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Midterm (60 minutes) Closed Book
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Environmental microbiology:
a) The study of microorganisms that exist in artificial environments such as bioreactors.
b) Is the study of the composition and physiology of microbial communities in the environment
c) Both a and b
The three domain system
a) Bacteria, Archaea, Protozoa
b) Bacteria, Virus, Eucarya
c) Bacteria, Archaea, Eucaryota
Eukaryote
a) Has two or more chromosomes
b) Has no membrane bound organelles
c) Both a and B
Prokaryotes
a) Characterized by lack of nuclear membrane and low level of differentiation
b) Which have a nuclear membrane and have more highly differentiated cells
c) Which have some membrane bound organelles
Rod shaped bacteria are called
a) Cocci
b) Vibrio
c) Bacilli
Gram positive cell wall- bacteria characterized by
a) Larger periplasmic space
b) Peptidoglycan and techoi acids
c) Outer membrane contains lipopolysaccharides
Transduction
a) Bacterial cells exchange DNA by using a pilus
b) Is the uptake of free DNA released from a dead or dying cell by a recipient cell.
c) Is the transfer of host bacterial DNA to a recipient bacterium via a bacteriophage
Proteins to Amino Acids, Starch to Glucose
a) Anabolism process
b) Catabolism process
c) Both a and b
Obligate anerobes
a) Microorganisms that can survive in the presence and absence of oxygen
Midterm (60 minutes) Closed Book
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b) Microorganisms that can not survive in the presence of oxygen
c) Microorganisms that use CO2 as a carbon source.
Under anaerobic condition
a) An alternative TEA such as NO3-, Fe3+, SO4 2- or CO2 is used
b) The TEA is oxygen
c) Either O2 or NO3
Respiration in bacteria represents the reaction
a) 6H2O + 6CO2 + light  C6H12 O6 + 6O2
b) NH4  NO2 to NO3
c) C6H12 O6 + 6O2 6H2O + 6CO2 + 38 ATP
Nitrobacter, Nitrosomonas
a) Ammonium oxidation
b) Nitrogen fixation
c) Nitrate removal from wastewater
The 18s rRNA, gene is being used for
a) Bacteria identification
b) Fungal identification
c) Virus identification
Protozoa is
a) Procaryotic
b) Heterotrophic
c) Both a and b
Disposal of rich phosphorus wastewater to the aquatic system enhance the growth of
a) Bacteria
b) Protozoa
c) Algae
Viruses consist of
a) DNA and RNA, and a protective coat of protein molecules
b) DNA or RNA, and a protective coat of protein molecules
c) Only RNA and a protective coat of protein molecules
Bacteriophages attack bacteria - Lytic Cycle
a) A parasitic type of partnership with the cell
b) Destroying the host cell during reproduction
c) Both a and b
Midterm (60 minutes) Closed Book
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Phage and protozoa have one common characteristic
a) Predators of prokaryotes in marine water.
b) Controlling marine cyanobacteria
c) Control bacteria population by induction of the lytic cycle
A VIROID consists of
a) Only of Nucleic Acid (RNA)
b) Only infectious proteins
c) DNA or RNA without proteins
Oligotroph (k-Strategists)
a) Organisms tend to be found in environments which are rich in nutrients, particularly carbon
b) Organism that can live in a very low carbon concentration, less than one part per million
c) Organism that exhibit high rates of metabolism and perhaps exponential growth for short periods
To measure the BOD, the sample should be incubated for 5 days at
a) 40 oC
b) 20 oC
c) From 25 to 30 oC
Wastewater sample is diluted 30 times in BOD bottle. The initial DO of the sample is 9 mg/L.
The DO is 2 mg/L after 5 days. What is the 5-day BOD?
a) 165
b) 210
c) 300
BOD is indicator for
a) Organic pollution in wastewater
b) Microbial growth in wastewater
c) Nitrate pollution in wastewater
COD in wastewater is mostly
a) Lower than BOD
b) Equals BOD
c) Higher than BOD
Nitrification - conversion of
a) Ammonia and ammonium to nitrate
b) Nitrate to nitrogen
c) Ammonia to ammonium
Denitrification- conversion of
Midterm (60 minutes) Closed Book
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a) Ammonia to ammonium
b) Nitrate to nitrogen gas
c) Both a and b
Heterotrophic microorganisms
a) Microorganisms that rely only on the light for energy
b) Microorganisms that use organic matter as a source of carbon
c) Microorganisms that extract energy from chemical reactions
Aerobic- chemo- heterotrophic
a) O2 from organic matter, energy by oxidation reduction and carbon from CO2
b) dissolved O2, energy by light and carbon from organic matter
c) dissolved O2, energy by oxidation reduction and carbon from organic matter.
No lag phase when
a) Microbes inoculated into fresh medium
b) Exponentially growing culture inoculated into same media, same growth conditions
c) Cells transferred from poor medium to rich culture medium
Batch culture
a) The contents of the culture are mixed completely
b) During reaction no flow is allowed in or out of the culture
c) Both a and b