Basic principles of DT40
... • Good model for genome stability in mammals • Complementation by human genes • Good database ...
... • Good model for genome stability in mammals • Complementation by human genes • Good database ...
Document
... 2. Cool DNA: complementary strands find each other & anneal • Hybridize: don't have to be the same strands ...
... 2. Cool DNA: complementary strands find each other & anneal • Hybridize: don't have to be the same strands ...
1000 - s3.amazonaws.com
... Two heterozygous guinea pigs cross. How many offspring will have short ...
... Two heterozygous guinea pigs cross. How many offspring will have short ...
Name __________ Introduction: People with diabetes require extra
... DNA. These plasmids frequently carry genes for antibiotic resistance. They are also easily separated from bacteria and easily reabsorbed into bacteria. Because of these properties, scientists use plasmids as a vector (carrier) for the insulin gene. Once the plasmids (includes DNA for insulin) are ab ...
... DNA. These plasmids frequently carry genes for antibiotic resistance. They are also easily separated from bacteria and easily reabsorbed into bacteria. Because of these properties, scientists use plasmids as a vector (carrier) for the insulin gene. Once the plasmids (includes DNA for insulin) are ab ...
Bacterial Nucleic Acids
... • DNA---Chromosomes---Genes • Genes –small sequences of DNA • Carries all information for –development and function • Their information is used to make protein with the help of RNA through Transcription...Translation. • The DNA double helix is stabilized by hydrogen bonds between the bases attached ...
... • DNA---Chromosomes---Genes • Genes –small sequences of DNA • Carries all information for –development and function • Their information is used to make protein with the help of RNA through Transcription...Translation. • The DNA double helix is stabilized by hydrogen bonds between the bases attached ...
BIOTECHNOLOGY
... missing the -OH group on carbon 3 and therefore inhibit the process of replication. Every time one is added, the process stops and only small sequences are created. ...
... missing the -OH group on carbon 3 and therefore inhibit the process of replication. Every time one is added, the process stops and only small sequences are created. ...
DNA Replication - susanpittinaro
... • DNA composition: “Chargaff’s rules” • Varies from species to species • All 4 bases not in equal quantity • Bases present in characteristic ratios ...
... • DNA composition: “Chargaff’s rules” • Varies from species to species • All 4 bases not in equal quantity • Bases present in characteristic ratios ...
PCR and Its Applications
... What is PCR? : The “Reaction” Components 1) Target DNA - contains the sequence to be amplified. 2) Pair of Primers - oligonucleotides that define the sequence to be amplified. 3) dNTPs - deoxynucleotidetriphosphates: DNA building blocks. 4) Thermostable DNA Polymerase - enzyme that catalyzes the re ...
... What is PCR? : The “Reaction” Components 1) Target DNA - contains the sequence to be amplified. 2) Pair of Primers - oligonucleotides that define the sequence to be amplified. 3) dNTPs - deoxynucleotidetriphosphates: DNA building blocks. 4) Thermostable DNA Polymerase - enzyme that catalyzes the re ...
DNA replication
... “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material.” ...
... “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material.” ...
Final Review
... DNA and RNA molecules have structural similarities and differences that define function. ● Both have three components — sugar, phosphate and a nitrogenous base — which form nucleotide units that are connected by covalent bonds to form a linear molecule with 3' and 5' ends, with the nitrogenous bases ...
... DNA and RNA molecules have structural similarities and differences that define function. ● Both have three components — sugar, phosphate and a nitrogenous base — which form nucleotide units that are connected by covalent bonds to form a linear molecule with 3' and 5' ends, with the nitrogenous bases ...
Characteristics of Living Things (Essay
... chromosomes? What are alleles? In comparing a pair of homologous chromosomes - chromosome #1 in Humans for example - is it likely for this pair of chromosomes to contain identical alleles at each and every gene loci along the entire chromosome? Please explain your reasoning carefully! Explain the pr ...
... chromosomes? What are alleles? In comparing a pair of homologous chromosomes - chromosome #1 in Humans for example - is it likely for this pair of chromosomes to contain identical alleles at each and every gene loci along the entire chromosome? Please explain your reasoning carefully! Explain the pr ...
DNA Tech
... Scientists use several techniques to manipulate DNA (cloning = copying genes, transferring genes between organisms, etc.) DNA must first be extracted and precisely cut so that it can be studied. Restriction enzymes (or molecular scissors) cut DNA at a certain nucleotide sequence called a restriction ...
... Scientists use several techniques to manipulate DNA (cloning = copying genes, transferring genes between organisms, etc.) DNA must first be extracted and precisely cut so that it can be studied. Restriction enzymes (or molecular scissors) cut DNA at a certain nucleotide sequence called a restriction ...
Genetic Engineering
... A small, circular DNA molecule that carries genes separate from the main DNA of a bacterial cell ...
... A small, circular DNA molecule that carries genes separate from the main DNA of a bacterial cell ...
Molecular Genetics
... • 1 circular chromosome (plus optional plasmids, which are also circular) • reproduction usually asexual • sexual processes (mixing DNA from 2 individuals) occur, but with unequal contributions from the 2 partners • transcription and translation simultaneous ...
... • 1 circular chromosome (plus optional plasmids, which are also circular) • reproduction usually asexual • sexual processes (mixing DNA from 2 individuals) occur, but with unequal contributions from the 2 partners • transcription and translation simultaneous ...
Mutations
... keto and enol forms – In keto form, looks like T – In enol form looks like C – Used one way, but when copied, mispairing can occur. • Modifying agents: chemically change bases – HNO2 nitrous acid: deaminates (amino to keto) • See upcoming slide: deamination ...
... keto and enol forms – In keto form, looks like T – In enol form looks like C – Used one way, but when copied, mispairing can occur. • Modifying agents: chemically change bases – HNO2 nitrous acid: deaminates (amino to keto) • See upcoming slide: deamination ...
Chapter-9-Chromosomes-and-DNA-Replication
... Meiosis (more in Chapter 10) • This is the process by which a cell called the gamete mother cell found in the testes in men and ovaries in women undergo division to form 4 new sex cells ( sperm or egg) each containing 23 chromosomes. ...
... Meiosis (more in Chapter 10) • This is the process by which a cell called the gamete mother cell found in the testes in men and ovaries in women undergo division to form 4 new sex cells ( sperm or egg) each containing 23 chromosomes. ...
Sequencing the Human Genome
... 3. Take pairs of fragments, and match up the overlapping right- and left-hand ends letter by letter to grow longer and longer multifragment subsequences that are consistent with all of the contained fragments. ...
... 3. Take pairs of fragments, and match up the overlapping right- and left-hand ends letter by letter to grow longer and longer multifragment subsequences that are consistent with all of the contained fragments. ...
2017 DNA Lab Programmes Booklet
... “The instructor is knowledgeable and infused the lesson with many stories related to today's lesson. This piqued the pupils' interest in Science.” “Instructions given were very clear, simple and direct. In-depth learning and clarity of explanation by instructor.” “Very good instructor – very enthusi ...
... “The instructor is knowledgeable and infused the lesson with many stories related to today's lesson. This piqued the pupils' interest in Science.” “Instructions given were very clear, simple and direct. In-depth learning and clarity of explanation by instructor.” “Very good instructor – very enthusi ...
LN #23
... Change in a single base pair in DNA. The change results in an incorrect amino acid being added to the protein chain during translation. The change of one amino acid affects the shape of the entire protein. ...
... Change in a single base pair in DNA. The change results in an incorrect amino acid being added to the protein chain during translation. The change of one amino acid affects the shape of the entire protein. ...
Objective Questions
... 11) Which of the following proteins are not coded for by genes carried on plasmids? A) Enzymes necessary for conjugation B) Enzymes that catabolize hydrocarbons C) Bacteriocins D) Enzymes that inactivate antibiotics E) None of the above 12) Transformation is the transfer of DNA from a donor to a rec ...
... 11) Which of the following proteins are not coded for by genes carried on plasmids? A) Enzymes necessary for conjugation B) Enzymes that catabolize hydrocarbons C) Bacteriocins D) Enzymes that inactivate antibiotics E) None of the above 12) Transformation is the transfer of DNA from a donor to a rec ...
5.2.3 Genomes and Gene Technologies
... • Genes code for production of polypeptides and proteins • This coding DNA is only 1.5% of the whole genome • The rest is non-coding or ‘junk’ DNA • We still don’t know what this ‘junk’ DNA does and research is ongoing • Genomics = the study of genomes and the ‘mapping’ (finding out the role of each ...
... • Genes code for production of polypeptides and proteins • This coding DNA is only 1.5% of the whole genome • The rest is non-coding or ‘junk’ DNA • We still don’t know what this ‘junk’ DNA does and research is ongoing • Genomics = the study of genomes and the ‘mapping’ (finding out the role of each ...
DNA supercoil
DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.