Download as DOC-file for conventional and Realtime PCR

Ready to use PCR kits for Influenza viruses (particularly H1N1) and related micro
organisms*:
Here are the best tools to do the research:
A). Swine flu Virus: Genekam developed her own assays for molecular detecting
of various kinds of H1N1 strains in gel agarose, we are also developing assays for real time
PCR. If one understands the H1N1 strains of influenza viruses correctly and keep in mind
recent outbreak strains , these H1N1 strains can be divided in 3 classes: 1) H1N1 strain of
recent outbreak (so called novel strain of H1N1 of swine origin) 2) seasonal H1N1 strain (so
called novel strain of H1N1 of human origin) 3. H1N1 strain of swine circulating in swine
populations. There are a number of research laboratories, which are focusing and making
publications for detecting two strains i.e. Class 1 and 2, therefore Genekam has decided to
bring one kit to genotype them along with bringing a number of assays to detect H1N1 strain of
recent outbreak of swine flu. Such kits are needed to understand the distribution of such
viruses properly and may help to generate more accurate preventative measurements along
with perhaps providing better understanding to find better therapies, hence these kits can be
used in research work. Genekam has developed the solutions, which are highly specific to
detect only the recently outbreak H1N1 virus like California, Mexico, New York, Texas,
Canada, Hamburg, Regensburg, Italy, Norway, Beijing, Shanghai, South Australia, Louisiana,
Victoria, Chile, Thailand, Korea, Ankara, Philippines, Bayer, Catonia and other strains. We
have developed the assays, which can detect N1 part of influenza A viruses like H1N1, H5N1,
H6N1, H7N1 etc. Genekam kit needs only isolated RNA to conduct the assay and this contains
components like PCR master mix, polymerase, positive control, negative control, primers,
buffers, loading dye, generuler etc. In order to develop a highly specific assay for recent H1N1
virus, one must understand the influenza viruses very deeply along the good knowledge of
software tools, therefore we have decided to offer the following ready to use PCR kits based
on gel agarose:
1. Kit K736 Ready to use PCR kit to detect H1N1 of swine origin of recent outbreak (single
check) 100 reactions Euro 799,This kit is in position to detect the H1N1 of swine origin of recent outbreak in samples. One can
see clear beautiful bands in the gel agarose. This assay detect only H1N1 strains and there
should be no cross reactions with H3N2, H5N1; H5N3, H7N7 strains and other strains. This is
not available in literature. This is Genekam´s own development.
2. Kit K746 Ready to use PCR kit to detect H1N1 of swine origin circulating in swine (single
check) 100 reactions Euro 799,This assay should be used to detect H1N1 of swine origin in the samples of animals like
pigs. This assay cannot detect recently outbreak H1N1 strain (so called Novel H1N1 of swine
origin).
3. Kit K794 Ready to use PCR kit to detect the H1N1 of human origin (single check)
reactions Euro 799,-
100
This assay can detect H1N1 of human origin (also known as seasonal H1N1 or novel H1N1 of
human origin) in the cell cultures, nasal or other samples. This does not detect the recently
outbreak H1N1 virus.
4. Kit K745 Fluhunter 3X: Ready to use PCR kit to detect and
genotype three different kinds of H1N1 (recently outbreak swine flu
virus or Novel H1N1 of swine origin, seasonal H1N1 or Novel virus of
human origin and H1N1 virus of swine origin) 100 reactions Euro
1499,-
5. Kit K414 Ready to use PCR kit to detect the recently outbreak virus of swine flu
H1N1 (double check) 100 reactions Euro 1199.6. Kit K415 Ready to use PCR kit to detect the recently outbreak virus of swine flu H1N1
(triple check) 100 reactions Euro 1499,7. PCR Positive control for recently outbreak H1N1 D0069 (as cDNA)
8. Kit K413
599,-
Ready to use PCR kit to detect N1 part of H1N1 viruses
call for price
100 reactions
Euro
This assay can help to type of N1 part of various influenza A viruses like H1N1, H5N1, H6N1
etc.
9. Kit K712 Ready to use PCR kit to differentiate between recently outbreak strain of H1N1
and seasonal H1N1 of human origin 100 reactions Euro 899,-
This is two in one assay, which can detect the seasonal as well as swine flu virus of
recent outbreak of human origin. This assay can detect the different strains of recent
outbreak like different strains of Mexico, California, New York, Italy, Regensburg,
Hamburg, Canada, Texas, Bayer, Rio de Janero etc.
10. Influenza H3N2: Ready to use kit to detect H3N 2 infection
122d 100 Reactions Euro 999,This is circulating in human population and swine population.
Kit
11. Kit K416 Ready to use PCR kit to detect recent outbreak H1N1 of human
origin and reconfirming the N1 part of H1 100 reactions Euro 899,This assay can be used to detect the H1N1 strain of recent outbreak swine flu
virus and reconfirming the N1 part of this virus. This is two in one assay. This
assay is Genekam´s own development.
B). Genekam developed new solutions like our real time Fluhunter FR060 kit for Influenza A kit,
which can detect specially H1N1 strains (also swine flu strains of Mexico, California,
Regensburg, Hamburg, Rio de Janeiro, New York, Texas, Canada etc), H3N2 strains along with
other strains of Influenza A like H5N1, H9 and others. 2. Similarly our old kits 203A (sold for
last 4 years on the market) for Influenza A can also detect recent outbreak strains of
H1N1 along with other influenza strains. 3. Our kits can be used to write the PhD thesis for human
and veterinary medicine (including dental medicine). They can be used to compare the quality of
the laboratories own assays. These kits offer excellent chance to do more research in the field of
influenza viruses.
C).
Ready to use PCR kits for influenza virus (conventional as well as real time PCR) :
1. Ready to use PCR Kits for Influenza A , H1, H3, (H5N1) , H7 and H9 :
1.1 Fluhunter (special version) Kit: 333a ready to use kit (H5N1) one step kit
Reactions
Euro 499,-
100
1.2 Fluhunter (special version) kit: 333b ready to use kit (H5N1) one step kit
genesequencings Call
100 Reactions with 5
1.3 Fluhunter (special version) Kit: 333c ready to use kit (H7) one step kit
499,-
100 Reactions Euro
(This kit can be used to detect H7 virus in horses. H7 causes equine influenza in horse. All Genekam
H7 kits can be used to detect equine influenza also.)
1.4 Fluhunter (special version) Kit: 333d ready to use kit (H9) one step kit
499,-
100 Reactions Euro
2. Professional ready to use PCR kits for H5N1 and H9: Our kit H9 can detect H9N2, which is a major
poultry virus.
2.1 Ready to use PCR kit for H5N1 and H9 Kit 122a
100 Reactions Euro 899,-
2.2 Ready to use PCR kit for H5N1 and H9 kit 122b
100 Reactions+2 genesequencings Euro 999,-
2.3 Ready to use PCR kit for H5N1 and H9 kit 122c
1499,-
100 Reactions+15 genesequencings Euro
Kit 122a can be used for birds, mammals like cats as well as human beings. This is right
choice for professional use.
3. Professional ready to use PCR kit: In this kit, one have to test the H5N1 twice, one can reconfirm
the results. It will take 24 hours to give you the results.
3.1 Ready to use PCR kit (H5N1) Kit 123a
3. 2 Ready to use PCR kit (H5N1) Kit 123b
100 Reactions
Euro 899,-
100 Reactions + 2 genesequencings Euro 999 -
4. Most Professional kits: Ready to use PCR kit for H5N1, H9 as well as H7: Here you can test all
strains of avian influenza viruses, which come in question.
4.1 Fluhunter: Ready to use PCR kit (H5N1, H9 and H7)
Kit127a
4.2 Fluhunter: Ready to use PCR kit (H5N1, H9 and H7)
genesequencings Euro 1 599,-
Kit 127b
100 Reactions
Euro 999,-
100 Reactions+15
5. Influenza A: ready to use PCR kit for Influenza A one step Kit 203a
Euro 599,-
100 Reactions
This kit can be used to see the influenza A infection in human, birds, horses, dogs,
pigs and other animals samples.
6. Influenza B: ready to use PCR kit for Influenza B
Kit 400
100 Reactions
Euro 799,-
7. Influenza H1N1 and H3N2:
7.1. Influenza H1N1 (seasonal and recent outbreak strains) : Ready to use kit to
detect H1N1 infection Kit 712 100 Reactions Euro 899,This is two in one assay, which can detect the seasonal as well as swine flu virus of recent
outbreak of human origin. This assay can detect the different strains of recent outbreak
like different strains of Mexico, California, New York, Italy, Regensburg,
Hamburg, Canada, Texas, Bayer, Rio de Janeiro etc.
7.2 . Influenza H3N2: Ready to use kit to detect H3N 2 infection
122d 100 Reactions Euro 999,This is circulating in human population.
Kit
8. Influenza H5N1 and H7: Ready to use kit (H5 and H7)
Kit 177
100 Reactions
Euro 699,-
9. Ready to use real time PCR kit for Influenza A Kit F005 100 Reactions Euro 899,They are with fluorogenic primers. This can be used on ABI and compatible machines like
Stratagene, Cepheid, Rotorgene , Techne etc.
10. Ready to use real time kit for Influenza B Kit F0011 100 Reactions Euro 899,They are with fluorogenic primers. This can be used on ABI and compatible machines like Techne,
Stratagene, Cepheid, Rotorgene etc.
11. Ready to use real time Influenza H5N1 Kit F006 100 Reactions Euro 899,They are with fluorogenic primers. This can be used on ABI and compatible machines like
Techne, Stratagene, Cepheid, Rotor-Gene etc.
12. Ready to use real time Influenza H9
Kit F055 100 Reactions
Euro 799,-
13. Ready to use real time Avian influenza H5N1 one step Kit F029 100 Reactions
Euro 699,-
14. Ready to use real time Influenza A Kit F060 100 Reactions Euro 699,They are with fluorogenic primers. This can be used on ABI and compatible
machines like Techne, Stratagene, Cepheid, Rotor-Gene etc
This kit is designed specially to detect the H1N1 as well as H3N2 strains
(including seasonal as well as the strains of swine flu H1N1 like Mexico,
California, New York, Italy, Regensburg, Canada etc) and other strains like H5N1,
H9 etc.
15. Ready to use real time Influenza H3
Kit F059
100 Reactions
Euro 699,-
16. Ready to use real time Influenza H1
Kit F058
100 Reactions
Euro 699,-
17. K413 Ready to use PCR kit for N1 of Influenza A Kit K413
100 Reactions Euro 599,-
d). Related micro organism causing similar diseases like avian influenza viruses in human
and birds for differential diagnosis:
1. New castle disease virus (NDV): Ready to use PCR kit for NDV
699,-. This is important for poultry.
Kit 049
100 Reactions
2. Human respiratory syncytial virus: Ready to use kit for RSV
Euro 999,-
Kit 710A
3. Mycobacterium avium sp. paratuberculosis
K032 Euro 599,-
4. Mycobacterium tuberculosis
Kit 071
100 reactions
100 Reactions
Euro 499,-
5. Mycobacterium complex (to differentiate between TB and Non TB (MAP))
Reactions Euro 499,6. Chlamydia pneumoniae Kit K015
100 Reactions
7. Mycoplasma pneumoniae
KitK011
8. Human adeno virus
Kit 076
Euro 399,-
100 Reactions
100 Reactions
100 Reactions
Euro 399,Euro 399,-
Kit K038
100
Euro
9. Infectious bronchitis virus
This is important for poultry.
Kit K113
100 Reactions
10. Infectious Bursal disease virus (Gumboro virus)
This is an important viral disease for poultry.
11. Mycoplasma gallisepticum kit K006
(Real time PCR kit is also available.)
12. Mycoplasma synoviae
Kit K007
Euro 699,-
Kit K121
100 Reactions
100 Reactions
100 Reactions
Euro 699,-
Euro 199,-
Euro 199,-
13. Positive controls for Influenza viruses (H5, H7 and H9): visit our website www.genekam.de.
14. Human Parainfluenza 1, 2 and 3
K062
100 Reactions
Euro 1199,-
E) Genekam offers other kits for checking the respiratory micro organisms :
Streptococcus, Staphyloccus, Chlamydia psittaci and other viruses. We carry 350 ready to use real
time and conventional PCR kits, please visit our website www.genekam.de to see all.
*only for research use.
With best regards,
Genekam Biotechnology AG
Dammstr. 31 - 33
47119 Duisburg
Germany
Tel: +49-203-555858-31, -32
Fax: +49-203-358299
Email: [email protected]
Internet: www.genekam.de