Download Enzyme Action—Bubbles, Bubbles Everywhere

Technology-Connected Lesson Plan
Title:
Grade Levels:
Curriculum Areas:
Measurable Objectives:
LA Content Standards:
(SI GLEs: 1, 4, 9, 10; LS
GLE: 3)
Technology Standards:
Enzyme Action—Bubbles, Bubbles Everywhere
Grades 10th – 12th
 Biology
 TLW complete all stages of scientific investigation to determine enzyme
action on various specimens.
 TLW use a digital camera/document camera to record the stages of
enzyme action on various specimens to be used to complete the lab
report.
*SI 1-Write a testable question or hypothesis when given a topic (SI-H-A1)
*SI 4- Conduct an investigation that includes multiple trials and record,
organize, and display data appropriately (SI-H-A2)
*SI 9- Write and defend a conclusion based on logical analysis of
experimental data (SI-H-A6) (SI-H-A2)
*SI 10- Given a description of an experiment, identify appropriate safety
measures (SI-H-A7)
*LS 3- Investigate and describe the role of enzymes in the function of a
cell (LS-H-A1)
1. Technology Communication Tools (Communication Foundation
Skill)
with peers, experts and other audiences.
present information and ideas effectively to multiple audiences.
3. Technology Productivity Tools (Resource Access and Utilization
Foundation Skill)
productivity, and promote creativity.
tively in developing
technology-rich, authentic, student-centered products.
4. Technology Research Tools (Linking and Generating Knowledge
Foundation Skill)
5. Social, Ethical, and Human Issues (Citizenship Foundation Skill)
support lifelong learning, collaboration, personal pursuits, and productivity.
6. Basic Operations and Concepts
anding of the nature and
operation of technology systems.
Technology Connection:
 Computer w/Digital Camera or Document Camera
 Smart Notebook, Microsoft PowerPoint, Thinking Maps
Software, or Inspiration
 Printer
 Resources for lab activities on enzymes or use the lab activity
from the textbook:
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http://www.accessexcellence.org/AE/AEC/AEF/1996/crumlish_enzyme.php
http://www.blackgold.ab.ca/microtest/Biology20Labs/enzyme_catalase.pdf
http://www.sciencetechnologyaction.com/lessons2.php?studyid=56
Procedures:
 Introduction: Using the chosen lab activity to demonstrate enzyme
activity on various specimens (see Comp. Curriculum Unit 1 Activity 7
located below.) set up the lab specimens in such a way that a
document camera or digital camera may be used to record the
progress of the experiment.
 Take several photos of the specimens as the experiment progresses
making sure to add a paper label in the shot that describes the
specimen and time of the picture.
 Once students have completed the actual experiment load the images
onto the computer and store them in a folder labeled “enzyme photos”
for easy retrieval.
 To complete the lab activity, have students create a flow map using
Smart Notebook, Inspiration, Thinking Maps software, or Microsoft
PowerPoint to trace the time lapsed events of enzyme action on the
different specimens. Students will have to add the images in the
correct order by inserting the image files one at a time into the
program. Once students have created their flow maps, have them add
descriptions under each stage of the map to describe exactly what is
occurring in that stage.
 Have students print out the completed flow maps and turn them in
with the rest of the lab report.
 Students should be creative and make their maps unique to promote
better understanding of the concept.
 Explain to the students to use the maps to completely map out the
order of events of the experiment.
Materials:
 Appropriate lab materials (see comp. curriculum Unit 1 Activity 7
located below.)
 Printed flow map and lab report
 Aimee Bancroft
 C.M. Fagan
Assessment:
Teacher’s Name:
School:
Activity 7: Enzyme Action—Bubbles, Bubbles Everywhere
(SI GLEs: 1, 4, 9, 10; LS GLE: 3)
Materials List: small disposable plastic cups or test tubes, hydrogen peroxide, distilled water, small
pieces of beef liver or raw, white potato, graduated cylinders, computer with Internet access (if
available), Experimental Design Assessment Rubric BLM (one for each group of students) Ask
students which cellular organelle is responsible for storing enzymes (Lysosomes). Conduct this
activity after instruction, with illustrations, that explains to students how enzymes act as catalysts;
they enable or speed up reactions without being altered themselves. In this activity, have students
work in groups to make observations on the action of the enzyme catalase on hydrogen peroxide
(H2O2). Provide students with the lab directions or an overview of the investigation and have them
work in groups to write a testable hypothesis for the investigation, write questions to be answered,
design their own data table for their lab report, and identify appropriate safety measures for this
investigation. Before the investigation, assess each group’s design using the Experimental Design
Assessment Rubric and make revisions where needed. Using either a small piece of beef liver or a
piece of raw potato, have students place a small piece of the liver or potato into approximately 10
2
mL of distilled water, observe any reaction, and record the results. Have students repeat this three
times and observe each time, recording observations. Using the same piece of liver or potato,
students will then place the substance into 10 mL of hydrogen peroxide (H2O2), remove after two
minutes, and observe and record the reaction. Have students repeat the investigation three times
(three trials) with the same hydrogen peroxide as used in the first reaction, record observations in a
data table, write an analysis of what they observed, write a conclusion relating to the hypothesis,
and record possible roles that enzymes may play in cells. To conclude the activity, conduct a full
class discussion on the functions of enzymes in a cell. Use teacher created questions to elicit correct
examples and responses.
Louisiana Comprehensive Curriculum, Revised 2008
If technology is available, students may visit the following web site for good explanations of enzyme
action: http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/E/Enzymes.html
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