Download Transformation Quiz True or False: During the transformation

Transformation Quiz
1. True or False: During the transformation experiment, growth on the – pGLO LB/amp plate should have been
much heavier than growth on the – pGLO LB plate.
A) True
B) False
2. After attempting to transform a strain of E. coli with a plasmid that carries kanamycin (kan) resistance, which
of the following nutrient plates (NP) should the strain grow on to confirm a successful transformation?
A) NP
B) NP/ Kan
C) NP/AMP
d. NP/AMP/ARA
3. What was the purpose of adding CaCl2 and performing a heat shock in the experiment?
A) This activated the pGLO plasmid
B) This made holes in the cell wall and membrane of E. coli
C) This killed cells sensitive to the antibiotic ampicillin
D) This made the cells fluoresce green
4. What genes (superpowers) were transformed into your E. coli cells?
A) Ampicillin resistance, green fluorescence, and the ability to metabolize arabinose
B) Penicillin resistance and the ability to metabolize arabinose
C) Ampicillin resistance and the ability to fluoresce green
D) The ability to produce toxins and ampicillin resistance
5. What would be the expected results if you have a plate labeled + pGLO LB?
A) White colonies
B) No growth
B) Green glowing colonies
D) White and green glowing colonies
6. How could you tell if the E. coli were able to metabolize arabinose in your experiment?
A) The cells gained resistance to ampicillin
B) The cells produced white colonies
C) The cells were able to grow on the LB/AMP plate
D) The cells were able to glow green
7. What occurs during the process of transformation?
A) A replicating virus inserts new DNA into a bacteria cell
B) A pilus connects two neighboring bacterial cells and allows them to transfer DNA
C) Naked DNA in the environment is taken up by a bacteria cell
D) Cells are able to construct capsules that pass DNA
8. Which of the following plates would you expect to find glowing green colonies?
A) – pGLO LB
C) + pGLO LB/AMP
B) – pGLO LB/AMP
D) + pGLO LB/AMP/ARA
9. What directly caused the E. coli to glow under UV light?
A) The pGLO plasmid
C) Beta-lactamase
B) Production of green fluorescent proteins
D) Ampicillin resistance gene
10. What two factors need to be present in the bacteria’s environment in order for the cells to turn green?
A) Arabinose and UV light
C) UV light and Ampicillin
B) Ampicillin and Arabinose
D) LB and Arabinose
11. Transformation is a special kind of horizontal gene transfer.
A) True
B) False
Transformation Quiz
Match the results to the specified plate. (Answers may be used more than once)
A) Growth (white colonies)
C) No growth
B) Growth (green glowing colonies)
D) White and green glowing colonies
12. ____ - pGLO LB
13. ____ - pGLO LB/AMP
14. ____ + pGLO LB/AMP
15. ____ + pGLO LB/AMP/ARA