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CURRICULUM VITAE
OF
A.H.M. KHURSHID ALAM
CAREER OBJECTIVE
To build up career in Teaching and Research sector in any reputed University of the World.
PRESENT STATUS
Lecturer, Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh.
ACADEMIC QUALIFICATIONS
M. Pharm. 1997
Subject studied
Thesis Title
Thesis Supervisor
Medium of Instruction
B. Pharm. 1996
Subject studied
Medium of Instruction
H.S.C. 1992
Subject studied
S.S.C. 1990
Subject studied
Completed one year M. Pharm. course from the Department of
Pharmacy, Faculty of Science, University of Rajshahi, Rajshahi,
Bangladesh in 1997 (examination held in 2000, due to session jam).
Secured first class (Second position) with 68.33% marks.
Advanced Pharmaceutical Analysis, Medicinal Chemistry,
Pharmacology and Toxicology, Advanced Pharmaceutical Technology
and Cosmetology and Pharmaceutics.
Antishigella activity of Hemigraphis hirta T. Anders and Achyranthes
ferruginea Roxb.
Dr. Md. Golam Sadik, Associate Professor, Department of Pharmacy,
University of Rajshahi, Bangladesh.
E-mail: [email protected].
English
Completed three years B. Pharm. (Hons.) course from the Department of
Pharmacy, Faculty of Science, University of Rajshahi, Rajshahi,
Bangladesh in 1996 (examination held in 1998, due to session jam).
Secured first class (fourth position) with 60.93% marks.
Inorganic, Organic Physical Pharmaceutical Chemistry, Pharmacognosy,
Physiology and Biochemistry, Pharmaceutical Microbiology, Statistics,
Pharmacology, Pharmaceutics, Pharmaceutical Analysis and Quality
Control, Medicinal Chemistry, Industrial and Hospital Pharmacy,
Pharmacokinetics and Biopharmaceutics.
English
Passed two years Higher Secondary Certificate (H.S.C.) examination
from Bagher Para Degree College, Jessore, Bangladesh in 1992.
Obtained 1st division with 68.3% marks.
Bengali (Language and Literature), English (Language and Literature),
Physics, Chemistry, Mathematics and Biology (Botany and Zoology).
Passed ten years Secondary School Certificate (S.S.C.) examination
from Bagher Para Pilot High School, Jessore, Bangladesh in 1990.
Obtained 1st division with 68.5% marks.
Bengali (Language and Literature), English (Language and Literature),
General Science, Mathematics (General and Higher), Geography and
Book keeping.
RESEARCH EXPERIENCE
Completed two years research work on Phytochemistry entitle Antishigella activity of Hemigraphis
hirta T. Anders and Achyranthes ferruginea Roxb. and submitted a dissertation in partial fulfillment of
the requirements for the degree of M. Pharm. to the department of Pharmacy, University of Rajshahi.
Now I am doing my research on experimental animal models of human autoimmune disease,
Rheumatoid Arthritis (RA).
WORK DONE ON THE RESEARCH PERIOD
1.
Extraction of whole plants of Hemigraphis hirta T. Anders and Achyranthes ferruginea Roxb. by
rectified spirit with the help of hot and cold extraction methods respectively.
2.
Fractionation of the crude extract using petroleum ether, chloroform, ethyl acetate and methanol.
3.
Isolation and purification of compounds using column chromatography, TLC, PTLC
4.
Structure elucidation of the isolated compounds with the help of UV, IR, NMR and Mass
Spectroscopy.
5.
Observation of in vitro antishigella and other antimicrobial activity of crude extracts, fractions and
pure compounds with standard one using disc diffusion assay method.
6.
Determination of Minimum Inhibitory Concentration (MIC) of pure compounds and fractions.
7.
Brine shrimp lethality bioassay and determination of LC50 value of various fractions and pure
compounds.
8.
Observation of subacute toxicity of pure compounds and crude extract in rat.
ACHIEVEMENT OF RESEARCH



Four compounds were isolated, of which one is N-trans-feruloyl-4-methyl dopamine isolated from
Achyranthes ferruginea. Although the compound N-trans-feruloyl-4-methyl dopamine is a known
natural product but this is the first report of its occurrence from the Amaranthaceae family and
second report from the plant source. The remaining three compounds were known, designated as
squalene, lupeol and -sitosterol- -D-glucoside.
The compound, N-trans-feruloyl-4-methyldopamine showed good antibacterial activity.
Biochemical parameters showed normal on subacute studies on rats with the compounds and
fractions.
TRAINING OBTAINED
Two weeks in-plant training in “Square Pharmaceutical Limited” a leading chemical and pharmaceutical
industry in Bangladesh. During this period I observed manufacturing as well as quality control operation of
tablets, capsules, liquid and sterile preparations.
LIST OF PUBLICATIONS
INTERNATIONAL PUBLICATIONS
1. Yamaki, K, Alam, AHMK, Hossain, MA, Taneda, S, Yanagisawa, R, Takano, H and Yoshino, S. Effect
of Rolipram, a Phosphodiesterase IV Inhibitor, on Allergic Footpad Swelling using Various Adjuvants in
Mice. Scand. J. Immunol. 2005, 62(4): 378-84.
Abstract: We studied the effect of rolipram, a phosphodiesterase (PDE) IV inhibitor, on allergic footpad
swelling in mice. For this study, varying adjuvants including complete Freund's adjuvant (CFA),
incomplete Freund's adjuvant (IFA) and Imject Alum (Alum) were used because the extent of antigenspecifically induced T helper type 1 (Th1) and Th2 responses had been shown to depend on adjuvants used.
To induce allergic footpad swelling, we immunized mice with ovalbumin (OVA) emulsified in either CFA
or IFA, dissolved in Alum or in phosphate-buffered saline (PBS) as a control (day 0), followed by
subcutaneous injection of the antigen into footpads on day 21. Rolipram was given orally to the animals
daily from days 0-20. Results showed that treatment with rolipram was followed by an increase in early
swelling at 0.5 h and a decrease in late swelling at 6 and 24 h in the CFA group. In the IFA group, rolipram
significantly enhanced swelling at, but not after, 30 min. In the Alum and the PBS groups, the PDE
inhibitor failed to affect the OVA-specific footpad reaction at all times examined. Treatment of the CFA
and IFA groups with rolipram significantly inhibited the production of the Th1 antibody anti-OVA
immunoglobulin G2a (IgG2a), and the drug enhanced Th2 cell-dependent anti-OVA IgE production. In
both groups, rolipram also enhanced the secretion of Th2 cytokines including interleukin-4 (IL-4) and IL10. These findings suggest that rolipram may facilitate early allergic footpad swelling mediated by Th2
immune responses, while the late phase of swelling associated with Th1 responses may be attenuated by the
PDEIVinhibitor.
2. Rahman, M.M., Lopa, S.S., Sadik,G., , Rashid, M.H., Islam, R., Khondkar, P., Alam, A.H.M.K and
Rashid, M. A. Antimicrobial and cytotoxic compounds from the bark of Cananga odorata. Fitoterapia;
2005, 76:758-761.
Abstract: O-methylmoschatoline, liriodenine and 3,4-dihydroxybenzoic acid isolated from the barks of
Cananga odorata showed significant antibacterial activities against a number of Gram (+) and Gram (-)
bacteria.
3. Salam, K.A., Hossain, A.K.M.M., Alam, A.H.M.K., Pervin, F. and Absar, N.A. Comparative Analysis
on Physico-chemical Characteristics of Oil Extracted from Six Different Parts of Hilsa fish (Hilsa ilisha).
Pakistan Journal of Biological Sciences, 2005, 8(6): 810-815.
Abstract: The physico-chemical characteristics of oils extracted from six different parts of hilsa fish (Hilsa
ilisha) like dorsal, ventral, tail, egg, liver and brain were analyzed. The physical characteristics such as the
specific gravity, refractive index, smoke point, flash point, fire point, cloud point, solidification point and
pour point of the hilsa fish oils from the different parts presently examined were ranged from 0.920 to
0.932, 1.4700 to 1.4722 at 25, 220 to 228 0C, 322 to 3300C, 350 to 3640C, 2 to 2.40C, -10 to -50C and -7 to
60C, respectively. The chemical properties such as saponification value, iodine value, peroxide value, acid
value, % FFA and unsaponifiable matters of the hilsa fish oils from different parts were found to be varied
from 180.28 to 194.00, 80.70 to 126.40, 7 to 10, 4.16 to 12.00, 2.08 to 6.00 and 1.58% to 7.00%,
respectively. The saturated and unsaturated fatty acids present in the oil samples were mainly myristic acid
(5.44 to 7.24%), palmitic acid (22.00 to 27.08%), stearic acid (4.00 to 6.32%), palmitolenic acid (12 to
14%), oleic acid (26.08 to 29.78%), linoleic acid (0.92 to 2.20%) and linolenic acid (0.82 to 1.08%). The
storage effect on the hilsa fish oils, which were obtained from different parts for the production of fatty
acids by the action of lipase have been studied after storing the samples at low temperature (-100C- 00C)
and at room temperature (25-280C). The contents of % FFA were initially low but increased rapidly on
storage. It has been shown that lipase enzyme in hilsa fish oil is active even at temperature -100C. The
hydrolytic deterioration of hilsa fish oils were found to be more effective at 00C than that from -100C.
Further, the qualities of hilsa fish oils were deteriorated slightly further when stored at 25 0C.
4. Baki, M.A., Akhtar, N., Rahman, M.M., Islam, M.N., Hossain, M., Islam, N., Alam, A.H.M.K., Islam,
R., Khatun, N.A. and Mondal, K.A.M.S.H. Synergistic action of Wedelia calendulaceae Less. plant
extracts with lamda cyhalothrin on adult red flour bettle Tribolium castaneum herbst. Journal of Agronomy;
2005, 4 (1): 18-25.
Abstract: Synergistic action of Wedelia calendulaceae Less. plant extracts combined with Lamda
cyhalothrin were demonstrated against red flour bettle T castaneu in methanol extract W calendulaceae
plant extracts offered Synergistic action when used Lamda cyhalothrin. It was noted that plant extract
indicates
synergistic
action
from
1:1
to
1:5
ratio
and
above.
5. Yamaki, K., Li, X., Uchida, H., Alam, A.H.M.K., Hossain, M.A., Yanagisawa, R., Takano, H., Hayashi,
H., Mori, Y. and Yoshino, S. Effects of the phosphodiesterase IV inhibitor rolipram on Th1 and Th2
immune responses in mice. Journal of Pharmacy and Pharmacology; 2004, 56: 877-882.
Abstract: The present study was designed to investigate the effect of the phosphodiesterase (PDE) IV
inhibitor rolipram on Th1 and Th2 immune responses in mice. Mice were immunized subcutaneously at the
base of the tail with ovalbumin (OVA) emulsified with complete Freund's adjuvant (day 0) and were
treated daily with oral administration of various doses of rolipram from days 0 to 20. On day 21, production
of anti-OVA IgG and proliferative responses to the antigen were determined. Anti-OVA IgG2a and
interferon- (IFN-) as indicators of Th1 responses and anti-OVA IgG1 and interleukin-10 (IL-10), as
indicators of Th2 responses, were also measured. The results showed that treatment with rolipram failed to
affect the production of OVA-specific IgG but decreased the proliferation of spleen cells to the antigen. Its
inhibitory effect on these immune responses was correlated with a marked decrease in IFN- and but not
IL-10 production, although neither anti-OVA IgG2a nor IgG1 production was affected by rolipram. These
results suggest that rolipram may preferentially inhibit Th1 responses more effectively than Th2 responses.
Administration of rolipram resulted in suppression of antigen (OVA)-induced arthritis in mice. The
suppression of joint inflammation by rolipram was associated with the inhibition of OVA-specific
proliferative responses of spleen cells and IFN- secretion. These results indicate that rolipram may be
effective in regulating Th1 mediated diseases such as rheumatoid arthritis.
6. Islam, R., Alam, A.H.M.K., Hossain, M.A., Mosaddik, M.A. and Sadik, G. Biological screening of
Bangladeshi mango mistletoe (Loranthus globosus Roxb.) bark extracts. Fitoterapia 2004, 75:
405-408.
Abstract: The ethyl acetate extract of the Bangladeshi mango mistletoe (Loranthus globosus Roxb.)
bark was found to be most effective against both gram positive and gram-negative bacteria and it also
showed good cytotoxicity with a LC50 10.83 g/ml.
7. Hossain, M.S., Hossain, A., Islam, R., Alam, A.H.M.K., Zahan, K., Sarkar, S. and Farooque, M.A.
Antimicrobial and cytotoxic activities of 2- aminobenzoic acid and 2-aminophenol and their coordination
complexes with Magnesium (Mg-II). Pakistan Journal of Biological Sciences; 2004, 7 (1): 25-27.
Abstract: Owing to the biological importance of metalloelements in many biological processes, especially
metabolic processes, magnesium (Mg-II) complexes were synthesized and examined for their antimicrobial
and cytotoxic activities. Among the two synthesized organometalic complexes [Mg (2-ap)2, A] and [Mg (2ab)2, C], the maximum antibacterial and antifungal activities were shown by the compound A. Among the
ligands, 2-aminophenol (B) showed more antibacterial activity than 2- aminobenzoic acid (D). The
minimum inhibitory concentration for the complex A against five pathogenic bacteria Streptococcus-haemolyticus, Bacillus subtilis, Staphylococcus aureus, Salmonella typhi and Escherichia coli were found
in the range of 32-64 g ml-1. The complexes were also tested for their cytotoxicity using brine shrimp
lethality bioassay method and the LC50 values of the complexes A and C were found to be 5.7 and 24.3 g
ml-1, respectively. Antibacterial, antifungal and cytotoxic studies undertaken for the above compounds
indicated structure-activity relationships. These metalloderivatives were more active than the parent
compounds (ligands).
8. Alam, A.H.M.K., Sadik, G., Rashid, M.H., Hasan, C.M. and Rashid, M.A. N-trans-feruloyl-4methyldopamine from Achyranthes ferruginea. Biochemical Systematics and Ecology; 2003, 31(11): 134546.
Abstract: N-trans-feruloyl-4-methyldopamine was isolated from Achyranthes ferruginea as a new source. This
compound has got chemotaxonomic significance in the family Amaranthaceae.
9. Alam, A.H.M.K., Rahman, M.A.A., Baki, M.A., Rashid, M.H., and Sadik, G. Chemical constituents of
Hemigraphis hirta T. Anders (Acanthaceae). Pakistan Journal of Biological Sciences; 2002, 5(11): 12641266.
Abstract: Phytochemical examination of the petroleum ether extract of Hemigraphis hirta has led to the
isolation of squalene (1), lupeol (2) and -sitosterol (3) by a combination of column and preparative thin
layer chromatography. The structures of these compounds were determined by spectroscopic analysis (UV,
IR, 1H-NMR, 13C-NMR) as well as by comparison of their spectral data with previously reported values.
10. Sadik, G., Gafur, M.A., Bhuiyan, M.S.A., Alam, A.H.M.K., Biswas, M.H.U., Hasan, P., Mannan,
A.M, Khan, M.O.F. and Chowdhury, A.K.A. Antifertility Activity of Pergularia daemia. The sciencess;
2001, 1 (1): 22-24.
Abstract: The ethanolic extract of Pergularia daemia and its steroidal fraction were studied for antifertility
activity. Both the ethanol extract and the steroidal fraction showed significant antifertility activity in the
pre-implantation stage in female mice. The ethanol extract also showed late abortifacient activity.
11. Alam, A.H.M.K., Islam, R, Salam, K.A., Baki, M.A., Manir, M., Hossain A, and Sadik, G.
Toxicological studies of N-trans-feruloyl-4-methyldopamine isolated from Achyranthes ferruginea.
Pakistan Journal of Biological Sciences (In-press).
Abstract: The subacute toxicity of a metabolite N-trans-feruloyl-4-methyldopamine isolated from
Achyranthes ferruginea (Amaranthaceae) was studied on long Evan’s rats. The studies include the gross
observation such as changes in body weight, haematological profiles [total count of red blood cells (RBC)
and white blood cell (WBC), differential count of WBC, platelet count and haemoglobin (Hb) %],
biochemical parameters of blood [serum glutamate oxaloacetate transaminase (SGOT), serum glutamate
pyruvate transaminase (SGPT), serum alkaline phosphatase, urea and creatinine] and histopathology of the
liver, kidney, heart and lung of both control and experimental groups of rats. The changes of
haematological and biochemical parameters were statistically not significant after the administration of
metabolite in a dose of 300 g/rat/day for consecutive 14 days. No abnormalities were also found in the
histopathology of the liver, kidney, heart and lung in the experimental group of rats following same dose
when compared with the control group. This preliminary study suggests that the plant metabolite may be
used safely for clinical trial.
NATIONAL PUBLICATIONS
1. Lopa, S.S., Sadik, G., Rahman, M.M., Rashid, M.H., Islam, R., Khondkar, P., Alam, A.H.M.K and
Rashid, M.A. Oxoaporphinoids and a benzoic acid derivative from Cananga odorata. Dhaka Univ. J.
Pharm. Sci., 2004, 3 (1&2): 1-4.
Abstract: O-methylmoschatoline (1), liriodenine (2) and 3,4-dihydroxybenzoic acid (3) were isolated from
the stem bark of Cananga odorata. The structures of compounds were confirmed by a series of ID and 2D
NMR and MS analyses. The chemotaxonomic significance of these compounds has also been discussed.
2. Rashid, M.H., Rahman, M.A.A., Gafur M.A., Sadik, G., Alam, A.H.M.K., Sugimoto, M., Chowdhury
R. and Rashid, M.A. Chemical Constituents of Ipomoea turpethum. Dhaka Univ. J. Pharm. Sci. 2003, 2(2):
73-76.
Abstract: Chromatographic separation and purification of the organic solvent extracts of Ipomoea
turpethum stem bark afforded a total of three compounds, daucosterol (1), salicylic acid (2) and N-p-transcoumaroyltyramine (3). The structures of these compounds were determined by spectroscopic analyses,
including 1H and 13C NMR, 1H-1H COSY, HMQC, HMBC and mass spectroscopy. These compounds are
hitherto unknown from I. Turpethum.
3. Alam, A.H.M.K., Rahman, M.A.A., Bhuiyan, M.S.A., Gafur, M.A. and Sadik, G. Antimicrobial and
cytotoxic activities of the extracts of Hemigraphis hirta T. Anders. Bangladesh Pharmaceutical Journal;
2002,12(4): 11-14.
Abstract: The in vivo antimicrobial activity of crude petroleum ether, chloroform, ethyl acetate and
methanol extracts of a local antidiarrhoeal plant Hemigraphis hirta (family Acanthaceae), was screened
against fourteen (five gram-positive and nine gram-negative) pathogenic bacteria and three pathogenic
fungi using the disk diffusion method. The crude petroleum ether and methanol extracts showed strong
activity against all Shigella species and mild to moderate activity against other pathogenic bacteria and
fungi, whereas the chloroform and ethyl acetate extracts showed mild to moderate activity against bacteria
and fungi. All the extracts were found to be inactive against Candida species. The cytotoxic activity was
measured by brine shrimp lethality bioassay. The LC50 values of the petroleum ether, chloroform, ethyl
acetate and methanol extracts were found to be 9.77, 9.77, 9.74 and 10 g/ml respectively.
4. Alam, A.H.M.K., Rahman, M.A.A., Baki, M.A., Rashid, M.H., Bhuiyan, M.S.A., and Sadik, G.
Antidiarrhoeal principles of Achyranthes ferruginea Roxb. and their cytotoxicity. Bangladesh
Pharmaceutical Journal; 2002,12(4): 1-4.
Abstract: The plant Achyranthes ferruginea (family Amaranthaceae) has folkloric reputation in the
treatment of diarrhoeal diseases. The present investigation was done to observe the effects of different
extracts of the plant against six diarrhoea-producing bacteria as well as some other pathogenic bacteria and
fungi. The crude chloroform extract showed satisfactory results against all Shigella species and other
pathogenic bacteria whereas methanol and ethyl acetate extracts showed mild to moderate activity. The
chloroform extract also exhibited potent antifungal activities. The LC 50 values of the petroleum ether,
chloroform, ethyl acetate and methanol extracts were found to be 12.58, 16.21, 7.07 and 11.74 g/ml,
respectively by brine shrimp lethality bioassay.
SUBMITTED PAPERS
1. Alam, A.H.M.K., Sadik, G., Islam, R., Khondhker, P., Hossain, M.A., Rashid, M.A. and Rahman, M.M.
Antimicrobial and Cytotoxic Activities of Achyranthes ferruginea. Fitoterapia
2. Yamaki, K., Li, X., Uchida, H., Alam, A.H.M.K., Hossain, M.A., Yanagisawa, R., Takano, H., Hayashi,
H., Mori, Y. and Yoshino, S. Differential effect of the phosphodiesterase IV inhibitor rolipram and varying
types of anti-arthritic drugs on collagen-induced arthritis in mice. Journal of Pharmacy and Pharmacology
3. Yamaki, K., Li, X., Uchida, H., Alam, A.H.M.K., Hossain, M.A., Yanagisawa, R., Takano, H., Hayashi,
H., Mori, Y. and Yoshino, S. Differential effect of the phosphodiesterase IV inhibitor rolipram and varying
types of anti-arthritic drugs on antigen-induced arthritis. Journal of Pharmacy and Pharmacology
JOB EXPERIENCE
From February 31,2001 to November 03,2002
From November 05, 2002 till to date
OTHER EXPERIENCES
Computer Skill
As product Executive in Nipa pharmaceutical Ltd.,
Mohammadpur, Dhaka, Bangladesh. I was responsible
for medical representative training, product wise sale
planning and design, new product entry, insert, cartoon
and label preparation.
As Lecturer, Department of Pharmacy, University of
Rajshahi, Bangladesh.
Well conversant with popular package like MS Word, MS Excel.
LANGUAGES PROFICIENCY
English (speaking, writing, reading), Bengali (speaking, writing, reading)
MEMBERSHIP OF PROFESSIONAL BODIES
Registered Pharmacist of Pharmacy Council of Bangladesh (BPC)
Active member of Pharmacy Graduates Association (PGA)
Bangladesh Pharmaceutical Society (BPS)
Rajshahi University Teachers Association (RUTA)
Ex. active member, Assistant General Secretary (AGS) and General Secretary (GS) of Rajshahi University
Pharmacy Association (RUPA)
REFEREES
1.Dr.M.Golam Sadik
Associate Professor
Department of Pharmacy
University of Rajshahi
Rajshahi-6205.Bangladesh.
E-mail: [email protected]
2.Dr.M.Aslam Hossain
Chairman and Associate Professor Department of
Pharmacy
University ofA Rajshahi
Rajshahi-6205.Bangladesh.
E-mail: [email protected]
PERSONAL DATA
Father’s Name
Date of birth
Nationality
Place of birth
Late Abul Hossain
September 14, 1975
Bangladeshi (by birth)
Jessore, Bangladesh
Religion
Marital Status
Sex
Islam
Married
Male
MAILING ADDRESS
Lecturer
Department of Pharmacy
University of Rajshahi, Rajshahi-6205.Bangladesh
Tel (Office): 88-0721-750041-4110
Tel (Residence): 88-0721-750456
Telefax: 88-0721-750064
E-mail: [email protected]
HOME ADDRESS
C/O
: Late Abul Hossain
Vill.
: Dhohakhula
P.O.
: Bagherpara
P.S.
: Bagherpara
Dist.
: Jessore
Country: Bangladesh