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PC2 LABORATORY INSPECTION CHECKLIST Inspection Team: Laboratory Room No.: Laboratory Supervisor: Inspection Date: NB: Boxes to be ticked as items are sighted () or not present (), or otherwise NA indicates the item is not applicable to this laboratory. No. 1 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 1.10 1.11 1.12 1.13 1.14 2 2.1 2.2 2.3 2.4 Question / / NA Corrective Actions Responsible Person Target Completion Date Facilities Is there appropriate signage on entrances to the laboratory? (a) Biohazard symbol (b) Level of containment (c) Access restrictions Are there stations for emergency: (a) eyewashing (b) safety showers Are ceilings, walls and floors smooth, easy to clean, impermeable to liquids, and resistant to commonly used reagents and disinfectants? Do floors have a non-slip finish? Have internal fittings and fixtures been fitted to minimize the collection of dust? Are there hands free basin mixers for handwashing near to laboratory exits? Is the water supplied to the laboratory fitted with backflow prevention? Is there a directional air flow maintained by extracting room air? Is there a pressure steam sterilizer for sterilizing infectious laboratory waste? Are there biohazard stickers posted on storage units which are kept outside the designated laboratory for microorganisms or recombinant or manipulated DNA ? Are there suitable coat hooks available for every individual’s laboratory gown? Are the hooks adjacent to the laboratory access door? Are protective covers kept on keyboards which are housed on work benches? Is there a supply of clearly labelled disinfectants for decontamination purposes? Are containers for infectious materials available? PPE Does protective clothing afford the protection to the front part of the body? Is footwear completely enclosed? Are gloves worn: (a) when working in a biological safety cabinet? (b) when handling human blood and bodily fluids? Are goggles or visors worn: (a) to protect eyes from contaminated or The University of Western Australia PC2 Laboratory Inspection Checklist Page 1 of 2 Created: January 2009 Last Review: Next Review: Hardcopies of this document are considered uncontrolled please refer to UWA Safety and Health website for latest version No. 3 3.1 3.2 3.3 4 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 4.10 Question / / NA Corrective Actions Responsible Person Target Completion Date dangerous materials, or from UV light? (b) when subculturing organisms in a biological safety cabinet? Containment Equipment Is a biological safety cabinet used for manipulations where aerosols may be produced? Are biosafety cabinets serviced and certified annually? Are centrifuges fitted with either sealed rotors or safety cups where large volumes or high concentrations of infectious material is being used? Work Practices Is access to the laboratory limited to laboratory personnel and persons specified by laboratory management? Are doors kept closed while laboratory work is in progress? Have laboratory personnel received instruction and training, with regular updates, in handling pathogens? Are sharps being disposed of in an approved sharps containers? Are maintenance and service personnel advised of the special microbiological hazards in the laboratory? Are potentially contaminated surfaces disinfected before maintenance of equipment is conducted? Are containers of viable microorganisms transported between laboratories within a second unbreakable closed container which can be readily decontaminated? Is waste disposed of appropriately? Is special care taken when handling human blood, serum, other bodily fluids and substances? Are separate work areas within the laboratory provided for reference documents, papers and writing space? Has a pest control program against insects, birds and animals been instituted? Other Comments : Definitions PC1/PC2: Physical Containment Level 1 or 2 PPE: Personal Protective Equipment The University of Western Australia PC2 Laboratory Inspection Checklist Page 2 of 2 Created: January 2009 Last Review: Next Review: Hardcopies of this document are considered uncontrolled please refer to UWA Safety and Health website for latest version