tipo: Journal article Palavras-chave: Consolidated bioprocessing; Pretreated sugarcane bagasse; Raw sugarcane bagasse; Thermophilic. Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600009 Investigation of cultivation conditions for capsular polysaccharide production by Streptococcus pneumoniae serotype 14 Provedor de dados: 69 Autores: Moura Leal,Maria de Lourdes; da Silva Gomes Pereira,Denise; Jessouroun,Ellen; Peixoto Gimenes Couto,Maria Antonieta; Pereira Jr,Nei. Streptococcus pneumoniae (pneumococcus) is among the most significant causes of bacterial disease in humans. Capsular polysaccharide (CPS) production is essential for pneumococcal virulence. Pneumococcal CPS has been widely used as vaccine antigen. This study is focused on the influence of culture conditions of Streptococcus pneumoniae serotype 14 as for developing an industrial method for polysaccharide production. The pH proved to be a highly important variable in batchwise culture. Using the pH control all glucose added was consumed resulting in a four-fold increase in polysaccharide productivity relative to cultivation without pH control. S. pneumoniae is a lactic acid bacterium, so named for its primary metabolic byproduct (lactate), which has an... Tipo: Journal article Palavras-chave: Polysaccharide capsular; Vaccine; Streptococcus pneumoniae. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500006 Production and properties of the cellulase-free xylanase from Thermomyces lanuginosus IOC-4145 Provedor de dados: 58 Autores: Damaso,Mônica Caramez Triches; Andrade,Carolina M.M. Carvalho; Pereira Jr,Nei. In recent years, xylanases have expanded their use in many processing industries, such as pulp and paper, food and textile. Thermomyces lanuginosus IOC-4145 was able to produce a very high level of cellulase-free xylanase in shaken cultures using corncob as substrate (500 U/mL). An optimization of the medium composition in submerged fermentation was carried out aiming at a low cost medium composition for enzyme production. Statistical experiment design was employed for this purpose, pointing out corncob as the most important parameter, which affects enzyme production. Additionally, the influence of several chemicals on xylanase activity was investigated in the crude extract. A slight stimulation of the enzyme (5-15%) was achieved with NaCl and urea, both... Tipo: Journal article Palavras-chave: Xylanases; Thermomyces lanuginosus; Corncob; Submerged fermentation; Medium optimization. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400011 Simultaneous saccharification and fermentation process of different cellulosic substrates using a recombinant Saccharomyces cerevisiae harbouring the β-glucosidase gene Provedor de dados: 69 Autores: Ferreira,Verônica; Faber,Mariana de Oliveira; Mesquita,Sabrina da Silva; Pereira Jr,Nei. In Brazil, the production of ethanol from sugarcane produces large amounts of lignocellulosic residues (bagasse and straw), which have been driving research and development for the production of second generation ethanol. In the present work, a recombinant Saccharomyces cerevisiae strain expressing the β-glucosidase gene from Humicola grisea was used for ethanol production from three different cellulosic sources by simultaneous saccharification and fermentation. Initially, a enzymatic pre-hydrolysis step was done with a solid:liquid ratio of 1:4, and an enzymatic load of 25 filter paper activity (FPU).g-1 of cellulosic substrate. Using sugarcane bagasse pretreated cellulignin, crystalline cellulose and carboxymethyl cellulose, 51.7 g L-1, 41.7 g... Tipo: Journal article Palavras-chave: Bioethanol; Lignocellulosic biomass; Sugarcane bagasse derived cellulignin. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200005 Sugar cane bagasse as feedstock for second generation ethanol production: Part I: Diluted acid pretreatment optimization Provedor de dados: 69 Autores: Vargas Betancur,Gabriel J; Pereira Jr,Nei. Tons of sugar cane bagasse are produced in Brazil as waste of the sugar and ethanol industries. This lignocellulosic material is a potential source for second-generation ethanol production. Diluted acid hydrolysis is one of the most efficient pretreatments for hemicellulosic solubilization. The hydrolysate obtained is rich in xylose, which can be converted to ethanol by Pichia stipitis. This work used a statistical approach and the severity factor to investigate the effects of factors associated with the diluted acid hydrolysis process (acid concentration, solid:liquid ratio and time of exposure) on various response variables (xylose concentration, hydrolysis yield, inhibitor concentration and hydrolysate fermentability). The severity factor had a strong... Tipo: Journal article Palavras-chave: Hemicellulose hydrolysis; Severity factor; Xylose. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300010 Sugar cane bagasse as feedstock for second generation ethanol production: Part II: Hemicellulose hydrolysate fermentability Provedor de dados: 69 Autores: Betancur,Gabriel J. Vargas; Pereira Jr,Nei. Sugar cane bagasse is produced in Brazil as waste of the sugar and ethanol industries. This lignocellulosic material is a potential source for second-generation ethanol production; however a pretreatment stage is essential, which aims at removing the hemicellulose component by disorganizing the lignocellulosic complex. In this work sugar cane bagasse was pretreated by diluted acid hydrolysis resulting in xylose-rich hydrolysates, which could be fermented to ethanol by a strain of the yeast Pichia stipitis. Statistical approach was used to investigate the effects of factors associated with the diluted acid hydrolysis process (acid concentration, solid:liquid ratio and time of exposure) on the fermentability of different hydrolysates. The statistical... Tipo: Journal article Palavras-chave: Bioethanol; Pichia stipitis; Xylose fermentation. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500014