Download Lentiviral (HIV) Vector Post Exposure Procedures

Lentiviral (HIV) Vector Post Exposure Procedures
Sansum Occupational Health (805) 898 – 3311
UCSB Workers Compensation (805) 893 – 4440
[email protected] (805) 893 – 8894
Information for Researchers
Definition of Exposure
1. Broken skin, eye or mucosal membrane exposure to the virus,
2. Parenteral inoculation by a syringe needle or other contaminated sharps (needle stick),
3. Ingestion of liquid suspension of an infected material or by contaminated hand to mouth exposure,
4. Inhalation of infectious aerosols.
Post Exposure Procedures:
1. Remove exposed personal protective equipment, taking care to avoid contact with contaminated
materials
2. Inform others in the area about any biohazardous materials out of containment to prevent further
exposure. If possible, contain with absorbent pads, decontaminate with bleach, and/or seal off the
area. All exposed individuals should leave the area.
3. Immediately wash/clean the area of exposure/cut/puncture with soap thoroughly for 5 mins. For
mucous membrane exposure, flush the exposed membrane with the eye wash continuously for 5
minutes.
4. After washing, immediately proceed to receive consultation regarding medical treatment.
Post exposure prophylaxis must be initiated within 2 hours of exposure if you decide to receive it.
5. Take these instructions with you.
During work hours, report to Sansum
Occupational Health Clinic
 (805) 898 - 3311
 Open 8am - 5pm Mon – Fri
 Located at 101 South Patterson
 Non-employee students, please specify
your status and indicate this was an
occupational-like laboratory exposure
Page 1 of 3
Reviewed and approved by Dr. Mark Musicant, MD; revision date: 9/1/2016
After hours, weekends and holidays, report to
Sansum Urgent Care
 (805) 563 - 6110
 8am – 7pm Monday – Friday
9am – 5pm Saturdays
9am – 3pm Sundays
 Located at 215 Pesetas Lane
6. Inform the health care provider about the
exposure and give them these instructions.
7. After medical care, ensure that the incident
is immediately reported to
a. your faculty principal investigator,
b. UCSB Workers Compensation, (805) 893 – 4440, and
c. the campus biosafety officer, (805) 893 - 8894.
Information for Healthcare Providers



Urgent care is required
The recombinant virus used in this research protocol is based on the human immunodeficiency
virus. It has been genetically modified to remove genetic elements required for replication to
create “3rd or 4th generation lentiviral vectors,” but it is still capable of infecting human cells. The
virus still retains the potential risk to cause insertional mutagenesis in exposed cells, although
this risk is believed to be low.
Treatment with agents that interfere with the early life cycle, i.e., reverse transcription and
integration, is recommended. Sansum Occupational Medicine will adhere to the CDC
recommendations for the standard three drug protocol for a treatment course of five (5) days
after exposure to replication-incompetent 3rd and 4th generation lentiviral vectors.
Drug
Raltegravir (Isentress)
Dose
1 x 400 mg tb BID
w/o regard to food
Duration
5 days
Notes
Within 2 hours of
exposure
TRUVADA
(emtricitabine and
tenofovir disoproxil
fumarate)
1 x 200mg cap QD
w/o regard to food
5 days
Within 2 hours of
exposure
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Reviewed and approved by Dr. Mark Musicant, MD; revision date: 9/1/2016
Additional information for healthcare providers on the lentiviral vector:
This section is intended to be completed by the faculty principal investigator.
What cell types is the viral vector capable of infecting?
What type of effect is the virus designed to have on the target cells?
What are the activities of the gene products inserted in the virus?
Which other potentially infectious materials may be reasonably expected to be present along with the
viral vector?
Page 3 of 3
Reviewed and approved by Dr. Mark Musicant, MD; revision date: 9/1/2016