Biotechnology
... Selection of Transformants • Hosts are chosen that are sensitive to a particular substance or require a particular nutrient (auxotrophs) • The vector provides the genes needed to be resistant to the substance or produce the nutrient • Host cells taking up vector or recombinant vector live • Host ce ...
... Selection of Transformants • Hosts are chosen that are sensitive to a particular substance or require a particular nutrient (auxotrophs) • The vector provides the genes needed to be resistant to the substance or produce the nutrient • Host cells taking up vector or recombinant vector live • Host ce ...
Assessment Statement
... IB says: Originally, it was assumed that one gene would invariably code for one polypeptide, but many exceptions have been discovered. ...
... IB says: Originally, it was assumed that one gene would invariably code for one polypeptide, but many exceptions have been discovered. ...
Repetitive DNA info - A. Prokaryotes Eukaryotes Most codes for
... Microsatellites are also known as short tandem repeats (STR), because a repeat unit consists of only 10 to 100 bp and the whole repetitive region spans less than 150 bp. Similar to minisatellites, the number of repeats for a given microsatellite may differ between individuals. Therefore, microsatell ...
... Microsatellites are also known as short tandem repeats (STR), because a repeat unit consists of only 10 to 100 bp and the whole repetitive region spans less than 150 bp. Similar to minisatellites, the number of repeats for a given microsatellite may differ between individuals. Therefore, microsatell ...
1 - Biology Mad
... Recently a strain of genetically engineered clover has been developed which has a high concentration of proteins rich in sulphur-containing amino-acids. A piece of DNA was prepared which contained the three different genes. This was inserted into a clover plant. Gene 1 obtained from sunflower seeds. ...
... Recently a strain of genetically engineered clover has been developed which has a high concentration of proteins rich in sulphur-containing amino-acids. A piece of DNA was prepared which contained the three different genes. This was inserted into a clover plant. Gene 1 obtained from sunflower seeds. ...
8.4 Lecture - Issaquah Connect
... strand of RNA nucleotides. (6) – The DNA helix winds again as the gene is transcribed. ...
... strand of RNA nucleotides. (6) – The DNA helix winds again as the gene is transcribed. ...
Nerve activates contraction
... B lymphocytes (WBC) produce immunoglobins, or antibodies, that specifically recognize and combat viruses, bacteria, and other invaders. -Millions of types of Antibodies can be produced depending on what the infectious agent is - how? -Immunoglobins have constant and variable region -100s of gene se ...
... B lymphocytes (WBC) produce immunoglobins, or antibodies, that specifically recognize and combat viruses, bacteria, and other invaders. -Millions of types of Antibodies can be produced depending on what the infectious agent is - how? -Immunoglobins have constant and variable region -100s of gene se ...
NAME: NWAIWU ROSEMARY DEPT: BIOCHEMISTRY COURSE
... chromosomes, and an extra chromosome can have a tremendous negative impact. For example, there should be two of chromosome 21, as with all other chromosomes, but if there are three, the result is Down syndrome. People with Down syndrome have a unique physical appearance and are developmentally disab ...
... chromosomes, and an extra chromosome can have a tremendous negative impact. For example, there should be two of chromosome 21, as with all other chromosomes, but if there are three, the result is Down syndrome. People with Down syndrome have a unique physical appearance and are developmentally disab ...
DNA-KRAMATİN VE KROMOZOM
... DNA Cloning 1. Purpose:- to amplify (bulk up) a small amount of DNA by inserting it into in a fast growing cell e.g. bacterium, so as bacterium divides we will have many copies of our DNA 2. 1. Obtain a DNA vector which can replicate inside a bacterial cell (plasmid or virus) which 3. 2. Insert DNA ...
... DNA Cloning 1. Purpose:- to amplify (bulk up) a small amount of DNA by inserting it into in a fast growing cell e.g. bacterium, so as bacterium divides we will have many copies of our DNA 2. 1. Obtain a DNA vector which can replicate inside a bacterial cell (plasmid or virus) which 3. 2. Insert DNA ...
Supplementary Information (doc 36K)
... purified and proteins removed from the solution by adding 7.5 M ammonium acetate (0.4x the final volume), placing the tubes on ice for 5 min, and centrifugation for 8 min at 15230 x g. The supernatant was transferred to a new reaction tube and a volume of isopropanol corresponding to 70% of the volu ...
... purified and proteins removed from the solution by adding 7.5 M ammonium acetate (0.4x the final volume), placing the tubes on ice for 5 min, and centrifugation for 8 min at 15230 x g. The supernatant was transferred to a new reaction tube and a volume of isopropanol corresponding to 70% of the volu ...
Medical School Biochemistry - Fall 2002
... resulting four DNA molecules? A. Half should contain no radioactivity. B. All should contain radioactivity. C. Half should contain radioactivity in both strands. D. One should contain radioactivity in both strands. E. None should contain radioactivity. ...
... resulting four DNA molecules? A. Half should contain no radioactivity. B. All should contain radioactivity. C. Half should contain radioactivity in both strands. D. One should contain radioactivity in both strands. E. None should contain radioactivity. ...
Document
... entangled in a process called synapsis. Crossing over can occur during this time. (Prophase I) (It is the Homologous pair that is pulled apart during Anaphase I) ...
... entangled in a process called synapsis. Crossing over can occur during this time. (Prophase I) (It is the Homologous pair that is pulled apart during Anaphase I) ...
Restriction enzyme
... It is a fragment of DNA of variable length (usually 100-1000 bases long), which is used to detect in DNA the presence of nucleotide sequences that are complementary to the sequence in the probe Must be labeled to be visualized Usually prepared by making a radioactive copy of a DNA fragment. Probing ...
... It is a fragment of DNA of variable length (usually 100-1000 bases long), which is used to detect in DNA the presence of nucleotide sequences that are complementary to the sequence in the probe Must be labeled to be visualized Usually prepared by making a radioactive copy of a DNA fragment. Probing ...
VGEC: Student Handout Wear a Chimp on Your Wrist 1
... Almost all life on earth gets energy from food in the same way, which is why this gene is found in everything from plants to humans. As you can see, the DNA sequence of the gene doesn’t need to be the same for the protein produced from it to do the same job. However, more closely related animals do ...
... Almost all life on earth gets energy from food in the same way, which is why this gene is found in everything from plants to humans. As you can see, the DNA sequence of the gene doesn’t need to be the same for the protein produced from it to do the same job. However, more closely related animals do ...
Beyond Mendel: Molecular genetics, cell division, and sex
... − the shape is largely determined by the sequence of amino acids: the protein’s primary structure − because that affects how and where the chain can bend, stick to itself, and so on − Proteins have several kinds of functions − some are structural proteins, which form the physical structure of the bo ...
... − the shape is largely determined by the sequence of amino acids: the protein’s primary structure − because that affects how and where the chain can bend, stick to itself, and so on − Proteins have several kinds of functions − some are structural proteins, which form the physical structure of the bo ...
AP Biology: Evolution
... 2. Using the ideal gel shown in Figure 5, measure the distance (in cm) that each fragment migrated from the origin (the well). (Hint: For consistency, measure from the front end of each well to the front edge of each band, i.e., the edge farthest from the well.). Enter the measured distances into Ta ...
... 2. Using the ideal gel shown in Figure 5, measure the distance (in cm) that each fragment migrated from the origin (the well). (Hint: For consistency, measure from the front end of each well to the front edge of each band, i.e., the edge farthest from the well.). Enter the measured distances into Ta ...
Making probes/primers
... DNA synthesis using the Phosphoramidite method. •Before the start of synthesis amino groups of adenine, guanine and cytosine are derivatised by addition of benzoyl, isobutyryl and benzoyl groups respectively to prevent undesirable side reactions during chain growth. •Thymine is not treated as it ha ...
... DNA synthesis using the Phosphoramidite method. •Before the start of synthesis amino groups of adenine, guanine and cytosine are derivatised by addition of benzoyl, isobutyryl and benzoyl groups respectively to prevent undesirable side reactions during chain growth. •Thymine is not treated as it ha ...
Spring 2015-Chapter 8
... called methylmercury. This compound can accumulate in the tissue of fish or shellfish. According to the Environmental Protection Agency (EPA), almost all methylmercury exposures in the US stem from consuming fish or shellfish that contain high levels of the compound. Ingesting high levels of mercury ...
... called methylmercury. This compound can accumulate in the tissue of fish or shellfish. According to the Environmental Protection Agency (EPA), almost all methylmercury exposures in the US stem from consuming fish or shellfish that contain high levels of the compound. Ingesting high levels of mercury ...
ch11dna - cpolumbo
... STR is another method of DNA typing. STR’s are locations (loci) on the chromosome that contain short sequences of 2 to 5 bases that repeat themselves in the DNA molecule. The advantages of this method are that it provides greater discrimination, requires less time, a smaller sample size, and the DNA ...
... STR is another method of DNA typing. STR’s are locations (loci) on the chromosome that contain short sequences of 2 to 5 bases that repeat themselves in the DNA molecule. The advantages of this method are that it provides greater discrimination, requires less time, a smaller sample size, and the DNA ...
DNA WebQuest
... 7) What are some other proteins that genes code for? Click on “What is a chromosome?” at the top and go through the animation. Answer the questions. 8) How long would the DNA in one human cell be? 9) How is DNA packaged to fit into the small space of a cell nucleus? 10) How many chromosomes are in a ...
... 7) What are some other proteins that genes code for? Click on “What is a chromosome?” at the top and go through the animation. Answer the questions. 8) How long would the DNA in one human cell be? 9) How is DNA packaged to fit into the small space of a cell nucleus? 10) How many chromosomes are in a ...
Nucleic acids - Haiku Learning
... on the outside of the helix The nitrogen bases form the flat inner rungs (steps on the ladder) of DNA If you know the order of Nitrogen bases on one strand of DNA, you can determine the other: Practice: ACTTGCCA Answer: TGAACGGT ...
... on the outside of the helix The nitrogen bases form the flat inner rungs (steps on the ladder) of DNA If you know the order of Nitrogen bases on one strand of DNA, you can determine the other: Practice: ACTTGCCA Answer: TGAACGGT ...
DNA supercoil
DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.