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Name_______________________ Period___________ Chapter
Name_______________________ Period___________ Chapter

... Figure 20.4 in your text is a more detailed discussion of the gene cloning procedure shown in Figure 20.2. Explain the following key points. a. What is the source of the gene of interest? A particular species of hummingbird b. Explain why the plasmid is engineered with ampR and lacZ. AmpR is used be ...
Biology_ch_11_genetics - Miami Beach Senior High School
Biology_ch_11_genetics - Miami Beach Senior High School

... for a protein, which in turn codes for a trait (skin tone, eye color.etc),  a gene is a stretch of DNA.  Every person has two copies of each gene, one inherited from each parent. ...
Chapter 20: Biotechnology - Staff Web Sites @ BBHCSD
Chapter 20: Biotechnology - Staff Web Sites @ BBHCSD

... Figure 20.4 in your text is a more detailed discussion of the gene cloning procedure shown in Figure 20.2. Explain the following key points. a. What is the source of the gene of interest? A particular species of hummingbird b. Explain why the plasmid is engineered with ampR and lacZ. AmpR is used be ...
Review Sheet : DNA, RNA & Protein Synthesis
Review Sheet : DNA, RNA & Protein Synthesis

... Which of the following is not true about DNA replication?  a. It must occur before a cell can divide  b. Two complementary strands are duplicated.  c. The double strand unwinds and unzips while it is being duplicated.  d. The process is catalyzed by enzymes called DNA mutagens. ...
10. Genetic engineering and bacteria
10. Genetic engineering and bacteria

... • Bacterial plasmids are often used as vectors – plasmids are small, circular pieces of DNA found in many bacteria types. • Plasmids are separate from main chromosomes and often carry genes for ...
RNA PP
RNA PP

... to DNA and separates the DNA strands. RNA polymerase then uses one strand of DNA as a template from which nucleotides are assembled into a strand of RNA. • So, RNA is making a single-stranded copy from DNA that takes information out of the nucleus. ...
Forensic Science – Study Guide for Final (Spring 2013)
Forensic Science – Study Guide for Final (Spring 2013)

... i. Is blood type class vs. individual evidence? How is it used ii. Types: A, B, O, AB; Rh factor 1. What are the antigens associated with each type? Where are they located? 2. What are the antibodies associated with each type? 3. What is the proportion of each type(%) in the human population? 4. How ...
Wanganui High School
Wanganui High School

... In mitosis what structure breaks down before the chromosomes line up on the equator? ...
Unit 6: Biotechnology
Unit 6: Biotechnology

... 1. Plasmids or viruses are then used as a vector, a genetic vehicle that carries foreign DNA into a host cell. Usually, the host cell is a ____Bacterium___. 2. The recombinant DNA inside the host cell reproduces new cells that contain copies of the inserted gene. F. Screening – extracting copied gen ...
F: Acronyms and Glossary
F: Acronyms and Glossary

... reproduces a specific region of DNA between two sites, yielding millions of copies from the original. Polymorphism: The existence of more than one form of a genetic trait. Preexisting condition: A condition existing before an insurance policy goes into effect and commonly defined as one which would ...
Transcription PPT
Transcription PPT

DNA: the thread of life
DNA: the thread of life

... phage), which only infects bacterial cells. At that time, scientists knew that when these phage infect a bacterial cell, they somehow “reprogram” the bacterium to transform itself into a factory for producing more phage. • They also knew that the phage itself does not enter the bacterium during an i ...
Cells
Cells

... magnification only during certain phases of cell division. ...
Protein Synthesis
Protein Synthesis

... 17. UAG is a stop codon. What might happen if the uracil in this codon was changed to cytosine? Glutamine would have been added to the polypeptide chain. 18. List the four different sets of DNA nucleotide sequences that code for the amino acid Valine. Explain why this redundancy in the genetic code ...
Lecture 7
Lecture 7

... step, the XPC-hHR23B complex recognizes the damage (a pyrimidine dimer in this case), binds to it, and causes localized DNA melting. XPA also aids this process. RPA binds to the undamaged DNA strand across from the damage. (b) The DNA helicase activity of TFIIH causes increased DNA melting. (c) RPA ...
Nucleic Acids
Nucleic Acids

... DNA replication “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material.” James Watson Francis Crick ...
Lecture 10 Types of mutations Substitutions that occur in protein
Lecture 10 Types of mutations Substitutions that occur in protein

... Mutation Rate per bp ...
Introduction - OpenWetWare
Introduction - OpenWetWare

... only the “correct” ends will come together, if we have chosen our restriction enzymes appropriately. However, after the temporary base-pairs form, the sugar-phosphate backbones of the two pieces of DNA are still separate, and they may be pushed apart by other molecules moving around. The ligase enzy ...
Unit 5 DNA/RNA/PROTEIN SYNTHESIS
Unit 5 DNA/RNA/PROTEIN SYNTHESIS

... Translation is the process of interpreting the genetic message and building the protein and begins when the mRNA attaches to a ribosome, which contains proteins and ribosomal RNA (rRNA), in the cytoplasm.  The function of ribosomes is to assemble proteins according to the code that the mRNA brings ...
Electrical Biosensors in Microfluidic for High Throughput Genomics and Proteomics   
Electrical Biosensors in Microfluidic for High Throughput Genomics and Proteomics   

... Electrical Biosensors in Microfluidic for High Throughput Genomics and Proteomics  ...
Inquiry into Life Twelfth Edition
Inquiry into Life Twelfth Edition

... • In first step, 2 plasmids fuse, phage replication, forms a cointegrate – coupled through pair of Tn3 copies • Next is resolution of cointegrate, breaks down into 2 independent plasmids, catalyzed by resolvase gene ...
Genetic Terminology
Genetic Terminology

BLOTTING TECHNIQUES - University of Kufa
BLOTTING TECHNIQUES - University of Kufa

... Professor Sir Edwin Southern, Professor of Biochemistry and Fellow of Trinity developed this method in 1975. Southern won the Lasker Award for Clinical Medical Research prize for the method of finding specific DNA sequences he developed this procedure at Edinburgh University more than 30 years ago. ...
Single Nucleotide Polymorphism
Single Nucleotide Polymorphism

Genetics Test 1 Review
Genetics Test 1 Review

... Discuss the events in a cell during prophase II and metaphase II in meiosis. Make special mention of any important differences between meiosis and mitosis at these times. ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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