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Replication, Transcription, Translation
Replication, Transcription, Translation

... carrying each of the four bases move into place by forming hydrogen bonds with the bases exposed on the DNA template strand. DNA polymerase catalyzes bond formation between the 5’ phosphate group of the arriving nucleoside triphosphate and the 3’ —OH at the end of the growing polynucleotide strand. ...
Teacher`s Notes - University of California, Irvine
Teacher`s Notes - University of California, Irvine

... smaller fragments. Thus, larger fragments will move slower than smaller fragments. This allows separation of all different sizes of DNA fragments. 10 min. ...
DNA & RNA
DNA & RNA

... • Hydrogen bonds hold the two strands together but only formed between A&T and G&C • This is called base pairing ...
Ligation and Transformation
Ligation and Transformation

... colonies floresce green (+pGLO) ...
AP genetic technology
AP genetic technology

... – Discovered bacteria have an enzyme that chops up viral DNA • Restriction enzymes cut DNA at a specific sequence • Number of cuts made in DNA will depend on number of times the “target” sequence occurs ...
AP Biology Study Guide Key Chapter 18
AP Biology Study Guide Key Chapter 18

... d. They are infectious proteins that may convert brain proteins into misfolded forms. 11. The herpesvirus a. acts as a provirus when its DNA becomes incorporated into the host cell’s genome 12. The replication of the genome of an RNA virus uses b. RNA replicating enzymes coded for by viral genes 13. ...
to view fulltext PDF - Indian Academy of Sciences
to view fulltext PDF - Indian Academy of Sciences

... The elucidation of the structure of DNA and the realization that DNA provides an information template for protein synthesis has been the corner stone of modern biological research [1]. DNA serves as an information template for gene expression, while being a flexible polymer chain. A specific DNA seq ...
RC 2 Student Sheet
RC 2 Student Sheet

... Almost all the cells in your body were produced by mitosis. The only exceptions are the gametes – sperm or egg cells – which are produced by a different type of cell division called meiosis. During fertilization the sperm and egg unite to form a single cell called the zygote which contains all the c ...
Microbial Genetics
Microbial Genetics

...  5’ – phosphate group  3’ – hydroxyl group ...
Slide 1
Slide 1

... The Genetic Code • Proteins are made by joining amino acids into long chains called polypeptides or proteins. • The properties of proteins are determined by the order in which different amino acids are joined together to produce polypeptides • The language of mRNA instructions are in the form of co ...
Efficient, closed-tube DNA extraction using prepGEM® Bacteria
Efficient, closed-tube DNA extraction using prepGEM® Bacteria

Recombinant DNA Technology
Recombinant DNA Technology

... BAC is a DNA construct, based on a fertility plasmid (or F-plasmid), used for transforming and cloning in bacteria, usually E. coli. F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division. BAC's usual insert si ...
File
File

... Taq DNA polymerase is a temperature resistant enzyme which builds DNA strands. Taq was isolated from the bacterium Thermus aquaticus, which normally lives in hot springs in temperatures around 100° C. Taq is stable under the extreme temperature conditions of PCR. ...
anth-260-midterm-review-sheet-2016
anth-260-midterm-review-sheet-2016

... • According to Boyd and Silk, stabilizing selection tends to prevent traits of organisms changing over time. a. True b. False • All of the following are true of the relationship between DNA and proteins EXCEPT: a. a sequence of three DNA base-pairs codes for one amino acid b. a single codon codes fo ...
DNA - Ellis Benjamin
DNA - Ellis Benjamin

... • Gene therapy – replacing faulty genes • Block gene expression to silence harmful gene or study gene function – Antisense RNA, gene knockouts ...
Aim
Aim

... Genetic diversity exists in a population of individuals of the same species. This is usually in terms of differences in nucleotide sequences termed alleles of the gene. Some of these alleles may be transcribed (transform genetic information from DNA into RNA) and then translated (transform genetic i ...
Mutations - Hicksville Public Schools
Mutations - Hicksville Public Schools

Build whatever you want - Hicksville Public Schools / Homepage
Build whatever you want - Hicksville Public Schools / Homepage

... the nucleus 3. Translation: tRNA reads mRNA codons (3 bases) and brings the correct amino acid to the ribosome 4. Sugar: DNA= deoxribose, RNA= ribose Bases: DNA has T and RNA has U DNA: double stranded, RNA: single stranded 5. UGG CAG UGC Try Glu Cys ...
PCR applications in diagnosis of parasitic diseases
PCR applications in diagnosis of parasitic diseases

...  RT_PCR quantitative measurement of RNA or DNA:  there will be detection of “amplification associated florescence” in each cycle. It is used in diagnosis of many conditions other than parasitological infections. ...
Chapter 9 Genetics Chromosome Genes • DNA RNA Protein Flow of
Chapter 9 Genetics Chromosome Genes • DNA RNA Protein Flow of

... F+ and F- - the former are the donor cells and the latter are the recipient cells. The donor cells have an F plasmid – sex pili and DNA Transfer. Conjugation in this case is a transfer of the F plasmid from the donor to the recipient. ...
Biology Lecture 2 – Genes
Biology Lecture 2 – Genes

... • Prophase I: homologous chromosomes line up next to each other to form tetrads (XX), crossing over or genetic recombination occurs • Metaphase I: XX lines up at equator • Anaphase I: two X’s split, travel towards opposite ends of cell • Telophase I: nuclear membrane may reform • Cytokinesis: primar ...
Slide 1
Slide 1

... – Insight into evolutionary process? ...
PDF
PDF

... In molecular terms, epigenetics is concerned with the study of chromatin organisation in the eukaryotic nucleus, with respect to the regulation of gene expression during the development of plants, fungi, insects and animals through embryogenesis to adulthood, and in disease states. A major intellect ...
Lab - TeacherWeb
Lab - TeacherWeb

... Scientists now have an understanding of how chromosomes replicate prior to cell division and transfer their genetic information to every new cell. Scientists also recognize how the DNA molecule "stores" the hereditary information in the genes, and how this information is used to synthesize proteins ...
BIOL 1406 - Ch. 16-18 Review
BIOL 1406 - Ch. 16-18 Review

... An mRNA transcript receives a __________ at 5' end and a ________ at the 3' during RNA processing before it is transported out in the cytoplasm. A. a repeat nucleotide, a unique sequence B. codon, anticodon C. piece of RNA, piece of DNA D. G-3-P cap, poly A tail E. introns, exons ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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